Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,756,083	T→C	L129P (CTG→CCG)	pykF →	pyruvate kinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,756,083	0	T	C	100.0%	238.8 / NA	62	L129P (CTG→CCG)	pykF	pyruvate kinase I
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (38/24);  total (38/24)

TCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGA  >  NC_000913/1755947‑1756227                                                                                                                                         |                                                                                                                                                 tctcTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCCGATCGGTATGGa                                                                                                                                      >  2:960510/1‑149 (MQ=255)   tctGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCCGATCGGTATGGAAGtt                                                                                                                                  >  2:79443/1‑151 (MQ=255)      gAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCCGATCGGTATGGAAGTTAc                                                                                                                                <  2:341254/150‑1 (MQ=255)        aaGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCCGATCGGTATGGAAGTTACCGc                                                                                                                             >  1:368837/1‑151 (MQ=255)          gCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCCGATCGGTATGGAAGTTACCgtc                                                                                                                            >  2:222753/1‑148 (MQ=255)          gCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCCGATCGGTATGGAAGTTACCGcc                                                                                                                            >  1:737208/1‑150 (MQ=255)             ggTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCCGATCGGTATGGAAGTTACCGCCATTg                                                                                                                        >  1:637791/1‑151 (MQ=255)             ggTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCCGATCGGTATGGAAGTTACCGCCATTg                                                                                                                        <  1:563112/151‑1 (MQ=255)                cAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCCGATCGGTATGGAAGTTACCGCCATTGa                                                                                                                       >  2:808003/1‑149 (MQ=255)                aaGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCCGATCGGTATGGAAGTTACCGCCATTGa                                                                                                                       >  2:500098/2‑149 (MQ=255)                 aGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCCGATCGGTATGGAAGTTACCGCCATTGAAgg                                                                                                                    >  2:1026288/1‑151 (MQ=255)                    ccTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCCGATCGGTATGGAAGTTACCGCCATTGAAGGTaa                                                                                                                 <  2:958084/151‑1 (MQ=255)                    ccTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCCGATCGGTATGGAAGTTACCGCCATTGAAGGTa                                                                                                                  >  1:727301/1‑150 (MQ=255)                       ttACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCCGATCGGTATGGAAGTTACCGCCATTGAAGGTAACa                                                                                                               <  1:711820/150‑1 (MQ=255)                        tACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCCGATCGGTATGGAAGTTACCGCCATTGAAGGTAACaa                                                                                                              >  1:213054/1‑150 (MQ=255)                         aCTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCCGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAg                                                                                                            <  2:651342/151‑1 (MQ=255)                         aCTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCCGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAg                                                                                                            <  1:151105/151‑1 (MQ=255)                             tCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCCGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTAt                                                                                                        >  2:612434/1‑151 (MQ=255)                                 cacTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCCGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGt                                                                                                    >  2:296675/1‑151 (MQ=255)                                   cTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCCGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTa                                                                                                   >  1:617615/1‑150 (MQ=255)                                    tGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCCGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTaa                                                                                                  >  2:722199/1‑150 (MQ=255)                                     gATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCCGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTaa                                                                                                  <  1:712370/149‑1 (MQ=255)                                     gATAAATCTGTTATCGGCAACAGCGAAATGGATGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCCGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTa                                                                                                   >  2:904660/1‑148 (MQ=255)                                        aaaTCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCCGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGc                                                                                             >  1:885963/1‑151 (MQ=255)                                        aaaTCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCCGAACGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTg                                                                                              >  2:334713/1‑150 (MQ=255)                                          aTCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCCGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTg                                                                                           >  1:725337/1‑151 (MQ=255)                                          aTCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCCGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTg                                                                                           >  2:255755/1‑151 (MQ=255)                                           tCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGCTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCCGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTg                                                                                           >  1:669217/1‑150 (MQ=255)                                                 aTCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCCGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACg                                                                                    <  2:178127/151‑1 (MQ=255)                                                 aTCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCCGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACg                                                                                    >  1:242465/1‑151 (MQ=255)                                                 aTCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCCGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACg                                                                                    <  2:507791/151‑1 (MQ=255)                                                    ggCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCCGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTg                                                                                 <  1:908879/151‑1 (MQ=255)                                                      cAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCCGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGAc                                                                               <  1:753779/151‑1 (MQ=255)                                                             gAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCCGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCg                                                                        <  1:1092502/151‑1 (MQ=255)                                                               aaTGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCCGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGa                                                                       >  2:461398/1‑150 (MQ=255)                                                                aTGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCCGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGaaa                                                                     <  1:1104776/151‑1 (MQ=255)                                                                  ggTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCCGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAAc                                                                   >  2:677645/1‑151 (MQ=255)                                                                     tGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCCGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACaa                                                                 >  2:571226/1‑150 (MQ=255)                                                                        ggTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCCGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGgt                                                             >  2:702359/1‑151 (MQ=255)                                                                          tAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCCGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGgtg                                                            >  2:453165/1‑150 (MQ=255)                                                                           aaCGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCCGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGgtgt                                                           <  1:208903/150‑1 (MQ=255)                                                                                  gAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCCGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGCGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAAcctgc                                                   >  1:146566/1‑151 (MQ=255)                                                                                     ggTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCCGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAAcctgcctg                                                <  2:922018/151‑1 (MQ=255)                                                                                              actGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCCGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTcc                                        >  1:939972/1‑150 (MQ=255)                                                                                              actGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCCGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTc                                         <  1:471966/149‑1 (MQ=255)                                                                                                 gACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCCGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTg                                    <  1:791917/151‑1 (MQ=255)                                                                                                      gtctgtTGGCAACACCGTACTGGTTGACGATGGTCCGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGctct                                >  2:1095714/1‑150 (MQ=255)                                                                                                      gtctgtTGGCAACACCGTACTGGTTGACGATGGTCCGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTg                               >  2:362923/1‑151 (MQ=255)                                                                                                        catttGCCAACACCGTACTGGTTGACGATGGTCCGCTCGGTATGGAATTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGATCCTGCCAGGCGTTTCCAATGCTCTg                               <  2:460000/146‑1 (MQ=255)                                                                                                         tgtTGGCAACACCGTACTGGTTGACGATGGTCCGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGcc                             >  1:356476/1‑150 (MQ=255)                                                                                                         tgtTGGCAACACCGTACTGGTTGACGATGGTCCGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGcc                             >  2:809469/1‑150 (MQ=255)                                                                                                         tgtTGGCAACACCGTACTGGTTGACGATGGTCCGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGcc                             >  1:123146/1‑150 (MQ=255)                                                                                                          gtTGGCAACACCGTACTGGTTGACGATGGTCCGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCa                            <  1:331101/150‑1 (MQ=255)                                                                                                          gtTGGCAACACCGTACTGGTTGACGATGGTCCGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCa                            <  2:846905/150‑1 (MQ=255)                                                                                                                aaCACCGTACTGGTTGACGATGGTCCGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTgg                     >  1:570645/1‑151 (MQ=255)                                                                                                                aaCACCGTACTGGTTGACGATGGTCCGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTgg                     <  1:249077/151‑1 (MQ=255)                                                                                                                   acCGTACTGGTTGACGATGGTCCGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTg                  <  2:1041979/151‑1 (MQ=255)                                                                                                                          tGGTTGACGATGGTCCGATCGGTATGGAAGTTACCGCCATTGACGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTGCCATTGCTCTGCCAGCACTGGCTGaaa               >  2:260415/1‑147 (MQ=255)                                                                                                                           ggTTGACGATGGTCCGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGAc          >  2:123140/1‑151 (MQ=255)                                                                                                                                  gATGGTCCGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAgg   <  1:647485/151‑1 (MQ=255)                                                                                                                                  gATGGTCCGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAgg   >  1:558452/1‑151 (MQ=255)                                                                                                                                   aTGGTCCGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGa  <  1:864515/151‑1 (MQ=255)                                                                                                                                         |                                                                                                                                                 TCTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGA  >  NC_000913/1755947‑1756227

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑