Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,222,596	0	G	A	46.0%	21.7 / 26.7	37	F13F (TTC→TTT)	ymgD	uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (16/4);  new base A (0/17);  total (16/21)
Fisher's exact test for biased strand distribution p-value = 5.89e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAACGCTGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTT‑AATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAACGCCAGTGAC  >  NC_000913/1222448‑1222739                                                                                                                                                      |                                                                                                                                                cAACGCTGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTT‑AATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGaa                                                                                                                                               <  2:846843/151‑1 (MQ=255)         gCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTT‑AATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAaacaaaca                                                                                                                                       <  2:398960/151‑1 (MQ=255)                  attaccGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTT‑AATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAGGCCCGcc                                                                                                                               >  2:73152/1‑150 (MQ=255)                   ttaccGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTT‑AATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAGGCCCGcc                                                                                                                               >  2:281578/1‑149 (MQ=255)                       cGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTT‑AATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAGGCCCGCCAGTaa                                                                                                                          >  1:761359/1‑150 (MQ=255)                             aaTTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTT‑AATATTGTAGTCCTGCGCCCAGGACATTGGTGCGAAAACAAACAGGCCAGCCAGTAAAGCAAAt                                                                                                                   >  2:639754/1‑151 (MQ=255)                               ttccTGCACCCTGTGCGCGGTCATTTTTTCCCTTTTCGTTGGCTTCGGCACAAGTAATATAGTTTTCGGACGGCAAACCTTTTTT‑AATTTTGTAGTCCGGCCCCCAGTTCATTGGGGCAAAAACAAACAGGCCCGCCAGTAAAGCAAAtt                                                                                                                  <  1:196038/147‑1 (MQ=255)                                        ccTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCAAAAGTAATATAGTTTTCTGACGGCAACCCTTTTTT‑AATATTGTAGCCCTGCCCCCAGGTCATGGGTGCAAAAAAAAACAGGCCCGCCAGTAAAGCAAATTTTTtcatcat                                                                                                        <  1:744866/151‑1 (MQ=255)                                                 tGTCAGTTTTTCCCTTTTCGTTGGTTTCTGCGAAAGTAATATGGGTTTCTG‑CCGCAAACCCTTTTTAAATATTGTGGCCCTGCGCCCAGGTCATTGGTGCGAAAAAAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCtt                                                                                                <  1:142205/150‑1 (MQ=255)                                                      ttttttGCCTTTTCGTTGGTTTCTGCGCAAGTAATATAGTTTTCTGACGGCAAACCTTTTTT‑AATTTTGTAGCCCTGCCCCCAGTTCTTTGGTGCAAAAAAAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTAtt                                                                                             <  2:367596/147‑1 (MQ=255)                                                       tttttGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTT‑AATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCAt                                                                                         >  2:611826/1‑151 (MQ=255)                                                            gCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTT‑AATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTAc                                                                                    >  2:34582/1‑151 (MQ=255)                                                             ccttttCGTTGCTTTCTGCGAAAGAAATATAGGTTTCGGACGGCAAACCGTTTTT‑AATTTAGTAGTCCTGGGCCCAGCTCATTGGTCAAAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCAtttt                                                                                      <  1:916992/145‑1 (MQ=255)                                                                tttCGTTGGTTTCTGCGCAAGTAATATAGGTTTCTGACGGAAACCCTTTTTT‑AATATTGTAGCCCTGCGCCCAGTTCTTTGGTGCAAAAAAAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCa                                                                                 <  2:341930/150‑1 (MQ=255)                                                                  ttgTTGTCTTCTGCTAAAATAAAAGCGGTTTCTGACGCAAAACCTTTTTT‑AATTTTGTTGTCCTTCCCCAAGTTTTTTGCTGCAAAAACAAACATGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGa                                                                               <  2:536172/148‑1 (MQ=255)                                                                    gTTGGTTTCGGCGCAATTAATATAGGTTTCTGCCGGAAACCCTTTTTT‑AATATTGTAGCCCGGCCCCCAGGTCATTGGTGAAAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGaa                                                                              <  1:846553/149‑1 (MQ=255)                                                                     ttGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTT‑AATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAAtt                                                                            >  2:797439/1‑150 (MQ=255)                                                                     ttGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTT‑AATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTg                                                                           >  2:4773/1‑151 (MQ=255)                                                                     ttGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTT‑AATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTg                                                                           >  1:790814/1‑151 (MQ=255)                                                                          ttCGGCGCAAGAAATATAGTTTTCTGCCGACAAACCTTTTTA‑AATATTTTATTCCTGCCCCCAGTTCTTTGGTGCAAAAAAAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGca                                                                         <  2:312105/148‑1 (MQ=255)                                                                           tCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTT‑AATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGcaccac                                                                     >  2:846902/1‑151 (MQ=255)                                                                              gcgcAAGTAATATAGTTTTCTGACGGAAACCTTTTTTT‑AATATTGTAGCCCTGCCCCCAGGTCATTGGTGCAAAAACAAACAGCCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGcaccac                                                                     <  2:162217/148‑1 (MQ=255)                                                                                  aaGTAATATAGGTTTCTGACGGCAACCCTTTTTA‑AATATTGTAGCCCTCCCCCCAGTTCTTGGTTGCAAAAACAAACAGCCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCAccc                                                                   <  2:452066/146‑1 (MQ=255)                                                                                  aaGTAATAGTGTTTTCTGACGGAAAACCTTTTTA‑AATATGGTAGTCCTGCGCCCAGGTTTTTGGTGCAAAAAAAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCg                                                                  <  2:878784/147‑1 (MQ=255)                                                                                      aaTATAGTTTTTTGCCGGAAAACTTTTTTT‑AATTTTGTAGTCCTGCGCCCAGGTAATTGGTGCAAAAACAAACAGCCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCg                                                             <  2:646471/148‑1 (MQ=255)                                                                                            ggTTTATGACGGAAACCCTTTTTA‑AATTTTGTATTCCGGCCCCCAGTTCTTGGGTGCAAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATc                                                       <  2:44682/148‑1 (MQ=255)                                                                                                     cGGCAAACCGTTTTT‑AATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCttt                                            >  2:308693/1‑150 (MQ=255)                                                                                                         aaccttttttt‑AATATTGTAGCCCTGCCCCCAGGTCATTGGTGCAAAAAAAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCttt                                            <  1:116497/140‑1 (MQ=255)                                                                                                          accctttttt‑AATTTTAAACCCCTGCCCCGAGTTCTTTGATAAGAAAACAAACCGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTaa                                          <  2:304927/142‑1 (MQ=255)                                                                                                           aCCGTTTTT‑ACTATTGTAGTCCTGCGCCCAGGTCATTGGTGCGACAACAAACAGGCAGGGCCGTAGAGCAAATTTTTTCATCAGCATTTCTTATTTCATTTTACCCAGAATAGCACCACCCGTACCGCCAATCACGACAcc                                               >  2:336573/1‑141 (MQ=255)                                                                                                            ccGTTTTT‑AATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGccc                                    >  2:23929/1‑151 (MQ=255)                                                                                                                       ttttttgttCCTGCCCCCGGGTTGTTGGTGAAAAAAAAACGAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAgg                            <  1:655720/141‑1 (MQ=255)                                                                                                                                ccggccccaagttttttGGTGAAAAAACAAACGGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCgg                  <  2:6537/135‑1 (MQ=255)                                                                                                                                       ccAGGTCATTGGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAACg          >  1:294191/1‑151 (MQ=255)                                                                                                                                            tCATTGGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAACGCCAGt     >  1:26524/1‑151 (MQ=255)                                                                                                                                               tgggTGCAAAAACAAACGGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAACGCCAGTg    <  1:12591/147‑1 (MQ=255)                                                                                                                                                tGGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAACGCCAGTGAc  >  1:570500/1‑150 (MQ=255)                                                                                                                                                      |                                                                                                                                                CAACGCTGGCATTACCCATTACCGCAACAATTTCTGCAACCTGTGCGCTGTCAGTTTTTGCCATTTCGTTGGCTTCTGCGCAAGTAATATAGGTTTCTGACGGCAAACCGTTTTT‑AATATTGTAGTCCTGCGCCCAGGTCATTGGTGCGAAAACAAACAGGCCCGCCAGTAAAGCAAATTTTTTCATCATCATTCCTTATTTCATTTTACCCAGAATTGCACCACCCGTACCGCCAATCACGGCACCTTTAATCGCCCCTTCGAGGCCATTGCCGGTCAGAACGCCAGTGAC  >  NC_000913/1222448‑1222739

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑