Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,434,882 | 0 | T | G | 50.9% | 66.5 / 90.6 | 56 | Y252S (TAC→TCC) | truA | tRNA pseudouridine(38‑40) synthase |
Reads supporting (aligned to +/- strand): ref base T (12/15); new base G (28/0); total (41/15) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.46e-06 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.69e-09 |
TCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTT‑CCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCAT > NC_000913/2434737‑2435030 | tCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGTAGATCATACCGGTCAGGGGAATcc > 1:196392/1‑151 (MQ=255) gCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATACCGGTCAGGGGAATCCACCGCGAcc > 2:422242/1‑151 (MQ=255) gCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGAc < 1:230761/150‑1 (MQ=255) cTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATACCGGTCCGGGGAATCCACCGCGAcc > 2:485980/1‑149 (MQ=255) tATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATACCGGGCAGGGGAATCCACCGCGAcc > 1:88052/1‑148 (MQ=255) aTGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGAt < 1:861727/151‑1 (MQ=255) tGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGGAGATCATAACCGGCCGGGGAATCCCCCGCGGcc > 1:690904/1‑146 (MQ=255) tGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGAt < 1:663339/150‑1 (MQ=255) gCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATAc < 1:950693/151‑1 (MQ=255) gTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGAGCATAACTGGCAGGGGAATCCACCGCGACCCGATACAGccc > 1:751922/1‑151 (MQ=255) tCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGTCAGGGGAATCCACCGCGACCAGATACAGCCCTT‑cc > 1:603951/1‑151 (MQ=255) ttCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTT‑CCGCtttt < 1:132743/148‑1 (MQ=255) tCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTT‑CCGCTTTTGcc < 1:651429/150‑1 (MQ=255) aGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGGAATCCACCGCCACCAGATACAACCCTT‑TCGCTTTTTCCgct > 1:719906/1‑149 (MQ=255) gTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATTATACCGGGCAGGGGAAACCCCCGCGACCAGATAAAGCCCTT‑CCGCTTTTGTCGtt > 2:857487/1‑146 (MQ=255) tAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGTCAGGGGAATCCACCCCGCCCCGATACAACCCCT‑CCGCCTTTGCCGTtgctg > 2:618861/1‑149 (MQ=255) tAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCCGGCAGGGGAATCCACCGCCACCAGATACAGCCCTT‑CCCCTTTTTCCGTTgccgcc > 2:672083/1‑151 (MQ=255) aaCTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGGCAGGGGAATCCACCGCGACCAGATACAGCCCCT‑CCGCTTTTGCCGGTGCTgcc > 2:716822/1‑150 (MQ=255) aaCTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTT‑CCGCTTTTGCCGTTGCTgcc < 1:831405/150‑1 (MQ=255) aaaTGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGTCCGGGGAAATCACCGCGACCAGATAAAGCCCTT‑CCGCTTTTGCCGTTGCTGCCGCCAGCGATCTTTCCTTTgcc > 1:914526/1‑150 (MQ=255) ttCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGGAATCCACCGCGACCAGATACAGCCCTT‑CCGCTTTTGCCGTTGCTGCCCCCAGCGTTCTTTCCTTTTCCGCCAg > 1:567466/1‑150 (MQ=255) tCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGGCAGGGGAATCCACCGCGACCAGATACAGCCCTT‑CCGCTTTTGCCGTTGCTGCCGCCCGCGTTCTGTCCTTTTccgcccgc > 1:224207/1‑150 (MQ=255) attattCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATTATACCCGTCAGGGGAAACCACCCCGACCCGATACAGCCCCT‑CCGCTTTTGCCGTTGCTGCCCCCCGCGGTCTGTCCTTTGCCCCCCGCAACTCTg > 2:299212/1‑150 (MQ=255) attattCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGCAGATCATACCCGTTAAGGGGAACCACCGCGCCCAGATCCACGCCCT‑CCGCTTTTTCCAGTGTTGGCGCCACCGCGCTGTCCTTTTCCGCCAGCGCCTCTGc > 1:807280/1‑151 (MQ=255) ttattCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGAACATACCGGTTAGGGTAAACCCCCACGACCAAAAACAGCCCTT‑CCGCTTTTGCCGTTGCTGCCCCCCTCCTTCTGTCCTTTtccgcccccaa > 1:456178/1‑139 (MQ=255) ttattCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGGAGATCATACCGGTCAGGGGAATCCACCGCGACCAGATACAGCCCTT‑CCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACCCTGc > 1:261126/1‑150 (MQ=255) ctcGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATAACCGTCAGGGGAAATCACCGCGACCAGATACAAGCCTT‑CCCCTTTTGCCGTTGCTGCCCCCAGCGGTCTGTCCCTTTCCCCCCGCAACTCTGCTCTcc > 2:322921/1‑150 (MQ=255) tcGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATACCGGGCAGGGGAATCCACCGCGGCCAGATACAGCCCTT‑CCCCTTTTTCCGTTGCTGCCGCCAGCGTTCTGTCCTTTTGCGCCCGCAACTCTGCt > 2:474871/1‑145 (MQ=255) tcGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAAATCATACCCGGCAGGGGAATTCACCCCGACCCGATACAGCCCCT‑CCGCTTTTTCCGTTGCTCCCCCCAGCGCTCTGTCCTTTTCCCCCAACCACtcttcttttc > 2:707252/1‑145 (MQ=255) tcGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGGAAATAATACCCGGCCGGGGAATCCACCGCGGCCAGATACAGACCTT‑CCCCTTTTTCCGCTGCTGGCCCCAGCGGTTTGTCCTTTGCCGCCACCAACTCTGCTATcc > 1:867310/1‑149 (MQ=255) gTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGGAGATCATACCCGGCAGGGGAATCCACCGCGGCCAGATACAGCCCCT‑TCGCCTTTTCCGTTGCTGCCCCCCGCCGTCTGTCCTTTTCCGCCAGCAACCCTGCCATcc > 2:593574/1‑147 (MQ=255) aGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATAACGGTCAGGGGAATCCACCGCGACCAGATACAGCCCTT‑CCGCTTTTTCCGTTGCTGCCGCCCGCGTTCTGTCCTTTGCCGCCAGCAACCCTGCTATCCAGCcctc > 1:562139/1‑151 (MQ=255) tCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGAAGCTTCCGGTTAGGGTAAGCCACCCCCGCCGGATAAAGGCCCT‑CGGCTTTTCCCCCTGCCCCCGCCCGCGGTCTGTCCCTTGCCGCCAGGCACACAGCCGTCCACCTCTcc > 2:116074/1‑150 (MQ=255) gAAATAGCGGGCCCATTGGCGGTTTTGGGAAGTTATCCCGGGGCGGGGAAAACACCCCGGCCAGCAACAACCCCT‑CCCCCTTTGCCGTTGCTGCCGCCCACGGTTCCTGCTTTGACGCCCGCCAAACCGCTATCCCGCTCGCCGGCTggg > 2:665129/1‑149 (MQ=255) aTAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGGCAGGGCAATCCACCGCGACCAGATAAAACCCTTCCCCCTTTTCCCGTT‑CTGCCGCCAGCGGTCTGGCCTTTTCCCCCAACAACCCTTCTATTCAGCCCTCCTGCCgggtttg > 2:790943/1‑146 (MQ=255) aTAGCGGGCCCATTGGCGGTTTTGGAAGATCATAACGGGCAGGGGAAACCACCGCGACCAGATAAAGCCCTT‑CCGCTTTTTCCGTTGCTGCCGCCAGCGGTCTGTCCTTTGCCGCCAGCAACTCTGCTATTCAGCTCTCCGGCTgggt > 1:619634/1‑146 (MQ=255) cGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTT‑CCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCa < 2:631115/151‑1 (MQ=255) gggCCCATTGGCGGTTTTTGGAGATCATACCGGTCAGGGGAATCCACCCCGACCCGATACAGCCCTT‑CCCCTTTTGCCGTTGCTGCCCCCCGCGGTCTGTCCTTTGCCGCCAGCAACTCTTCTATCCCGCTCTCCGGCTGGGTGTGGGCAc > 2:378267/1‑151 (MQ=255) gggCCCATTGGCGGTTTTGGGAGATCATACCGGGCAGGGGAATCCACCGCGACCAGATACAGCCCTT‑CCCCTTTTGCCGTTGCTGCCGCCCGCGTTCTGTCCCTTTCCGCCCGCAACCCTTCTTTCCCGCTCTCCCGCCGGGTGTgggga > 2:744815/1‑148 (MQ=255) ggCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTT‑CCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTcc > 1:673002/1‑150 (MQ=255) tttGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTT‑CCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAgg < 1:64108/150‑1 (MQ=255) ttGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTT‑CCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCt > 1:626696/1‑151 (MQ=255) tGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTT‑CCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCt < 1:367449/150‑1 (MQ=255) gAAGATCATACCGGTCGGGGTAATCCCCCGCGACCAGATACAGCCCTT‑CCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTg < 2:153352/149‑1 (MQ=255) gAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTT‑CCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGcc > 1:146369/1‑151 (MQ=255) aGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTT‑CCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCg > 2:452993/1‑150 (MQ=255) aTACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTT‑CCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAat > 1:543803/1‑150 (MQ=255) aCCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTT‑CCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAatat < 1:726165/150‑1 (MQ=255) aCCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTT‑CCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATAtt < 2:786315/151‑1 (MQ=255) gTCAGGGTAATCCACCGCGACCAGATACAGCCCTT‑CCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTg < 1:708237/151‑1 (MQ=255) aGGGTAATCCACCGCGACCAGATACAGCCCTT‑CCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGAcc > 2:571984/1‑151 (MQ=255) aGGGTAATCCACCGCGACCAGATACAGCCCTT‑CCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGAcc > 2:565690/1‑151 (MQ=255) aGGGTAATCCACCGCGACCAGATACAGCCCTT‑CCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGAcc > 1:211994/1‑151 (MQ=255) aGGGTAATCCACCGCGACCAGATACAGCCCTT‑CCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGAcc > 2:844074/1‑151 (MQ=255) ggTAATCCACCGCGACCAGATACAGCCCTT‑CCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCat > 2:641528/1‑151 (MQ=255) ggTAATCCACCGCGACCAGATACAGCCCTT‑CCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCat < 1:95989/151‑1 (MQ=255) | TCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACAGCCCTT‑CCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACCAT > NC_000913/2434737‑2435030 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |