Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	100,657	T→G	I338M (ATT→ATG)	murG →	N‑acetylglucosaminyl transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	100,657	0	T	G	100.0%	263.1 / NA	65	I338M (ATT→ATG)	murG	N‑acetylglucosaminyl transferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (26/39);  total (26/39)

ACTGGAATGCGCTACCGCTGGAAAAAGCGGGCGCAGCCAAAATTATCGAGCAGCCACAGCTTAGCGTGGATGCTGTCGCCAACACCCTGGCCGGGTGGTCGCGAGAAACCTTATTAACCATGGCAGAACGCGCCCGCGCTGCATCCATTCCGGATGCCACCGAGCGAGTGGCAAATGAAGTGAGCCGGGTTGCCCGGGCGTAATTGTAGCGATGCCTTTTGCATCGTATGAATTTAAGAAGTTAATGGCGTAAAGAATGAATACACAACAATTGGCAAAACTGCGTTCCATC  >  NC_000913/100509‑100800                                                                                                                                                     |                                                                                                                                                aCTGGAATGCGCTACCGCTGGAAAAAGCGGGCGCAGCCAAAATTATCGAGCAGCCACAGCTTAGCGTGGATGCTGTCGCCAACACCCTGGCCGGGTGGTCGCGAGAAACCTTATTAACCATGGCAGAACGCGCCCGCGCTGCATCCATGcc                                                                                                                                               <  2:829285/151‑1 (MQ=255)    ggAATGCGCTACCGCTGGAAAAAGCGGGCGCAGCCAAAATTATCGAGCAGCCACAGCTTAGCGTGGATGCTGTCGCCAACACCCTGGCCGGGTGGTCGCGAGAAACCTTATTAACCATGGCAGAACGCGCCCGCGCTGCATCCATGCCg                                                                                                                                              <  1:105879/149‑1 (MQ=255)         gcgcTACCGCTGGAAAAAGCGGGCGCAGCCAAAATTATCGAGCAGCCACAGCTTAGCGTGGATGCTGTCGCCAACACCCTGGCCGGGTGGTCGCGAGAAACCTTATTAACCATGGCAGAACGCGCCCGCGCTGCATCCATGCCGGATGCCa                                                                                                                                       <  1:201660/151‑1 (MQ=255)           gcTACCGCTGGAAAAAGCGGGCGCAGCCAAAATTATCGAGCAGCCACAGCTTAGCGTGGATGCTGTCGCCAACACCCTGGCCGGGTGGTCGCGAGAAACCTTATTAACCATGGCAGAACGCGCCCGCGCTGCATCCATGCCGGATGCCAcc                                                                                                                                     >  2:746739/1‑151 (MQ=255)              aCCGCTGGAAAAAGCGGGCGCAGCCAAAATTATCGAGCAGCCACAGCTTAGCGTGGATGCTGTCGCCAACACCCTGGCCGGGTGGTCGCGAGAAACCTTATTAACCATGGCAGAACGCGCCCGCGCTGCATCCATGCCGGATGCCACcg                                                                                                                                    >  2:13593/1‑149 (MQ=255)                  cTGGAAAAAGCGGGCGCAGCCAAAATTATCGAGCAGCCACAGCTTAGCGTGGATGCTGTCGCCAACACCCTGGCCGGGTGGTCGCGAGAAACCTTATTAACCATGGCAGAACGCGCCCGCGCTGCATCCATGCCGGATGCCACcgagcg                                                                                                                                >  2:993520/1‑149 (MQ=255)                       aaaaGCGGGCGCAGCCAAAATTATCGAGCAGCCACAGCTTAGCGTGGATGCTGTCGCCAACACCCTGGCCGGGTGGTCGCGAGAAACCTTATTAACCATGGCAGAACGCGCCCGCGCTGCATCCATGCCGGATGCCACCGAGCGAGTGGCa                                                                                                                         <  2:148775/151‑1 (MQ=255)                         aaGCGGGCGCAGCCAAAATTATCGAGCAGCCACAGCTTAGCGTGGATGCTGTCGCCAACACCCTGGCCGGGTGGTCGCGAGAAACCTTATTAACCATGGCAGAACGCGCCCGCGCTGCATCCATGCCGGATGCCACCGAGCGAGTGGCaa                                                                                                                        >  1:237033/1‑150 (MQ=255)                             gggCGCAGCCAAAATTATCGAGCAGCCACAGCTTAGCGTGGATGCTGTCGCCAACACCCTGGCCGGGTGGTCGCGAGAAACCTTATTAACCATGGCAGAACGCGCCCGCGCTGCATCCATGCCGGATGCCACCGAGCGAGTGGCAAATGaa                                                                                                                   <  1:411186/151‑1 (MQ=255)                                  cAGCCAAAATTATCGAGCAGCCACAGCTTAGCGTGGATGCTGTCGCCAACACCCTGGCCGGGTGGTCGCGAGAAACCTTATTAACCATGGCAGAACGCGCCCGCGCTGCATCCATGCCGGATGCCACCGAGCGAGTGGCAAATGAAGTg                                                                                                                >  2:230497/1‑149 (MQ=255)                                    gCCAAAATTATCGAGCAGCCACAGCTTAGCGTGGATGCTGTCGCCAACACCCTGGCCGGGTGGTCGCGAGAAACCTTATTAACCATGGCAGAACGCGCCCGCGCTGCATCCATGCCGGATGCCACCGAGCGAGTGGCAAATGAAGTGAGcc                                                                                                            <  1:843787/151‑1 (MQ=255)                                     ccAAAATTATCGAGCAGCCACAGCTTAGCGTGGATGCTGTCGCCAACACCCTGGCCGGGTGGTCGCGAGAAACCTTATTAACCATGGCAGAACGCGCCCGCGCTGCATCCATGCCGGATGCCACCGAGCGAGTGGCAAATGAAGTGAGCCg                                                                                                           <  2:755181/151‑1 (MQ=255)                                       aaaaTTATCGAGCAGCCACAGCTTAGCGTGGATGCTGTCGCCAACACCCTGGCCGGGTGGTCGCGAGAAACCTTATTAACCATGGCAGAACGCGCCCGCGCTGCATCCATGCCGGATGCCACCGAGCGAGTGGCAAATGAAGTGAGCCggg                                                                                                         >  2:352059/1‑151 (MQ=255)                                             aTCGAGCAGCCACAGCTTAGCGTGGATGCTGTCGCCAACACCCTGGCCGGGTGGTCGCGAGAAACCTTATTAACCATGGCAGAACGCGCCCGCGCTGCATCCATGCCGGATGCCACCGAGCGAGTGGCAAATGAAGTGAGCCGGGTTGccc                                                                                                   >  1:151823/1‑151 (MQ=255)                                                   cagcCACAGCTTAGCGTGGATGCTGTCGCCAACACCCTGGCCGGGTGGTCGCGAGAAACCTTATTAACCATGGCAGAACGCGCCCGCGCTGCATCCATGCCGGATGCCACCGAGCGAGTGGCAAATGAAGTGAGCCGGGTTGCCCGGGCGt                                                                                             <  2:489075/151‑1 (MQ=255)                                                    agcCACAGCTTAGCGTGGATGCTGTCGCCAACACCCTGGCCGGGTGGTCGCGAGAAACCTTATTAACCATGGCAGAACGCGCCCGCGCTGCATCCATGCCGGATGCCACCGAGCGAGTGGCAAATGAAGTGAGCCGGGTTGCCCGGGCGt                                                                                             >  1:562854/1‑150 (MQ=255)                                                      ccACAGCTTAGCGTGGATGCTGTCGCCAACACCCTGGCCGGGTGGTCGCGAGAAACCTTATTAACCATGGCAGAACGCGCCCGCGCTGCATCCATGCCGGATGCCACCGAGCGAGTGGCAAATGAAGTGAGCCGGGTTGCCCGGGCGTAAt                                                                                          <  2:714701/151‑1 (MQ=255)                                                       cacaGCTTAGCGTGGATGCTGTCGCCAACACCCTGGCCGGGTGGTCGCGAGAAACCTTATTAACCATGGCAGAACGCGCCCGCGCTGCATCCATGCCGGATGCCACCGAGCGAGTGGCAAATGAAGTGAGCCGGGTTGCCCGGGCGTAAtt                                                                                         <  1:89897/151‑1 (MQ=255)                                                           gCTTAGCGTGGATGCTGTCGCCAACACCCTGGCCGGGTGGTCGCGAGAAACCTTATTAACCATGGCAGAACGCGCCCGCGCTGCATCCATGCCGGATGCCACCGAGCGAGTGGCAAATGAAGTGAGCCGGGTTGCCCGGGCGTAATTGTAg                                                                                     <  1:313677/151‑1 (MQ=255)                                                             ttAGCGTGGATGCTGTCGCCAACACCCTGGCCGGGTGGTCGCGAGAAACCTTATTAACCATGGCAGAACGCGCCCGCGCTGCATCCATGCCGGATGCCACCGAGCGAGTGGCAAATGAAGTGAGCCGGGTTGACCGGGCGTAATTGTAGCg                                                                                   >  2:216380/1‑151 (MQ=255)                                                                    ggATGCTGTCGCCAACACCCTGGCCGGGTGGTCGCGAGAAACCTTATTAACCATGGCAGAACGCGCCCGCGCTGCATCCATGCCGGATGCCACCGAGCGAGTGGCAAATGAAGTGAGCCGGGTTGCCCGGGCGTAATTGTAGCGATGCCtt                                                                            >  2:540652/1‑151 (MQ=255)                                                                    ggATGCTGTCGCCAACACCCTGGCCGGGTGGTCGCGAGAAACCTTATTAACCATGGCAGAACGCGCCCGCGCTGCATCCATGCCGGATGCCACCGAGCGAGTGGCAAATGAAGTGAGCCGGGTTGCCCGGGCGTAATTGTAGCGATGCCtt                                                                            <  1:7916/151‑1 (MQ=255)                                                                    ggATGCTGTCGCCAACACCCTGGCCGGGTGGTCGCGAGAAACCTTATTAACCATGGCAGAACGCGCCCGCGCTGCATCCATGCCGGATGCCACCGAGCGAGTGGCAAATGAAGTGAGCCGGGTTGCCCGGGCGTAATTGTAGCGATGCCtt                                                                            <  1:166601/151‑1 (MQ=255)                                                                     gATGCTGTCGCCAACACCCTGGCCGGGTGGTCGCGAGAAACCTTATTAACCATGGCAGAACGCGCCCGCGCTGCATCCATGCCGGATGCCACCGAGCGAGTGGCAAATGAAGTGAGCCGGGTTGCCCGGGCGTAATTGTAGCGATGCCttt                                                                           <  2:562508/151‑1 (MQ=255)                                                                        gCTGTCGCCAACACCCTGGCCGGGTGGTCGCGAGAAACCTTATTAACCATGGCAGAACGCGCCCGCGCTGCATCCATGCCGGATGCCACCGAGCGAGTGGCAAATGAAGTGAGCCGGGTTGCCCGGGCGTAATTGTAGCGATGCCTTTTGc                                                                        >  1:686149/1‑151 (MQ=255)                                                                          tGTCGCCAACACCCTGGCCGGGTGGTCGCGAGAAACCTTATTAACCATGGCAGAACGCGCCCGCGCTGCATCCATGCCGGATGCCACCGAGCGAGTGGCAAATGAAGTGAGCCGGGTTGCCCGGGCGTAATTGTAGCGATGCCTTTTGCAt                                                                      >  2:44720/1‑151 (MQ=255)                                                                              gCCAACACCCTGGCCGGGTGGTCGCGAGAAACCTTATTAACCATGGCAGAACGCGCCCGCGCTGCATCCATGCCGGATGCCACCGAGCGAGTGGCAAATGAAGTGAGCCGGGTTGCCCGGGCGTAATTGTAGCGATGCCTTTTGCATCGt                                                                   >  2:784834/1‑150 (MQ=255)                                                                              gCCAACACCCTGGCCGGGTGGTCGCGAGAAACCTTATTAACCATGGCAGAACGCGCCCGCGCTGCATCCATGCCGGATGCCACCGAGCGAGTGGCAAATGAAGTGAGCCGGGTTGCCCGGGCGTAATTGTAGCGATGCCTTTTGCATCGTa                                                                  <  1:1088272/151‑1 (MQ=255)                                                                                    acCCTGGCCGGGTGGTCGCGAGAAACCTTATTAACCATGGCAGAACGCGCCCGCGCTGCATCCATGCCGGATGCCACCGAGCGAGTGGCAAATGAAGTGAGCCGGGTTGCCCGGGCGTAATTGTAGCGATGCCTTTTGCATCGTATGAAtt                                                            >  2:758878/1‑151 (MQ=255)                                                                                     cccTGGCCGGGTGGTCGCGAGAAACCTTATTAACCATGGCAGAACGCGCCCGCGCTGCATCCATGCCGGATGCCACCGAGCGAGTGGCAAATGAAGTGAGCCGGGTTGCCCGGGCGTAATTGTAGCGATGCCTTTTGCATCGTATGAAttt                                                           <  2:867506/151‑1 (MQ=255)                                                                                     cccTGGCCGGGTGGTCGCGAGAAACCTTATTAACCATGGCAGAACGCGCCCGCGCTGCATCCATGCCGGATGCCACCGAGCGAGTGGCAAATGAAGTGAGCCGGGTTGCCCGGGCGTAATTGTAGCGATGCCTTTTGCATCGTATGAAttt                                                           <  2:106127/151‑1 (MQ=255)                                                                                        tGGCCGGGTGGTCGCGAGAAACCTTATTAACCATGGCAGAACGCGCCCGCGCTGCATCCATGCCGGATGCCACCGAGCGAGTAGCAAATGAAGTGAGCCGGGTTGCCCGGGCGTAATTGTAGCGATGCCTTTTGCATCGTATGAATTTa                                                          >  2:128454/1‑149 (MQ=255)                                                                                         ggCCGGGTGGTCGCGAGAAACCTTATTAACCATGGCAGAACGCGCCCGCGCTGCATCCATGCCGGATGCCACCGAGCGAGTGGCAAATGAAGTGAGCCGGGTTGCCCGGGCGTAATTGTAGCGATGCCTTTTGCATCGTATGAATTTaag                                                        <  1:615993/150‑1 (MQ=255)                                                                                            cGGGTGGTCGCGAGAAACCTTATTAACCATGGCAGAACGCGCCCGCGCTGCATCCATGCCGGATGCCACCGAGCGAGTGGCAAATGAAGTGAGCCGGGTTGCCCGGGCGTAATTGTAGCGATGCCTTTTGCATCGTATGAATTTAAGAAGt                                                    <  2:96005/151‑1 (MQ=255)                                                                                            cGGGTGGTCGCGAGAAACCTTATTAACCATGGCAGAACGCGCCCGCGCTGCATCCATGCCGGATGCCACCGAGCGAGTGGCAAATGAAGTGAGCCGGGTTGCCCGGGCGTAATTGTAGCGATGCCTTTTGCATCGTATGAATTTAAGAAGt                                                    >  1:326865/1‑151 (MQ=255)                                                                                             gggTGGTCGCGAGAAACCTTATTAACCATGGCAGAACGCGCCCGCGCTGCATCCATGCCGGATGCCACCGAGCGAGTGGCAAATGAAGTGAGCCGGGTTGCCCGGGCGTAATTGTAGCGATGCCTTTTGCATCGTATGAATTTAAGAAGtt                                                   <  2:1085459/151‑1 (MQ=255)                                                                                               gtggtCGCGAGAAACCTTATTAACCATGGCAGAACGCGCCCGCGCTGCATCCATGCCGGATGCCACCGAGCGAGTGGCAAATGAAGTGAGCCGGGTTGCCCGGGCGTAATTGTAGCGATGCCTTTTGCATCGTATGAATTTAAGAAGTTaa                                                 <  1:800640/151‑1 (MQ=255)                                                                                                 ggtCGCGAGAAACCTTATTAACCATGGCAGAACGCGCCCGCGCTGCATCCATGCCGGATGCCACCGAGCGAGTGGCAAATGAAGTGAGCCGGGTTGCCCGGGCGTAATTGTAGCGATGCCTTTTGCATCGTATGAATTTAAGAAGTTAATg                                               >  1:1067477/1‑151 (MQ=255)                                                                                                 ggtCGCGAGAAACCTTATTAACCATGGCAGAACGCGCCCGCGCTGCATCCATGCCGGATGCCACCGAGCGAGTGGCAAATGAAGTGAGCCGGGTTGCCCGGGCGTAATTGTAGCGATGCCTTTTGCATCGTATGAATTTAAGAAGTTAATg                                               <  1:725421/151‑1 (MQ=255)                                                                                                  gtCGCGAGAAACCTTATTAACCATGGCAGAACGCGCCCGCGCTGCATCCATGCCGGATGCCACCGAGCGAGTGGCAAATGAAGTGAGCCGGGTTGCCCGGGCGTAATTGTAGCGATGCCTTTTGCATCGTATGAATTTAAGAAGTTAATgg                                              <  1:366210/151‑1 (MQ=255)                                                                                                  gtCGCGAGAAACCTTATTAACCATGGCAGAACGCGCCCGCGCTGCATCCATGCCGGATGCCACCGAGCGAGTGGCAAATGAAGTGAGCCGGGTTGCCCGGGCGTAATTGTAGCGATGCCTTTTGCATCGTATGAATTTAAGAAGTTAATgg                                              <  1:608525/151‑1 (MQ=255)                                                                                                  gtCGCGAGAAACCTTATTAACCATGGCAGAACGCGCCCGCGCTGCATCCATGCCGGATGCCACCGAGCGAGTGGCAAATGAAGTGAGCCGGGTTGCCCGGGCGTAATTGTAGCGATGCCTTTTGCATCGTATGAATTTAAGAAGTTAATgg                                              >  1:481913/1‑151 (MQ=255)                                                                                                     gcgAGAAACCTTATTAACCATGGCAGAACGCGCCCGCGCTGCATCCATGCCGGATGCCACCGAGCGAGTGGCAAATGAAGTGAGCCGGGTTGCCCGGGCGTAATTGTAGCGATGCCTTTTGCATCGTATGAATTTAAGAAGTTAATGGCGt                                           <  1:462698/151‑1 (MQ=255)                                                                                                      cgAGAAACCTTATTAACCATGGCAGAACGCGCCCGCGCTGCATCCATGCCGGATGCCACCGAGCGAGTGGCAAATGAAGTGAGCCGGGTTGCCCGGGCGTAATTGTAGCGATGCCTTTTGCATCGTATGAATTTAAGAAGTTAATGGCGt                                           >  2:943026/1‑150 (MQ=255)                                                                                                         gaAACCTTATTAACCATGGCAGAACGCGCCCGCGCTGCATCCATGCCGGATGCCACCGAGCGAGTGGCAAATGAAGTGAGCCGGGTTGCCCGGGCGTAATTGTAGCGATGCCTTTTGCATCGTATGAATTTAAGAAGTTAATGGCGTaa                                         >  1:526968/1‑149 (MQ=255)                                                                                                            aCCTTATTAACCATGGCAGAACGCGCCCGCGCTGCATCCATGCCGGATGACACCGAGCGAGTGGCAAATGAAGTGAGCCGGGTTGCCCGGGCGTAATTGTAGCGATGCCTTTTGCATCGTATGAATTTAAGAAGTTAATGGCGTAAAgaat                                    >  2:268209/1‑151 (MQ=255)                                                                                                             ccTTATTAACCATGGCAGAACGCGCCCGCGCTGCATCCATGCCGGATGCCACCGAGCGAGTGGCAAATGAAGTGAGCCGGGTTGCCCGGGCGTAATTGTAGCGATGCCTTTTGCATCGTATGAATTTAAGAAGTTAATGGCGTAAAgaatg                                   <  2:984060/151‑1 (MQ=255)                                                                                                             ccTTATTAACCATGGCAGAACGCGCCCGCGCTGCATCCATGCCGGATGCCACCGAGCGAGTGGCAAATGAAGTGAGCCGGGTTGCCCGGGCGTAATTGTAGCGATGCCTTTTGCATCGTATGAATTTAAGAAGTTAATGGCGTAAAgaat                                    >  2:365643/1‑150 (MQ=255)                                                                                                                tattaACCATGGCAGAACGCGCCCGCGCTGCATCCATGCCGGATGCCACCGAGCGAGTGGCAAATGAAGTGAGCCGGGTTGCCCGGGCGTAATTGTAGCGATGCCTTTTGCATCGTATGAATTTAAGAAGTTAATGGCGTAAAgaatga                                  <  2:239100/149‑1 (MQ=255)                                                                                                                   taACCATGGCAGAACGCGCCCGCGCTGCATCCATGCCGGATGCCACCGAGCGAGTGGCAAATGAAGTGAGCCGGGTTGCCCGGGCGTAATTGTAGCGATGCCTTTTGCATCGTATGAATTTAAGAAGTTAATGGCGTAAAGAATGAATac                              <  2:63468/150‑1 (MQ=255)                                                                                                                    aaCCATGGCAGAACGCGCCCGCGCTGCATCCATGCCGGATGCCACCGAGCGAGTGGCAAATGAAGTGAGCCGGGTTGCCCGGGCGTAATTGTAGCGATGCCTTTTGCATCGTATGAATTTAAGAAGTTAATGGCGTAAAGAATGAATaca                             <  2:690810/150‑1 (MQ=255)                                                                                                                    aaCCATGGCAGAACGCGCCCGCGCTGCATCCATGCCGGATGCCACCGAGCGAGTGGCAAATGAAGTGAGCCGGGTTGCCCGGGCGTAATTGTAGCGATGCCTTTTGCATCGTATGAATTTAAGAAGTTAATGGCGTAAAGAATGAATaca                             <  2:598714/150‑1 (MQ=255)                                                                                                                    aaCCATGGCAGAACGCGCCCGCGCTGCATCCATGCCGGATGCCACCGAGCGAGTGGCAAATGAAGTGAGCCGGGTTGCCCGGGCGTAATTGTAGCGATGCCTTTTGCATCGTATGAATTTAAGAAGTTAATGGCGTAAAGAATGAATaca                             <  1:186935/150‑1 (MQ=255)                                                                                                                     aCCATGGCAGAACGCGCCCGCGCTGCATCCATGCCGGATGCCACCGAGCGAGTGGCAAATGAAGTGAGCCGGGTTGCCCGGGCGTAATTGTAGCGATGCCTTTTGCATCGTATGAATTTAAGAAGTTAATGGCGTAAAGAATGAATacac                            >  1:1132080/1‑150 (MQ=255)                                                                                                                     aCCATGGCAGAACGCGCCCGCGCTGCATCCATGCCGGATGCCACCGAGCGAGTGGCAAATGAAGTGAGCCGGGTTGCCCGGGCGTAATTGTAGCGATGCCTTTTGCATCGTATGAATTTAAGAAGTTAATGGCGTAAAGAATGAATAcaca                           <  2:1070827/151‑1 (MQ=255)                                                                                                                        aTGGCAGAACGCGCCCGCGCTGCATCCATGCCGGATGCCACCGAGCGAGTGGCAAATGAAGTGAGCCGGGTTGCCCGGGCGTAATTGTAGCGATGCCTTTTGCATCGTATGAATTTAAGAAGTTAATGGCGTAAAGAATGAATACacaaca                        <  2:673130/151‑1 (MQ=255)                                                                                                                           gCAGAACGCGCCCGCGCTGCATCCATGCCGGATGCCACCGAGCGAGTGGCAAATGAAGTGAGCCGGGTTGCCCGGGCGTAATTGTAGCGATGCCTTTTGCATCGTATGAATTTAAGAAGTTAATGGCGTAAAGAATGAATACACAACAAtt                     <  1:873608/151‑1 (MQ=255)                                                                                                                           gCAGAACGCGCCCGCGCCGCATCCATGCCGGATGCCACCGAGCGAGTGGCAAATGAAGTGAGCCGGGTTGCCCGGGCGTAATTGTAGCGATGCCTTTTGCATCGTATGAATTTAAGAAGTTAATGGCGTAAAGAATGAATACACAACAAtt                     <  1:311228/151‑1 (MQ=255)                                                                                                                                 cgcgCCCGCGCTGCATCCATGCCGGATGCCACCGAGCGAGTGGCAAATGAAGTGAGCCGGGTTGCCCGGGCGTAATTGTAGCGATGCCTTTTGCATCGTATGAATTTAAGAAGTTAATGGCGTAAAGAATGAATACACAACAATTGGCaaa               <  1:114762/151‑1 (MQ=255)                                                                                                                                        gcgcTGCATCCATGCCGGATGCCACCGAGCGAGTGGCAAATGAAGTGAGCCGGGTTGCCCGGGCGTAATTGTAGCGATGCCTTTTGCATCGTATGAATTTAAGAAGTTAATGGCGTAAAGAATGAATACACAACAATTGGCAAAACTGCGt        >  1:684265/1‑151 (MQ=255)                                                                                                                                        gcgcTGCATCCATGCCGGATGCCACCGAGCGAGTGGCAAATGAAGTGAGCCGGGTTGCCCGGGCGTAATTGTAGCGATGCCTTTTGCATCGTATGAATTTAAGAAGTTAATGGCGTAAAGAATGAATACACAACAATTGGCAAAACTGCGt        <  2:942288/151‑1 (MQ=255)                                                                                                                                          gcTGCATCCATGCCGGATGCCACCGAGCGAGTGGCAAATGAAGTGAGCCGGGTTGCCCGGGCGTAATTGTAGCGATGCCTTTTGCATCGTATGAATTTAAGAAGTTAATGGCGTAAAGAATGAATACACAACAATTGGCAAAACTGCGTTc      >  1:594061/1‑151 (MQ=255)                                                                                                                                            tGCATCCATGCCGGATGCCACCGAGCGAGTGGCAAATGAAGTGAGCCGGGTTGCCCGGGCGTAATTGTAGCGATGCCTTTTGCATCGTATGAATTTAAGAAGTTAATGGCGTAAAGAATGAATACACAACAATTGGCAAAACTGCGTTcc     >  2:405227/1‑150 (MQ=255)                                                                                                                                              cATCCATGCCGGATGCCACCGAGCGAGTGGCAAATGAAGTGAGCCGGGTTGCCCGGGCGTAATTGTAGCGATGCCTTTTGCATCGTATGAATTTAAGAAGTTAATGGCGTAAAGAATGAATACACAACAATTGGCAAAACTGCGTTCCATc  <  2:417874/151‑1 (MQ=255)                                                                                                                                              cATCCATGCCGGATGCCACCGAGCGAGTGGCAAATGAAGTGAGCCGGGTTGCCCGGGCGTAATTGTAGCGATGCCTTTTGCATCGTATGAATTTAAGAAGTTAATGGCGTAAAGAATGAATACACAACAATTGGCAAAACTGCGTTCCATc  <  2:277469/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                ACTGGAATGCGCTACCGCTGGAAAAAGCGGGCGCAGCCAAAATTATCGAGCAGCCACAGCTTAGCGTGGATGCTGTCGCCAACACCCTGGCCGGGTGGTCGCGAGAAACCTTATTAACCATGGCAGAACGCGCCCGCGCTGCATCCATTCCGGATGCCACCGAGCGAGTGGCAAATGAAGTGAGCCGGGTTGCCCGGGCGTAATTGTAGCGATGCCTTTTGCATCGTATGAATTTAAGAAGTTAATGGCGTAAAGAATGAATACACAACAATTGGCAAAACTGCGTTCCATC  >  NC_000913/100509‑100800

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑