Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913599,5760TG51.3% 104.0 / 131.0 77V288G (GTG→GGG) cusAcopper/silver efflux system, membrane component
Reads supporting (aligned to +/- strand):  ref base T (4/33);  new base G (39/0);  total (44/33)
Fisher's exact test for biased strand distribution p-value = 1.84e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.85e-13

CGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATCGACAACC  >  NC_000913/599429‑599725
                                                                                                                                                   |                                                                                                                                                      
cGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGggtgg                                                                                                                                                      <  1:475906/150‑1 (MQ=255)
    cgacgaCTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGa                                                                                                                                                   <  2:11028/149‑1 (MQ=255)
     gacgacTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATcc                                                                                                                                                <  2:541842/151‑1 (MQ=255)
      acgacTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGTGAAcc                                                                                                                                                >  2:1270892/1‑150 (MQ=255)
      acgacTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATcc                                                                                                                                                >  2:875027/1‑150 (MQ=255)
         acTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCg                                                                                                                                           <  2:1089561/151‑1 (MQ=255)
         acTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTcc                                                                                                                                                >  2:326512/1‑147 (MQ=255)
           tttAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGCAGTGGCGGGGGGGGGGGGGGTGCTGCg                                                                                                                                           >  1:1055905/1‑149 (MQ=255)
             tAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGAtac                                                                                                                                                >  1:89660/1‑141 (MQ=255)
             tAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATc                                                                                                                                        <  1:1100345/150‑1 (MQ=255)
              aaTCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCg                                                                                                                                      <  1:50444/151‑1 (MQ=255)
                 cacaTCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGTGATCCTGCCATCCCGc                                                                                                                                    >  2:182246/1‑150 (MQ=255)
                   caTCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCTGCGATCCGGCaa                                                                                                                                  >  2:947560/1‑150 (MQ=255)
                    aTCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGTGTCCCGCGATCCGGCcaa                                                                                                                                 >  2:628440/1‑150 (MQ=255)
                    aTCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTCCTGCGGTCCGGCaaa                                                                                                                                 >  2:349203/1‑150 (MQ=255)
                        ttttAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGTGATCCCGCGATACGGCAAAAAAGc                                                                                                                            >  2:709503/1‑151 (MQ=255)
                         tttAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTCCCGCGGCCCCGCCAAAACAcc                                                                                                                           >  1:137994/1‑151 (MQ=255)
                           tAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCTGCGATCCGGCAAAAACGccc                                                                                                                          >  2:1160831/1‑150 (MQ=255)
                             aaaGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGTGGGGGTCCTGGCGACCCGGCaaaacacccggg                                                                                                                        >  2:336850/1‑143 (MQ=255)
                             aaaGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTCCTGCGGTCCCGCAAAAAAGcc                                                                                                                           >  2:464708/1‑147 (MQ=255)
                             aaaGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCCGCGATCCGGCAAAAACGcccccg                                                                                                                       >  2:626157/1‑148 (MQ=255)
                               aGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTCCTGCGATCCGGGAAAAACGCCCcag                                                                                                                       >  2:859699/1‑149 (MQ=255)
                                  cAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTg                                                                                                                  <  1:113199/151‑1 (MQ=255)
                                    gTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGAt                                                                                                                <  2:1341164/151‑1 (MQ=255)
                                       aaaaTGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATcgc                                                                                                             <  2:983210/151‑1 (MQ=255)
                                          aTGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTTCTGCGATCCGGCAAAAACGCCCGGGAAGGGATc                                                                                                               >  2:840494/1‑146 (MQ=255)
                                          aTGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCTGCGGTCCGGCGAAAACACCCGAGAAGGGATtgcc                                                                                                            >  2:844596/1‑149 (MQ=255)
                                              cGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTg                                                                                                      <  2:1265517/151‑1 (MQ=255)
                                               gTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGa                                                                                                     <  2:212790/151‑1 (MQ=255)
                                               gTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGTGGTCCTGCGCTCCCGCAAAAACGCCCGAGAAGGGATCGCCGCCGTg                                                                                                      >  1:667732/1‑150 (MQ=255)
                                                 gCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTCCTGCGATCCCGCAAAAACGCCCCAGAAGTGAACGCCGCCGTg                                                                                                      >  2:919811/1‑148 (MQ=255)
                                                   ccGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGa                                                                                                 <  2:1333589/151‑1 (MQ=255)
                                                    cGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCAGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCTGCGATCCGGCAAAAAAGCCCGAGAAGGGATCGCCGCCGTGAAGGa                                                                                                 >  2:862586/1‑150 (MQ=255)
                                                     gTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGTGATCCTGCGATCCGGCAAAAAAGCCCGAGAAGGGATCGCCGCCGTGAAgg                                                                                                  >  2:907082/1‑148 (MQ=255)
                                                     gTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGGCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGGGAAgg                                                                                                  >  2:40696/1‑148 (MQ=255)
                                                       ttATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCCGCGCTCCGGCAAAAACGCCCGCGAAGTGATCGCCGCCGGGAAGGGCaa                                                                                              >  1:171454/1‑150 (MQ=255)
                                                        tATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCTGCGATCCGGCAAAAACGCCCGGGAAGGGATCGCCGCCGGGGAGGACaaa                                                                                             >  2:1077516/1‑150 (MQ=255)
                                                          tcggcgcGATGTTGCGAAGGTCCAGATTGGCCCGGGGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAAGCGAAGTGGCGTGCGGGGTGGTTGTCCTGCGATCCGGCAAAAACCCCCGAGAAGTGATCGCCGCTGTGAAGTACAAACt                                                                                           <  1:693632/147‑1 (MQ=255)
                                                           cTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTCCTGCGATCCGGCCAAAAAGCCCGGGAAGTGATCGCCGCCGTGAAGGACAAACTgg                                                                                         >  2:722707/1‑151 (MQ=255)
                                                                cgATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACg                                                                                    <  2:619143/151‑1 (MQ=255)
                                                                         gAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAagt                                                                           <  1:65033/151‑1 (MQ=255)
                                                                         gAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAag                                                                            <  1:854058/150‑1 (MQ=255)
                                                                         gAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCTGCGATCCGGCAAAAACACCCCAGGAGTGATCGCCGCCGGGAAGGGCAAACTGGAAAACCCGaaaa                                                                             >  1:850074/1‑149 (MQ=255)
                                                                               ccAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCt                                                                      <  1:309455/150‑1 (MQ=255)
                                                                                  gATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAAAGCTGAAAAGTAGTCTGcc                                                                   >  2:1122590/1‑150 (MQ=255)
                                                                                  gATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCTGCGATCCGGCAAAAACCCCCGAGAAGGGATCGCCGCCGGGAAGGACAAACTGGAAAAGCTGAAAAGGAGGCTGcc                                                                   >  2:713271/1‑150 (MQ=255)
                                                                                    ttGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGGGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCgg                                                                 <  1:505069/150‑1 (MQ=255)
                                                                                      ggCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCTGCGATCCGGCAAAAAAGCCCGAGAAGGGATCGCCGCCGGGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCgg                                                                 >  1:1185165/1‑148 (MQ=255)
                                                                                        cccGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGc                                                            <  2:137633/151‑1 (MQ=255)
                                                                                           ggAGATGCTCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTg                                                         <  1:965013/151‑1 (MQ=255)
                                                                                           ggAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTg                                                         <  1:867064/151‑1 (MQ=255)
                                                                                           ggAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGGGAAGGACAAACTGGAAACGCTGAAAAGAAGTCTGCCGGAAGGCggg                                                         >  2:662893/1‑149 (MQ=255)
                                                                                                 gcgcCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGCATCTGCCATCCGGGAAAAACGGCCGCGGAGGGATTGCCCCCCGGAAGGGCCAACTGGGAAACCCGAAAAGTAGGCTGCCGGGAggggggg                                                        >  1:349207/1‑141 (MQ=255)
                                                                                                  cgcCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCCGCGGTCCGGCAAAAACGCCCGGGAAGGGATCGCCGCCGCGAAGGACAAACTGGAAACGCTGAAAAGGAGTCTGGCGGGAGGCGGGggga                                                     >  1:488197/1‑148 (MQ=255)
                                                                                                   gcCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAg                                                  <  1:15247/150‑1 (MQ=255)
                                                                                                   gcCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGt                                                 <  2:1332631/151‑1 (MQ=255)
                                                                                                   gcCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGt                                                 <  2:1039798/151‑1 (MQ=255)
                                                                                                   gcCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGt                                                 <  1:65586/151‑1 (MQ=255)
                                                                                                    ccGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGAGGGGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGGCAAAATGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATa                                                   >  2:93088/1‑148 (MQ=255)
                                                                                                     cGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTCCTGCGGTCCCGCAAAAAACCCCGGGAAGTGATCGCCGCCGCGGAGGACAAACTGGAAACGCTGGAAAGGAGTCTGGCGGGAGGCGCGGGGATAGtt                                                >  1:927200/1‑150 (MQ=255)
                                                                                                      ggggCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGGGATCCTGCGATCCGGCAAAAAAGCCCGCGAAGGGATCGCCCCCGTGAAGGACAAACTGGAAACGCTGGAAAGGAGTCTGCCGGAAGGCGGGGAGATAGtt                                                >  1:1194611/1‑149 (MQ=255)
                                                                                                      ggggCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTCCTGCGATCCGGCCAAAACGCCCGAGAAGGGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGGAGGCGTGGAGGTAGtt                                                >  1:915374/1‑149 (MQ=255)
                                                                                                        ggCATTGCCGAATTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTacaa                                            <  1:1059659/151‑1 (MQ=255)
                                                                                                         gCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCTGCGATCCGGCAAAAAAGCCCGGGAAGGGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGGAGGCGGGGGGATAGTTacaa                                            >  2:348258/1‑150 (MQ=255)
                                                                                                          cATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTacaaca                                          <  2:650999/151‑1 (MQ=255)
                                                                                                            ttGCCGAACTAAACGGCGAAGGCGATGTGGCGGGCGGGGGGGGGATCCCGCGATCCGGCAAAAACCCCCGAGGAGGGGTCGCCGCCCGGAAAGAAAAACAGGGAACGCTGAAAAGTAGTCTGCCGGAAGGCGGGGGGATAGTTaataca                                          >  1:1264406/1‑144 (MQ=255)
                                                                                                              gCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATAc                                       <  1:818325/150‑1 (MQ=255)
                                                                                                              gCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACg                                      <  1:947429/151‑1 (MQ=255)
                                                                                                                  aaCTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCg                                  <  2:58761/151‑1 (MQ=255)
                                                                                                                  aaCTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGTGATCCTGCGATCCGGCAAAAAAGCCCGAGAAGGGATCGCCGCCGGGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGGGGGGATAGGTACAACATACGATCg                                  >  2:112169/1‑151 (MQ=255)
                                                                                                                         cGGCGAAGGCGAAGTGGCGGGCGGGGTGGGGGTCCTGCGATCCGGCAAAAACGCCCGAGGAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGGAGGCTGCCGGAAGGCGGGGGGGTAGGTACAACATACGATCGcagc                              >  2:478164/1‑148 (MQ=255)
                                                                                                                         cGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCTGCGGTCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGGGAAGGACAAACTGGAAACGCTGAAAAAGAGTCTGCCGGGAGGAGTGGGGATAGTTACAACAAACGATCGcagcc                             >  1:905810/1‑149 (MQ=255)
                                                                                                                           gcgaaggcgaagTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCt                         <  2:866406/151‑1 (MQ=255)
                                                                                                                            cgaaggcgaagTGGCGGGCGGGGGGGTGATCCTCCGATCCGGCAAAAACGCCCGCGAAGAGATCGCCGGCGTGAAGGAGAAAATGGAAAAGCTGAAAAGGAACCTGCCGGAAGGCGGGGAGATAGGTAAAAAATAAGATCGCAGCCAGGTc                        >  1:366175/1‑151 (MQ=255)
                                                                                                                                    aagTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACcg                <  2:1227059/151‑1 (MQ=255)
                                                                                                                                           gggcgggGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCAt          <  2:1096373/150‑1 (MQ=255)
                                                                                                                                           gggcgggGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATc         <  2:142329/151‑1 (MQ=255)
                                                                                                                                                gggTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATCGACa     >  2:1340433/1‑150 (MQ=255)
                                                                                                                                                 ggtggtGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATCGACAAc   <  1:984115/151‑1 (MQ=255)
                                                                                                                                                  gtggtgATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATCGACAAcc  <  2:168122/151‑1 (MQ=255)
                                                                                                                                                  gtggtgATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATCGACAAcc  >  1:1096566/1‑151 (MQ=255)
                                                                                                                                                   |                                                                                                                                                      
CGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATCGACAACC  >  NC_000913/599429‑599725

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: