Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,583,769	T→G	Q64P (CAA→CCA)	safA ←	two‑component system connector membrane protein, EvgSA to PhoQP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,583,769	0	T	G	100.0%	303.8 / NA	73	Q64P (CAA→CCA)	safA	two‑component system connector membrane protein, EvgSA to PhoQP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (39/34);  total (39/34)

TTATCTAAAATGTTAGCGTTGAAGGCATGATGAATAAATATAACAGAACAAACGAGCGACATTTTATCTCCTTAAAACAATAAAGTTTTTATCGATACTTTTTTAACATTTCATATTTATAATTTGCTGTTTGTTTTCAGCCTTGCAAACTATTGATAATGAAATGTGTAAGATCTCTTGCAATGCGACCCATTTCTGAAATACTCAGCTGATCAATTGCCGAAAAAATGGCTATCAACGTAAGTGTCAGTAATAAGACAACCACAATTGCAGACATGATTTCTTTAT  >  NC_000913/1583626‑1583913                                                                                                                                                |                                                                                                                                                 ttATCTAAAATGTTAGCGTTGAAGGCATGATGAATAAATATAACAGAACAAACGAGCGACATTTTATCTCCTTAAAACAATAAAGTTTTTATCGATACTTTTTTAACATTTCATATTTATAATTTGCTGTTTGTTTTCAGCCTGGCaaa                                                                                                                                             <  2:861045/149‑1 (MQ=255)  tATCTAAAATGTTAGCGTTGAAGGCATGATGAATAAATATAACAGAACAAACGAGCGACATTTTATCTCCTTAAAACAATAAAGTTTTTATCGATACTTTTTTAACATTTCATATTTATAATTTGCTGTTTGTTTTCAGCCTGGCAAACt                                                                                                                                           >  2:496877/1‑150 (MQ=255)  tATCTAAAATGTTAGCGTTGAAGGCATGATGAATAAATATAACAGAACAAACGAGCGACATTTTATCTCCTTAAAACAATAAAGTTTTTATCGATACTTTTTTAACATTTCATATTTATAATTTGCTGTTTGTTTTCAGCCTGGCAAACt                                                                                                                                           <  1:125148/150‑1 (MQ=255)   aTCTAAAATGTTAGCGTTGAAGGCATGATGAATAAATATAACAGAACAAACGAGCGACATTTTATCTCCTTAAAACAATAAAGTTTTTATCGATACTTTTTTAACATTTCATATTTATAATTTGCTGTTTGTTTTCAGCCTGGCAAACTa                                                                                                                                          <  2:391467/150‑1 (MQ=255)     cTAAAATGTTAGCGTTGAAGGCATGATGAATAAATATAACAGAACAAACGAGCGACATTTTATCTCCTTAAAACAATAAAGTTTTTATCGATACTTTTTTAACATTTCATATTTATAATTTGCTGTTTGTTTTCAGCCTGGCAAACTAtt                                                                                                                                        >  2:392644/1‑150 (MQ=255)               aGCGTTGAAGGCATGATGAATAAATATAACAGAACAAACGAGCGACATTTTATCTCCTTAAAACAATAAAGTTTTTATCGATACTTTTTTAACATTTCATATTTATAATTTGCTGTTTGTTTTCAGCCTGGCAAACTATTGATAATGaaa                                                                                                                              <  2:382636/150‑1 (MQ=255)                gCGTTGAAGGCATGATGAATAAATATAACAGAACAAACGAGCGACATTTTATCTCCTTAAAACAATAAAGTTTTTATCGATACTTTTTTAACATTTCATATTTATAATTTGCTGTTTGTTTTCAGCCTGGCAAACTATTGATAATGAAAtg                                                                                                                            >  2:468808/1‑151 (MQ=255)                    tGAAGGCATGATGAATAAATATAACAGAACAAACGAGCGACATTTTATCTCCTTAAAACAATAAAGTTTTTATCGATACTTTTTTAACATTTCATATTTATAATTTGCTGTTTGTTTTCAGCCTGGCAAACTATTGATAATGAAATgtgt                                                                                                                         >  2:637768/1‑150 (MQ=255)                    tGAAGGCATGATGAATAAATATAACAGAACAAACGAGCGACATTTTATCTCCTTAAAACAATAAAGTTTTTATCGATACTTTTTTAACATTTCATATTTATAATTTGCTGTTTGTTTTCAGCCTGGCAAACTATTGATAATGAAATgtgt                                                                                                                         >  2:752776/1‑150 (MQ=255)                     gAAGGCATGATGAGTAAATATAACAGAACAAACGAGCGACATTTTATCTCCTTAAAACAATAAAGTTTTTATCGATACTTTTTTAACATTTCATATTTATAATTTGCTGTTTGTTTTCAGCCTGGCAAACTATTGATAATGAAATGTGTa                                                                                                                        >  2:1005674/1‑150 (MQ=255)                         gCATGATGAATAAATATAACAGAACAAACGAGCGACATTTTATCTCCTTAAAACAATAAAGTTTTTATCGATACTTTTTTAACATTTCATATTTATAATTTGCTGTTTGTTTTCAGCCTGGCAAACTATTGATAATGAAATGTGTa                                                                                                                        >  1:214003/1‑146 (MQ=255)                         gCATGATGAATAAATATAACAGAACAAACGAGCGACATTTTATCTCCTTAAAACAATAAAGTTTTTATCGATACTTTTTTAACATTTCATATTTATAATTTGCTGTTTGTTTTCAGCCTGGCAAACTATTGATAATGAAATGTGTAAGAtc                                                                                                                   <  2:984426/151‑1 (MQ=255)                          cATGATGAATAAATATAACAGAACAAACGAGCGACATTTTATCTCCTTAAAACAATAAAGTTTTTATCGATACTTTTTTAACATTTCATATTTATAATTTGCTGTTTGTTTTCAGCCTGGCAAACTATTGATAATGAAATGTGTAAGAtct                                                                                                                  <  1:985365/151‑1 (MQ=255)                            tgatgaATAAATATAACAGAACAAACGAGCGACATTTTATCTCCTTAAAACAATAAAGTTTTTATCGATACTTTTTTAACATTTCATATTTATAATTTGCTGTTTGTTTTCAGCCTGGCAAACTATTGATAATGAAATGTGTAAGATctct                                                                                                                >  2:844369/1‑151 (MQ=255)                              atgaATAAATATAACAGAACAAACGAGCGACATTTTATCTCCTTAAAACAATAAAGTTTTTATCGATACTTTTTTAACATTTCATATTTATAATTTGCTGTTTGTTTTCAGCCTGGCAAACTATTGATAATGAAATGTGTAAGATCTCTTg                                                                                                              >  1:66695/1‑151 (MQ=255)                                gaATAAATATAACAGAACAAACGAGCGACATTTTATCTCCTTAAAACAATAAAGTTTTTATCGATACTTTTTTAACATTTCATATTTATAATTTGCTGTTTGTTTTCAGCCTGGCAAACTATTGATAATGAAATGTGTAAGATCTCTTGc                                                                                                             >  1:884912/1‑150 (MQ=255)                                gaATAAATATAACAGAACAAACGAGCGACATTTTATCTCCTTAAAACAATAAAGTTTTTATCGATACTTTTTTAACATTTCATATTTATAATTTGCTGTTTGTTTTCAGCCTGGCAAACTATTGATAATGAAATGTGTAAGATCTCTTGc                                                                                                             >  1:650782/1‑150 (MQ=255)                                  ataaataTAACAGAACAAACGAGCGACATTTTATCTCCTTAAAACAATAAAGTTTTTATCGATACTTTTTTAACATTTCATATTTATAATTTGCTGTTTGTTTTCAGCCTGGCAAACTATTGATAATGAAATGTGTAAGATCTCTTGCAAt                                                                                                          >  1:862758/1‑151 (MQ=255)                                  ataaataTAACAGAACAAACGAGCGACATTTTATCTCCTTAAAACAATAAAGTTTTTATCGATACTTTTTTAACATTTCATATTTATAATTTGCTGTTTGTTTTCAGCCTGGCAAACTATTGATAATGAAATGTGTAAGATCTCTTGCAAt                                                                                                          <  1:1111354/151‑1 (MQ=255)                                    aaataTAACAGAACAAACGAGCGACATTTTATCTCCTTAAAACAATAAAGTTTTTATCGATACTTTTTTAACATTTCATATTTATAATTTGCTGTTTGTTTTCAGCCTGGCAAACTATTGATAATGAAATGTGTAAGATCTCTTGCAATg                                                                                                         <  1:449251/150‑1 (MQ=255)                                    aaataTAACAGAACAAACGAGCGACATTTTATCTCCTTAAAACAATAAAGTTTTTATCGATACTTTTTTAACATTTCATATTTATAATTTGCTGTTTGTTTTCAGCCTGGCAAACTATTGATAATGAAATGTGTAAGATCTCTTGCAATGc                                                                                                        >  1:386198/1‑151 (MQ=255)                                    aaataTAACAGAACAAACGAGCGACATTTTATCTCCTTAAAACAATAAAGTTTTTATCGATACTTTTTTAACATTTCATATTTATAATTTGCTGTTTGTTTTCAGCCTGGCAAACTATTGATAATGAAATGTGTAAGATCTCTTGCAATGc                                                                                                        <  2:945300/151‑1 (MQ=255)                                    aaataTAACAGAACAAACGAGCGACATTTTATCTCCTTAAAACAATAAAGTTTTTATCGATACTTTTTTAACATTTCATATTTATAATTTGCTGTTTGTTTTCAGCCTGGCAAACTATTGATAATGAAATGTGTAAGATCTCTTGCAATGc                                                                                                        <  1:662654/151‑1 (MQ=255)                                        ataACAGAACAAACGAGCGACATTTTATCTCCTTAAAACAATAAAGTTTTTATCGATACTTTTTTAACATTTCATATTTATAATTTGCTGTTTGTTTTCAGCCTGGCAAACTATTGATAATGAAATGTGTAAGATCTCTTGCAATGCGAcc                                                                                                    <  2:100842/151‑1 (MQ=255)                                             aGAACAAACGAGCGACATTTTATCTCCTTAAAACAATAAAGTTTTTATCGATACTTTTTTAACATTTCATATTTATAATTTGCTGTTTGTTTTCAGCCTGGCAAACTATTGATAATGAAATGTGTAAGATCTCTTGCAATGCGACCCAttt                                                                                               <  1:172369/151‑1 (MQ=255)                                                  aaaCGAGCGACATTTTATCTCCTTAAAACAATAAAGTTTTTATCGATACTTTTTTAACATTTCATATTTATAATTTGCTGTTTGTTTTCAGCCTGGCAAACTATTGATAATGAAATGTGTAAGATCTCTTGCAATGCGACCCATTTCTGa                                                                                           >  2:1021874/1‑150 (MQ=255)                                                   aaCGAGCGACATTTTATCTCCTTAAAACAATAAAGTTTTTATCGATACTTTTTTAACATTTCATATTTATAATTTGCTGTTTGTTTTCAGCCTGGCAAACTATTGATAATGAAATGTGTAAGATCTCTTGCAATGCGACCCATTTCTGaa                                                                                          >  2:453825/1‑150 (MQ=255)                                                         cGACATTTTATCTCCTTAAAACAATAAAGTTTTTATCGATACTTTTTTAACATTTCATATTTATAATTTGCTGTTTGTTTTCAGCCTGGCAAACTATTGATAATGAAATGTGTAAGATCTCTTGCAATGCGACCCATTTCTGAAATACTCa                                                                                   <  1:439672/151‑1 (MQ=255)                                                           aCATTTTATCTCCTTAAAACAATAAAGTTTTTATCGATACTTTTTTAACATTTCATATTTATAATTTGCTGTTTGTTTTCAGCCTGGCAAACTATTGATAATGAAATGTGTAAGATCTCTTGCAATGCGACCCATTTCTGAAATACTCAGc                                                                                 >  1:275790/1‑151 (MQ=255)                                                                 tATCTCCTTAAAACAATAAAGTTTTTATCGATACTTTTTTAACATTTCATATTTATAATTTGCTGTTTGTTTTCAGCCTGGCAAACTATTGATAATGAAATGTGTAAGATCTCTTGCAATGCGACCCATTTCTGAAATACTCAGCTGATc                                                                            <  1:1119744/150‑1 (MQ=255)                                                                 tATCTCCTTAAAACAATAAAGTTTTTATCGATACTTTTTTAACATTTCATATTTATAATTTGCTGTTTGTTTTCAGCCTGGCAAACTATTGATAATGAAATGTGTAAGATCTCTTGCAATGCGACCCATTTCTGAAATACTCAGCTGATc                                                                            >  1:813034/1‑150 (MQ=255)                                                                  aTCTCCTTAAAACAATAAAGTTTTTATCGATACTTTTTTAACATTTCATATTTATAATTTGCTGTTTGTTTTCAGCCTGGCAAACTATTGATAATGAAATGTGTAAGATCTCTTGCAATGCGACCCATTTCTGAAATACTCAGCTGATCa                                                                           >  1:1120315/1‑150 (MQ=255)                                                                  aTCTCCTTAAAACAATAAAGTTTTTATCGATACTTTTTTAACATTTCATATTTATAATTTGCTGTTTGTTTTCAGCCTGGCAAACTATTGATAATGAAATGTGTAAGATCTCTTGCAATGCGACCCATTTCTGAAATACTCAGCTGATCa                                                                           <  1:597595/150‑1 (MQ=255)                                                                  aTCTCCTTAAAACAATAAAGTTTTTATCGATACTTTTTTAACATTTCATATTTATAATTTGCTGTTTGTTTTCAGCCTGGCAAACTATTGATAATGAAATGTGTAAGATCTCTTGCAATGCGACCCATTTCTGAAATACTCAGCTGATCa                                                                           >  1:253572/1‑150 (MQ=255)                                                                      ccTTAAAACAATAAAGTTTTTATCGATACTTTTTTAACATTTCATATTTATAATTTGCTGTTTGTTTTCAGCCTGGCAAACTATTGATAATGAAATGTGTAAGATCTCTTGCAATGCGACCCATTTCTGAAATACTCAGCTGATCAAtt                                                                        <  2:301955/149‑1 (MQ=255)                                                                        ttAAAACAATAAAGTTTTTATCGATACTTTTTTAACATTTCATATTTATAATTTGCTGTTTGTTTTCAGCCTGGCAAACTATTGATAATGAAATGTGTAAGATCTCTTGCAATGCGACCCATTTCTGAAATACTCAGCTGATCAATTGCCg                                                                    >  1:967576/1‑151 (MQ=255)                                                                         tAAAACAATAAAGTTTTTATCGATACTTTTTTAACATTTCATATTTATAATTTGCTGTTTGTTTTCAGCCTGGCAAACTATTGATAATGAAATGTGTAAGATCTCTTGCAATGCGACCCATTTCTGAAATACTCAGCTGATCAATTGCCg                                                                    >  1:479428/1‑150 (MQ=255)                                                                             aCAATAAAGTTTTTATCGATACTTTTTTAACATTTCATATTTATAATTTGCTGTTTGTTTTCAGCCTGGCAAACTATTGATAATGAAATGTGTAAGATCTCTTGCAATGCGACCCATTTCTGAAATACTCAGCTGATCAATTGCCGaaaaa                                                               <  1:62350/151‑1 (MQ=255)                                                                             aCAATAAAGTTTTTATCGATACTTTTTTAACATTTCATATTTATAATTTGCTGTTTGTTTTCAGCCTGGCAAACTATTGATAATGAAATGTGTAAGATCTCTTGCAATGCGACCCATTTCTGAAATACTCAGCTGATCAATTGCCGaaaaa                                                               <  2:293196/151‑1 (MQ=255)                                                                               aaTAAAGTTTTTATCGATACTTTTTTAACATTTCATATTTATAATTTGCTGTTTGTTTTCAGCCTGGCAAACTATTGATAATGAAATGTGTAAGATCTCTTGCAATGCGACCCATTTCTGAAATACTCAGCTGATCAATTGCCGAAAAAAt                                                             <  2:461818/151‑1 (MQ=255)                                                                                 tAAAGTTTTTATCGATACTTTTTTAACATTTCATATTTATAATTTGCTGTTTGTTTTCAGCCTGGCAGACTATTGATAATGAAATGTGTAAGATCTCTTGCAATGCGACCCATTTCTGAAATACTCAGCTGATCAATTGCCGAAAAAATgg                                                           >  2:294187/1‑151 (MQ=255)                                                                                 tAAAGTTTTTATCGATACTTTTTTAACATTTCATATTTATAATTTGCTGTTTGTTTTCAGCCTGGCAAACTATTGATAATGAAATGTGTAAGATCTCTTGCAATGCGACCCATTTCTGAAATACTCAGCTGATCAATTGCCGAAAAAATgg                                                           >  1:6029/1‑151 (MQ=255)                                                                                  aaaGTTTTTATCGATACTTTTTTAACATTTCATATTTATAATTTGCTGTTTGTTTTCAGCCTGGCAAACTATTGATAATGAAATGTGTAAGATCTCTTGCAATGCGACCCATTTCTGAAATACTCAGCTGATCAATTGCCGAAAAAATGGc                                                          <  1:674597/151‑1 (MQ=255)                                                                                  aaaGTTTTTATCGATACTTTTTTAACATTTCATATTTATAATTTGCTGTTTGTTTTCAGCCTGGCAAACTATTGATAATGAAATGTGTAAGATCTCTTGCAATGCGACCCATTTCTGAAATACTCAGCTGATCAATTGCCGAAAAAATGGc                                                          <  2:190450/151‑1 (MQ=255)                                                                                       ttttATCGATACTTTTTTAACATTTCATATTTATAATTTGCTGTTTGTTTTCAGCCTGGCAAACTATTGATAATGAAATGTGTAAGATCTCTTGCAATGCGACCCATTTCTGAAATACTCAGCTGATCAATTGCCGAAAAAATGGCTATc                                                      <  1:684297/150‑1 (MQ=255)                                                                                       ttttATCGATACTTTTTTAACATTTCATATTTATAATTTGCTGTTTGTTTTCAGCCTGGCAAACTATTGATAATGAAATGTGTAAGATCTCTTGCAATGCGACCCATTTCTGAAATACTCAGCTGATCAATTGCCGAAAAAATGGCTATc                                                      >  1:452587/1‑150 (MQ=255)                                                                                        tttATCGATACTTTTTTAACATTTCATATTTATAATTTGCTGTTTGTTTTCAGCCTGGCAAACTATTGATAATGAAATGTGTAAGATCTCTTGCAATGCGACCCATTTCTGAAATACTCAGCTGATCAATTGCCGAAAAAATGGCTATCa                                                     <  2:268425/150‑1 (MQ=255)                                                                                             cGATACTTTTTTAACATTTCATATTTATAATTTGCTGTTTGTTTTCAGCCTGGCAAACTATTGATAATGAAATGTGTAAGATCTCTTGCAATGCGACCCATTTCTGAAATACTCAGCTGATCAATTGCCGAAAAAATGGCTATCAACGTa                                                <  1:963862/150‑1 (MQ=255)                                                                                             cGATACTTTTTTAACATTTCATATTTATAATTTGCTGTTTGTTTTCAGCCTGGCAAACTATTGATAATGAAATGTGTAAGATCTCTTGCAATGCGACCCATTTCTGAAATACTCAGCTGATCAATTGCCGAAAAAATGGCTATCAACGTa                                                >  2:196932/1‑150 (MQ=255)                                                                                               aTACTTTTTTAACATTTCATATTTATAATTTGCTGTTTGTTTTCAGCCTGGCAAACTATTGATAATGAAATGTGTAAGATCTCTTGCAATGCGACCCATTTCTGAAATACTCAGCTGATCAATTGCCGAAAAAATGGCTATCAACGTAAg                                              >  1:1045777/1‑150 (MQ=255)                                                                                                 aCTTTTTTAACATTTCATATTTATAATTTGCTGTTTGTTTTCAGCCTGGCAAACTATTGATAATGAAATGTGTAAGATCTCTTGCAATGCGACCCATTTCTGAAATACTCAGCTGATCAATTGCCGAAAAAATGGCTATCAACGTAAgtgt                                           <  2:795503/151‑1 (MQ=255)                                                                                                 aCTTTTTTAACATTTCATATTTATAATTTGCTGTTTGTTTTCAGCCTGGCAAACTATTGATAATGAAATGTGTAAGATCTCTTGCAATGCGACCCATTTCTGAAATACTCAGCTGATCAATTGCCGAAAAAATGGCTATCAACGTAAgtgt                                           >  1:977921/1‑151 (MQ=255)                                                                                                  cTTTTTTAACATTTCATATTTATAATTTGCTGTTTGTTTTCAGCCTGGCAAACTATTGATAATGAAATGTGTAAGATCTCTTGCAATGCGACCCATTTCTGAAATACTCAGCTGATCAATTGCCGAAAAAATGGCTATCAACGTAAGTGTc                                          >  2:48348/1‑151 (MQ=255)                                                                                                    tttttAACATTTCATATTTATAATTTGCTGTTTGTTTTCAGCCTGGCAAACTATTGATAATGAAATGTGTAAGATCTCTTGCAATGCGACCCATTTCTGAAATACTCAGCTGATCAATTGCCGAAAAAATGGCTATCAACGTAAGTGTCa                                         <  2:305745/150‑1 (MQ=255)                                                                                                     ttttAACATTTCATATTTATAATTTGCTGTTTGTTTTCAGCCTGGCAAACTATTGATAATGAAATGTGTAAGATCTCTTGCAATGCGACCCATTTCTGAAATACTCAGCTGATCAATTGCCGAAAAAATGGCTATCAACGTAAGTGTCAg                                        <  1:720227/150‑1 (MQ=255)                                                                                                          aCATTTCATATTTATAATTTGCTGTTTGTTTTCAGCCTGGCAAACTATTGATAATGAAATGTGTAAGATCTCTTGCAATGCGACCCATTTCTGAAATACTCAGCTGATCAATTGCCGAAAAAATGGCTATCAACGTAAGTGTCAGtaata                                   >  1:920989/1‑150 (MQ=255)                                                                                                           cATTTCATATTTATAATTTGCTGTTTGTTTTCAGCCTGGCAAACTATTGATAATGAAATGTGTAAGATCTCTTGCAATGCGACCCATTTCTGAAATACTCAGCTGATCAATTGCCGAAAAAATGGCTATCAACGTAAGTGTCAGtaata                                   >  2:1000977/1‑149 (MQ=255)                                                                                                            aTTTCATATTTATAATTTGCTGTTTGTTTTCAGCCTGGCAAACTATTGATAATGAAATGTGTAAGATCTCTTGCAATGCGACCCATTTCTGAAATACTCAGCTGATCAATTGCCGAAAAAATGGCTATCAACGTAAGTGTCAGtaata                                   >  1:755078/1‑148 (MQ=255)                                                                                                               tCATATTTATAATTTGCTGTTTGTTTTCAGCCTGGCAAACTATTGATAATGAAATGTGTAAGATCTCTTGCAATGCGACCCATTTCTGAAATACTCAGCTGATCAATTGCCGAAAAAATGGCTATCAACGTAAGTGTCAGTAATAAGACa                              <  1:372586/150‑1 (MQ=255)                                                                                                                cATATTTATAATTTGCTGTTTGTTTTCAGCCTGGCAAACTATTGATAATGAAATGTGTAAGATCTCTTGCAATGCGACCCATTTCTGAAATACTCAGCTGATCAATTGCCGAAAAAATGGCTATCAACGTAAGTGTCAGTAATAAGACAAc                            <  2:980714/151‑1 (MQ=255)                                                                                                                  tatTTATAATTTGCTGTTTGTTTTCAGCCTGGCAAACTATTGATAATGAAATGTGTAAGATCTCTTGCAATGCGACCCATTTCTGAAATACTCAGCTGATCAATTGCCGAAAAAATGGCTATCAACGTAAGTGTCAGTAATAAGACAAcc                           >  1:831623/1‑150 (MQ=255)                                                                                                                    tttATAATTTGCTGTTTGTTTTCAGCCTGGCAAACTATTGATAATGAAATGTGTAAGATCTCTTGCAATGCGACCCATTTCTGAAATACTCAGCTGATCAATTGCCGAAAAAATGGCTATCAACGTAAGTGTCAGTAATAAGACAACcac                         >  1:109467/1‑150 (MQ=255)                                                                                                                     ttATAATTTGCTGTTTGTTTTCAGCCTGGCAAACTATTGATAATGAAATGTGTAAGATCTCTTGCAATGCGACCCATTTCTGAAATACTCAGCTGATCAATTGCCGAAAAAATGGCTATCAACGTAAGTGTCAGTAATAAGACAACcaca                        <  2:1036733/150‑1 (MQ=255)                                                                                                                           tttGCTGTTTGTTTTCAGCCTGGCAAACTATTGATAATGAAATGTGTAAGATCTCTTGCAATGCGACCCATTTCTGAAATACTCAGCTGATCAATTGCCGAAAAAATGGCTATCAATGTAAGTGTCAGTAATAAGACAACCACAAt                      >  2:315912/1‑146 (MQ=255)                                                                                                                               cTGTTTGTTTTCAGCCTGGCAAACTATTGATAATGAAATGTGTAAGATCTCTTGCAATGCGACCCATTTCTGAAATACTCAGCTGATCAATTGCCGAAAAAATGGCTATCAACGTAAGTGTCAGTAATAAGACAACCACAATTGCAGACAt             >  2:189449/1‑151 (MQ=255)                                                                                                                                tgtttgttTTCAGCCTGGCAAACTATTGATAATGAAATGTGTAAGATCTCTTGCAATGCGACCCATTTCTGAAATACTCAGCTGATCAATTGCCGAAAAAATGGCTATCAACGTAAGTGTCAGTAATAAGACAACCACAATTGCAGACATg            >  2:332600/1‑151 (MQ=255)                                                                                                                                 gtttgtttTCAGCCTGGCAAACTATTGATAATGAAATGTGTAAGATCTCTTGCAATGCGACCCATTTCTGAAATACTCAGCTGATCAATTGCCGAAAAAATGGCTATCAACGTAAGTGTCAGTAATAAGACAACCACAATTGCAGACATGa           <  1:291109/151‑1 (MQ=255)                                                                                                                                   ttgtttTCAGCCTGGCAAACTATTGATAATGAAATGTGTAAGATCTCTTGCAATGCGACCCATTTCTGAAATACTCAGCTGATCAATTGCCGAAAAAATGGCTATCAACGTAAGTGTCAGTAATAAGACAACCACAATTGCAGACATGAt          <  1:752882/150‑1 (MQ=255)                                                                                                                                    tgtttTCAGCCTGGCAAACTATTGATAATGAAATGTGTAAGATCTCTTGCAATGCGACCCATTTCTGAAATACTCAGCTGATCAATTGCCGAAAAAATGGCTATCAACGTAAGTGTCAGTAATAAGACAACCACAATTGCAGACATGAtt         <  2:292021/150‑1 (MQ=255)                                                                                                                                     gtttTCAGCCTGGCAAACTATTGATAATGAAATGTGTAAGATCTCTTGCAATGCGACCCATTTCTGAAATACTCAGCTGATCAATTGCCGAAAAAATGGCTATCAACGTAAGTGTCAGTAATAAGACAACCACAATTGCAGACATGAttt        <  2:66689/150‑1 (MQ=255)                                                                                                                                      ttttCAGCCTGGCAAACTATTGATAATGAAATGTGTAAGATCTCTTGCAATGCGACCCATTTCTGAAATACTCAGCTGATCAATTGCCGAAAAAATGGCTATCAACGTAAGTGTCAGTAATAAGACAACCACAATTGCAGACATGATTTCt      >  2:253616/1‑151 (MQ=255)                                                                                                                                           aGCCTGGCAAACTATTGATAATGAAATGTGTAAGATCTCTTGCAATGCGACCCATTTCTGAAATACTCAGCTGATCAATTGCCGAAAAAATGGCTATCAACGTAAGTGTCAGTAATAAGACAACCACAATTGCAGACATGATTTCTTtat  >  2:908844/1‑150 (MQ=255)                                                                                                                                           aGCCTGGCAAACTATTGATAATGAAATGTGTAAGATCTCTTGCAATGCGACCCATTTCTGAAATACTCAGCTGATCAATTGCCGAAAAAATGGCTATCAACGTAAGTGTCAGTAATAAGACAACCACAATTGCAGACATGATTTCTTtat  >  1:1064774/1‑150 (MQ=255)                                                                                                                                                |                                                                                                                                                 TTATCTAAAATGTTAGCGTTGAAGGCATGATGAATAAATATAACAGAACAAACGAGCGACATTTTATCTCCTTAAAACAATAAAGTTTTTATCGATACTTTTTTAACATTTCATATTTATAATTTGCTGTTTGTTTTCAGCCTTGCAAACTATTGATAATGAAATGTGTAAGATCTCTTGCAATGCGACCCATTTCTGAAATACTCAGCTGATCAATTGCCGAAAAAATGGCTATCAACGTAAGTGTCAGTAATAAGACAACCACAATTGCAGACATGATTTCTTTAT  >  NC_000913/1583626‑1583913

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑