Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 4,302,658 | 0 | T | G | 43.6% | 87.0 / 13.4 | 56 | T141P (ACC→CCC) | mdtO | membrane translocase (MDR) of MdtNOP efflux pump, PET family |
Reads supporting (aligned to +/- strand): ref base T (7/24); new base G (24/0); total (32/24) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.11e-09 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
AGCGCCTGATGCATTTGCGAAATGGCACGACTGGGAAACCACAGCACGCCGATTAACGTCATCAGCAAGGTTGGATAGAGGCCAACAACGATACACCACAGCGTTAAGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGAAGGTTTGCCCGTAAATAGCGACAATGGCGACGGCGAAAAAGACCAGCCCCAAGCGATGGGTGCGCATCAAAAACATGCAGCCCATCAGGATCGGTCCGGCGATGATCAATCGGATCAACGGTTCGCCGTATGACCATTTGTAGATCAA > NC_000913/4302510‑4302802 | aGCGCCTGATGCATTTGCGAAATGGCACGACTGGGAAACCACAGCACGCCGATTAACGTCATCAGCAAGGTTGGATAGAGGCCAACAACGATACACCACAGCGTTAAGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGAAGGttt > 2:1058308/1‑151 (MQ=255) aGCGCCTGATGCATTTGCGAAATGGCACGACTGGGAAACCACAGCACGCCGATTAACGTCATCAGCAAGGTTGGATAGAGGCCAACAACGATACACCACAGCGTTAAGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGAAGGttt > 2:196494/1‑151 (MQ=255) gcgcCTGATGTATTTGCGAAATGGCACGACTGGGAAGCCACAGCGCGCCGATTAACGTCATCAGCAAGGTTAGGTAGAGGCCGACAACGATACACCACAGCGTTAAGCGCACGACCACTTCCGGGTAGTCGAGCATGGCGGTGAAGGttt < 1:141015/150‑1 (MQ=255) gcgcCTGATGCATTTGCGAAATGGCACGACTGGGAAACCACAGCACGCCGATTAACGTCATCAGCAAGGTTGGATAGAGGCCAACAACGATACACCACAGCGTTAAGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGGAGGgtt > 2:520817/1‑150 (MQ=255) gATGCATTTGCGAAATGGCACGACTGGGAAACCACAGCACGCCGATTAACGTCATCAGCAAGGTTGGATAGAGGCCAACAACGATACACCACAGCGTTAAGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGAAGGGTTTccc > 1:172747/1‑148 (MQ=255) aTTTGCGAAATGGCACGACTGGGAAACCACAGCACGCCGATTAACGTCATCAGCAAGGTTGGATAGAGGCCAACAACGATACACCACAGCGTTAAGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGAAGGTTTGCCCGTaaaaa > 2:454991/1‑148 (MQ=255) tttGCGAAATGGCACGACTGGGAAACCACAGCACGCCGATTAACGTCATCAGCAAGGTTGGATAGAGGCCAACAACGATACACCACAGCGTTAAGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGAAGGGTTGCCCGTAAATAGc > 1:778047/1‑151 (MQ=255) aaaTGGCACGACTGGGAAACCACAGCACGCCGATTAACGTCATCAGCAAGGTTGGATAGAGGCCAACAACGATACACCACAGCGTTAAGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGAAGGTTTGCCCGTAAATAGCGACAAt < 2:6524/151‑1 (MQ=255) aaaTGGCACGACTGGGAAACCACAGCACGCCGATTAACGTCATCAGCAAGGTTGGATAGAGGCCAACAACGATACACCACAGCGTTAAGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGAAGGTTTGCCCGTAAATAGCGACAAt < 2:59551/151‑1 (MQ=255) aaTGGCACGACTGGGAAACCACAGCACGCCGATTAACGTCATCAGCAAGGTTGGATAGAGGCCAACAACGATACACCACAGCGTTAAGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGAAGGTTTGCCCGTAAATAGCGACAATg < 1:316079/151‑1 (MQ=255) ggCACGACTGGGAAACCACAGCACGCCGATTAACGTCATCAGCAAGGTTGGATAGAGGCCAACAACGATACACCACAGCGTTAAGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGAAGGTTTGCCCGTAAATAGCGACAATGGc < 1:322402/150‑1 (MQ=255) ggCACGACTGGGAAACCACAGCACGCCGATTAACGTCATCAGCAAGGTTGGATAGAGGCCAACAACGATACACCACAGCGTTAAGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGAAGGTTTGCCCGTAAATAGCGACAATGGc < 1:4297/150‑1 (MQ=255) ggCACGACTGGGAAACCACAGCACGCCGATTAACGTCATCAGCAAGGTTGGATAGAGGCCAACAACGATACACCACAGCGTTAAGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGAAGGTTTGCCCGTAAATAGCGACAATGGc < 2:970802/150‑1 (MQ=255) cACGACTGGGAAACCACAGCACGCCGATTAACGTCATCAGCAAGGTTGGATAGAGGCCAACAACGATACACCACAGCGTTAAGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGAAGGTTTGCCCGTAAATAGCGACAATGGCGAc < 2:1121338/151‑1 (MQ=255) cGACTGGGAAACCACAGCACGCCGATTAACGTCATCAGCAAGGTTGGATAGAGGCCAACAACGATACACCACAGCGTTAAGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGAAGGGTTGCCCGCAAATAGCGACAAAGgcggcg > 2:924472/1‑150 (MQ=255) tGGGAAACCACAGCACGCCGATTAACGTCATCAGCAAGGTTGGATAGAGGCCAACAACGATACACCACAGCGTTAAGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGAAGGGTTTCCCGCAAAAAGCGACAATGGCGACgg > 2:501731/1‑147 (MQ=255) ggAAACCACAGCACGCCGATTAACGTCATCAGCAAGGTTGGATAGAGGCCAACAACGATACACCACAGCGTTAAGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGAAGGGTTGTCCGCAAAAAACGAAAATGGAGACGGGGaaa > 1:877651/1‑150 (MQ=255) gAAACCACAGCACGCCGATTAACGTCATCAGCAAGGTTGGATAGAGGCCAACAACGATACACCACAGCGTTAAGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGGAGGGTTTCCCGGAAATAACGAAAAAGGCGACGGGGgaaa > 1:777802/1‑150 (MQ=255) aaaCCACAGCACGCCGATTAACGTCATCAGCAAGGTTGGATAGAGGCCAACAACGATACACCACAGCGTTAAGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGGAGGTTTGCCCGGAAATAGAGACAAAGGGGACGGCGaaaaa > 1:1062324/1‑150 (MQ=255) acaGCACGCCGATTAACGTCATCAGCAAGGTTGGATAGAGGCCAACAACGATACACCACAGCGTTAAGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGAAAGGTTGCCCGGAAATAACGACAATGGCGACGGCGgaaaa > 1:914758/1‑145 (MQ=255) cACGCCGATTAACGTCATCAGCAAGGTTGGATAGAGGCCAACAACGATACACCACAGCGTTAAGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGAAGGTTTGCCCGTAAATAGCGACAATGGCGACGGCGAAAAAGACCAGcccc < 2:347379/151‑1 (MQ=255) aCGCCGATTAACGTCATCAGCAAGGTTGGATAGAGGCCAACAACGATACACCACAGCGTTAAGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGGAGGGTTGTCCGTGAATAGGGACAAAGGCGACGGCGAAAAAAACCAGgccc > 2:984237/1‑150 (MQ=255) aCGCCGATTAACGTCATCAGCAAGGTTGGATAGAGGCCAACAACGATACACCACAGCGTTAAGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGGAGGGTTGCCCGTAAATAGCGACAATGGCGGCGGCGAAAAAGAACCGcccc > 2:850499/1‑150 (MQ=255) cGCCGATTAACGTCATCAGCAAGGTTGGATAGAGGCCAACAACGATACACCACAGCGTTAAGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGAAGGTTTGCCCGTAAATAGCGACAATGGCGACGGCGAAAAAGACCAGCCCCaa < 1:532273/151‑1 (MQ=255) gATTAACGTCATCAGCAAGGTTGGATAGAGGCCAACAACGATACACCACAGCGTTAAGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGAAGGGTTGCCCGTAAATAACGACAAATGAGAAGGCGGAAAAAACCAGcccccaacg > 2:1064008/1‑145 (MQ=255) aTTAACGTCATCAGCAAGGTTGGATAGAGGCCAACAACGATACACCACAGCGTTAAGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGAAGGGTTGCCCGTAAATAACGACAAAGGCGACGGCGGAAAAAAACAGCCCCAAGCg > 2:571403/1‑149 (MQ=255) aaCGTCATCAGCAAGGTTGGATAGAGGCCAACAACGATACACCACAGCGTTAAGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGAAGGTTTGCCCGTAAATAGCGACAATGGCGACGGCGAAAAAGACCAGCCCCAAGCGATgg < 1:599199/150‑1 (MQ=255) cGTCATCAGCAAGGTTGGATAGAGGCCAACAACGATACACCACAGCGTTAAGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGAAGGTTTTCCCGCAAATAACGACAAAGGCGACGGCGAAAAAGACCAGACCCCAACGAAggg > 2:250736/1‑149 (MQ=255) catcaGCAAGGTTGGATAGAGGCCAACAACGATACACCACAGCGTTAAGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGAAGGATTGCCCGGAAAAAACGACAATGGCGCCGGAGGAAAAGACACGCCCCACGCGACggggg > 1:79112/1‑146 (MQ=255) cAAGGTTGGATAGAGGCCAACAACGATACACCACAGCGTTAAGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGAAAGGTTGCCCGGAAATAGCGACAATGGCGACGGCGAAAAAAACCAGACCCAAACGATGGGGGCGCATCaa > 1:149572/1‑150 (MQ=255) ggTTGGATAGAGGCCAACAACGATACACCACAGCGTTAAGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGAAAGGTTGCCCGGAAATAACGACAATGGCGACCGCGAAAAAGACCAGCCCCAAGCGATGGGGGCGCCTCaaaaa > 2:538336/1‑150 (MQ=255) tGGATAGAGGCCAACAACGATACACCACAGCGTTAAGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGAAGGGTTGCCCGGAAAAAGACACAAAAGCGACGGCGGAAAAAACCAGCCCCCAACGAGGGGTGGGCATCaaaaaaa > 1:620168/1‑147 (MQ=255) ggATAGAGGCCAACAACGATACACCACAGCGTTAAGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGAAGGGTTGCCCGTAAATAGCGACAAAGGCGGCGGCGGAAAAGACCAGCCCCCAGCGATGGGGGCGCATCAAAAACAAGc > 2:681509/1‑151 (MQ=255) gATAGAGGCCAACAACGATACACCACAGCGTTAAGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGGAGGGTTGCCCCTAAATAGCGACAATGGCGCCGGCGAAAAAAAACCGCCCCCAACGATGGGTGCGCATCaaaaaaa > 1:1082391/1‑145 (MQ=255) agagGCCAACAACGATACACCACAGCGTTAAGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGAAGGTTTGCCCGTAAATAGCGACAATGGCGACGGCGAAAAAGACCAGCCCCAAGCGATGGGTGCGCATCAAAAACATGCAGcc < 2:595463/151‑1 (MQ=255) caacaaCGATACACCACAGCGTTAAGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGGAGGGTTGCCCGCAAAAAGAGAAAATGGCGACGGGGAAAAAGAACAGCCCCAAACGATGGGGGCGCCACAAAAAAAAGCAGccccac > 2:1132062/1‑146 (MQ=255) aacGATACACCACAGCGTTAAGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGAAGGGTTGCCCGGAAAAAGCGACAATGGCGACGGCGGAAAAAACCAGCCCCAAACGATGGGTGCGCATCCAAAAAAATCCGCCCATCAgg > 2:1091943/1‑148 (MQ=255) cacaGCGTTAAGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGAAGGTTTGCCCGTAAAAAGCGACAATGGCGACGGCGAAAAAGAACAGCCCCCAGCGATGGGGGCGCATCAAAAACATGCAGCCCAACAGGATCGGTcc > 2:302574/1‑146 (MQ=255) acaGCGTTAAGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGAAGGTTTGCCCGTAAATAGCGACAATGGCGACGGCGAAAAAGACCAGCCCCAAGCGATGGGTGCGCATCAAAAACATGCAGCCCATCAGGATCGGTCCGGCgat < 1:876426/151‑1 (MQ=255) caGCGTTAAGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGAAGGTTTGCCCGTAAATAGCGACAATGGCGACGGCGAAAAAGACCAGCCCCAAGCGATGGGTGCGCATCAAAAACATGCAGCCCATCAGGATCGGTCCGGCgatg < 1:248485/151‑1 (MQ=255) caGCGTTAAGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGAAGGTTTGCCCGTAAATAGCGACAATGGCGACGGCGAAAAAGACCAGCCCCAAGCGATGGGTGCGCATCAAAAACATGCAGCCCATCAGGATCGGTCCGGCgat < 1:108769/150‑1 (MQ=255) gTTAAGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGAAGGTTTGCCCGTAAATAGCGACAATGGCGACGGCGAAAAAGACCAGCCCCAAGCGATGGGTGCGCATCAAAAACATGCAGCCCATCAGGATCGGTCCGGCGATGATCa < 2:1025555/151‑1 (MQ=255) gTTAAGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGAAGGTTTGCCCGTAAATAGCGACAATGGCGACGGCGAAAAAGACCAGCCCCAAGCGATGGGTGCGCATCAAAAACATGCAGCCCATCAGGATCGGTCCGGCGATGATCa < 2:820820/151‑1 (MQ=255) gTTAAGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGAAGGTTTGCCCGTAAAAAGCGACAATGGCGACGGCGAAAAAGACCAGCCCCCAACCATGGGGGGGCATCAAAAAAAAGCAGCCCCTCAGGATCGGGCCGGCGATGGTc > 2:785527/1‑150 (MQ=255) ttAAGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGAAGGGTTTCCCCTCAAAAAAGACAAAGGCGACGGCGGAAAAGACCAGCCCCAAACGATGGGGGCGCATCAAAAAAATGCAGCCCCTCAGGAACGGGCCGGCGATGATCa > 1:827307/1‑150 (MQ=255) aaGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGGAGGGTTGCCCCTAAATAGCGGCAAAGGCGACCGCGAAAAAGACCAGCCCCCAGCGATGGGGGGGCATCAAAAACATGCAGCCCCTCCGGAACGGGCCGGCGATGATCaa > 1:176791/1‑149 (MQ=255) aaGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGAAGGTTTGCCCGTAAATAGCGACAATGGCGACGGCGAAAAAGACCAGCCCCAAGCGATGGGTGCGCATCAAAAACATGCAGCCCATCAGGATCGGTCCGGCGATGATCAATc < 2:79117/151‑1 (MQ=255) aaGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGAAGGTTTGCCCGTAAATAGCGACAATGGCGACGGCGAAAAAGACCAGCCCCAAGCGATGGGTGCGCATCAAAAACATGCAGCCCATCAGGATCGGTCCGGCGATGATCAATc < 1:1027307/151‑1 (MQ=255) aGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGAAGGGTTGCCCCTAAATAGCGACAATGGCGACGGCGAAAAAAACCCGCCCCCAGCGATGGGGGCGCATCAAAAAAATGCAGCCCCTCAGGATCGGTCCGGCGATGATCAATCg > 2:668019/1‑151 (MQ=255) cgcACGACCACTTCCGGATAGTCGAGCATGGCGGGGAAGGTTTGCCCGTAAATAGCGACAATGGCGACGGCGAAAAAGACCAGCCCCAAGCGATGGGTGCGCATCAAAAACATGCAGCCCATCAGGATCGGTCCGGCGATGATCAATCGGa < 1:115297/151‑1 (MQ=255) gACCACTTCCGGATAGTCGAGCATGGCGGGGGAGGGTTTCCCGGAAAAAACGACAAAGGGGACGGCGAAAAAAACCAACCCCCAACGATGGGGGCGCATCAAAAAAATGCAGCCCATCAGGATCGGGCCGGCGGAGATCAAACGGGTCaa > 2:1060371/1‑150 (MQ=255) gACCACTTCCGGATAGTCGAGCATGGCGGGGAAGGTTTGCCCGTAAATAGCGACAATGGCGACGGCGAAAAAGACCAGCCCCAAGCGATGGGTGCGCATCAAAAACATGCAGCCCATCAGGATCGGTCCGGCGATGATCAATCGGATCAAc < 1:135038/151‑1 (MQ=255) cACTTCCGGATAGTCGAGCATGGCGGGGGAGGGTTGCCCGTAAAAAGCGACAATGGCGGCTGCGGAAAAAACCAGCCCCCAGCGATGGGTGCGCATCAAAAAAATGCAACCCATCAAGAACGGTCCCGGGATGATTAATCGGATCAAccg > 2:814030/1‑148 (MQ=255) aCTTCCGGATAGTCGAGCATGGCGGAGAAGGGTTGCCCCGAAATAGCGACAATGGGGCAGGCGAAAAAGACCAAGCCCAAACGACGGGGGGGCCTCCAAAAAAAACAGCCCATCCGGGACGGTCCGGCGGAGAACCAACGGGTCAACCGtt > 1:501318/1‑151 (MQ=255) tCCGGATAGTCGAGCATGGCGGGGAAGGTTTGCCCGTAAATAGCGACAATGGCGACGGCGAAAAAGACCAGCCCCAAGCGATGGGTGCGCATCAAAAACATGCAGCCCATCAGGATCGGTCCGGCGATGATCAATCGGATCAACGGTTc < 2:49559/149‑1 (MQ=255) ccGGATAGTCGAGCATGGCGGGGAAGGTTTGCCCGTAAATAGCGACAATGGGGACGGCGGAAAAAACCAGCCCCAAGCGATGGGGGCGCATCAAAAAAATACAGCCCAACAGGATCGGGACCGCGAAGATCAATAGGATCAACAGGTCGcc > 2:530143/1‑151 (MQ=255) ggATAGTCGAGCATGGCGGGGAAGGTTTGCCCGTAAATAGCGACAATGGCGACGGCGAAAAAGACCAGCCCCAAGCGATGGGTGCGCATCAAAAACATGCAGCCCATCAGGATCGGTCCGGCGATGATCAATCGGATCAACGGTTCGCCGt < 2:603706/151‑1 (MQ=255) aTAGTCGAGCATGGCGGGGAAGGTTTGCCCGTAAATAGCGACAATGGCGACGGCGAAAAAGACCAGCCCCAAGCGATGGGTGCGCATCAAAAACATGCAGCCCATCAGGATCGGTCCGGCGATGATCAATCGGATCAACGGTTCGCCGTAt < 1:73887/151‑1 (MQ=255) ggAAGGTTTGCCCGTAAATAGCGACAATGGCGACGGCGAAAAAGACCAGCCCCAAGCGATGGGTGCGCATCAAAAACATGCAGCCCATCAGGATCGGTCCGGCGATGATCAATCGGATCAACGGTTCGCCGTATGACCATTTGTAGATCaa < 1:770169/151‑1 (MQ=255) gAAGGTTTGCCCGTAAATAGCGACAATGGCGACGGCGAAAAAGACCAGCCCCAAGCGATGGGTGCGCATCAAAAACATGCAGCCCATCAGGATCGGTCCGGCGATGATCAATCGGATCAACGGTTCGCCGTATGACCATTTGTAGATCaa > 2:945598/1‑150 (MQ=255) | AGCGCCTGATGCATTTGCGAAATGGCACGACTGGGAAACCACAGCACGCCGATTAACGTCATCAGCAAGGTTGGATAGAGGCCAACAACGATACACCACAGCGTTAAGCGCACGACCACTTCCGGATAGTCGAGCATGGCGGGGAAGGTTTGCCCGTAAATAGCGACAATGGCGACGGCGAAAAAGACCAGCCCCAAGCGATGGGTGCGCATCAAAAACATGCAGCCCATCAGGATCGGTCCGGCGATGATCAATCGGATCAACGGTTCGCCGTATGACCATTTGTAGATCAA > NC_000913/4302510‑4302802 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |