Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913993,9170AC41.2% 91.0 / 10.3 52V43G (GTG→GGG) ssuBaliphatic sulfonate ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base A (24/6);  new base C (0/21);  total (24/28)
Fisher's exact test for biased strand distribution p-value = 5.11e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGCCTCCTTCAAATGAT  >  NC_000913/993767‑994066
                                                                                                                                                          |                                                                                                                                                     
ggTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCcca                                                                                                                                                       <  2:326550/151‑1 (MQ=255)
   aGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCccacc                                                                                                                                                     >  2:590266/1‑150 (MQ=255)
     cAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCaccacc                                                                                                                                                  >  2:973174/1‑151 (MQ=255)
     cAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCaccacc                                                                                                                                                  >  2:357138/1‑151 (MQ=255)
     cAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCaccacc                                                                                                                                                  <  1:200846/151‑1 (MQ=255)
     cAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCaccacc                                                                                                                                                  >  1:445775/1‑151 (MQ=255)
       gACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGc                                                                                                                                                <  1:316863/151‑1 (MQ=255)
                ccTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTg                                                                                                                                       >  2:1040309/1‑151 (MQ=255)
                   gAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCGCCGCCACAAACTGAcc                                                                                                                                    >  2:138105/1‑151 (MQ=255)
                   gAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGAcc                                                                                                                                    >  2:981559/1‑151 (MQ=255)
                    aaccatcttCCGCGTTTCTTCCTGATTTCCGCCGGGCGGGTGGGTCCCGGAAAACACCCCGCTGGGGGTGGGCTTTCCCAGCCCTCCCAGCAGGCGCGGCAGGTTCTTTTTCCCCCCCCCGCGGCGGCCCACCACCGCCACAAACTGa                                                                                                                                      <  1:803540/145‑1 (MQ=255)
                     aaCATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTg                                                                                                                                  >  1:40102/1‑151 (MQ=255)
                          catTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCgg                                                                                                                              >  2:356591/1‑150 (MQ=255)
                               gctgttctcCCTAAATTCCAGCCGGGGGGGGGGCCCCCGAAACCCCACCGCCGGCGTTGGGCTTTCCCACCCCGCCCGGCAGGCGCAGAGGGGTCCTTTTCCCCCCCCCGCGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAatat                                                                                                                         <  1:813951/145‑1 (MQ=255)
                               gcgTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATg                                                                                                                        >  2:374192/1‑151 (MQ=255)
                                cgTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGt                                                                                                                       >  2:171784/1‑151 (MQ=255)
                                 gtgtCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGt                                                                                                                       >  1:640021/1‑150 (MQ=255)
                                  tgtCTTCCTGATTTTCACCCGGGGGTGGGGTGCCCGCAAACACACCGCCTGCGTTTGGCTTTCCCAGCCCTGCCAGCGGGCGCAGCAGGGTATTTTTCCCCCCCCCGCGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTa                                                                                                                      <  2:1039566/150‑1 (MQ=255)
                                   gtCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTaa                                                                                                                     >  1:133041/1‑150 (MQ=255)
                                    tCTTCCTAATTTTGAGCCGGGGGTGTGGTCCCGGCAAACACCCCGCCGGCGTTTGGCTTTCCCAGCCCTCCCAGCGGGCGCAGCAGGGTCTTTTGCCCCCCCCCGCTGCGGCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTaaa                                                                                                                    <  2:618960/150‑1 (MQ=255)
                                     tttCCCGAATTTCCAGCCGGGGGTGGGGCCCCCCAAAACCCACCGCTTGGGGTTGGCTTTTCCAGCCCTCCCAGCGGGCGCAGCGGGTTCCTTTTCCCCCCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCgg                                                                                                                              <  1:923890/139‑1 (MQ=255)
                                       cccTGATTTCCAGCCGGGGGTGGGGTCCCCGCTAACACACCGCGTGCGGTGGGCTTTCCCAGCCCTGCCGGCAGCCGCAGCGGGGTTTTTTTCCCCCCCCCGCTGCGGCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTaaa                                                                                                                    <  1:615048/146‑1 (MQ=255)
                                         cTGAATGTCAGCCAGCGTTGTGGGGCCGTTAAACTCATCGCCTGAGGTTGGCTTTCCCAGTCCTGCCAGCCGGAGCAGCAGGGTTTTTTCGCCACCCCCGCTGCGGCCCTCCCCCGCACAAACTGGCCTGCCGGAATATGTAAATCCAg                                                                                                               <  1:402614/149‑1 (MQ=255)
                                          tGATTTTCACCCGGGGGTGTGGCCCCCGCTAACCCACCGCCTGCGTTTGGCTTTCCCAGCCCTGCCAGGAGGCGCAGCGGGGTCTTTTTCCCCCCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGt                                                                                                              <  1:1097697/150‑1 (MQ=255)
                                                     ccagcGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGAc                                                                                                   >  1:820645/1‑151 (MQ=255)
                                                      cagcGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACg                                                                                                  >  1:709927/1‑151 (MQ=255)
                                                         gcGGTGGGGCGCCCGCAACCACACCGCCTGGGGTGGGCTTTCCCGGCCCCGCCAGCAGGCGCAGAAGGTTTCTTTCCCCCCCCCCGCTGCGCCCCCCCGCCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGat                                                                                                <  2:415715/151‑1 (MQ=255)
                                                              gtggtgCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATAtttt                                                                                           >  2:743747/1‑151 (MQ=255)
                                                                ggtgCCCGCTACCACACCGCCGGGGGTTGGCGTTCCCAGCCCTGCCGGCAGGCGCAGCGGGGTCTTTTTCCCCCCCCCGCTGCGGCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTc                                                                                          <  2:312236/150‑1 (MQ=255)
                                                                         tAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGt                                                                                >  2:480498/1‑151 (MQ=255)
                                                                          aaCACACCGCCTGGGGTTGGCTTTCCCAGCCCTGCCGGCGGGCGCAGCGGGGTATTTTGCCCCCCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAAt                                                                                  <  2:806074/148‑1 (MQ=255)
                                                                          aaCACACCGCCTGCGTTTGGCTTTTCCAGCCCTGCCAGCAGGCGCAGCAGGGTTTTTTTCCCCCCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAAt                                                                                  <  2:699254/148‑1 (MQ=255)
                                                                          aaCACACCGCCGGGGGTGGGCTTTCCCAGCCCTGCCAGCGGGCGCAGCGGGGTATTTTTCCCCCCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAAt                                                                                  <  2:337791/148‑1 (MQ=255)
                                                                            cacaTCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTg                                                                             >  1:147045/1‑151 (MQ=255)
                                                                              caTCGCCGGCGTTGGGCTTTCCCAGCCCTGCCAGCGGGCGCAGCAGGTTTCTTTTCCCCCCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGc                                                                            <  2:110016/150‑1 (MQ=255)
                                                                              caTCCCCTGCGTTTGGCTTTCCCAGCCCTGCCAGCAGGCGCGGCAGGGTATTTTTGCCCCCCCCGCTGGGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTg                                                                             <  2:90125/149‑1 (MQ=255)
                                                                               ccccctgggggTTGGCTTTCCCAGCCCGCCCAGGCGGCGCAGGAGGTTCCTTTTGCCACCACCGCTGCGGCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCtt                                                                          <  1:994868/142‑1 (MQ=255)
                                                                               aTCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCtt                                                                          >  2:246640/1‑151 (MQ=255)
                                                                                      gcgttgggCGTTCCCACCCCGCCCAGCAGGCCCAGCAGGTTCTTTTTGCCCCCCCCGCGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCtt                                                                          <  1:426489/138‑1 (MQ=255)
                                                                                        ggTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTg                                                                 >  2:743579/1‑151 (MQ=255)
                                                                                                ttcccagcccccccAGCAGCCGCGGCGGGTTCTTTTTGCCCCCCCCGCGGCGCCCCCCCCCCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCa                                                                    <  1:638681/128‑1 (MQ=255)
                                                                                                  cccAGCCCTGCCAGCGGGCGCGGCGGGGTTTTTTTGCCCCCACCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGcaacaa                                                         <  1:120827/148‑1 (MQ=255)
                                                                                                        ccTGCCAGGAGGCGAAGCAGTGTTTTTTTCCCACCCCCCCCGCGCCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGt                                                   <  1:656012/149‑1 (MQ=255)
                                                                                                             cagcggCCGCGGCGGGGTACTTTACCCCCCCCCGCGGCGCCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGccc                                               <  2:926719/148‑1 (MQ=255)
                                                                                                                           ggtttcTTTTCCCCCCCCCGCGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAg                                  <  2:459510/142‑1 (MQ=255)
                                                                                                                             gTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTAt                            >  2:648505/1‑151 (MQ=255)
                                                                                                                              tttttttGCCCCCCCCGGCGCCGCCCCCCCCCCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGc                                 <  1:734351/143‑1 (MQ=255)
                                                                                                                                     gCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCg                    >  2:344758/1‑151 (MQ=255)
                                                                                                                                      cccccccccGCGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCg                    <  1:722697/144‑1 (MQ=255)
                                                                                                                                        cccccccGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTg                                                                             <  2:810144/87‑1 (MQ=255)
                                                                                                                                        caccacCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTg                                                                             >  1:810020/1‑92 (MQ=255)
                                                                                                                                           caccGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGcc               >  1:240586/1‑151 (MQ=255)
                                                                                                                                            accGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGcc               >  2:226950/1‑150 (MQ=255)
                                                                                                                                             ccGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGcctc             >  1:273893/1‑151 (MQ=255)
                                                                                                                                              cagagagcccccACAGCCGCCCCAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGcct              <  1:304384/141‑1 (MQ=255)
                                                                                                                                                    ggCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGCCTCCTTCaa       >  2:88215/1‑150 (MQ=255)
                                                                                                                                                     gCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGCCTCCTTCAAAt     >  2:291657/1‑151 (MQ=255)
                                                                                                                                                      cccccccACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGCCTCCTTCAAAt     <  1:712823/150‑1 (MQ=255)
                                                                                                                                                       cccACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGcctcc            <  2:445864/143‑1 (MQ=255)
                                                                                                                                                         caccacCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGCCTCCTTCAAATGAt  >  2:359458/1‑151 (MQ=255)
                                                                                                                                                          |                                                                                                                                                     
GGTAGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGCCTCCTTCAAATGAT  >  NC_000913/993767‑994066

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: