Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,356,671	T→G	Y147S (TAC→TCC)	sapA ←	antimicrobial peptide transport ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,356,671	0	T	G	100.0%	215.7 / NA	56	Y147S (TAC→TCC)	sapA	antimicrobial peptide transport ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (29/27);  total (29/27)

ATATTCTGCCGACATGACCGAAGCATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGG  >  NC_000913/1356526‑1356817                                                                                                                                                  |                                                                                                                                                   atatTCTGCCGACATGACCGAAGCATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGGAgggg                                                                                                                                               >  1:556246/1‑151 (MQ=255)       tGCCGACATGACCGAAGCATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGGAGGGGAAGTtg                                                                                                                                         >  2:1006867/1‑151 (MQ=255)        gCCGACATGACCGAAGCATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGGAGGGGAAGTtgc                                                                                                                                        >  1:1103780/1‑151 (MQ=255)         ccGACATGACCGAAGCATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGGAGGGGAAGTtgtt                                                                                                                                       >  2:551521/1‑149 (MQ=255)          cGACATGACCGAAGCATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGGAGGGGAAGTtgctg                                                                                                                                      >  1:959647/1‑151 (MQ=255)             cATGACCGAAGCATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGGAGGGGAAGTTGCTGCCg                                                                                                                                   <  1:885601/151‑1 (MQ=255)             cATGACCGAAGCATAATGGGTTGCGAGGTGCCAC‑AAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGGAGGGGAAGTTGCTGCCGt                                                                                                                                  >  1:397383/1‑151 (MQ=255)               tGACCGAAGCATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGGAGGGGAAGTTGCTGCCGtt                                                                                                                                 >  2:503836/1‑151 (MQ=255)                gACCGAAGCATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGGAGGGGAAGTTGCTGCCGTTg                                                                                                                                <  1:175413/151‑1 (MQ=255)                  ccGAAGCATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGGAGGGGAAGTTGCTGCCGTTGAc                                                                                                                              <  1:1020680/151‑1 (MQ=255)                     aaGCATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGGAGGGGAAGTTGCTGCCGTTGACGtt                                                                                                                           >  1:1093914/1‑151 (MQ=255)                         aTAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGGAGGGGAAGTTGCTGCCGTTGACGTTATGc                                                                                                                       <  1:109618/151‑1 (MQ=255)                           aaTGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGGAGGGGAAGTTGCTGCCGTTGACGTTATGcc                                                                                                                      >  2:182892/1‑150 (MQ=255)                           aaTGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGGAGGGGAAGTTGCTGCCGTTGACGTTATGcc                                                                                                                      <  1:342394/150‑1 (MQ=255)                           aaTGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGGAGGGGAAGTTGCTGCCGTTGACGTTATGCCa                                                                                                                     <  2:524189/151‑1 (MQ=255)                            aTGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGGAGGGGAAGTTGCTGCCGTTGACGTTATGCCAc                                                                                                                    <  1:819605/151‑1 (MQ=255)                             tGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGGAGGGGAAGTTGCTGCCGTTGACGTTATGCCAc                                                                                                                    <  1:486523/150‑1 (MQ=255)                                gTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGGAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGgt                                                                                                                >  1:320948/1‑151 (MQ=255)                                  tGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGGAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGgtt                                                                                                               <  2:401472/150‑1 (MQ=255)                                               aaaaaaGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGGAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCaaaa                                                                                                  <  2:257299/150‑1 (MQ=255)                                                 aaaaGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGGAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAAtt                                                                                               >  2:15891/1‑151 (MQ=255)                                                 aaaaGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGGAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAAtt                                                                                               <  2:909540/151‑1 (MQ=255)                                                  aaaGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGGAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTc                                                                                              <  1:332630/151‑1 (MQ=255)                                                          aTCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGGAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGa                                                                                        >  1:157549/1‑149 (MQ=255)                                                            ccGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGGAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAgg                                                                                     >  1:453840/1‑150 (MQ=255)                                                              ggCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGGAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTaa                                                                                  <  1:641302/151‑1 (MQ=255)                                                               gCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGGAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTaaa                                                                                 <  2:840205/151‑1 (MQ=255)                                                                     cGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGGAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAAcaccac                                                                           >  2:1115936/1‑151 (MQ=255)                                                                        gTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGGAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACAtc                                                                        <  2:176906/151‑1 (MQ=255)                                                                                  aaCGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGGAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGcattc                                                              <  2:72816/151‑1 (MQ=255)                                                                                  aaCGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGGAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGcattc                                                              >  2:260336/1‑151 (MQ=255)                                                                                        aTGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGGAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATAGTCGGCATTCAtttt                                                         <  1:452613/150‑1 (MQ=255)                                                                                          gATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGGAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACg                                                      <  1:437467/151‑1 (MQ=255)                                                                                              aTCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGGAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTg                                                  <  2:777388/151‑1 (MQ=255)                                                                                                   gTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGGAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAg                                              >  1:132671/1‑150 (MQ=255)                                                                                                   gTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGGAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGt                                             <  1:1045031/151‑1 (MQ=255)                                                                                                   gTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGGAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGt                                             >  1:570017/1‑151 (MQ=255)                                                                                                     ttGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGGAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTa                                            >  2:1105882/1‑150 (MQ=255)                                                                                                       gCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGGAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAAc                                         <  2:479430/151‑1 (MQ=255)                                                                                                       gCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGGAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGCTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTaaa                                          >  2:1085704/1‑150 (MQ=255)                                                                                                           aCGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGGAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGt                                     >  1:450123/1‑151 (MQ=255)                                                                                                               ttttCACGTTATCGGCAAATTGCAGGCTGTCGAAGGAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGt                                 >  2:1092843/1‑151 (MQ=255)                                                                                                                  tCACGTTATCGGCAAATTGCAGGCTGTCGAAAGAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGttt                               <  1:673021/150‑1 (MQ=255)                                                                                                                   cACGTTATCGGCAAATTGCAGGCTGTCGAAGGAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGGGTAAACCAGTCGGtttt                              <  1:1092057/150‑1 (MQ=255)                                                                                                                   cACGTTATCGGCAAATTGCAGGCTGTCGAAGGAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGtttt                              <  2:818103/150‑1 (MQ=255)                                                                                                                   cACGTTATCGGCAAATTGCAGGCTGTCGAAGGAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGtttt                              <  1:219839/150‑1 (MQ=255)                                                                                                                      gTTATCGGCAAATTGCAGGCTGTCGAAGGAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTg                           >  1:159541/1‑150 (MQ=255)                                                                                                                      gTTATCGGCAAATTGCAGGCTGTCGAAGGAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTg                           >  1:378198/1‑150 (MQ=255)                                                                                                                       ttATCGGCAAATTGCAGGCTGTCGAAGGAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGa                          >  2:253352/1‑150 (MQ=255)                                                                                                                         aTCGGCAAATTGCAGGCTGTCGAAGGAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAAc                       <  1:1006989/151‑1 (MQ=255)                                                                                                                             gCAAATTGCAGGCTGTCGAAGGAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGa                    >  1:610654/1‑150 (MQ=255)                                                                                                                              cAAATTGCAGGCTGTCGAAGGAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGCAAACCAGTCGGTTTTTTGTAACGGAAc                  >  2:201304/1‑151 (MQ=255)                                                                                                                               aaaTTGCAGGCTGTCGAAGGAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAAc                  >  2:568992/1‑150 (MQ=255)                                                                                                                                 aTTGCAGGCTGTCGAAGGAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATc               >  1:531171/1‑151 (MQ=255)                                                                                                                                           gTCGAAGGAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAgg     <  1:591966/151‑1 (MQ=255)                                                                                                                                              gAAGGAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTgg  >  2:794805/1‑151 (MQ=255)                                                                                                                                                  |                                                                                                                                                   ATATTCTGCCGACATGACCGAAGCATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATCGGCAAATTGCAGGCTGTCGAAGTAGGGGAAGTTGCTGCCGTTGACGTTATGCCACGGGTTGTTGCGGTCAAAAATTCGCTGGAAGGTAAACACCACATCGTCGGCATTCATTTTACGAGTGGGAGTAAACCAGTCGGTTTTTTGAAACGGAACATCGCGACGCAGGTGG  >  NC_000913/1356526‑1356817

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑