Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,591,492	A→C	L82F (TTA→TTC)	yffB →	putative ArsC family reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,591,492	0	A	C	100.0%	219.8 / NA	54	L82F (TTA→TTC)	yffB	putative ArsC family reductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (20/34);  total (20/34)

CGTCGATGGGCTGGACAGCGAATTATTGAACGATTTTATCAACGAATTAGGCTGGGAAGCGTTACTCAACACCCGTGGTACAACCTGGCGTAAACTGGACGAAACCACCCGCAATAAAATCACCGATGCGGCCTCTGCGGCGGCATTAATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACACAACAG  >  NC_000913/2591345‑2591639                                                                                                                                                    |                                                                                                                                                    cgTCGATGGGCTGGACAGCGAATTATTGAACGATTTTATCAACGAATTAGGCTGGGAAGCGTTACTCAACACCCGTGGTACAACCTGGCGTAAACTGGACGAAACCACCCGCAATAAAATCACCGATGCGGCCTCTGCGGCGGCATTCATg                                                                                                                                                  <  2:891869/151‑1 (MQ=255) cgTCGATGGGCTGGACAGCGAATTATTGAACGATTTTATCAACGAATTAGGCTGGGAAGCGTTACTCAACACCCGTGGTACAACCTGGCGTAAACTGGACGAAACCACCCGCAATAAAATCACCGATGCGGCCTCTGCGGCGGCATTCATg                                                                                                                                                  <  1:478737/151‑1 (MQ=255)    cGATGGGCTGGACAGCGAATTATTGAACGATTTTATCAACGAATTAGGCTGGGAAGCGTTACTCAACACCCGTGGTACAACCTGGCGTAAACTGGACGAAACCACCCGCAATAAAATCACCGATGCGGCCTCTGCGGCGGCATTCATGACt                                                                                                                                               <  2:301869/151‑1 (MQ=255)            tGGACAGCGAATTATTGAACGATTTTATCAACGAATTAGGCTGGGAAGCGTTACTCAACACCCGTGGTACAACCTGGCGTAAACTGGACGAAACCACCCGCAATAAAATCACCGATGCGGCCTCTGCGGCGGCATTCATGACTGAAATGcc                                                                                                                                       >  2:600274/1‑151 (MQ=255)             ggACAGCGAATTATTGAACGATTTTATCAACGAATTAGGCTGGGAAGCGTTACTCAACACCCGTGGTACAACCTGGCGTAAACTGGACGAAACCACCCGCAATAAAATCACCGATGCGGCCTCTGCGGCGGCATTCATGACTGAAATGCCt                                                                                                                                      <  1:490614/151‑1 (MQ=255)               aCAGCGAATTATTGAACGATTTTATCAACGAATTAGGCTGGGAAGCGTTACTCAACACCCGTGGTACAACCTGGCGTAAACTGGACGAAACCACCCGCAATAAAATCACCGATGCGGCCTCTGCGGCGGCATTCATGACTGAAATGCCTGc                                                                                                                                    >  1:691423/1‑151 (MQ=255)                 aGCGAATTATTGAACGATTTTATCAACGAATTAGGCTGGGAAGCGTTACTCAACACCCGTGGTACAACCTGGCGTAAACTGGACGAAACCACCCGCAATAAAATCACCGATGCGGCCTCTGCGGCGGCATTCATGACTGAAATGCCTGCa                                                                                                                                   >  1:252041/1‑150 (MQ=255)                   cGAATTATTGAACGATTTTATCAACGAATTAGGCTGGGAAGCGTTACTCAACACCCGTGGTACAACCTGGCGTAAACTGGACGAAACCACCCGCAATAAAATCACCGATGCGGCCTCTGCGGCGGCATTCATGACTGAAATGCCTGCAAtt                                                                                                                                >  2:976284/1‑151 (MQ=255)                   cGAATTATTGAACGATTTTATCAACGAATTAGGCTGGGAAGCGTTACTCAACACCCGTGGTACAACCTGGCGTAAACTGGACGAAACCACCCGCAATAAAATCACCGATGCGGCCTCTGCGGCGGCATTCATGACTGAAATGCCTGCAAtt                                                                                                                                <  2:1034065/151‑1 (MQ=255)                    gAATTATTGAACGATTTTATCAACGAATTAGGCTGGGAAGCGTTACTCAACACCCGTGGTACAACCTGGCGTAAACTGGACGAAACCACCCGCAATAAAATCACCGATGCGGCCTCTGCGGCGGCATTCATGACTGAAATGCCTGCAATTa                                                                                                                               <  1:55402/151‑1 (MQ=255)                     aaTTATTGAACGATTTTATCAACGAATTAGGCTGGGAAGCGTTACTCAACACCCGTGGTACAACCTGGCGTAAACTGGACGAAACCACCCGCAATAAAATCACCGATGCGGCCTCTGCGGCGGCATTCATGACTGAAATGCCTGCAATTa                                                                                                                               >  1:1116713/1‑150 (MQ=255)                      attattGAACGATTTTATCAACGAATTAGGCTGGGAAGCGTTACTCAACACCCGTGGTACAACCTGGCGTAAACTGGACGAAACCACCCGCAATAAAATCACCGATGCGGCCTCTGCGGCGGCATTCATGACTGAAATGCCTGCAATTATc                                                                                                                             >  1:43981/1‑151 (MQ=255)                        tattGAACGATTTTATCAACGAATTAGGCTGGGAAGCGTTACTCAACACCCGTGGTACAACCTGGCGTAAACTGGACGAAACCACCCGCAATAAAATCACCGATGCGGCCTCTGCGGCGGCATTCATGACTGAAATGCCTGCAATTATCa                                                                                                                            <  2:830715/150‑1 (MQ=255)                           tGAACGATTTTATCAACGAATTAGGCTGGGAAGCGTTACTCAACACCCGTGGTACAACCTGGCGTAAACTGGACGAAACCACCCGCAATAAAATCACCGATGCGGCCTCTGCGGCGGCATTCATGACTGAAATGCCTGCAATTATCAAAc                                                                                                                         <  1:170275/150‑1 (MQ=255)                             aaCGATTTTATCAACGAATTAGGCTGGGAAGCGTTACTCAACACCCGTGGTACAACCTGGCGTAAACTGGACGAAACCACCCGCAATAAAATCACCGATGCGGCCTCTGCGGCGGCATTCATGACTGAAATGCCTGCAATTATCAAACGTc                                                                                                                      <  2:160010/151‑1 (MQ=255)                               cGATTTTATCAACGAATTAGGCTGGGAAGCGTTACTCAACACCCGTGGTACAACCTGGCGTAAACTGGACGAAACCACCCGCAATAAAATCACCGATGCGGCCTCTGCGGCGGCATTCATGACTGAAATGCCTGCAATTATCAAACGTCCa                                                                                                                    <  2:785034/151‑1 (MQ=255)                                   tttATCAACGAATTAGGCTGGGAAGCGTTACTCAACACCCGTGGTACAACCTGGCGTAAACTGGACGAAACCACCCGCAATAAAATCACCGATGCGGCCTCTGCGGCGGCATTCATGACTGAAATGCCTGCAATTATCAAACGTCCATTg                                                                                                                 <  2:653844/150‑1 (MQ=255)                                    ttATCAACGAATTAGGCTGGGAAGCGTTACTCAACACCCGTGGTACAGCCTGGCGTAAACTGGACGAATCCACCCGCAATAAAATCACCGATGAGGACTCTGCGGCGGCATTCATGACTGAAATGCCTGCAATTATCAAACGTCCAtt                                                                                                                  <  2:746554/148‑1 (MQ=255)                                     tATCAACGAATTAGGCTGGGAAGCGTTACTCAACACCCGTGGTACAACCTGGCGTAAACTGGACGAAACCACCCGCAATAAAATCACCGATGCGGCCTCTGCGGCGGCATTCATGACTGAAATGCCTGCAATTATCAAACGTCCATTGct                                                                                                               <  2:744767/150‑1 (MQ=255)                                      aTCAACGAATTAGGCTGGGAAGCGTTACTCAACACCCGTGGTACAACCTGGCGTAAACTGGACGAAACCACCCGCAATAAAATCACCGATGCGGCCTCTGCGGCGGCATTCATGACTGAAATGCCTGCAATTATCAAACGTCCATTGctct                                                                                                             <  1:659649/151‑1 (MQ=255)                                       tCAACGAATTAGGCTGGGAAGCGTTACTCAACACCCGTGGTACAACCTGGCGTAAACTGGACGAAACCACCCGCAATAAAATCACCGATGCGGCCTCTGCGGCGGCATTCATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTg                                                                                                            >  1:463640/1‑151 (MQ=255)                                           cGAATTAGGCTGGGAAGCGTTACTCAACACCCGTGGTACAACCTGGCGTAAACTGGACGAAACCACCCGCAATAAAATCACCGATGCGGCCTCTGCGGCGGCATTCATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTg                                                                                                        <  1:529311/151‑1 (MQ=255)                                             aaTTAGGCTGGGAAGCGTTACTCAACACCCGTGGTACAACCTGGCGTAAACTGGACGAAACCACCCGCAATAAAATCACCGATGCGGCCTCTGCGGCGGCATTCATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGcc                                                                                                      >  2:216806/1‑151 (MQ=255)                                                tAGGCTGGGAAGCGTTACTCAACACCCGTGGTACAACCTGGCGTAAACTGGACGAAACCACCCGCAATAAAATCACCGATGCGGCCTCTGCGGCGGCATTCATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCgg                                                                                                   >  1:1077259/1‑151 (MQ=255)                                                tAGGCTGGGAAGCGTTACTCAACACCCGTGGTACAACCTGGCGTAAACTGGACGAAACCACCCGCAATAAAATCACCGATGCGGCCTCTGCGGCGGCATTCATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCg                                                                                                    <  2:630746/150‑1 (MQ=255)                                                      gggAAGCGTTACTCAACACCCGTGGTACAACCTGGCGTAAACTGGACGAAACCACCCGCAATAAAATCACCGATGCGGCCTCTGCGGCGGCATTCATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAg                                                                                               <  2:686505/149‑1 (MQ=255)                                                            cGTTACTCAACACCCGTGGTACAACCTGGCGTAAACTGGACGAAACCACCCGCAATAAAATCACCGATGCGGCCTCTGCGGCGGCATTCATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTAtgct                                                                                       >  1:719382/1‑151 (MQ=255)                                                            cGTTACTCAACACCCGTGGTACAACCTGGCGTAAACTGGACGAAACCACCCGCAATAAAATCACCGATGCGGCCTCTGCGGCGGCATTCATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTAtgct                                                                                       <  2:776154/151‑1 (MQ=255)                                                             gTTACTCAACACCCGTGGTACAACCTGGCGTAAACTGGACGAAACCACCCGCAATAAAATCACCGATGCGGCCTCTGCGGCGGCATTCATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTAtgctg                                                                                      <  2:793510/151‑1 (MQ=255)                                                                aCTCAACACCCGTGGTACAACCTGGCGTAAACTGGACGAAACCACCCGCAATAAAATCACCGATGCGGCCTCTGCGGCGGCATTCATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATgctgct                                                                                    <  2:223159/150‑1 (MQ=255)                                                                             ggTACAACCTGGCGTAAACTGGACGAAACCACCCGCAATAAAATCACCGATGCGGCCTCTGCGGCGGCATTCATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGAtt                                                                      >  1:294325/1‑151 (MQ=255)                                                                                aCAACCTGGCGTAAACTGGACGAAACCACCCGCAATAAAATCACCGATGCGGCCTCTGCGGCGGCATTCATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCa                                                                   <  1:50460/151‑1 (MQ=255)                                                                                   aCCTGGCGTAAACTGGACGAAACCACCCGCAATAAAATCACCGATGCGGCCTCTGCGGCGGCATTCATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGtt                                                                <  2:537723/151‑1 (MQ=255)                                                                                            aaaCTGGACGAAACCACCCGCAATAAAATCACCGATGCGGCCTCTGCGGCGGCATTCATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAAt                                                       <  2:778418/151‑1 (MQ=255)                                                                                                      aaaCCACCCGCAATAAAATCACCGATGCGGCCTCTGCGGCGGCATTCATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGa                                             <  1:1002085/151‑1 (MQ=255)                                                                                                              cGCAATAAAATCACCGATGCGGCCTCTGCGGCGGCATTCATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGGTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGt                                     <  2:996248/151‑1 (MQ=255)                                                                                                                 aaTAAAATCACCGATGCGGCCTCTGCGGCGGCATTCATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCt                                   >  1:869682/1‑150 (MQ=255)                                                                                                                  aTAAAATCACCGATGCGGCCTCTGCGGCGGCATTCATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTAt                                 <  1:791090/151‑1 (MQ=255)                                                                                                                    aaaaTCACCGATGCGGCCTCTGCGGCGGCATTCATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATg                                <  2:29787/150‑1 (MQ=255)                                                                                                                     aaaTCACCGATGCGGCCTCTGCGGCGGCATTCATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTc                              <  2:55838/151‑1 (MQ=255)                                                                                                                         cACCGATGCGGCCTCTGCGGCGGCATTCATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTg                           <  1:515389/150‑1 (MQ=255)                                                                                                                           ccGATGCGGCCTCTGCGGCGGCATTCATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGccc                        <  2:821341/151‑1 (MQ=255)                                                                                                                            cGATGCGGCCTCTGCGGCGGCATTCATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCg                       >  2:624429/1‑151 (MQ=255)                                                                                                                              aTGCGGCCTCTGCGGCGGCATTCATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCgg                      >  1:712971/1‑150 (MQ=255)                                                                                                                              aTGCGGCCTCTGCGGCGGCATTCATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGt                     >  2:544010/1‑151 (MQ=255)                                                                                                                                      tctGCGGCGGCATTCATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGc             >  2:621220/1‑151 (MQ=255)                                                                                                                                       ctGCGGCGGCATTCATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAg              <  1:516960/149‑1 (MQ=255)                                                                                                                                       ctGCGGCGGCATTCATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCt            >  2:124146/1‑151 (MQ=255)                                                                                                                                       ctGCGGCGGCATTCATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCt            >  1:934866/1‑151 (MQ=255)                                                                                                                                            gcggcATTCATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGacac       <  2:43975/151‑1 (MQ=255)                                                                                                                                            gcggcATTCATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGacac       >  1:1039102/1‑151 (MQ=255)                                                                                                                                              ggcATTCATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGAcaca      >  1:413113/1‑150 (MQ=255)                                                                                                                                                cATTCATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACacaaca   <  1:427538/151‑1 (MQ=255)                                                                                                                                                 aTTCATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACACAACAg  <  2:1073631/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                    CGTCGATGGGCTGGACAGCGAATTATTGAACGATTTTATCAACGAATTAGGCTGGGAAGCGTTACTCAACACCCGTGGTACAACCTGGCGTAAACTGGACGAAACCACCCGCAATAAAATCACCGATGCGGCCTCTGCGGCGGCATTAATGACTGAAATGCCTGCAATTATCAAACGTCCATTGCTCTGCGTGCCCGGTAAGCCTATGCTGCTGGGTTTCAGTGATTCCAGTTATCAGCAATTTTTCCATGAGGTGTAGTCTATGTCGTGCCCGGTTATTGAGCTGACACAACAG  >  NC_000913/2591345‑2591639

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑