Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,097,581	0	A	G	38.9%	130.8 / 15.3	72	noncoding (72/88 nt)	serX	tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base A (4/40);  new base G (28/0);  total (32/40)
Fisher's exact test for biased strand distribution p-value = 4.76e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATA‑CACA‑C‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCA‑CCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAA  >  NC_000913/1097436‑1097730                                                                                                                                                   |                                                                                                                                                          cACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTc                                                                                                                                                        <  2:117618/149‑1 (MQ=11) cACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTc                                                                                                                                                        <  2:774020/149‑1 (MQ=11)   cTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGaa                                                                                                                                                     <  1:493134/150‑1 (MQ=32)     gCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAAcc                                                                                                                                                   <  2:103505/150‑1 (MQ=33)        ttCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGT‑GGGGGGGGTTCCGAccccc                                                                                                                                                >  1:876497/1‑150 (MQ=9)          cACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGAt                                                                                                                                             <  2:808962/151‑1 (MQ=38)             ggTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATAc                                                                                                                                           <  2:562070/150‑1 (MQ=40)              gTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGt                                                                                                                                         <  2:985482/151‑1 (MQ=40)                     cAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGTTCGAACCCCCCATAAGTTGcc                                                                                                                                     >  2:1056501/1‑148 (MQ=255)                     cAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGta                                                                                                                                  <  1:490656/151‑1 (MQ=255)                      aaCTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGtat                                                                                                                                 <  2:957786/151‑1 (MQ=255)                        cTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGATCGAAACCCCCGGACATTGCCGtat                                                                                                                                 >  2:560896/1‑149 (MQ=11)                         tGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGTTCGAACCCCCCACACGTTTCCGtata                                                                                                                                >  1:980997/1‑149 (MQ=255)                         tGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGGTTGAAACCCCCATACATTGCCGtgtaa                                                                                                                               >  2:695558/1‑149 (MQ=11)                            cTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGTTCGGACCCCCCATaagcttcc                                                                                                                                     >  2:531953/1‑134 (MQ=11)                             tGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGGTCGAACCCCCCCGACGTTTCCCTATa‑cac                                                                                                                            >  1:870185/1‑148 (MQ=11)                              gTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATA‑CACA‑C‑t                                                                                                                       <  1:868786/150‑1 (MQ=255)                              gTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATA‑CACA‑C‑t                                                                                                                       <  1:646609/150‑1 (MQ=255)                                 gCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATA‑CACA‑C‑ttt                                                                                                                     <  2:601921/149‑1 (MQ=255)                                   cAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGG‑GGGGGGGAGTCGAACCCCCCATAAGTTGCCGCATA‑CACA‑C‑ctt                                                                                                                     >  2:599885/1‑147 (MQ=14)                                      ttGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCACCGATACGTTGCCGTATA‑CACA‑C‑TTTCCAgg                                                                                                                <  1:46458/149‑1 (MQ=255)                                       tGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGGTTGAACCCCCCATACGTTGCCGTATT‑AACA‑C‑TTTCCCGGCg                                                                                                              >  1:723022/1‑150 (MQ=14)                                         tCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGTTTGAACCCCCCCTACCTTTCCCTTTA‑CAAACC‑TTTcccgcgt                                                                                                              >  1:441420/1‑144 (MQ=11)                                           gAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGG‑GGGGGGGGTTTGGACCCCCCCTACGTTTCCCTATA‑AACA‑C‑TTTTCCGGCGt                                                                                                             >  2:27407/1‑147 (MQ=14)                                                ccGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATA‑CACA‑C‑TTTCCAGGCGTGCTCCTTCa                                                                                                    <  2:368445/151‑1 (MQ=255)                                                 cGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGTTTGAAACCCCCATACATTGCCGTATA‑CACA‑C‑TTTTCCCGCGGGCTCCTTccg                                                                                                   >  2:1042794/1‑149 (MQ=17)                                                     cGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATA‑CACA‑C‑TTTCCAGGCGTGCTCCTTCAGCCAc                                                                                               <  2:480857/151‑1 (MQ=255)                                                     cGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATA‑CACA‑C‑TTTCCAGGCGTGCTCCTTCAGCCAc                                                                                               <  1:955218/151‑1 (MQ=255)                                                     cGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGAGGGGGGGGGTTGGAACCCCC‑CTACGTTGCCGTATA‑CACA‑C‑TTTTCCGGCGGGCGCCtttctcc                                                                                                 >  1:147204/1‑144 (MQ=14)                                                            tctcATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGTTTGAAACCCCGCTACGGTTCCGTATA‑CACA‑C‑TTTTCAGGCGTGCTCCTTCAGCCCCTCgg                                                                                           >  2:389950/1‑148 (MQ=17)                                                               cATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATA‑CACA‑C‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGACAcc                                                                                      <  2:1058660/150‑1 (MQ=255)                                                                aTCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGG‑GGGGGGGGTTTGAAACCCCCGTAAGTTTCCGTCTA‑CCCA‑C‑CTTCCCGGCGAGCTCCTTTAGCCACTCGGGCAcccc                                                                                    >  1:839885/1‑149 (MQ=17)                                                                  ccccccGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGTTCGAACCCCCCCTACGGTTCCGTATA‑CACA‑C‑TTTTCAGGCGTGCTCCTTCAGCCACCCCGACAcccc                                                                                    >  2:341397/1‑147 (MQ=17)                                                                  ccccccGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGTTCGAAACCCCCATACGTTGCCGTATA‑CACA‑C‑CTTCCCGGCGTGCTCCCTCAGCCACTCGGGCAcccc                                                                                    >  2:699595/1‑147 (MQ=17)                                                                     cccGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGTTTGAACCCCCCCTAAGTTTCCGTATA‑CACA‑C‑TTTTCAGGCGGGCTCCTTCAGCCACTCGGACACCTCA‑cc                                                                                >  2:599686/1‑149 (MQ=17)                                                                     cccGGTGTGTGCAATATACG‑AAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATA‑CACA‑C‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCA‑CCaaa                                                                             <  2:54980/151‑1 (MQ=255)                                                                      ccGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATA‑CACA‑C‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCA‑CCaaa                                                                             <  2:1106800/151‑1 (MQ=255)                                                                         gtgtgtGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGTTCGAACCCCCCGAACGTTGCCGCAAA‑CACA‑C‑TTTTCAGGCGTGGTCCTTCAGCCACTCGGCCACCCCA‑CCACAtt                                                                           >  1:876397/1‑150 (MQ=14)                                                                           gtgtgCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGAGA‑GGGGGGGGTTCGAACACCCCCTACGTTTTCGTATA‑TAAA‑C‑TTTCCCGGGGGGGGCCTTCAGCCCCTCCGACACCTCA‑CCcaa                                                                             >  1:839345/1‑146 (MQ=14)                                                                            tgtgCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGTTTGAACCCCCCATACCTTTCCCTATA‑CAAA‑C‑CTTTCCGGCGGGCTCCTTCCACCACTCGGACACCTCC‑CCAAAtttt                                                                         >  1:1104564/1‑147 (MQ=14)                                                                                     taCGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGTTTGAAACCCCCCAACGTTGCCGTATATAACC‑C‑TTTCCAGGGGGGCCCCTT‑ACCCACTCGGGCACCTCACCCAAATTATct                                                                       >  1:255845/1‑142 (MQ=2)                                                                                     taCGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGTTTGAAACCCCCATACGTTTCCGTATA‑CAAA‑C‑TTTCCAGGCGGGCTCCTTCAGCCACTCGGACACCTCA‑CCCAATTTTCGTTcc                                                                   >  2:419658/1‑146 (MQ=14)                                                                                      aCGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGG‑GGGGGGGGTTCGAACCCCCCATACGGTGCCGTATA‑CACA‑C‑TTTTCAGGCGGGCTCCTTCAGCCCCTCCGGCCCCCCC‑CCCAATTTTCGTTTCTGTCtt                                                             >  1:957893/1‑151 (MQ=14)                                                                                      aCGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATA‑CACA‑C‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCA‑CCAAATTGTCGTTCCTGTCtt                                                             <  2:545227/151‑1 (MQ=255)                                                                                         aaaaaaaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTCA‑GGGGGGGGATATGACCCCCCCTTCAATCCCGGATA‑CACA‑A‑TTTTCCGGCGTGCTCCCTCAGACACTCGGTAACCCCA‑CCAAAtttttgatcccttct                                                              >  1:147959/1‑134 (MQ=2)                                                                                          aaaaaaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATA‑CACA‑C‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCA‑CCAAATTGTCGTTCCTGTCTTGCTg                                                         <  2:870300/151‑1 (MQ=255)                                                                                          aaaaaaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATA‑CACA‑C‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCA‑CCAAATTGTCGTTCCTGTCTTGCTg                                                         <  2:415399/151‑1 (MQ=255)                                                                                           aaaaaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGG‑GGGGGGGGTTCGAACCCCCGGTACATTTTCGTATA‑CACA‑C‑TTTTCCGGCGTGCTCCTTCAGCCACCCGGGCACCCCA‑CCAAATTTTCGGTCCTTTCTTGCTgg                                                        >  1:387532/1‑151 (MQ=14)                                                                                           aaaaaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATA‑CACA‑C‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCA‑CCAAATTGTCGTTCCTGTCTTGCTgg                                                        <  1:522096/151‑1 (MQ=255)                                                                                              aaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGG‑GGGGGGGGTTCGAACCCCCCCTACGTTTCCGTATA‑CACC‑C‑TTTCCCGGGGGGCTCCCTCAGCCACCCCGGCACCTCC‑CCAAATTTTCGTTCCTGTCTTGCTggga                                                      >  1:105643/1‑148 (MQ=14)                                                                                              aaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATA‑CACA‑C‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCA‑CCAAATTGTCGTTCCTGTCTTGCTGGAAc                                                     <  1:450423/151‑1 (MQ=255)                                                                                               aaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATA‑CACA‑C‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCA‑CCAAATTGTCGTTCCTGTCTTGCTGGAACg                                                    <  2:137913/151‑1 (MQ=255)                                                                                                     gTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATA‑CACA‑C‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCA‑CCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCt                                              <  1:462642/151‑1 (MQ=255)                                                                                                          ttCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGGTTTGAACCCCCGATACGTTGCCGTATA‑AACA‑C‑TTTCCCGGCGTGCTCCTTCAGCCACCCGGACACCCCC‑CCCAAATTTCGTTCCTGTCTTGCTTGGACGGGCGCTAAatt                                         >  2:279353/1‑151 (MQ=14)                                                                                                             gTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATA‑CACA‑C‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCA‑CCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAgg                                      <  1:389878/151‑1 (MQ=255)                                                                                                                cGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATA‑CACA‑C‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCA‑CCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGaa                                   <  2:740155/151‑1 (MQ=255)                                                                                                                        tcttTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATA‑CACA‑C‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCA‑CCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGa                            <  2:1084068/150‑1 (MQ=255)                                                                                                                            tAAATATGGCGGTGA‑GGGGGGGGGTTGCACCCCCCGCAAGCTTCCGGATT‑CAAA‑CATTTCCA‑GCGGGGTCCTTCCATCACCCCGGCACCTCC‑CCAAAATTTCCGTCCTTTGTTTCTGGGACGGGCGCCCATTTACGGAAATCATTAcc                          >  1:327236/1‑148 (MQ=255)                                                                                                                            tAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATA‑CACA‑C‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCA‑CCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTg                        <  2:224893/150‑1 (MQ=255)                                                                                                                              aaTATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATA‑CACA‑C‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCA‑CCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAgg                     <  2:18708/151‑1 (MQ=255)                                                                                                                               atatGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATA‑CACA‑C‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCA‑CCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGt                    <  2:969898/151‑1 (MQ=255)                                                                                                                                 atGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATA‑CACA‑C‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCA‑CCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAgg                     >  2:700010/1‑148 (MQ=255)                                                                                                                                 atGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATA‑CACA‑C‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCA‑CCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTca                  <  1:630007/151‑1 (MQ=255)                                                                                                                                 atGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATA‑CACA‑C‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCA‑CCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTc                   <  2:1064382/150‑1 (MQ=255)                                                                                                                                   ggCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATA‑CACA‑C‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCA‑CCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTcaac                >  2:913974/1‑151 (MQ=255)                                                                                                                                       gTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATA‑CACA‑C‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCA‑CCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAAc            <  1:678455/151‑1 (MQ=255)                                                                                                                                       gTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATA‑CACA‑C‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCA‑CCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAAc            <  1:789546/151‑1 (MQ=255)                                                                                                                                         gA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATA‑CACA‑C‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCA‑CCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACtt          <  2:723127/151‑1 (MQ=255)                                                                                                                                         gA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATA‑CACA‑C‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCA‑CCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACtt          >  2:383197/1‑151 (MQ=255)                                                                                                                                         gA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATA‑CACA‑C‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCA‑CCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACt           <  2:503293/150‑1 (MQ=255)                                                                                                                                         gA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATA‑CACA‑C‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCA‑CCAAATTGACGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACtt          <  1:524312/151‑1 (MQ=255)                                                                                                                                                  gATTCGAACCCCCGATACGTTGCCGTATA‑CACA‑C‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCA‑CCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGaaaa  <  2:502799/151‑1 (MQ=255)                                                                                                                                                   aTTCGAACCCCCGATACGTTGCCGTATA‑CACA‑C‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCA‑CCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGaaaa  >  2:442094/1‑150 (MQ=255)                                                                                                                                                   |                                                                                                                                                          CACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGGGGGGATTCGAACCCCCGATACGTTGCCGTATA‑CACA‑C‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCA‑CCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAA  >  NC_000913/1097436‑1097730

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑