Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	354,288	0	T	G	44.1%	91.5 / 19.2	59	V528G (GTG→GGG)	prpE	propionate‑‑CoA ligase
Reads supporting (aligned to +/- strand):  ref base T (4/29);  new base G (26/0);  total (30/29)
Fisher's exact test for biased strand distribution p-value = 7.84e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCC  >  NC_000913/354138‑354428                                                                                                                                                       |                                                                                                                                              aCTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGt                                                                                                                                               <  2:63710/151‑1 (MQ=255)      ggCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAg                                                                                                                                          <  2:558343/151‑1 (MQ=255)       gCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGa                                                                                                                                         <  2:869895/151‑1 (MQ=255)         aTCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATg                                                                                                                                       >  2:997224/1‑151 (MQ=255)           ccGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATgc                                                                                                                                      <  1:1101841/150‑1 (MQ=255)             gTGACGCTGACGGTTATCACTTTATTCTCGGGCGCGCTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCt                                                                                                                                   <  2:965762/151‑1 (MQ=255)             gTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGGAAGAt                                                                                                                                        >  2:962599/1‑146 (MQ=255)              tGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGCGGCGGTGGGTGGGGGGGAAGAttgg                                                                                                                                     >  1:269125/1‑145 (MQ=255)                  gCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAg                                                                                                                              <  2:289838/151‑1 (MQ=255)                  gCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGGAAGATGGGCTgga                                                                                                                                >  2:1118248/1‑147 (MQ=255)                  gCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGAAGGGCTGaa                                                                                                                                >  1:732725/1‑149 (MQ=255)                        ggTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGTTGGGGGGGAAGATGCGCTGAAAGGGCAgg                                                                                                                        >  2:413737/1‑151 (MQ=255)                         gTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCCAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAAATGCGCTGGAAAGGCAgg                                                                                                                        >  2:959482/1‑150 (MQ=255)                           tATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAggtg                                                                                                                      <  2:723958/150‑1 (MQ=255)                           tATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGAAGCGGTGAAAggggcggggg                                                                                                                     >  1:895829/1‑144 (MQ=255)                             tCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGGGCTGGAAAGGGAggggggg                                                                                                                   >  2:409050/1‑149 (MQ=255)                                   tATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGAAAGATGCGCTGAAAGGGCAGggggcgggggc‑g                                                                                                            >  2:350112/1‑151 (MQ=255)                                      tctcGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGGAAGATGAGGCGAAAGGGGAggtggcggagggggtt                                                                                                          >  2:281528/1‑146 (MQ=255)                                        tcGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGAAGTGCTGGAAGGGGAGGGGGGGGGGGG‑Gttt                                                                                                         >  1:1064127/1‑149 (MQ=255)                                         cGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑Gttt                                                                                                         <  1:73144/148‑1 (MQ=255)                                          gggCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGt                                                                                                       <  1:930674/149‑1 (MQ=255)                                            gcgcACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCAtt                                                                                                   <  1:642352/151‑1 (MQ=255)                                             cgcACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGGAAGATGCGCTGAAAGGGCAAGTGGGGGGGGG‑GGTTGTCAtt                                                                                                   >  2:790436/1‑150 (MQ=255)                                                 cTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGGGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGGGGCGGGGGGTGGGGTGAAAGAGGGGCCGGAAAGGCGGGGGGGGGGGGC‑GTTTGTCAtt                                                                                                   >  2:508931/1‑146 (MQ=255)                                                  tGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGATGCGCTGGAAGGGGAGGGGGCGGGGGG‑GTTTGTTATTcc                                                                                                 >  1:174697/1‑147 (MQ=255)                                                   gACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAag                                                                                            <  1:631560/151‑1 (MQ=255)                                                         gTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGc                                                                                       <  2:539300/150‑1 (MQ=255)                                                           aaTTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGAGGGTTGGGGGAAAGAAGCGCTGAAAGGGCCGGGGGCGGGGGC‑GGTTGTCATTTCGAAAGAGAGCg                                                                                      >  2:129032/1‑149 (MQ=255)                                                            aTTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACa                                                                                   <  2:381933/151‑1 (MQ=255)                                                               aaCGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTc                                                                                <  1:585893/151‑1 (MQ=255)                                                                 cGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTg                                                                              <  2:890787/151‑1 (MQ=255)                                                                    tGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTGGGGGGGAAGAAGCGCTGCAAGGGGAGGGGGGGGGGGCGGTTGGTCA‑TCCGGAAAAGAGGGACCGTCTg                                                                              >  1:785577/1‑148 (MQ=255)                                                                       cGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGa                                                                         <  2:569830/150‑1 (MQ=255)                                                                        gggCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATc                                                                       <  2:680377/151‑1 (MQ=255)                                                                         ggCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCg                                                                      <  1:773975/151‑1 (MQ=255)                                                                               ggCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTTGGGTGGAAGATGCGCTGAAAGGGCAGGGGGCGGTGGG‑GTGTGTCATTCCGAAAGAGAGCGACACTCTGGAAGATCg                                                                      >  2:247638/1‑145 (MQ=255)                                                                               ggCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGGAAGATGCGCTGAAAGGGGAGGGGGGGGTGGG‑GGTTGTTATTCCGAAAGAGAGCGAGAGTCTGGAAGATCGCGAtg                                                                 >  1:364042/1‑150 (MQ=255)                                                                                   ggggACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTgg                                                              <  2:914445/149‑1 (MQ=255)                                                                                       aCGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGAGCTGAAAGGGCAGGGGGGGGTGGG‑GGTTGGCATTCCGGAAGAGGGGGGCAAACCTGAAGAGCGTGAt                                                                  >  2:82460/1‑141 (MQ=255)                                                                                           gcgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCa                                                    <  1:1129253/151‑1 (MQ=255)                                                                                            cgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAGATGCGCTGAAAGGGCAGGGGGCGGTGGG‑GTTTGTCATTCCGAAAGAGAGCGGCAGTCTGGAAGATCGTGAGGTGGGGCACTc                                                       >  1:1110072/1‑147 (MQ=255)                                                                                            cgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGGTGGGGGGAAAGATGCGCTGAAAGGGCAGGTGGCGGGGGC‑GTTTGTTATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCa                                                    >  2:676280/1‑150 (MQ=255)                                                                                            cgAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGCGGTTGGGGGGGAAGATGCGCTGGAAGGGCCGGGGGCGGGGGC‑GTTTGTCCTTTCGGAAGAGAGCGGCAATCTGGGAAATCGTGGTGTGGGGCACTCGc                                                     >  2:741976/1‑149 (MQ=255)                                                                                              agaTTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGGTTGGGTGAAAAAAGCGCTGAAAAGGCCGGGGGGGGGGGG‑GTTTGTCATTTCGAAAAAGAGCGACAGTCTGGGAGATCGGGATGTgg                                                              >  1:996160/1‑138 (MQ=255)                                                                                                  tGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGGGGTTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGCGGGGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGACTGGGAGAACGTGATGTGGCGCACTCGCAagag                                                >  2:894607/1‑148 (MQ=255)                                                                                                   gAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAg                                             <  2:280910/150‑1 (MQ=255)                                                                                                    aaGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGc                                           <  1:892567/151‑1 (MQ=255)                                                                                                            aTCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATg                                    <  2:1043616/150‑1 (MQ=255)                                                                                                                ccAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGgc                                  <  1:150533/148‑1 (MQ=255)                                                                                                                ccAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCt                               <  1:849219/151‑1 (MQ=255)                                                                                                                 cAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGCGGGGGC‑GGTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGAACGTGATGTGGCGCACTCGCAAGAGAAAGCGATTATGgcgc                                >  2:366305/1‑149 (MQ=255)                                                                                                                      aTCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGCTGAAAGGGGAGGGGGGGGGGGG‑GGTTGTCTTTCCGAAAGAGAGCGACAGGCTGGGAGATCGTGATGTGGGGCACTCGCAAGAGAAGGGGGTTATGGGGCtggggg                          >  1:938423/1‑150 (MQ=255)                                                                                                                         cGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGAc                        <  2:857935/149‑1 (MQ=255)                                                                                                                          gggCGTTGCCGAAGTGGCGGTGGGTGGGGGGGAAAATGCGCTGAAAGGGCAGGGGGGGGGGGC‑GTTTGTCATTCCGGAAGAGAGGGACAGTCTGGAAGATCGTGGTGTGGGGCACTCGCAAGAGAAGGCGGTTATGGGGCTGGGGGACAg                      >  1:528995/1‑150 (MQ=255)                                                                                                                           ggCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGcc                    <  2:1080521/151‑1 (MQ=255)                                                                                                                            gCGTTGCCGAAGTGGCGGTGGTTGGGGGGAAAGATGCGCTGAAAGGGCAGGGGGCGGTGGG‑GTTTGTCATTTCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGGGGACAGcc                    >  2:716737/1‑150 (MQ=255)                                                                                                                                tGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGAt                <  1:1049051/150‑1 (MQ=255)                                                                                                                                           cGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTgg    >  1:466521/1‑151 (MQ=255)                                                                                                                                           cGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTgg    <  2:1117563/151‑1 (MQ=255)                                                                                                                                             gtggtTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGgcc  <  1:86835/151‑1 (MQ=255)                                                                                                                                                       |                                                                                                                                              ACTGGGGCATCCGTGACGCTGACGGTTATCACTTTATTCTCGGGCGCACTGACGATGTAATTAACGTTGCCGGGCATCGGCTGGGGACGCGCGAGATTGAAGAGAGTATCTCCAGCCATCCGGGCGTTGCCGAAGTGGCGGTGGTTGGGGTGAAAGATGCGCTGAAAGGGCAGGTGGCGGTGGC‑GTTTGTCATTCCGAAAGAGAGCGACAGTCTGGAAGATCGTGATGTGGCGCACTCGCAAGAGAAGGCGATTATGGCGCTGGTGGACAGCCAGATTGGCAACTTTGGCC  >  NC_000913/354138‑354428

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑