Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 357,880 | 0 | T | C | 45.5% | 54.3 / 13.0 | 44 | K271E (AAA→GAA) | cynR | transcriptional activator of cyn operon; autorepressor |
Reads supporting (aligned to +/- strand): ref base T (22/2); new base C (0/20); total (22/22) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 2.62e-10 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCA‑TGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGT‑CATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTG > NC_000913/357733‑358023 | cGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCA‑TGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTc < 2:224275/149‑1 (MQ=255) aGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCTCCAACAACCGCGCATTTATCCAACGCCA‑TGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTccgcc > 2:1092402/1‑150 (MQ=255) ccTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCA‑TGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGcaa < 1:251860/151‑1 (MQ=255) gATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCA‑TGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGcaaca > 2:554344/1‑150 (MQ=255) aaGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCA‑TGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAAc > 2:996983/1‑151 (MQ=255) acattcccAGCCGCAGACTTGTTTCAGGGGCCCCCGTGATTCATTCCCGCAAAAAACCGCCCTTTTACCCAACCCCA‑GGTCAAAAAATCCCTCCGCGGCGGCTGTCTGCCAGTTTTTTTCCCGCCGCAACAAAACCGCCGTtctct < 2:1083644/140‑1 (MQ=255) cAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCA‑TGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCagt > 2:138569/1‑150 (MQ=255) aaTTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCA‑TGTGC‑AAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCagtagt > 2:663064/1‑151 (MQ=255) ccAGCCTCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCA‑TGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTg > 2:109120/1‑149 (MQ=255) ccAGCCGCGGCCTTGTGTCAGGGGCTCCCTTGTTTCATTTCCCCCAACACCCGCGCTTTTACCCACCGCCA‑TGTAAAAAAATCCTTTCGCGGCGGCTGTCTGCCAGCTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGc < 1:134995/151‑1 (MQ=255) aGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCA‑TGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCgg > 2:839109/1‑151 (MQ=255) aGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCA‑TGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCgg > 1:901648/1‑151 (MQ=255) aGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCA‑TGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCgg > 2:256701/1‑151 (MQ=255) ccGCAGCCTTGTGCCAGGGCCTCCCGTGATTCTTTCCCCCCAACACCCGCGCATTTACCCAACCCCA‑TGTGAAAAAATGCCTTCGCGGCGGCTGTCTCCCACTTTTTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCgggg < 1:9772/151‑1 (MQ=255) cGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCA‑TGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCgggg > 1:793438/1‑150 (MQ=255) gCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCA‑TGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCggggt > 1:386719/1‑149 (MQ=255) gCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCA‑TGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCgg > 2:488940/1‑147 (MQ=255) gcccTTTGTCGGCGGCTACCGTGTTTCATCCCCGCCAACACCCGCGCATTTACCCAACGCCA‑TGTGAAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAag < 1:929375/148‑1 (MQ=255) cTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCACGCATTTATCCAACGCCA‑TGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCggggggcg > 1:62353/1‑143 (MQ=255) gtccccggtgtTTCATTCCCGCCACCACCCGCG‑TTTTAACCCACCCCATTGGGAAAAAATGCCTTCGGGGGGGCTGTCGCCAAGTTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAgaga < 2:759033/133‑1 (MQ=255) caaCCTTGCTTCATTCCCGCCAACACCCGCGCTTTTAACCAACGCCT‑TGGACAAAAATTCCCCCGCGCCGTCTGTCTGCCATCTTTTTTCCCGCCAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTaa < 2:70570/147‑1 (MQ=255) cTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCA‑TGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGGAGGGAAAAAgatgtt > 2:407911/1‑143 (MQ=25) cgttaaTTCTTTCCCCCAAACACCCGCGTTTTAACCCACCCCCA‑TGTGAAAAAATGCCTCCGGGGCGCCTGTCTGCCTGTTATTTTCCCCCCCAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCt < 2:675674/137‑1 (MQ=255) ccGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCA‑TGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGGGGAGAAATaacatttaaac > 2:75430/1‑139 (MQ=25) ccGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCA‑TGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGAAAAGAAAAAACtttttatcc > 1:956624/1‑144 (MQ=25) tGATTCATCCCCCCCAACACCCGCGCTTTTACCCAACGCCT‑TGTAAAAAAATGCTTCCGCGGGGGCTGTCTCCCAGCTATTTTCCCGCCAAAAAAAACCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCg < 2:1070312/148‑1 (MQ=255) accacccGCCCATTTACCAACCGCCT‑GGTAAAAAATGGCCTGCGCGGCGGCTGTCGGCCATTTATTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGT‑CATGTTGTGTGGCAAt < 1:506855/145‑1 (MQ=255) aacaacCGCGCATTTATCCAACGCCA‑TGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGGCGAGAAAAAAAATTTAACCCCC‑CCTGTTGTGTgggg > 2:609613/1‑146 (MQ=21) acaccCGCCCATTCACCCAACCCCT‑GGTAAAAAAATCCCTTCGCGGCGGTTGTCTGCCAGCTTTTTTCCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGT‑CATGTTGTGTGGCa < 1:612365/147‑1 (MQ=255) gcattcccccAACTCCA‑TGAACAAAAAGGCCTCCGCGGCGCCTTCCTCCCAGCTTTTTTCCCGCCGCAAAAAACCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGT‑CATGTTGTGTGGCAATCGCTGCTgg < 2:124077/142‑1 (MQ=255) tttccccacccccA‑TGAGAAAAAATCCCTCCGGGGCGGCTTTCGCCCAGTTTTTTTCCCGCCAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGT‑CATGTTGTGTGGCAATCGCTGCTGGTa < 2:486361/138‑1 (MQ=255) tttACCCAACGCCT‑TGTGAAAAAATGCTTCCGCGGCGGCTGTCTCCCAGTTTTTTTCCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGT‑CATGTTGTGTGGCAATCGCTGCTGGTa < 1:627872/149‑1 (MQ=255) tCCAACGCCA‑TGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGGAGAGAAAAAACTTTTAAACCCT‑CCTGTTGTGTGGGGATTGCTGCTGGtgaaaa > 1:423473/1‑144 (MQ=25) accgccagtggaaaaaaTCCCTTCGGGGCGGCTGTCGCCCATTTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGT‑CATGTTGTGTGGCAATCGCTGCTGGTAACaa < 2:133230/134‑1 (MQ=255) tgtgCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGCAGAGAAAAAAATTTTAACCCCC‑CCTGTTTTGTGGGAAACGCCGCTGGGGAAAAAGTGGAAAggg > 1:70883/1‑150 (MQ=255) gtgAAAAATTCCCTTCGCGGCGGCTGTCTGCCAGTTTTTTTCCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGT‑CATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAgg < 1:937868/148‑1 (MQ=255) cAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGAAGAGAAAAAAATTTTAACCCCC‑CCTGTTGTGTGGGGATCGCGTCTGGGAAAAAAGTGGGAAGGGGAggg > 1:818723/1‑149 (MQ=255) aaaagtccattCGCGGCGGCTGTCTCCGCGTTTTTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGT‑CATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAg < 2:48734/139‑1 (MQ=255) aaatTGCCTCCGGGGCGGCTGTCCCCCATTTTTTTTCCCCCCACAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGT‑CATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTgcg < 1:442887/151‑1 (MQ=255) tGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGGCGAGAAAAAAATTTTAAAACCC‑CCTCTTGTGGGGGAAACGCTGGTGGGGAAAATGTGGGAAGGGGAGGggggcg > 2:213372/1‑150 (MQ=255) cTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCGAGGGCAAAAAATTTAAATCCTTCCACTTTGTG‑GGGGATTGCTGCTGGGAACATTATGGAAAaggggagag > 2:813350/1‑137 (MQ=255) gcggcgGCTGTCTGCCAGTTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGT‑CATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAAt < 1:296838/146‑1 (MQ=255) ggcggcTTTCTCCCAGTTTTTTTCCCGCCGAAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGT‑CATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCt < 1:225570/149‑1 (MQ=255) ccggtttttttcCCGCCCCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGT‑CATGTTGTGTGGCAATCGCTGCTGGTAACAATGTAGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCt < 1:516877/143‑1 (MQ=255) ccAGCTTTTTTCCCGCCAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGT‑CATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAAcc < 2:896195/145‑1 (MQ=255) ttttttcCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGT‑CATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTg < 1:841908/148‑1 (MQ=255) | CGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCA‑TGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAGAAATAGCTTTAAGCCCGT‑CATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTG > NC_000913/357733‑358023 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |