Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,206,435	0	C	T	44.9%	73.9 / 40.3	69	intergenic (‑179/‑28)	ttdR/ttdA	transcriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (8/30);  new base T (31/0);  total (39/30)
Fisher's exact test for biased strand distribution p-value = 1.65e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGA‑TGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTA  >  NC_000913/3206287‑3206582                                                                                                                                                       |                                                                                                                                                      gtgtATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTCCa                                                                                                                                                     <  1:698783/151‑1 (MQ=255)               aCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTCCAGACAAATACaaaaa                                                                                                                                       <  2:523561/151‑1 (MQ=255)                cATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTCCAGACAAATACAAAAAc                                                                                                                                      <  2:87480/151‑1 (MQ=255)                cATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTCCAGACAAATACAAAAAc                                                                                                                                      <  1:8265/151‑1 (MQ=255)                cATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGAGGTAGTTTGTGGCGGCG‑AA‑ATTTTCCAAAAAAATAaaaaaa                                                                                                                                       >  2:767251/1‑150 (MQ=255)                     ccATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTCCAGACAAATACAAAAACT‑GGAg                                                                                                                                <  2:3256/151‑1 (MQ=255)                         tCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTTCAGACAAAAACAAAAAAT‑agaggt                                                                                                                              >  2:676309/1‑147 (MQ=255)                          cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTTCCGACAAATACAAAAAAT‑TGAGTTGcc                                                                                                                           >  2:98305/1‑151 (MQ=255)                              gCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCatga                                                                                                                       <  1:1033218/151‑1 (MQ=255)                              gCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCatg                                                                                                                        <  1:331374/150‑1 (MQ=255)                                  tGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTTCAGAAAAATAAAAAAACT‑GGAg                                                                                                                                >  1:1049915/1‑138 (MQ=255)                                   ggCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCAtga‑tga                                                                                                                   <  2:529706/149‑1 (MQ=255)                                    gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTTTAGACAAAAAAAAAAAAT‑GGAGTTGTCATGA‑TGAGCg                                                                                                                >  1:29366/1‑151 (MQ=255)                                       cAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑GA‑TTTTTTCAGACAAATAAAAAAAAT‑GGAGGTGCCATGA‑TGAGGGaa                                                                                                              >  2:608028/1‑150 (MQ=255)                                       cAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTTCAGACAAAAAAAAAAACT‑GGAGGTGCCCTGA‑TGAGCGaa                                                                                                              >  2:657778/1‑150 (MQ=255)                                       cAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGA‑TGAGCGaaa                                                                                                             <  1:961777/151‑1 (MQ=255)                                        aaTATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑ATTTTTCCCACCAATAAAAAAAAT‑GGGGTTGGCAtga‑tgatgggaa                                                                                                             >  1:747883/1‑144 (MQ=255)                                         atatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTTCAGAAAAATAGAAAAATTGGGGGTTGCCatattagcgga                                                                                                                >  2:620767/1‑139 (MQ=255)                                            ttcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑ATTTTTCAAAAAAAAAAAAAAAAT‑AGAGGTGGCATGG‑TGAggggaaagaa                                                                                                         >  1:621188/1‑141 (MQ=255)                                              cattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGA‑TGAGCGAAAGtaataa                                                                                                      <  2:730914/151‑1 (MQ=255)                                                  cTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTTCAGAAAAAAACAAAAACT‑GGAGTTGGCCTTG‑TGAGGGAAAGTAATAAACa                                                                                                   >  1:552364/1‑150 (MQ=255)                                                    tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGA‑TGAGCGAAAGTAATAAGCAAc                                                                                                 <  2:683586/150‑1 (MQ=255)                                                     aTTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTTCCGACAAATACAAAAACT‑GGAGTTGCCATGA‑TGAGCGAAAGGAATAAGCAACa                                                                                                >  2:119637/1‑150 (MQ=255)                                                          cgcgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTTCAGACAAATACAAAAAAT‑GGAGTTTCCCTGA‑TGAGCGAAAGTAATAAacaaca                                                                                                >  2:746184/1‑145 (MQ=255)                                                          cgcgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTTCAGACAAAAACAAAAAAT‑GGGGGTGCCATGA‑TTGGCGGAAGgaataaaaaaaaggga                                                                                            >  2:663044/1‑139 (MQ=255)                                                          cgcgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGA‑TGAGCGAAAGTAATAAGCAACAGGCAGt                                                                                          <  2:260549/151‑1 (MQ=255)                                                                gATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑ATTTTTCAAACAAAAAAAAAAAAT‑TGAGTTGTCATGG‑TTAGGGGAAGTAATAAAAAAAAGGCAGTGAATa                                                                                     >  1:375047/1‑150 (MQ=255)                                                                   tCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTTCAAACAAAAACAAAAACT‑AGAGTTGTCATGG‑TGAGAGAAAATAATAAAAAACAAGCAGTGaa                                                                                       >  1:853925/1‑145 (MQ=255)                                                                   tCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGG‑AA‑TTTTTTCCCACAAAAAAAAAAAAT‑CGAGATGGCAAGG‑TGAGAGAAAGGAATAAGCAAAAGGGCGGGAATaa                                                                                    >  2:516073/1‑148 (MQ=255)                                                                    cACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGG‑GA‑TTTTTTCACAAAAAAACAAAAAAT‑AGGGTTGTCCTGA‑AGTGCGAAAGAAAAAAGCAACAAGGCGTGTATAAAtt                                                                                 >  2:1089885/1‑150 (MQ=255)                                                                      cGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGA‑TGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGAc                                                                              <  2:888683/151‑1 (MQ=255)                                                                       gAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTTCAGAAAAAAACAAAAAAT‑GGAGGTGCCAAGA‑CGGGGGAAAGTAAAAAGCAACAGGCAATGAATAAGtt                                                                                 >  1:652892/1‑147 (MQ=255)                                                                       gAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGA‑TGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACa                                                                             <  1:807024/151‑1 (MQ=255)                                                                              tAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGA‑TGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGAtt                                                                       <  1:1065683/150‑1 (MQ=255)                                                                              tAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGA‑TGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGAtt                                                                       <  1:372233/150‑1 (MQ=255)                                                                              tAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGA‑TGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGAtt                                                                       <  2:167498/150‑1 (MQ=255)                                                                              tAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGT‑TG‑TTTTTTCAAACAAAAAAAAAAAAT‑AGAGGTGTCAGGA‑TGAACGAAAGAAATAAAGAAAAGGCAGGGGATAAATTGACaggggtt                                                                       >  2:968723/1‑145 (MQ=255)                                                                               aaTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTTCAGACAAATAAAAAAACT‑GGAGTTGCCATGA‑TGAGCGAAAGAAAAAAGCAAAAGGCAGTGAATAAGTTGACAGAGAtt                                                                       >  2:216446/1‑149 (MQ=255)                                                                               aaTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGA‑TGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTg                                                                      <  1:510033/150‑1 (MQ=255)                                                                                  tACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAA‑TTTTTTCA‑ACAAAAACAAAAAAT‑GGGGGTGCCATGA‑TGAGCGAAAGTAAAAAACAACAAGGAGTGGATAAGATGGCaga                                                                           >  1:1112660/1‑142 (MQ=255)                                                                                    cATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGA‑TGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTa                                                                <  1:163431/151‑1 (MQ=255)                                                                                      tatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑ATTTTTCAAACAAAAACAAAAACT‑GGAGGTGGCCTGA‑TGAGGGAAAGAAATAAAAAAAAAGCAGGGAATAAATTGTCAGAGAATTTCGCTa                                                                >  1:703392/1‑149 (MQ=255)                                                                                      tatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGG‑AA‑TTTTTTCCCACAAATACAAAAACT‑GGAGTTGGCCTGG‑TGGGCGAAAGTAATAAGCAAAAAACAGAGGATAAGTTGAGCGAGAATGTCGCCaa                                                               >  1:99262/1‑150 (MQ=255)                                                                                       atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGA‑TGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGCTGACAGAGATTGTCGCTAACt                                                             <  2:460104/151‑1 (MQ=255)                                                                                       atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑ATTTTTCCGACAAATACAAAAAAA‑GGAGGTGCCATGA‑TGAGGGGAAGTAATAAAAAACAGGCAGTGAATAAAATGACAGAGATTGTCGCTa                                                                >  2:699233/1‑148 (MQ=255)                                                                                        ttCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGG‑AA‑ATTTTCCAGACAAAAACAAAAACT‑GGAGGTGCCCTGA‑TGGGGGCAAAAAATAAAAAACAAGCAGAGAATAAATTTACAAAAAATGTTGCTAAAtt                                                            >  1:516942/1‑151 (MQ=255)                                                                                          cACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTTCAAACAAATACAAAAAAT‑GGGGTTTGCATGA‑TTGGCGGAAGGAATAAACAAAAGGGAGTGGATAAGTTGACAGAGATTGTCGCTAACttt                                                           >  1:677927/1‑150 (MQ=255)                                                                                          cACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGA‑TGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACttt                                                           <  1:1023034/150‑1 (MQ=255)                                                                                                ggCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTTCAGACAAAAACAAAAAAT‑GGAGTTTCCATGA‑TTAGCGAAAGGAATAAACAACAAGCAGGGAAAAAAGTTTCAGAGATTGTCGCTAACTTTACcccc                                                     >  2:685794/1‑150 (MQ=255)                                                                                                ggCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTTCAGACAAAAAAAAAAAAT‑GGAGGTGGCATGA‑TGAGCGGAAGTAATAAACAAAAGGGAGGGAATAAATTGACAGAGATTGTTGCTAACTTTACCGcc                                                     >  2:134818/1‑150 (MQ=255)                                                                                                ggCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGA‑TGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGcc                                                     <  1:944039/150‑1 (MQ=255)                                                                                                  aaaaaaaTAAAAAACCACATTTTTAGTGGTAGTTTGGGGCGGTG‑AA‑TTTTTCCAGACAAATAAAAAAACT‑GGAGTTGCCATGA‑TGAGCGAAAGTAATAAGAAAAAGGCAGTAAATAAGTTGACAGAAATTGTCGCTAACTTTACCGCCATg                                                  <  1:890387/150‑1 (MQ=255)                                                                                                   aaaaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGA‑TGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATg                                                  <  2:479177/150‑1 (MQ=255)                                                                                                          ataAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGA‑TGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTAc                                          <  2:426797/151‑1 (MQ=255)                                                                                                           taAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTTCAGACAAATACAAAAAAT‑GGAGGTGGCCTGA‑TGAGGGGAAGTAATAAACAAAAAGCAGTGAAAAAATTGAGAGAGAATGTAGCGAACATTAACGCCACGATTTTTaa                                          >  1:217866/1‑149 (MQ=255)                                                                                                             aaaCCACATTTTTAGTGGTAGTTTGTGGCGGTG‑GA‑ATTTTTCAGACAAAAACAAAAAAT‑GGAGTTGCCATGG‑TGAGGGAAAGGAAAAAACAAACAGCAGAGAAAAAATTGACAAAGAATGTCGCCAACTTTTAAGCCCTGATTTTTAccc                                        >  1:760168/1‑150 (MQ=255)                                                                                                                     tttttAGTGGTAGTTTGTGGCGGGG‑AATTTTTTCCAAAC‑AAGACAAAAAAT‑CGTGTTTTCATGAGAAAGGGAAAGATGTAA‑AAACAACCAGTGAATAAGCTGACAGAGAATGGCGCTAAAATTTTCGCCATGAgttttttcccca                                     >  1:206717/1‑136 (MQ=255)                                                                                                                       tttAGTGGTAGTTTGTGGCGGTG‑GA‑TTTTTTCAGACAAAAAAAAAAACT‑GGGGGTGCCCTGA‑TGAGCGGAAGTAATAAGCAACCGGCAGTGAAAAAGTTGGCAAAGATTGTCGCTAACTTTACCGCCATGATTTTTACCCGAATGCCtgat                             >  1:999698/1‑151 (MQ=255)                                                                                                                         tAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGA‑TGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCtgatg                            <  2:446150/150‑1 (MQ=255)                                                                                                                                     gtgGCGGTG‑AA‑TTTTCCCAGACAAATACAAAACCT‑GGAGTTGCCATGA‑TGAGCGAAATTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTACCTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGAt                 <  1:437199/149‑1 (MQ=255)                                                                                                                                      tgGCGGTG‑AA‑TTTTTTCAGACAAATACAAAAACT‑GGAGTTGCCATGA‑TGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATaa               >  1:760801/1‑150 (MQ=255)                                                                                                                                        gCGGTG‑AA‑TTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGA‑TGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACt            <  2:755530/151‑1 (MQ=255)                                                                                                                                          ggTG‑AA‑TTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGA‑TGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTa           >  2:479534/1‑150 (MQ=255)                                                                                                                                             g‑AA‑TTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGA‑TGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAAc       >  1:164670/1‑151 (MQ=255)                                                                                                                                               aa‑TTTTCCCAAACAAATAAAAAAACT‑GGAGTTGCCAGGA‑TGAGCTAAAGTAATAAACAACAGGCAGTAAATAAGTTAACAGAGATGTTCGCTAATTTAACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaaa        <  1:793163/149‑1 (MQ=255)                                                                                                                                                a‑TTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGA‑TGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAAc       >  2:293488/1‑149 (MQ=255)                                                                                                                                                    tttCCAGACAAATACAAAAACT‑GGAGTTGCCATGA‑TGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCt   >  2:729557/1‑150 (MQ=255)                                                                                                                                                     ttCCAGACAAATACAAAAACT‑GGAGTTGCCATGA‑TGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTa  >  2:845279/1‑150 (MQ=255)                                                                                                                                                     ttCCAGACAAATACAAAAACT‑GGAGTTGCCATGA‑TGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTa  <  2:523535/150‑1 (MQ=255)                                                                                                                                                       |                                                                                                                                                      GTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTCCAGACAAATACAAAAACT‑GGAGTTGCCATGA‑TGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTA  >  NC_000913/3206287‑3206582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑