Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 847,444 | 0 | G | T | 44.6% | 72.0 / 34.4 | 56 | I187I (ATC→ATA) | glnH | glutamine transporter subunit |
Reads supporting (aligned to +/- strand): ref base G (5/26); new base T (25/0); total (30/26) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 2.73e-11 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTCACGCAGCTCGTCGCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGGTTTTGATGAAGTACAGAATGTTTGGCGTATCGTGCAGAACGGCGTCTGCGCGGTTGGTGCCCAGTTCCATATAGGCGTTATCGATGTTCGGGAACTGACGCAGATCTTTAGTTTTGATGTTTGCTTTCGCGTAATCAACGGAGCCAGTACCG > NC_000913/847299‑847591 | tGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTCACGCAGCTCGTCGCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGGTTTtgatga < 2:336063/150‑1 (MQ=255) gTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTCACGCAGCTCGTCGCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGGTTTTGATGaa < 1:950274/150‑1 (MQ=255) gTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTCACGCAGCTCGTCGCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGGTTTTTTTGAAGTAc > 2:6431/1‑150 (MQ=255) ttCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTCACGCAGCTCGTCGCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGGTTTTTTTGAAGGACAGaa > 2:973567/1‑148 (MQ=255) tctcGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTCACGCAGCTCGTCGCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGGTTTTTTTGAAGGACAGAAtagt > 1:356765/1‑148 (MQ=255) tctcGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTCACGCAGCTCGTCGCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGGTTTTTATGGAGTACAGAATGtt > 1:541080/1‑151 (MQ=255) cgcgCAGGGTTTTCAACGCGCCGTTGACTTTGTCACGCAGCTCGTCGCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGGTTTTTTTGAAGGAAAAAAAGttt > 1:923468/1‑149 (MQ=255) cgcAGGGTTTTCAACGCGCCGTTGACTTTGTCACGCAGCTCGTCGCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGGGTTTTTTGAAGTAAAGAAttgtt > 1:41781/1‑143 (MQ=255) aGGGTTTTCAACGCGCCGTTGACTTTGTCACGCAGCTCGTCGCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGGGTTTTTTGAAGTACAGAAAGttt > 2:164380/1‑144 (MQ=255) gggTTTTCAACGCGCCGTTGACTTTGTCACGCAGCTCGTCGCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGGTTTTGATGAAGTACAGAATGTTTGGCGTATc < 1:716570/151‑1 (MQ=255) ttttCAACGCGCCGTTGACTTTGTCACGCAGCTCGTCGCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGGTTTTTGTTAAGTACAGAATGttttggggattgt > 2:460956/1‑140 (MQ=255) ttttCAACGCGCCGTTGACTTTGTCACGCAGCTCGTCGCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGGTTTTTGTGAAGTACATAAAGTTTGGGGGATAGTg > 2:1061750/1‑151 (MQ=255) tttCAACGCGCCGTTGACTTTGTCACGCAGCTCGTCGCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGGTTTTGATGAAGTACAGAATGTTTGGCGTATCGTg < 2:950320/150‑1 (MQ=255) cAACGCGCCGTTGACTTTGTCACGCAGCTCGTCGCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGGTTTTGATGAAGTACAGAATGTTTGGCGTATCGTGCAg < 1:883329/150‑1 (MQ=255) aCGCGCCGTTGACTTTGTCACGCAGCTCGTCGCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGGTTTTTATGAAGGAAAGAATGTTTTGCGGATCGTGCAGaa > 2:256032/1‑150 (MQ=255) cgcgCCGTTGACTTTGTCACGCAGCTCGTCGCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGGTTTTGATGAAGTACAGAATGTTTGGCGTATCGTGCAGAACg < 1:613791/151‑1 (MQ=255) gcCGTTGACTTTGTCACGCAGCTCGTCGCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGGTTTTGATGAAGTACAGAATGTTTGGCGTATCGTGCAGAACGGCg < 2:790726/151‑1 (MQ=255) gTTGACTTTGTCACGCAGCTCGTCGCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGGTTTTTTTGAAGGAAAGAAAGTTTTGGGGATCGTGCAGaa > 1:994348/1‑143 (MQ=255) gTTGACTTTGTCACGCAGCTCGTCGCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGGTTTTTGTGAAGGAAAAAATGTTTTGCCTATTCTGCAggaacg > 1:81008/1‑141 (MQ=255) gTTGACTTTGTCACGCAGCTCGTCGCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGGTTTTGATGAAGTACAGAATGTTTGGCGTATCGTGCAGAACGGCGTCt < 1:950942/151‑1 (MQ=255) tGACTTTGTCACGCAGCTCGTCGCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGGTTTTTTTGAAGTACAGAATGTTTTGGGGATCGTGCAGAAAGGCGTCTg > 2:1078241/1‑150 (MQ=255) cGCAGCTCGTCGCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGGTTTTGATGAAGTACAGAATGTTTGGCGTATCGTGCAGAACGGCGTCTGCGCGGTTGGTGc < 1:975038/151‑1 (MQ=255) gCTCGTCGCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGGTTTTTTTTAAGTACAGAATGTTTTGCGGATCGTGGAGAAACGGGCCTGCGCGGGTgggggcc > 1:497180/1‑144 (MQ=255) cTCGTCGCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGGTTTTTTTGAAGTACAGCATGTTTGGCGGGTCTTGCCGAACGGGGTTTGCGCGGTTGGTGccccct > 1:10006/1‑148 (MQ=255) cTCGTCGCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGGATTTGATTAAGTATAGAAAAGTTTGCGTAACGTGCCAGACGGCGGCTGCGCGGgtggtgggcag > 1:744929/1‑145 (MQ=255) gtcgCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGGTTTTTTTGAAGTAAAGAATTTTTGGCGGATCGTGCAGAAACGCGGCTGCGCGGGTGGGGCCCCGtttc > 1:245389/1‑149 (MQ=255) gtcgCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGGTTTTTATGAAGGACAGAATGTTTTGCGGATCGTGCAGAACGGCGGCTGCGCGGGTGGTGCCCAGGTcc > 2:991380/1‑151 (MQ=255) tACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGGTTTTTTTGAAGGAAAGAAAGTTTTGGGTATCGTGCAGAAAGGCGGCTTCGCGGGTGGGGGCCAGtttcct > 2:511976/1‑144 (MQ=255) ccTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGGTTTTGATGAAGTACAGAATGTTTGGCGTATCGTGCAGAACGGCGTCTGCGCGGTTGGTGCCCAGTTCCATATAgg < 2:798635/151‑1 (MQ=255) tttCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGGTTTTTTTTAAGTAAAGAATGTTTGGGGTATCGTGCAGAACGGGCGCTGCGCGGGTGGGGcccccgtccc > 1:209724/1‑136 (MQ=255) ttCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGGTTTTGATGAAGTACAGAATGTTTGGCGTATCGTGCAGAACGGCGTCTGCGCGGTTGGTGCCCAGTTCCATATAGGc < 1:719048/149‑1 (MQ=255) cGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGGTTTTGATGAAGTACAGAATGTTTGGCGTATCGTGCAGAACGGCGTCTGCGCGGTTGGTGCCCAGTTCCATATAGGCGTTATCGATg < 2:509854/151‑1 (MQ=255) gTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGGTTTTTATGAAGAAAAAAATGTTTTGCGGATCGTGCAGAAAGGGGTCTGCGCGGGTGGGGCCCCGGTCCCTATAGGCGTTATCGAAGTTCgggga > 2:1069103/1‑148 (MQ=255) cgcTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGGTTTTGTTGAAGTAAAGAATGTTTTGCGGATCGTGCAGAACGGCGTCTGCGCGGGTGGTGCCCCGTTCCATTTAGGCGGTATCGATGTTTGGGGAATGACg > 1:420195/1‑147 (MQ=255) cgcTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGGTTTTGATGAAGTACAGAATGTTTGGCGTATCGTGCAGAACGGCGTCTGCGCGGTTGGTGCCCAGTTCCATATAGGCGTTATCGATGTTCGGGAACTGACGCa < 2:116199/149‑1 (MQ=255) gcTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGGTTTTGCTGAAGGAAAGAAAAATTTGCGGATTGTGGAGAACGGCGGCTTCGCGGTTGGGGGCCAGTTCCACAAAAGCGTTTTTGTAGTTTGGGGACTg > 2:907259/1‑143 (MQ=255) cTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGGTTTTTATGAAGCACAGAAAGTTTTGCGGGTCGGGCCGAAAGGCGGCTGCGCGGGTGGTGGCCACTTTCCTATATGCGGTATCCATGTTCGGGGACTg > 1:853157/1‑142 (MQ=255) ccAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGGTTTTGATGAAGTACAGAATGTTTGGCGTATCGTGCAGAACGGCGTCTGCGCGGTTGGTGCCCAGTTCCATATAGGCGTTATCGATGTTCGGGAACTGACGCAGATCtt < 1:475145/150‑1 (MQ=255) agagagTCACCTACCGCTTTGAACTGACCGTTACCGGCGGTTTTGATGAAGTACAGAATGTTTGGCGTATCGTGCAGAACGGCGTCTGCGCGGTTGGTGCCCAGTTCCATATAGGCGTTATCGATGTTCGGGAACTGACGCAGATCTTTAg < 2:617098/151‑1 (MQ=255) gagagTCACCTACCGCTTTGAACTGACCGTTACCGGCGGTTTTGATGAAGTACAGAATGTTTGGCGTATCGTGCAGAGCGGCGTCTGCGCGGTTGGTGCCCAGTTCCATATAGGCGTTATCGATGTTCGGGAACTGACGCAGATCTTTAg < 1:7382/150‑1 (MQ=255) gagTCACCTACCGCTTTGAACTGACCGTTACCGGCGGTTTTGATGAAGTACAGAATGTTTGGCGTATCGTGCAGAACGGCGTCTGCGCGGTTGGTGCCCAGTTCCATATAGGCGTTATCGATGTTCGGGAACTGACGCAGATCTTTAGttt < 2:368459/151‑1 (MQ=255) agTCACCTACCGCTTTGAACTGACCGTTACCGGCGGTTTTTTTGAAGGACAAAAATTTTTGCGTATCGGGCAGAACGGCGTCTGCGCGGGTGGGGCCCCGGTCCCTATAAGGGGTATTGATGGTCGGGAAATGACGCCAATCTTTTGtttt > 2:747605/1‑151 (MQ=255) cACCTACCGCTTTGAACTGACCGTTACCGGCGGTTTTGATGAAGTACAGAATGTTTGGCGTATCGTGCAGAACGGCGTCTGCGCGGTTGGTGCCCAGTTCCATATAGGCGTTATCGATGTTCGGGAACTGACGCAGATCTTTAGTTTTGAt < 2:171636/151‑1 (MQ=255) cACCTACCGCTTTGAACTGACCGTTACCGGCGGTTTTGATGAAGTACAGAATGTTTGGCGTATCGTGCAGAACGGCGTCTGCGCGGTTGGTGCCCAGTTCCATATAGGCGTTATCGATGTTCGGGAACTGACGCAGATCTTTAGTTTTGAt < 2:90260/151‑1 (MQ=255) aCCTACCGCTTTGAACTGACCGTTACCGGCGGTTTTGATGTAGTACAAGATGTTTTGCGGATCGTGCAGAACGGCGTCCGCGGGGGTGGGGCCCCGTTCCCTATAAGGGGTTTCGATGTTCGGGGACTGACGCAGATCTTTTGttttttt > 1:666300/1‑147 (MQ=255) tACCGCTTTGAACTGACCGTTACCGGCGGTTTTTGTGAAGGAAAGAAAGTTTTGCGGATCGTGCAGAACGGGGGCTGCGCGGGTGGGGCCCCGTTCCCTATAGGCGGTTTCGATGTTTGGGGAATGACGCAGGTCttttgtttttttgttt > 2:893707/1‑151 (MQ=255) aCCGCTTTGAACTGACCGTTACCGGCGGTTTTGATGAAGTACAGAATGTTTGGCGTATCGTGCAGAACGGCGTCTGCGCGGTTGGTGCCCAGTTCCATATAGGCGTTATCGATGTTCGGGAACTGACGCAGATCTTTAGTTTTGATGTTTg < 2:565283/151‑1 (MQ=255) cGCTTTGAACTGACCGTTACCGGCGGTTTTTTTGAAGTACAAAATGTTTTGCGGATCCTGGAGGACGGCGTCTGCGCGGGTGGTGCCCAGTTCCATATAGGGGTTATCGATGTTCGGGGACTGGCGCAGAACTTTTTTTTTGATGTTTGCt > 2:852400/1‑151 (MQ=255) gcgttGAACTGACCGTTACCGGCGGTTTTGATGAAGTACAGAATGTTTGGCGTATCGTGCAGAACGGCGTCTGCGCGGTTGGTGCCCAGTTCCATATAGGCGTTATCGATGTTCGGGAACTGACGCAGATCTTTAGTTTTGATGTTTGCtt < 2:492193/148‑1 (MQ=255) tGACCGTTACCGGCGGTTTTTTTGAAGGACAGAAAGGTTTGCGTGGTGGGCAGGACGGCGTCTGCTCGGGTGGGGACCAACTTCCTATTGGCGGTTTCGGTGTTTCGGGACTGACCCAGAAACTTTGTTTTTTTGTTTGCTTTCGCGTAAt > 1:133660/1‑151 (MQ=255) tACCGGCGGTTTTGATGAAGTACATAATGTTTGGCGTATCGTGCAGAACGGCGTCTGCGCGGTTGGTGCCCAGTTCCATATAGGCGTTATCGATGTTCGGGAACTGACGCAGATCTTTAGTTTTGATGTTTGCTTTCGCGTAATCAACgg < 1:423698/150‑1 (MQ=255) aCCGGCGGTTTTGATGAAGTACAGAATGTTTGGCGTATCGTGCAGAACGGCGTCTGCGCGGTTGGTGCCCAGTTCCATATAGGCGTTATCGATGTTCGGGAACTGACGCAGATCTTTAGTTTTGATGTTTGCTTTCGCGTAATCAACGGa < 2:527439/150‑1 (MQ=255) aCCGGCGGTTTTGATGAAGTACAGAATGTTTGGCGTATCGTGCAGAACGGCGTCTGCGCGGTTGGTGCCCAGTTCCATATAGGCGTTATCGATGTTCGGGAACTGACGCAGATCTTTAGTTTTGATGTTTGCTTTCGCGTAATCAACGGAg < 1:255975/151‑1 (MQ=255) aCCGGCGGTTTTGATGAAGTACAGAATGTTTGGCGTATCGTGCAGAACGGCGTCTGCGCGGTTGGTGCCCAGTTCCATATAGGCGTTATCGATGTTCGGGAACTGACGCAGATCTTTAGTTTTGATGTTTGCTTTCGCGTAATCAACGGAg < 1:13707/151‑1 (MQ=255) ggcggTTTTGATGAAGTACAGAATGTTTGGCGTATCGTGCAGAACGGCGTCTGCGCGGTTGGTGCCCAGTTCCATATAGGCGTTATCGATGTTCGGGAACTGACGCAGATCTTTAGTTTTGATGTTTGCTTTCGCGTAATCAACGGAGCCa < 2:824084/151‑1 (MQ=255) cggTTTTGATGAAGTACAGAATGTTTGGCGTATCGTGCAGAACGGCGTCTGCGCGGTTGGTGCCCAGTTCCATATATGCGTTATCGATGTTCGGGATCTGACGCAGATCGTTAGTTTTGATGTTTGCTTTCGCGTAATCAACGGAGCCAg < 1:846387/150‑1 (MQ=255) tttGATGAAGTACAGAATGTTTGGCGTATCGTGCAGAACGGCGTCTGCGCGGTTGGTGCCCAGTTCCATATAGGCGTTATCGATGTTCGGGAACTGACGCAGATCTTTAGTTTTGATGTTTGCTTTCGCGTAATCAACGGAGCCAGTACCg > 2:1052599/1‑151 (MQ=255) | TGTAAGTTCCGTTCTCGCGCAGGGTTTTCAACGCGCCGTTGACTTTGTCACGCAGCTCGTCGCTACCTTTCGGGAACGCAATACCGTATTGCTGCGCTTCCAGAGAGTCACCTACCGCTTTGAACTGACCGTTACCGGCGGTTTTGATGAAGTACAGAATGTTTGGCGTATCGTGCAGAACGGCGTCTGCGCGGTTGGTGCCCAGTTCCATATAGGCGTTATCGATGTTCGGGAACTGACGCAGATCTTTAGTTTTGATGTTTGCTTTCGCGTAATCAACGGAGCCAGTACCG > NC_000913/847299‑847591 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |