Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	345,328	0	C	A	40.5%	124.7 / 43.3	84	intergenic (+337/‑76)	yahL/yahM	uncharacterized protein/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base C (40/10);  new base A (0/34);  total (40/44)
Fisher's exact test for biased strand distribution p-value = 8.96e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAAAAATGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGG‑TCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCT‑GTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGTGGAACATT  >  NC_000913/345179‑345474                                                                                                                                                        |                                                                                                                                                   aaaaaaTGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGG‑TCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCT‑GTAAGGCTCATATc                                                                                                                                                    <  1:668789/150‑1 (MQ=255) aaaaaaTGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGG‑TCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCT‑GTAAGGCTCATATc                                                                                                                                                    >  2:342840/1‑150 (MQ=255)  aaaaaTGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGG‑TCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCT‑GTAAGGCTCATATCa                                                                                                                                                   >  2:721712/1‑150 (MQ=255)   aaaaTGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAACTCCATCATGTTTGTGTGGGG‑TCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCT‑GTAAGGCTCATATCaaa                                                                                                                                                 <  1:68424/151‑1 (MQ=255)     aaTGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGG‑TCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCT‑GTAAGGCTCATATCaaaaa                                                                                                                                               <  1:824647/151‑1 (MQ=255)     aaTGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGG‑TCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCT‑GTAAGGCTCATATCaaaa                                                                                                                                                >  1:276125/1‑150 (MQ=255)          tctcATGCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGG‑TCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCT‑GTAAGGCTCATATCAAAAACCGcc                                                                                                                                          >  1:842403/1‑151 (MQ=255)           ctcATGCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCAGCATGTTTGTGTGGGG‑TCTTTTCTGTATCTTACACATCGCACTCAAGCCTGACAGAAAATATGCT‑GTAAGGCTCATATCAAAAACCGcc                                                                                                                                          >  1:493673/1‑150 (MQ=255)                    aTGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGG‑TCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCT‑GTAAGGCTCATATCAAAAACCGCCATTAGCtca                                                                                                                                 >  1:105928/1‑150 (MQ=255)                           gtgAATGTGTTGGTGGACATTCATATGAAAAAAATAATAATTCCACCTTGTTTGTGTGGGG‑TTTTTTCTGTTTCTTCCGCACCGCCCTAAAGCCTGAAAGAAAATATGCT‑GAAAGGCTAATATCAAAAACCGCCATTAGCTCATCAGGa                                                                                                                           <  2:69805/149‑1 (MQ=255)                              aaTGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGG‑TCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCT‑GTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGc                                                                                                                      >  2:763123/1‑151 (MQ=255)                              aaTGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGG‑TCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCT‑GTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGc                                                                                                                      >  1:252794/1‑151 (MQ=255)                              aaTGTGTTGGTTGACATTAATATGAAAAAAATCATAATTCCATCATGTTTGTGGGGGT‑TTTTTTCTGTATCTTACGCATCGCACTCAAGCCTGAAAAAAAATATGTT‑GTAAGGCTAATATAAAAAACCGCCATTAGCTCATCAGGAaga                                                                                                                        <  2:126361/149‑1 (MQ=255)                                  tttggtacgaattcaatatGAAAAAAATATTCACCCCACCATTTTGGGGTGGTT‑TTTTTTTTTGATATTTCGAATCCTACCAAAGCGTGAAAAAAAATATGCT‑GTAAGCCTAAGTTAAAAACCCGCCATTAGCTCATCAGGAAGAGCAga                                                                                                                   <  2:354934/135‑1 (MQ=255)                                  tgtTGGTTGACATTCATATAAAAAAAATCATAATCCCATCATGTTTGTGGGGGG‑TCTTTTCTGTATCTTCCGCATCGCACTCAAGCCTGAAAAAAAATATGCT‑GTAAGGCTCATATAAAAAACCGCCATTAGCTCATCAGGAAGAGc                                                                                                                      <  2:594105/147‑1 (MQ=255)                                    ttggttgAATTTCATATGAAAAAAATCAAAATTCCATCATGTTTGGGGGGGT‑TTTTTTCTGTATCTTACCCACCGCCCACAAGCCTGAAAAAAAATAGGCT‑GTAAGGCACAAATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAgac                                                                                                                  <  1:692570/149‑1 (MQ=255)                                      ggttgACATTAATATAAAAAAAATCATAATCCCATCATGTTTGTGTGGGT‑TCTTTTCTTTATCTTCCGCACCGCACTCAAGCCGGACAAAAAATATGCT‑GAAAGGCTCATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAgacgac                                                                                                               <  1:259314/150‑1 (MQ=255)                                       gttgACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGG‑TCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCT‑GTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACa                                                                                                              >  1:746809/1‑150 (MQ=255)                                           aCATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGG‑TCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCT‑GTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAAcc                                                                                                           >  1:731924/1‑149 (MQ=255)                                              ttCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGG‑TCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCT‑GTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGAt                                                                                                      >  2:530863/1‑151 (MQ=255)                                              ttCATATAAAAAAAACAATAATTCTATCA‑GTTTTTGGGGGGTTCTTTTCTCTATTTTACCCATCCAACTCAAGCCGGAAAAAAAATATGAT‑GTATGTCTAATAACAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGa                                                                                                       <  2:823054/150‑1 (MQ=255)                                                         aaaaTCATAATTCCATCATGTTTGTGTGGGG‑TCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCT‑GTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGt                                                                                           >  2:199112/1‑151 (MQ=255)                                                           aacaaTAATCCCATCATTTTTGGGTGGGG‑TTTTTTCTTTACTTTACTCATCTCTCTAAAGCCTGAAAAAAAATATGCT‑GTGGGGATCATAAAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTa                                                                                          <  2:369355/146‑1 (MQ=255)                                                              cataatcccttattttttGGGTGGGG‑TTTTTTTTGTATCTTCCGCACCCCACCAAACCCAAAAAAAAAATATGCT‑AAAAGCATAATAAAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACgg                                                                                       <  1:591256/135‑1 (MQ=255)                                                               aTAATTCCATCATGTTTGTGTGGGT‑TCTTTTCTGTATCTTACGCACCCCACCCAACCCGAACAAAAAATATGCT‑GTAAGGCTAATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACgg                                                                                       <  1:1074306/149‑1 (MQ=255)                                                                tAATTCCATCATGTTTGTGTGGGG‑TCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCT‑GTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGt                                                                                    >  1:61972/1‑151 (MQ=255)                                                                 aaTTCCATCATGTTTGTGTGGGG‑TCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCT‑GTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGtt                                                                                   >  2:15094/1‑151 (MQ=255)                                                                 aaTCCCATCATTTTTGTGTGGGT‑TTTTTTCTTTATCTTCCGCACCCCACTCAACCCTGACAAAAAATTTGTT‑GAAAGCCTCATAAAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGtt                                                                                   <  2:803442/151‑1 (MQ=255)                                                                  aTTCCATCATGTTTGTGTGGGG‑TCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCT‑GTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTc                                                                                  >  2:216857/1‑151 (MQ=255)                                                                  aTTCCATCATGTTTGTGTGGGG‑TCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCT‑GTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTc                                                                                  >  1:19357/1‑151 (MQ=255)                                                                     ccATCTTGTTTGTGGGGGG‑TTTTTTCTGTATCTTACGCACCGCCCTAAAGCCTGAAAAAAAATATGCT‑GTAAGGCTAATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCg                                                                                 <  2:393623/149‑1 (MQ=255)                                                                     ccATCATTTTTGTGGGGGG‑TTTTTTCTGTATCTTCCGCACCGCACTAAACCCTGACAAAAAATAGGCT‑TTAAGCCTCATATAAAAACCCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAg                                                                               <  2:30597/151‑1 (MQ=255)                                                                     ccATCATGTTTGTGTGGGG‑TCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCT‑GTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGtt                                                                                   >  1:915337/1‑147 (MQ=255)                                                                     ccATAATTTTTGTGTGGGG‑TTTTTTTTTTATCTTCCGCACCACACTCAACCCAGACAAAAAATATGCT‑GTAGGGCTCATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCg                                                                                 <  2:868769/149‑1 (MQ=255)                                                                       atcatGTTTGTGTGGGG‑TCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCT‑GTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGc                                                                             >  1:337277/1‑151 (MQ=255)                                                                         catGTTTGTGTGGGG‑TCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCT‑GTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGcc                                                                            >  1:345683/1‑150 (MQ=255)                                                                         catGTTTGTGTGGGG‑TCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCT‑GTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCt                                                                           >  1:280623/1‑151 (MQ=255)                                                                         catGTTTGTGTGGGG‑TCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCT‑GTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCt                                                                           >  2:555051/1‑151 (MQ=255)                                                                             tttGTGGGGGT‑TTTTTTCTTTATCTTCCGCATCGCACACAAGCCTGACAAAAAATATGCT‑GAAAGGCTCATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTc                                                                          <  2:389055/148‑1 (MQ=255)                                                                             tttGGGTGGGG‑TTTTTTCTGTATCTTACGCATCGCACTAAACCCTGACAAAAAATATGCT‑GTAAGGCTCATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCt                                                                           <  2:418422/147‑1 (MQ=255)                                                                              ttGTGTGGGG‑TCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCT‑GTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTc                                                                          >  1:388983/1‑147 (MQ=255)                                                                               tgtgtgGGT‑TTTTTTCTGTATCTTCCGCATCGCACTCAAGCCTGACAAAAAATATGCT‑GTAAGGCTAATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATg                                                                      <  1:509646/150‑1 (MQ=255)                                                                               tgtgtgGGG‑TCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCT‑GTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATgg                                                                     >  1:853580/1‑151 (MQ=255)                                                                                gtgtgGGG‑TCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCT‑GTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGc                                                                    >  2:687309/1‑151 (MQ=255)                                                                                 tgtgGGT‑TTTTTTCTGTATCTTACGCATCGCCCTCAAGCCTGAAAGAAAATATGCT‑GTAGGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATg                                                                      <  2:940068/148‑1 (MQ=255)                                                                                 tgtgGGG‑TCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCT‑GTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCg                                                                   >  1:463643/1‑151 (MQ=255)                                                                                  gggggg‑TTTTTTCTGTATCTTACGCATCGCACTAAAGCCGAAAAAAAAATATGTT‑GTAAGGCTAATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGc                                                                    <  1:1109761/147‑1 (MQ=255)                                                                                    gggg‑TCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCT‑GTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTc                                                                >  1:881232/1‑151 (MQ=255)                                                                                      gg‑TCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCT‑GTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTcc                                                               >  2:848091/1‑150 (MQ=255)                                                                                            tttCTGTATCTTACGCATCCCACTCAAGCCTGAAAAAAAATATGCT‑GTAAGGCTCATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAAc                                                            <  1:133407/148‑1 (MQ=255)                                                                                             ttCTGTTTCTACCGAATCGCACACAAGCCGAAAAAAAAATATGCT‑GAAAGGCTAATAAAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACttt                                                         <  1:20660/150‑1 (MQ=255)                                                                                             ttCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCT‑GTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACtttt                                                        >  1:1100453/1‑151 (MQ=255)                                                                                              tCTGTATCTTACGCATCCCACTAAAGCCTAAAAAAAAATATGTT‑GTAAGGCTCATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACtttt                                                        <  2:916942/150‑1 (MQ=255)                                                                                                tGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCT‑GTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCa                                                      >  2:548796/1‑150 (MQ=255)                                                                                                  tATCTTACGCATCGCACTCAAGCCTGACAAAAAATATGCT‑GTAAGGCTCATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCaaa                                                    <  2:458022/150‑1 (MQ=255)                                                                                                    tCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCT‑GTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGCGGTTCGAGGCCTCGATGGCGGGCCAACTTTTCAAAAc                                                  >  2:929814/1‑150 (MQ=255)                                                                                                     cTTACGCATCGCACTCAAGCCTGACAGAAAATATGCT‑GTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAActc                                                >  2:919253/1‑151 (MQ=255)                                                                                                        aCGCATCGCACTCAAGCCTGACAGAAAATATGCT‑GTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACtctt                                              >  2:359240/1‑150 (MQ=255)                                                                                                         cgcaccgcacTAAACCCAGACAGAAAATA‑GCTGGTAAGGCTCATAAAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACtctt                                              <  1:418078/149‑1 (MQ=255)                                                                                                         cGCATCCCACACAACCCTGACAAAAAATATGCT‑GAAAGGCTAATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACtcttc                                             <  1:833865/150‑1 (MQ=255)                                                                                                          gCATCGCACTCAAGCCTGACAGAAAATATGCT‑GTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTg                                           >  2:849824/1‑151 (MQ=255)                                                                                                           caccccACCCAAGCCTGAAAGAAAATATGCT‑GAAAGGCTAAAATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAAct                                                 <  1:517209/139‑1 (MQ=255)                                                                                                            accccaccaaacccTAACAAAAAATATGTT‑GAAGGTCTCATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGaa                                         <  1:111942/139‑1 (MQ=255)                                                                                                              cGCACGAAAGCCTGAAAAAAAATAGGCT‑TTAAGACTCATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGaa                                         <  2:345286/149‑1 (MQ=255)                                                                                                                cACTCAAGCCTGACAGAAAATATGCT‑GTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATc                                       >  1:318471/1‑149 (MQ=255)                                                                                                                     aaGCCTGACAGAAAATATGCT‑GTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATccc                                >  2:69540/1‑151 (MQ=255)                                                                                                                      aGCCTGACAGAAAAAATGCG‑GACAGGCCCAAATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTATGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATccc                                <  1:487225/150‑1 (MQ=255)                                                                                                                        ccTGACAGAAAATATGCT‑GTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCtt                             >  1:870375/1‑151 (MQ=255)                                                                                                                             cAGAAAATATGCT‑GTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCAAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTtattat                        >  1:1034128/1‑151 (MQ=255)                                                                                                                              aaaaaaatttacgtaaaGGCTCATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTtattat                        <  2:671339/136‑1 (MQ=255)                                                                                                                              aaaaaaTATGCT‑GTAGGTCTCATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTatc                       <  1:984976/149‑1 (MQ=255)                                                                                                                                aaaaTATGCT‑GTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTatcat                     >  2:510122/1‑151 (MQ=255)                                                                                                                                aaaaTATGCT‑GAAAGGCTAATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTatcat                     <  2:842516/151‑1 (MQ=255)                                                                                                                                 aaaTATGTT‑GAAAGGCTCATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTatcatc                    <  2:958251/151‑1 (MQ=255)                                                                                                                                  aaTATGCT‑GTAAGGCTCATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATcc                   <  1:872800/151‑1 (MQ=255)                                                                                                                                  aaTATGCT‑GAAAGGCTCATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATcc                   <  2:671085/151‑1 (MQ=255)                                                                                                                                     atGCT‑GTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTg                >  1:198571/1‑151 (MQ=255)                                                                                                                                          tgaagggCTAATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCa              <  1:424005/144‑1 (MQ=255)                                                                                                                                           gaaagcttaaaatCAAAAGCCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTg                <  1:224102/135‑1 (MQ=255)                                                                                                                                            tAAGGCTAATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCa              <  1:1079989/147‑1 (MQ=255)                                                                                                                                             aaGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAg           <  2:415455/149‑1 (MQ=255)                                                                                                                                             aaGGCTAATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAg           <  1:143281/149‑1 (MQ=255)                                                                                                                                              aGGCTAATATAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCa              <  1:889112/145‑1 (MQ=255)                                                                                                                                                   cATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGTGGAACAt   >  1:876546/1‑151 (MQ=255)                                                                                                                                                    atatCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGTGGAACAtt  >  1:1005235/1‑151 (MQ=255)                                                                                                                                                    atatAAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGTGGaa      <  1:841703/147‑1 (MQ=255)                                                                                                                                                        |                                                                                                                                                   AAAAAATGATCTCATGCAGATGTTTTGTGAATGTGTTGGTTGACATTCATATGAAAAAAATCATAATTCCATCATGTTTGTGTGGGG‑TCTTTTCTGTATCTTACGCATCGCACTCAAGCCTGACAGAAAATATGCT‑GTAAGGCTCATATCAAAAACCGCCATTAGCTCATCAGGAAGAGCAGACGACAACCAGGATTGTTGTATGGTACGGGGTTCGAGGCCTCGATGGCGGTCCAACTTTTCAAAACTCTTCTGAATCAGATCCCCTTATTATCATCCCTGCAAAGTGGAACATT  >  NC_000913/345179‑345474

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑