Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,352,926	C→T	G235S (GGT→AGT)	sapD ←	antimicrobial peptide ABC transporter ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,352,926	0	C	T	100.0%	310.3 / NA	80	G235S (GGT→AGT)	sapD	antimicrobial peptide ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (35/45);  total (35/45)

AGTGGGATAGCGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGCGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACCGCAGTAAAGCACGTTAATTTTATCCGCCCATTGGCTAAGCATTTGTAAGTCATGGCTGATAAGCAAAATAGTGGTATTGCTGTTTTGGTTGAGACGCGTCAGCAGGCGAAAGATTTGCGCCTGGGTTGTTGGCTCCATTGAGTTGG  >  NC_000913/1352778‑1353072                                                                                                                                                     |                                                                                                                                                   agTGGGATAGCGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGCGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACTGc                                                                                                                                                  >  1:266722/1‑151 (MQ=255)      gATAGCGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGCGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACTGCAGTaa                                                                                                                                             <  1:802751/151‑1 (MQ=255)         aGCGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGCGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACTGCAGTAAAg                                                                                                                                           <  1:568987/150‑1 (MQ=255)               gggCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGCGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACTGCAGTAAAGCACGTTa                                                                                                                                    <  2:1337384/151‑1 (MQ=255)                ggCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGCGCCTGGGTATAAGGATGATGTGGCATCGTCCCCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACTGCAGTAAAGCACGtt                                                                                                                                     <  2:1290947/149‑1 (MQ=255)                ggCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGCGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACTGCAGTAAAGCACGTTaa                                                                                                                                   <  1:1206162/151‑1 (MQ=255)                  cAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGCGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACTGCAGTAAAGCACGTTAAtt                                                                                                                                 <  1:713664/151‑1 (MQ=255)                   aGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGCGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACTGCAGTAAAGCACGTTAAttt                                                                                                                                <  1:13491/151‑1 (MQ=255)                    gCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGCGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACTGCAGTAAAGCACGTTAAtttt                                                                                                                               <  1:312789/151‑1 (MQ=255)                      gTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGCGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACTGCAGTAAAGCACGTTAATTTTAt                                                                                                                             >  2:602631/1‑151 (MQ=255)                      gTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGCGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACTGCAGTAAAGCACGTTAATTTTAt                                                                                                                             <  2:743855/151‑1 (MQ=255)                        aTTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGCGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACTGCAGTAAAGCACGTTAATTTTATcc                                                                                                                           >  1:952540/1‑151 (MQ=255)                         ttGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGCGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACTGCAGTAAAGCACGTTAATTTTATCCg                                                                                                                          >  1:1013014/1‑151 (MQ=255)                          tGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGCGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACTGCAGTAAAGCACGTTAATTTTATCCGc                                                                                                                         >  1:221203/1‑151 (MQ=255)                          tGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGCGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACTGCAGTAAAGCACGTTAATTTTATCCGc                                                                                                                         >  2:3358/1‑151 (MQ=255)                           gAGGCTACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGCGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACTGCAGTAAAGCACGTTAATTTTATCCGcc                                                                                                                        >  1:275570/1‑151 (MQ=255)                                gACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGCGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACTGCAGTAAAGCACGTTAATTTTATCCGCCCATTg                                                                                                                   >  2:630572/1‑151 (MQ=255)                                   ttttATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGCGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACTGCAGTAAAGCACGTTAATTTTATCCGCCCATTGGCt                                                                                                                >  2:1311364/1‑151 (MQ=255)                                     ttATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGCGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACTGCAGTAAAGCACGTTAATTTTATCCGCCCATTGGCTa                                                                                                               <  1:458074/150‑1 (MQ=255)                                       aTGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGCGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACTGCAGTAAAGCACGTTAATTTTATCCGCCCATTGGCTAAGc                                                                                                            >  2:1343638/1‑151 (MQ=255)                                        tGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGCGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACTGCAGTAAAGCACGTTAATTTTATCCGCCCATTGGCTAAGc                                                                                                            >  2:274443/1‑150 (MQ=255)                                         gCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGCGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACTGCAGTAAAGCACGTTAATTTTATCCGCCCATTGGCTAAGCa                                                                                                           <  1:363953/150‑1 (MQ=255)                                         gCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGCGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACTGCAGTAAAGCACGTTAATTTTATCCGCCCATTGGCTAAGCAt                                                                                                          >  2:653346/1‑151 (MQ=255)                                         gCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGCGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACTGCAGTAAAGCACGTTAATTTTATCCGCCCATTGGCTAAGCAt                                                                                                          >  1:437094/1‑151 (MQ=255)                                          cGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGCGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACTGCAGTAAAGCACGTTAATTTTATCCGCCCATTGGCTAAGCAtt                                                                                                         <  1:1157299/151‑1 (MQ=255)                                             cATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGCGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACTGCAGTAAAGCACGTTAATTTTATCCGCCCATTGGCTAAGCATTTGt                                                                                                      <  2:675981/151‑1 (MQ=255)                                               tCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGCGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACTGCAGTAAAGCACGTTAATTTTATCCGCCCATTGGCTAAGCATTTGTa                                                                                                     >  2:1219330/1‑150 (MQ=255)                                                  cgcTGCCGAAGTCTGGTATCGCGCGGATCAGCGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACTGCAGTAAAGCACGTTAATTTTATCCGCCCATTGGCTAAGCATTTGTAAGTc                                                                                                 <  2:618242/151‑1 (MQ=255)                                                      gCCGAAGTCTGGTATCGCGCGGATCAGCGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACTGCAGTAAAGCACGTTAATTTTATCCGCCCATTGGCTAAGCATTTGTAAGTCATg                                                                                              <  2:192503/150‑1 (MQ=255)                                                       ccGAAGTCTGGTATCGCGCGGATCAGCGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACTGCAGTAAAGCACGTTAATTTTATCCGCCCATTGGCTAAGCATTTGTAAGTCATGGc                                                                                            <  2:751532/151‑1 (MQ=255)                                                        cGAAGTCTGGTATCGCGCGGATCAGCGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACTGCAGTAAAGCACGTTAATTTTATCCGCCCATTGGCTAAGCATTTGTAAGTCATGGCt                                                                                           >  1:1131596/1‑151 (MQ=255)                                                         gAAGTCTGGTATCGCGCGGATCAGCGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACTGCAGTAAAGCACGTTAATTTTATCCGCCCATTGGCTAAGCATTTGTAAGTCATGGCTg                                                                                          <  2:149192/151‑1 (MQ=255)                                                           aGTCTGGTATCGCGCGGATCAGCGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACTGCAGTAAAGCACGTTAATTTTATCCGCCCATTGGCTAAGCATTTGTAAGTCATGGCTGAt                                                                                        <  2:1308896/151‑1 (MQ=255)                                                            gTCTGGTATCGCGCGGATCAGCGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACTGCAGTAAAGCACGTTAATTTTATCCGCCCATTGGCTAAGCATTTGTAAGTCATGGCTGATa                                                                                       <  1:121990/151‑1 (MQ=255)                                                                ggTATCGCGCGGATCAGCGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACTGCAGTAAAGCACGTTAATTTTATCCGCCCATTGGCTAAGCATTTGTAAGTCATGGCTGATAAGc                                                                                    <  1:787303/150‑1 (MQ=255)                                                                ggTATCGCGCGGATCAGCGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACTGCAGTAAAGCACGTTAATTTTATCCGCCCATTGGCTAAGCATTTGTAAGTCATGGCTGATAAGCa                                                                                   <  2:465451/151‑1 (MQ=255)                                                                ggTATCGCGCGGATCAGCGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACTGCAGTAAAGCACGTTAATTTTATCCGCCCATTGGCTAAGCATTTGTAAGTCATGGCTGATAAGCa                                                                                   <  1:895424/151‑1 (MQ=255)                                                                   aTCGCGCGGATCAGCGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACTGCAGTAAAGCACGTTAATTTTATCCGCCCATTGGCTAAGCATTTGTAAGTCATGGCTGATAAGCaaa                                                                                 <  1:47482/150‑1 (MQ=255)                                                                        gcgGATCAGCGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACTGCAGTAAAGCACGTTAATTTTATCCGCCCATTGGCTAAGCATTTGTAAGTCATGGCTGATAAGCAAAATAGTg                                                                           <  2:327416/151‑1 (MQ=255)                                                                          ggATCAGCGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACTGCAGTAAAGCACGTTAATTTTATCCGCCCATTGGCTAAGCATTTGTAAGTCATGGCTGATAAGCAAAATAGTGGt                                                                         >  2:187306/1‑151 (MQ=255)                                                                           gATCAGCGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACTGCAGTAAAGCACGTTAATTTTATCCGCCCATTGGCTAAGCATGTGTAAGTCATGGCTGATAAGCAAAATAGTGGTa                                                                        <  2:185665/151‑1 (MQ=255)                                                                              cAGCGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACTGCAGTAAAGCACGTTAATTTTATCCGCCCATTGGCTAAGCATTTGTAAGTCATGGCTGATAAGCAAAATAGTGGTAtt                                                                      >  1:92228/1‑150 (MQ=255)                                                                              cAGCGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACTGCAGTAAAGCACGTTAATTTTATCCGCCCATTGGCTAAGCATTTGTAAGTCATGGCTGATAAGCAAAATAGTGGTAtt                                                                      <  2:1328264/150‑1 (MQ=255)                                                                              cAGCGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACTGCAGTAAAGCACGTTAATTTTATCCGCCCATTGGCTAAGCATTTGTAAGTCATGGCTGATAAGCAAAATAGTGGTATTg                                                                     <  2:773614/151‑1 (MQ=255)                                                                               aGCGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACTGCAGTAAAGCACGTTAATTTTATCCGCCCATTGGCTAAGCATTTGTAAGTCATGGCTGATAAGCAAAATAGTGGTATTGc                                                                    >  1:654254/1‑151 (MQ=255)                                                                               aGCGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACTGCAGTAAAGCACGTTAATTTTATCCACCCATTGGCTAAGCATTTGTAAGTCATGGCTGATAAGCAAAATAGTGGTATTGc                                                                    >  1:689819/1‑151 (MQ=255)                                                                                 cgcCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACTGCAGTAAAGCACGTTAATTTTATCCGCCCATTGGCTAAGCATTTGTAAGTCATGGCTGATAAGCAAAATAGTGGTATTGCTg                                                                  >  1:63891/1‑151 (MQ=255)                                                                                  gcCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACTGCAGTAAAGCACGTTAATTTTATCCGCCCATTGGCTAAGCATTTGTAAGTCATGGCTGATAAGCAAAATAGTGGTATTGCTGt                                                                 >  2:95530/1‑151 (MQ=255)                                                                                    cTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACTGCAGTAAAGCACGTTAATTTTATCCGCCCATTGGCTAAGCATTTGTAAGTCATGGCTGATAAGCAAAATAGTGGTATTGCTGttt                                                               >  2:143779/1‑151 (MQ=255)                                                                                     tGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACTGCAGTAAAGCACGTTAATTTTATCCGCCCATTGGCTAAGCATTTGTAAGTCATGGCTGATAAGCAAAATAGTGGTATTGCTGtttt                                                              >  2:589925/1‑151 (MQ=255)                                                                                      gggTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACTGCAGTAAAGCACGTTAATTTTATCCGCCCATTGGCTAAGCATTTGTAAGTCATGGCTGATAAGCAAAATAGTGGTATTGCTGtttt                                                              <  2:591239/150‑1 (MQ=255)                                                                                      gggTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACTGCAGTAAAGCACGTTAATTTTATCCGCCCATTGGCTAAGCATTTGTAAGTCATGGCTGATAAGCAAAATAGTGGTATTGCTGTTTTg                                                             >  1:593944/1‑151 (MQ=255)                                                                                       ggTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACTGCAGTAAAGCACGTTAATTTTATCCGCCCATTGGCTAAGCATTTGTAAGTCATGGCTGATAAGCAAAATAGTGGTATTGCTGTTTTgg                                                            >  1:34616/1‑151 (MQ=255)                                                                                         tataAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACTGCAGTAAAGCACGTTAATTTTATCCGCCCATTGGCTAAGCATTTGTAAGTCATGGCTGATAAGCAAAATAGTGGTATTGCTGTTTTGGtt                                                          >  2:305588/1‑151 (MQ=255)                                                                                          ataAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACTGCAGTAAAGCACGTTAATTTTATCCGCCCATTGGCTAAGCATTTGTAAGTCATGGCTGATAAGCAAAATAGTGGTATTGCTGTTTTGGtt                                                          <  2:938868/150‑1 (MQ=255)                                                                                          ataAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACTGCAGTAAAGCACGTTAATTTTATCCGCCCATTGGCTAAGCATTTGTAAGTCATGGCTGATAAGCAAAATAGTGGTATTGCTGTTTTGGTTg                                                         >  1:1080112/1‑151 (MQ=255)                                                                                           taAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACTGCAGTAAAGCACGTTAATTTTATCCGCCCATTGGCTAAGCATTTGTAAGTCATGGCTGATAAGCAAAATAGTGGTATTGCTGTTTTGGTTg                                                         <  1:1203381/150‑1 (MQ=255)                                                                                           taAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACTGCAGTAAAGCACGTTAATTTTATCCGCCCATTGGCTAAGCATTTGTAAGTCATGGCTGATAAGCAAAATAGTGGTATTGCTGTTTTGGTTg                                                         >  1:984540/1‑150 (MQ=255)                                                                                                atgatgTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACTGCAGTAAAGCACGTTAATTTTATCCGCCCATTGGCTAAGCATTTGTAAGTCATGGCTGATAAGCAAAATAGTGGTATTGCTGTTTTGGTTGAGAcgc                                                   <  2:977262/151‑1 (MQ=255)                                                                                                     gtgGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACTGCAGTAAAGCACGTTAATTTTATCCGCCCATTGGCTAAGCATTTGTAAGTCATGGCTGATAAGCAAAATAGTGGTATTGCTGTTTTGGTTGAGACGCGTca                                               <  2:62326/150‑1 (MQ=255)                                                                                                       ggCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACTGCAGTAAAGCACGTTAATTTTATCCGCCCATTGGCTAAGCATTTGTAAGTCATGGCTGATAAGCAAAATAGTGGTATTGCTGTTTTGGTTGAGACGCGTcagca                                            <  2:423605/151‑1 (MQ=255)                                                                                                           tCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACTGCAGTAAAGCACGTTAATTTTATCCGCCCATTGGCTAAGCATTTGTAAGTCATGGCTGATAAGCAAAATAGTGGTATTGCTGTTTTGGTTGAGACGCGTCAGCAgg                                          <  1:1043458/149‑1 (MQ=255)                                                                                                             gTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACTGCAGTAAAGCACGTTAATTTTATCCGCCCATTGGCTAAGCATTTGTAAGTCATGGCTGATAAGCAAAATAGTGGTATTGCTGTTTTGGTTGAGACGCGTCAGCAGGCGaa                                      <  2:1167850/151‑1 (MQ=255)                                                                                                                aCCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACTGCAGTAAAGCACGTTAATTTTATCCGCCCATTGGCTAAGCATTTGTAAGTCATGGCTGATAAGCAAAATAGTGGTATTGCTGTTTTGGTTGAGACGCGTCAGCAGGCGaa                                      >  1:691994/1‑148 (MQ=255)                                                                                                                       ccTTACTCGGCGCGGTTTCCACTGTTTGACTGCAGTAAAGCACGTTAATTTTATCCGCCCATTGGCTAAGCATTTGTAAGTCATGGCTGATAAGCAAAATAGTGGTATTGCTGTTTTGGTTGAGACGCGTCAGCAGGCGAAAGATTTgcg                             <  2:1041362/150‑1 (MQ=255)                                                                                                                       ccTTACTCGGCGCGGTTTCCACTGTTTGACTGCAGTAAAGCACGTTAATTTTATCCGCCCATTGGCTAAGCATTTGTAAGTCATGGCTGATAAGCAAAATAGTGGTATTGCTGTTTTGGTTGAGACGCGTCAGCAGGCGAAAGATTTgc                              <  2:1271836/149‑1 (MQ=255)                                                                                                                          tACTCGGCGCGGTTTCCACTGTTTGACTGCAGTAAAGCACGTTAATTTTATCCGCCCATTGGCTAAGCATTTGTAAGTCATGGCTGATAAGCAAAATAGTGGTATTGCTGTTTTGGTTGAGACGCGTCAGCAGGCGAAAGATTTGCGCCt                          <  1:264081/150‑1 (MQ=255)                                                                                                                          tACTCGGCGCGGTTTCCACTGTTTGACTGCAGTAAAGCACGTTAATTTTATCCGCCCATTGGCTAAGCATTTGTAAGTCATGGCTGATAAGCAAAATAGTGGTATTGCTGTTTTGGTTGAGACGCGTCAGCAGGCGAAAGATTTGCGCCt                          <  2:1028412/150‑1 (MQ=255)                                                                                                                           aCTCGGCGCGGTTTCCACTGTTTGACTGCAGTAAAGCACGTTAATTTTATCCGCCCATTGGCTAAGCATTTGTAAGTCATGGCTGATAAGCAAAATAGTGGTATTGCTGTTTTGGTTGAGACGCGTCAGCAGGCGAAAGATTTGCGCCTgg                        >  2:507931/1‑151 (MQ=255)                                                                                                                               ggCGCGGTTTCCACTGTTTGACTGCAGTAAAGCACGTTAATTTTATCCGCCCATTGGCTAAGCATTTGTAAGTCATGGCTGATAAGCAAAATAGTGGTATTGCTGTTTTGGTTGAGACGCGTCAGCAGGCGAAAGATTTGCGCCTGGgttg                    <  2:576388/151‑1 (MQ=255)                                                                                                                               ggCGCGGTTTCCACTGTTTGACTGCAGTAAAGCACGTTAATTTTATCCGCCCATTGGCTAAGCATTTGTAAGTCATGGCTGATAAGCAAAATAGTGGTATTGCTGTTTTGGTTGAGACGCGTCAGCAGGCGAAAGATTTGCGCCTGGgttg                    <  2:1186858/151‑1 (MQ=255)                                                                                                                                  gcgGTTTCCACTGTTTGACTGCAGTAAAGCACGTTAATTTTATCCGCCCATTGGCTAAGCATTTGTAAGTCATGGCTGATAAGCAAAATAGTGGTATTGCTGTTTTGGTTGAGACGCGTCAGCAGGCGAAAGATTTGCGCCTGGGttgttg                 <  1:367015/151‑1 (MQ=255)                                                                                                                                   cgGTTTCCACTGTTTGACTGCAGTAAAGCACGTTAATTTTATCCGCCCATTGGCTAAGCATTTGTAAGTCATGGCTGATAAGCAAAATAGTGGTATTGCTGTTTTGGTTAAGACGCGTCAGCAGGCGAAAGATTTGCGCCTGGGTTGTTgg                <  1:1156881/151‑1 (MQ=255)                                                                                                                                    ggTTTCCACTGTTTGACTGCAGTAAAGCACGTTAATTTTATCCGCCCATTGGCTAAGCATTTGTAAGTCATGGCTGATAAGCAAAATAGTGGTATTGCTGTTTTGGTTGAGACGCGTCAGCAGGCGAAAGATTTGCGCCTGGGTTGTTGGc               >  1:550208/1‑151 (MQ=255)                                                                                                                                       ttCCACTGTTTGACTGCAGTAAAGCACGTTAATTTTATCCGCCCATTGGCTAAGCATTTGTAAGTCATGGCTGATAAGCAAAATAGTGGTATTGCTGTTTTGGTTGAGACGCGTCAGCAGGCGAAAGATTTGCGCCTGGGTTGTTGGCTcc            >  2:912804/1‑151 (MQ=255)                                                                                                                                       ttCCACTGTTTGACTGCAGTAAAGCACGTTAATTTTATCCGCCCATTGGCTAAGCATTTGTAAGTCATGGCTGATAAGCAAAATAGTGGTATTGCTGTTTTGGTTGAGACGCGTCAGCAGGCGAAAGATTTGCGCCTGGGTTGTTGGCTcc            >  2:1281641/1‑151 (MQ=255)                                                                                                                                        tCCACTGTTTGACTGCAGTAAAGCACGTTAATTTTATCCGCCCATTGGCTAAGCATTTGTAAGTCATGGCTGATAAGCAAAATAGTGGTATTGCTGTTTTGGTTGAGACGCGTCAGCAGGCGAAAGATTTGCGCCTGGGTTGTTGGCTcc            >  1:987769/1‑150 (MQ=255)                                                                                                                                         ccACTGTTTGACTGCAGTAAAGCACGTTAATTTTATCCGCCCATTGGCTAAGCATTTGTAAGTCATGGCTGATAAGCAAAATAGTGGTATTGCTGTTTTGGTTGAGACGCGTCAGCAGGCGAAAGATTTGCGCCTGGGTTGTTGGCTCCAt          <  2:319360/151‑1 (MQ=255)                                                                                                                                              gTTTGACTGCAGTAAAGCACGTTAATTTTATCCGCCCATTGGCTAAGCATTTGTAAGTCATGGCTGATAAGCAAAATAGTCGTATTGCTGTTTTGGTTGAGACGCGTCAGCAGGCGAAAGATTTGCGCCTGGGTTGTTGGCTCCATTGAGt     <  1:1289144/151‑1 (MQ=255)                                                                                                                                                 tGACTGCAGTAAAGCACGTTAATTTTATCCGCCCATTGGCTAAGCATTTGTAAGTCATGGCTGATAAGCAAAATAGTGGTATTGCTGTTTTGGTTGAGACGCGTCAGCAGGCGAAAGATTTGCGCCTGGGTTGTTGGCTCCATTGAGTTgg  >  2:907182/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                                   AGTGGGATAGCGCCGGGCAGCGTATTGAGGCGACTTTTATGCGGCATCGCGCTGCCGAAGTCTGGTATCGCGCGGATCAGCGCCTGGGTATAAGGATGATGTGGCATCGTCACCAACTCCTTACTCGGCGCGGTTTCCACTGTTTGACCGCAGTAAAGCACGTTAATTTTATCCGCCCATTGGCTAAGCATTTGTAAGTCATGGCTGATAAGCAAAATAGTGGTATTGCTGTTTTGGTTGAGACGCGTCAGCAGGCGAAAGATTTGCGCCTGGGTTGTTGGCTCCATTGAGTTGG  >  NC_000913/1352778‑1353072

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑