| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,578,301 | 0 | A | C | 43.6% | 85.7 / 14.9 | 55 | G142G (GGT→GGG) | gntR | d‑gluconate inducible gluconate regulon transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base A (25/6); new base C (0/24); total (25/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.42e-10 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGTCGAGACGTGCGCCGAGATAGGCAATGTGGCGATGCCCGCGAGCAATAATGGCAGTGG‑TCATCTGGCGTG‑CTGCTTCAAAGTTATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCC‑ATCAGTT‑CCACCA‑CGGG‑AATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTTATAACCGTAGTGCGCCAGCATGGTCTGATA > NC_000913/3578153‑3578447 | cgtcgAGACGTGCGCCGAGATAGGCAATGTGGCGATGCCCGCGAGCAATAATGGCAGTGG‑TCATCTGGCGTG‑CTGCTTCAAAGTTATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCC‑ATCAGTT‑CCACCA‑CGGG‑AATAcc < 2:1032336/151‑1 (MQ=255) gtcgAGACGTGCGCCGAGATAGGCAATGTGGCGATGCCCGCGAGCAATAATGGCAGTGG‑TCATCTGGCGTG‑CTGCTTCAAAGTTATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCC‑ATCAGTT‑CCACCA‑CGGG‑AATAccc > 1:4528/1‑151 (MQ=255) gtcgAGACGTGCGCCGAGATAGGCAATGTGGCGATGCCCGCGAGCAATAATGGCAGTGG‑TCATCTGGCGTG‑CTGCTTCAAAGTTATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCC‑ATCAGTT‑CCACCA‑CGGG‑AATAccc < 2:1114903/151‑1 (MQ=255) gagaCGTGCGCCGAGATAGGCAATGTGGCGATGCCCGCGAGCAATAATGGCAGTGG‑TCATCTGGCGTG‑CTGCTTCAAAGTTATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCC‑ATCAGTT‑CCACCA‑CGGG‑AATACCCGcc > 2:149913/1‑151 (MQ=255) gaCGTGCGCCGAGATAGGCAATGTGGCGATGCCCGCGAGCAATAATGGCAGTGG‑TCATCTGGCGTG‑CTGCTTCAAAGTTATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCC‑ATCAGTT‑CCACCA‑CGGG‑AATACCCGCCa < 1:633439/150‑1 (MQ=255) ggaattggggggATGCCCGCGAGAAATAAGGGCGGTGT‑TCATCTGGCGTC‑GTGTTTAAAATTTATAAAAC‑CCGACGGCGATATCAGGGCAGGGCGCCTTGCTGTCC‑ATCAGTC‑CCACCA‑GGGA‑AATCCCCGCCACTTCAATCATCttt < 1:559619/137‑1 (MQ=255) ggCAATGTGGCGATGCCCGCGAGCAATAATGGCAGTGG‑TCATCTGGCGTG‑CTGCTTCAAAGTTATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCC‑ATCAGTT‑CCACCA‑CGGG‑AATACCCGCCACTTCAATCATCTTTa > 1:485358/1‑149 (MQ=255) gCAATGTGGCGATGCCCGCGAGCAAT‑ATGGCAGTGG‑TCATCTGGCGTG‑CTGCTTCAAAGTTATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCC‑ATCAGTT‑CCACCA‑CGGG‑AATACCCGCCACTTCAATCATCTTTAAGGt > 1:384749/1‑151 (MQ=255) aaagggggggATCCCCGGGAAAAATAAGGGCGGTGG‑TCTTGGGGGGGG‑CGGCTACAAATTTATAAAAC‑CCGACGGCGATATCAAGGCATGGCTATTTGTTGTCC‑ATCGGTT‑CCCCCA‑CGGA‑AATCCCCGCCACTTCAATCATCTTTAAGGt < 2:1057720/142‑1 (MQ=255) aaTGGGGCGATGCCCGCAAAAAAAAAGGGCGGTGG‑TCACCGGGCGTG‑CTGCTCAAAATTTAAAAAAC‑CCGCCGGCAATAAAAAGGCAGGGCTCTTTGCTGCCC‑ATCAGTC‑CCCCCC‑GGGA‑AATCCCCGCCACTTCAATCATCTTTAAgg < 2:469651/149‑1 (MQ=255) gggggCGATGCCGGCGAGAAATAGTGGAGGTGG‑TCATC‑GGGGTGCCTGCTAAAAAGTTACAAAAC‑CCGACGGCGATATCAGGCCGTGGCGTCTTGCTGTCC‑ATCATTC‑CCCCCA‑GGGA‑AACCCCCGCCACTTCAATCATCTTTAAGGTgc < 2:404166/147‑1 (MQ=255) ggggATGCCCGGGAGAAATAAGGGCGGTGG‑TCATCTGGCGTT‑TTGCTCAAAAGTTAAAAAAC‑CCGACGGCGATATCAAGGCAGGGCGCCTTCCTGTCC‑ACCAGTC‑CCCCCA‑CGGA‑AATACCCGCCACTTCAATCATCTTTAAGGTgc < 1:756390/144‑1 (MQ=255) aGCAATAATGGCAGTGG‑TCATCTGGCGTG‑CTGCTTCAAAGTTATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCC‑ATCAGTT‑CCACCA‑CGGG‑AATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGt > 1:253293/1‑151 (MQ=255) ataatGGCAGTGG‑TCATCTGGCGTG‑CTGCTTCAAAGTTATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCC‑ATCAGTT‑CCACCA‑CGGG‑AATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCgg > 1:451009/1‑151 (MQ=255) aatGGCGGTGG‑TCTTCGGGCGTC‑CT‑CTTTAAAATTAACAAAACCCGCCGGCAATACAAAGGCAGGGCTCTTTGCTGCCC‑ATCAGTC‑CCCCCA‑CGGA‑AATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCgg < 2:708115/149‑1 (MQ=255) atGGCAGTGG‑TCATCTGGCGTG‑CTGCTTCAAAGTTATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCC‑ATCAGTT‑CCACCA‑CGGG‑AATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTg > 1:148000/1‑150 (MQ=255) agggCGGTGG‑TCATCTGGTTTG‑CTTATTTAAGGTAACAAACG‑CCCGCGGCGATAACAAGGCAGGGCGTCTTGCTGTCC‑ACCAGTT‑CCCCCA‑GGGA‑AATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGt < 1:726612/147‑1 (MQ=255) ggaGTGGTTCATCTGGGGTG‑CTG‑TTCAAAATTTACAAAACCCGCCGGCGATATAAAGGCGTGGCGCTTTGCTGTCC‑ATCAGTC‑CCCCCA‑GGGG‑AATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAg < 2:1007465/148‑1 (MQ=255) gTGG‑TCTTCGGGCGTG‑CTCCTCAAAATTTAACAAAC‑CCGACGAGAATACCAGGGCATGGCGTCTTGTTCCCC‑ATCATTC‑CCCCCA‑GGGA‑AATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCa < 2:607016/151‑1 (MQ=255) gttcttCTGGCGTG‑CTGCTTAAAATTTATAAAAC‑CCGCCGGCAATATAAAGGCATGGCGCCTTGTTGCCC‑ACCAGTC‑CCCCCA‑GGGA‑AATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAg < 1:653334/145‑1 (MQ=255) ggtcttggggCGTG‑CTGTTTAAAAGTTATAAAAC‑CCGAGGGCAATACCAAGGCAGGGCGACTTGCTGCCC‑ACCAGTC‑CCCCCG‑CGGA‑AATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATc < 1:429843/140‑1 (MQ=255) tcgggggTG‑CTGTTTAAAATTTAAAAAAC‑CCGGGGGCGATAACAAGGCAGGGCGATTTGCTTCCC‑ATCAGTC‑CCCCCG‑GGGA‑AATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGt < 1:464592/144‑1 (MQ=255) ggggTG‑CTGCTCAAATTTTAAAAAAC‑CCGGCGGCGAAACAAGGGCAGGGCGTTTTCCTGTCC‑ACCAGTC‑CCCCCG‑GGGG‑AATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCg < 1:13839/143‑1 (MQ=255) ggCGTG‑CTTCTAAAAATTTACAAACC‑CCGAGGGCAATATCAGGGCATGGCGTCTTGCTGTCC‑ATCA‑TT‑CCCCCC‑CGGGAAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATc < 1:742458/140‑1 (MQ=255) ggCGTG‑CTGCTTAAAATTAAAAAAAC‑CCGCCGGCGATAACAAGGCATGGCGCTTTTCTGTCC‑ATCGGTC‑CCCCCG‑GGGA‑AATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTc < 1:251141/148‑1 (MQ=255) gTG‑CTGCTTCAAAGTTATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCC‑ATCAGTT‑CCACCA‑CGGG‑AATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATAtt > 1:424612/1‑151 (MQ=255) g‑ctgctTCAAAGTTATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCC‑ATCAGTT‑CCACCA‑CGGG‑AATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTcc > 1:652089/1‑151 (MQ=255) tgctaaaaaTTTACCAAAC‑CCGACGGCGATATCAAGGCAGGGCGTCTTGCTGTCC‑ATCAGTC‑CCCCCA‑CGGG‑AATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTcc < 1:277430/143‑1 (MQ=255) gcataaaattaaacAAAC‑CCGAAGGCGAAATCAGGGCAGGGCGGTTTGCTCACC‑‑TTAGTTCCCCCCA‑CGGA‑AAGCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCa < 2:1109013/136‑1 (MQ=255) ttCAAAGTTATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCC‑ATCAGTT‑CCACCA‑CGGG‑AATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAgg > 1:574857/1‑149 (MQ=255) cAAAGTTATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCC‑ATCAGTT‑CCACCA‑CGGG‑AATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGgag > 2:1080674/1‑149 (MQ=255) aaatttaaaaaac‑CCGAGGGCAATATCAAGGCTGGGCGCCTGGCTGTCC‑ACCA‑TT‑CCCCCC‑CGGGAAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGga < 1:398417/138‑1 (MQ=255) aaatttaaaaaac‑CCGACGGCGATATCAGGGCAGGGCGTTTTGCTGTCC‑ATCAGTC‑CCACCA‑GGGA‑AATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTc < 1:50606/133‑1 (MQ=255) tATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCC‑ATCAGTT‑CCACCA‑CGGG‑AATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATgg > 1:443155/1‑150 (MQ=255) aacAAAA‑CCGCCGGCAATATCAAGGCAGGGCGATTTGC‑GTCCTATCAGTC‑CCGCGA‑GGGG‑AATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATgg < 2:630797/147‑1 (MQ=255) gACGGCGATATCAAGGCATGGCGACTTGCTGTCC‑ATCAGTT‑CCACCA‑CGGG‑AATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGt > 2:809026/1‑151 (MQ=255) ggCGATATCAAGGCATGGCGACTTGCTGTCT‑ATCAGTT‑CCACCA‑CGGG‑AATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCt > 2:570392/1‑151 (MQ=255) ggCGATATCAAGGCATGGCGACTTGCTGCCC‑ATCAGTC‑CCCCCG‑CGGG‑AATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCt < 1:993670/151‑1 (MQ=255) gCGATATCAAGGCATGGCGACTTGCTGTCC‑ATCAGTT‑CCACCA‑CGGG‑AATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCtt > 2:159523/1‑151 (MQ=255) gATATCAAGGCATGGCGACTTGCTGTCC‑ATCAGTT‑CCACCA‑CGGG‑AATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGc > 2:100875/1‑151 (MQ=255) tCAAGGCATGGCGACTTGCTGTCC‑ATCAGTT‑CCACCA‑CGGG‑AATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTcc > 2:122503/1‑150 (MQ=255) cAAGGCATGGCGACTTGCTGTCC‑ATCAGTT‑CCACCA‑CGGG‑AATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCAt > 1:17959/1‑151 (MQ=255) aaGGCATGGCGACTTGCTGTCC‑ATCAGTT‑CCACCA‑CGGG‑AATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCAtt > 1:323218/1‑151 (MQ=255) ggcggggCGTCTTGCTGCCC‑ATCAGTT‑CCCCCA‑GGGG‑AATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCAttt < 1:548127/145‑1 (MQ=255) ggCGTCTTCCTCTCC‑ATCATTC‑CCCCCA‑GGGA‑AATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGt < 2:452712/150‑1 (MQ=255) ggCGACTTGCTGTCC‑ATCAGTT‑CCACCA‑CGGG‑AATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGtt > 2:353421/1‑151 (MQ=255) gcccttctctcccc‑ATCA‑TT‑CCCCCATCGGA‑AATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGt < 1:771552/137‑1 (MQ=255) ccttttGCTGCCC‑ATCAGTC‑CCCCCC‑CGGA‑AATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGttt < 1:920442/146‑1 (MQ=255) gTCC‑ATCAGTT‑CCACCA‑CGGG‑AATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTTATAACCGt > 2:1131524/1‑149 (MQ=255) ccacca‑CGGG‑AATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTTATAACCGTAGTGCGCCAGCAt > 1:64793/1‑151 (MQ=255) ccccggggg‑AACCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTTATAACCGTAGTGCGCCa < 2:4528/140‑1 (MQ=255) cGGG‑AATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTTATAACCGTAGTGCGCCAGCATGGTCTg > 1:1133185/1‑151 (MQ=255) cGGG‑AATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTTATAACCGTAGTGCGCCAGCATGGTCTg > 1:1089166/1‑151 (MQ=255) aaaTCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTTATAACCGTAGTGCGCCAGCATGGTCTGAt < 1:700068/149‑1 (MQ=255) aaTACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTTATAACCGTAGTGCGCCAGCATGGTCTGATa > 1:344591/1‑150 (MQ=255) | CGTCGAGACGTGCGCCGAGATAGGCAATGTGGCGATGCCCGCGAGCAATAATGGCAGTGG‑TCATCTGGCGTG‑CTGCTTCAAAGTTATCAAAA‑CCGACGGCGATATCAAGGCATGGCGACTTGCTGTCC‑ATCAGTT‑CCACCA‑CGGG‑AATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTTATAACCGTAGTGCGCCAGCATGGTCTGATA > NC_000913/3578153‑3578447 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |