Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	707,238	T→G	F382L (TTT→TTG)	glnS →	glutamyl‑tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	707,238	0	T	G	100.0%	299.7 / NA	84	F382L (TTT→TTG)	glnS	glutamyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (42/42);  total (42/42)

GAAGATCTCAACGAAAATGCGCCGCGCGCAATGGCGGTTATCGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTTAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGTGCGTCTGCGTAATGCTTATGTGATTAAGGCAGAACGCGTCGAGAAAGATGCCGAAGGTAATATC  >  NC_000913/707092‑707385                                                                                                                                                   |                                                                                                                                                    gAAGATCTCAACGAAAATGCGCCGCGCGCAATGGCGGTTATCGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTGAGc                                                                                                                                                  <  2:1002889/150‑1 (MQ=255) gAAGATCTCAACGAAAATGCGCCGCGCGCAATGGCGGTTATCGATCCGGGGAAAATGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAAACATCCGAACAAAACGGAAAAGGGCAGCCGTCAGGTGGCGTTGAGCg                                                                                                                                                 >  1:936385/1‑151 (MQ=255)  aaGATCTCAACGAAAATGCGCCGCGCGCAATGGCGGTTATCGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCATCCGTCAGGTGCCGTTGAGCg                                                                                                                                                 <  2:463130/150‑1 (MQ=255)   aGATCTCAACGAAAATGCGCCGCGCGCAATGGCGGTTATCGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGGAATGGGTACCATGCCGAACCATCCGAACAAACCGGAAAAGGGGAGCCGGCAGGGGCCGGTGAGCggg                                                                                                                                               >  2:368250/1‑150 (MQ=255)    gATCTCAACGAAAATGCGCCGCGCGCAATGGCGGTTATCGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGGAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAAACGGAAAAGGGCAGCCGTCAGGGGCCGTTGAGCGGTg                                                                                                                                              >  1:1049579/1‑151 (MQ=255)         cAACGAAAATGCGCCGCGCGCAATGGCGGTTATCGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTGAGCGGTgag                                                                                                                                            <  1:203072/148‑1 (MQ=255)         cAACGAAAATGCGCCGCGCGCAATGGCGGTTATCGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTGAGCGGTg                                                                                                                                              <  2:871147/146‑1 (MQ=255)         cAACGAAAATGCGCCGCGCGCAATGGCGGTTATCGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTGAGCGGTg                                                                                                                                              <  2:809439/146‑1 (MQ=255)           aCGAAAATGCGCCGCGCGCAATGGCGGTTATCGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTGAGCGGTGAGAttt                                                                                                                                        <  2:955828/150‑1 (MQ=255)           aCGAAAATGCGCCGCGCGCAATGGCGGTTATCGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTGAGCGGTGAGATTTg                                                                                                                                       <  1:1037881/151‑1 (MQ=255)           aCGAAAATGCGCCGCGCGCAATGGCGGTTATCGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTGAGCGGTGAGATTTg                                                                                                                                       <  1:1128361/151‑1 (MQ=255)               aaaTGCGCCGCGCGCAATGGCGGTTATCGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTGAGCGGTGAGATTTGGAtt                                                                                                                                   <  1:855379/151‑1 (MQ=255)               aaaTGCGCCGCGCGCAATGGCGGTTATCGATCCGGGGAAACTGGTTATCGAAAACTATCAGGGCGGAGGCGAAAAGGTTAACATGCCGAACCATCCCAACAAACCGGGAATGGGCAGCCGTCAGGGGCCGTTGAGCGGGGAGGTTTGGAtt                                                                                                                                   >  2:746879/1‑151 (MQ=255)               aaaTGCGCCGCGCGCAATGGCGGTTATCGATCCGGGGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGGTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGGGGCGTTGAGCGGGGAGATTTGGAtt                                                                                                                                   >  1:26941/1‑151 (MQ=255)                    cgccgcGCGCAATGGCGGTTATCGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTGAGCGGTGAGATTTGGATTGATcg                                                                                                                              >  1:332119/1‑151 (MQ=255)                     gccgcGCGCAATGGCGGTTATCGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTGAGCGGTGAGATTTGGATTGATcgc                                                                                                                             <  2:932282/151‑1 (MQ=255)                     gccgcGCGCAATGGCGGTTATCGATCCGGTGAAACTGGGTTTCGAAAACTATCAGGGGGGAGGCGAAAAGGGTACCCTGCCGAACCATCCGAACAAAACGGAAAAGGGCAGGCGTCAGGGGGCGTTGAGCGGTGAGATTTGGATTGGTcg                                                                                                                              >  2:25149/1‑150 (MQ=255)                        gcgcgcAATGGCGGTTATCGAACCGGTGGAACTGGTTATCGAAAACTATCAGGGCGAAGGCGGAAAGGGTACCATGCCGAACCATACGAACAAACCGGAAATGGGGAGCCGCCCGGTGCCGGTGAGCGGGGAGATTTGGGTTGATCGCGcc                                                                                                                          >  2:133849/1‑151 (MQ=255)                          gcgcAATGGCGGTTATTGATCCGGGGAAACTGGTTATTGAAAACTATCAGGGCGAAGGCGAAATGGGTACCATGCCGAACCATCCGAAAAAACCGGAAATGGGGAGCCGGCAGGGGCCGTTGAGCGGTGAGATTTGGATTGATTGCGCCg                                                                                                                         >  2:647846/1‑150 (MQ=255)                            gcAATGGCGGTTATCGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTGAGCGGTGAGATTTGGATTGATCGCGCCGAtt                                                                                                                      <  1:703746/151‑1 (MQ=255)                                tGGCGGTTATCGATCCGGTGAAACTGGTTATCGAAAAATATCAGGGGGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTGAGCGGTGAGATTTGGATTGATCGCGCCGATTTCcg                                                                                                                  >  2:366646/1‑151 (MQ=255)                                     gTTATCGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTGAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCgaag                                                                                                             <  2:143434/151‑1 (MQ=255)                                          cGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTGAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCt                                                                                                        >  1:474139/1‑151 (MQ=255)                                          cGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTGAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCt                                                                                                        >  2:329799/1‑151 (MQ=255)                                           gATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTGAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCt                                                                                                        >  2:244249/1‑150 (MQ=255)                                            aTCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTGAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTa                                                                                                       >  2:365553/1‑150 (MQ=255)                                            aTCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTGAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTa                                                                                                       >  2:776823/1‑150 (MQ=255)                                              ccGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTGAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACa                                                                                                    <  1:494716/151‑1 (MQ=255)                                              ccGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTGAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACa                                                                                                    <  1:767463/151‑1 (MQ=255)                                                ggTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTGAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACa                                                                                                    >  2:573552/1‑149 (MQ=255)                                                 gTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTGAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGc                                                                                                 <  1:41336/151‑1 (MQ=255)                                                    aaaCTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTGAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGt                                                                                              <  1:776931/151‑1 (MQ=255)                                                       cTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATAGGCAGCCGTCAGGTGCCGTTGAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTAc                                                                                            >  2:1102234/1‑150 (MQ=255)                                                         ggTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTGAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACaa                                                                                          >  1:969463/1‑150 (MQ=255)                                                             aTCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTGAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTc                                                                                     >  1:722475/1‑151 (MQ=255)                                                                gAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTGAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTgg                                                                                  >  2:963019/1‑151 (MQ=255)                                                                 aaaaCTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTGAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGt                                                                                 <  1:905263/151‑1 (MQ=255)                                                                 aaaaCTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTGAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGt                                                                                 <  2:233449/151‑1 (MQ=255)                                                                   aaCTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTGAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGc                                                                               <  1:408986/151‑1 (MQ=255)                                                                      tATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTGAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTgg                                                                            >  1:860334/1‑151 (MQ=255)                                                                       aTCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTGAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAGGCTAACAAGCAGTACAAACGTCTGGTGCTg                                                                             <  2:150113/149‑1 (MQ=255)                                                                       aTCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTGAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTgg                                                                            <  2:454179/150‑1 (MQ=255)                                                                       aTCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTGAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTg                                                                             <  1:840195/149‑1 (MQ=255)                                                                       aTCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTGAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTg                                                                             <  2:28630/149‑1 (MQ=255)                                                                            ggcgaaggcgaaATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTGAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTaa                                                                        >  1:231805/1‑149 (MQ=255)                                                                            ggcgaaggcgaaATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTGAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTaa                                                                        >  1:1084451/1‑149 (MQ=255)                                                                                aaggcgaaATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTGAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGt                                                                  <  1:351899/151‑1 (MQ=255)                                                                                    cgaaATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTGAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGTGCg                                                               >  1:118919/1‑150 (MQ=255)                                                                                      aaaTGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTGAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGTGCGTCt                                                            >  1:19393/1‑151 (MQ=255)                                                                                      aaaTGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTGAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGTGCGTCt                                                            <  1:562024/151‑1 (MQ=255)                                                                                       aaTGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTGAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGTGCGTCTg                                                           >  2:726804/1‑151 (MQ=255)                                                                                               ccATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTGAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGTGCGTCTGCGTAATg                                                    <  1:404966/150‑1 (MQ=255)                                                                                                 aTGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTGAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGTGCGTCTGCGTAATGCtt                                                 >  2:1108201/1‑151 (MQ=255)                                                                                                  tGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTGAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGTGCGTCTGCGTAATGCtt                                                 >  2:820329/1‑150 (MQ=255)                                                                                                     cGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTGAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGTGCGTCTGCGTAATGCTTAtgt                                             <  2:409196/151‑1 (MQ=255)                                                                                                      gAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTGAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGTGCGTCTGCGTAATGCTTAtgtg                                            >  2:781499/1‑151 (MQ=255)                                                                                                        aCCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTGAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGTGCGTCTGCGTAATGCTTATGTGAt                                          <  1:618488/151‑1 (MQ=255)                                                                                                        aCCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTGAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGTGCGTCTGCGTAATGCTTATGTGAt                                          <  1:461495/151‑1 (MQ=255)                                                                                                          cATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTGAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGGGAAGAA‑CTAACAAAGAGGAAAAACGGCTGGGGCTGGGGAAAGAAGGGCGGTTGGGGAAAGCTTaaggggtt                                         >  2:523948/1‑142 (MQ=255)                                                                                                            tCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTGAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGTGCGTCTGCGTAATGCTTATGTGATTa                                        >  1:186546/1‑149 (MQ=255)                                                                                                             ccGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTGAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGTGCGTCTGCGTAATGCTTATGTGATTAAg                                      <  2:966304/150‑1 (MQ=255)                                                                                                                aaCAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTGAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGTGCGTCTGCGTAATGCTTATGTGATTAAGGc                                    >  1:245088/1‑149 (MQ=255)                                                                                                                aaCAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTGAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGTGCGTCTGCGTAATGCTTATGTGATTAAGGCa                                   <  2:699572/150‑1 (MQ=255)                                                                                                                aaCAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTGAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGCGCTGGGTAAAGAAGTGCGTCTGCGTAATGCTTATGTGATTaa                                       >  2:146423/1‑146 (MQ=255)                                                                                                                 aCAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTGAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGTGCGTCTGCGTAATGCTTATGTGATTAAGGCAGa                                 <  1:776940/151‑1 (MQ=255)                                                                                                                      ccGGAAATGGGCAGCCGTCAGGTGCCGTTGAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGTGCGTCTGCGTAATGCTTATGTGATTAAGGCAGAAcgcg                            >  2:1112947/1‑151 (MQ=255)                                                                                                                       cGGAAATGGGCAGCCGTCAGGTGCCGTTGAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGTGCGTCTGCGTAATGCTTATGTGATTAAGGCAGAACGCGt                           >  2:267713/1‑151 (MQ=255)                                                                                                                        ggAAATGGGCAGCCGTCAGGTGCCGTTGAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGTGCGTCTGCGTAATGCTTATGTGATTAAGGCAGAACGCGt                           >  2:348699/1‑150 (MQ=255)                                                                                                                         gAAATGGGCAGCCGTCAGGTGCCGTTGAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGTGCGTCTGCGTAATGCTTATGTGATTAAGGCAGAACGCGTCg                         <  2:149296/151‑1 (MQ=255)                                                                                                                           aaTGGGCAGCCGTCAGGTGCCGTTGAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGTGCGTCTGCGTAATGCTTATGTGATTAAGGCAGAACGCGTCgag                       <  1:945574/151‑1 (MQ=255)                                                                                                                               ggCAGCCGTCAGGTGCCGTTGAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGTGCGTCTGCGTAATGCTTATGTGATTAAGGCAGAACGCGTCGAGaa                     <  1:2464/149‑1 (MQ=255)                                                                                                                                 cAGCCGTCAGGTGCCGTTGAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGTGCGTCTGCGTAATGCTTATGTGATTAAGGCAGAACGCGTCGAGaa                     >  1:388983/1‑147 (MQ=255)                                                                                                                                      gTCAGGTGCCGTTGAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGTGCGTCTGCGTAATGCTTATGTGATTAAGGCAGAACGCGTCGAGAAAGATGCCg             >  2:494498/1‑150 (MQ=255)                                                                                                                                      gTCAGGTGCCGTTGAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGTGCGTCTGCGTAATGCTTATGTGATTAAGGCAGAACGCGTCGAGAAAGATGCCGa            <  2:84597/151‑1 (MQ=255)                                                                                                                                        cAGGTGCCGTTGAGCGGTGAGATTTGGATTGATTGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGTGCGTCTGCGTAATGCTTATGTGATTAAGGCAGAACGCGTCGAGAAAGATGCCGa            >  2:24745/1‑149 (MQ=255)                                                                                                                                        cAGGTGCCGTTGAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGTGCGTCTGCGTAATGCTTATGTGATTAAGGCAGAACGCGTCGAGAAAGATGCCGa            >  1:674433/1‑149 (MQ=255)                                                                                                                                        cAGGTGCCGTTGAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGTGCGTCTGCGTAATGCTTATGTGATTAAGGCAGAACGCGTCGAGAAAGATGCCGAAg          <  1:1000539/151‑1 (MQ=255)                                                                                                                                         aGGTGCCGTTGAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGTGCGTCTGCGTAATGCTTATGTGATTAAGGCAGAACGCGTCGAGAAAGATGCCGAAgg         <  1:329849/151‑1 (MQ=255)                                                                                                                                          ggTGCCGTTGAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGTGCGTCTGCGTAATGCTTATGTGATTAAGGCAGAACGCGTCGAGAAAGATGCCGAAGGt        <  2:387969/151‑1 (MQ=255)                                                                                                                                             gCCGTTGAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGTGCGTCTGCGTAATGCTTATGTGATTAAGGCAGAACGCGTCGAGAAAGATGCCGAAGGTAat     <  2:813026/151‑1 (MQ=255)                                                                                                                                             gCCGTTGAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGTGCGTCTGCGTAATGCTTATGTGATTAAGGCAGAACGCGTCGAGAAAGATGCCGAAGGTAat     >  2:254706/1‑151 (MQ=255)                                                                                                                                              ccGTTGAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGTGCGTCTGCGTAATGCTTATGTGATTAAGGCAGAACGCGTCGAGAAAGATGCCGAAGGTAat     >  2:230915/1‑150 (MQ=255)                                                                                                                                                gTTGAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGTGCGTCTGCGTAATGCTTATGTGATTAAGGCAGAACGCGTCGAGAAAGATGCCGAAGGTAatat   <  2:807552/150‑1 (MQ=255)                                                                                                                                                gTTGAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGTGCGTCTGCGTAATGCTTATGTGATTAAGGCAGAACGCGTCGAGAAAGATGCCGAAGGTAATATc  <  2:860221/151‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                    GAAGATCTCAACGAAAATGCGCCGCGCGCAATGGCGGTTATCGATCCGGTGAAACTGGTTATCGAAAACTATCAGGGCGAAGGCGAAATGGTTACCATGCCGAACCATCCGAACAAACCGGAAATGGGCAGCCGTCAGGTGCCGTTTAGCGGTGAGATTTGGATTGATCGCGCCGATTTCCGCGAAGAAGCTAACAAGCAGTACAAACGTCTGGTGCTGGGTAAAGAAGTGCGTCTGCGTAATGCTTATGTGATTAAGGCAGAACGCGTCGAGAAAGATGCCGAAGGTAATATC  >  NC_000913/707092‑707385

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑