Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,603,494	C→A	C6* (TGC→TGA)	yjjQ →	putative transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,603,494	0	C	A	98.8%	307.9 / NA	80	C6* (TGC→TGA)	yjjQ	putative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base A (43/36);  total (43/37)
Fisher's exact test for biased strand distribution p-value = 4.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGTATGCTGATATGAAAGTGAATGCTAAGGATAATTTATTCGCTTAATCTATTAATTTGCTGGAAACATTTAAGGAATGAATATCCTGAATAAACCATCATAATTTTTAGACAGTGGATGTGGAGGAAATATGTTGCCAGGATGCTGCAAAAATGGAATTGTTATCAGTAAAATACCTGTTATGCAAGCAGGGTTAAAAGAGGTCATGAGGACTCACTTCCCTGAATATGAAATAATATCCAGCGCCTCTGCGGAGGACCTTACCTTATTACAATTACGTCGTTCCGGATTAGT  >  NC_000913/4603347‑4603640                                                                                                                                                    |                                                                                                                                                   aGTATGCTGATATGAAAGTGAATGCTAAGGATAATTTATTCGCTTAATCTATTAATTTGCTGGAAACATTTAAGGAATGAATATCCTGAATAAACCATCATAATTTTTAGACAGTGGATGTGGAGGAAATATGTTGCCAGGATGCTGaaaa                                                                                                                                                 <  2:346336/151‑1 (MQ=255) aGTATGCTGATATGAAAGTGAATGCTAAGGATAATTTATTCGCTTAATCTATTAATTTGCTGGAAACATTTAAGGAATGAATATCCTGAATAAACCATCATAATTTTTAGACAGTGGATGTGGAGGAAATATGTTGCCAGGATGCTGaaa                                                                                                                                                  >  2:33173/1‑150 (MQ=255)       cTGATATGAAAGTGAATGCTAAGGATAATTTATTCGCTTAATCTATTAATTTGCTGGAAACATTTAAGGAATGAATATCCTGAATAAACCATCATAATTTTTAGACAGTGGATGTGGAGGAAATATGTTGCCAGGATGCTGAAAAAATgg                                                                                                                                            >  2:836445/1‑150 (MQ=255)            atGAAAGTGAATGCTAAGGATAATTTATTCGCTTAATCTATTAATTTGCTGGAAACATTTAAGGAATGAATATCCTGAATAAACCATCATAATTTTTAGACAGTGGATGTGGAGGAAATATGTTGCCAGGATGCTGAAAAAATGGAATTg                                                                                                                                       <  1:8940/150‑1 (MQ=255)            atGAAAGTGAATGCTAAGGATAATTTATTCGCTTAATCTATTAATTTGCTGGAAACATTTAAGGAATGAATATCCTGAATAAACCATCATAATTTTTAGACAGTGGATGTGGAGGAAATATGTTGCCAGGATGCTGAAAAAATGGAATTGt                                                                                                                                      <  2:942370/151‑1 (MQ=255)              gAAAGTGAATGCTAAGGATAATTTATTCGCTTAATCTATTAATTTGCTGGAAACATTTAAGGAATGAATATCCTGAATAAACCATCATAATTTTTAGACAGTGGATGTGGAGGAAATATGTTGCCAGGATGCTGAAAAAATGGAATTGtt                                                                                                                                     <  1:1104117/150‑1 (MQ=255)              gAAAGTGAATGCTAAGGATAATTTATTCGCTTAATCTATTAATTTGCTGGAAACATTTAAGGAATGAATATCCTGAATAAACCATCATAATTTTTAGACAGTGGATGTGGAGGAAATATGTTGCCAGGATGCTGAAAAAATGGAATTGTTa                                                                                                                                    <  2:308478/151‑1 (MQ=255)               aaaGTGAATGCTAAGGATAATTTATTCGCTTAATCTATTAATTTGCTGGAAACATTTAAGGAATGAATATCCTGAATAAACCATCATAATTTTTAGACAGTGGATGTGGAGGAAATATGTTGCCAGGATGCTGAAAAAATGGAATTGTTAt                                                                                                                                   <  2:666847/151‑1 (MQ=255)                     aaTGCTAAGGATAATTTATTCGCTTAATCTATTAATTTGCTGGAAACATTTAAGGAATGAATATCCTGAATAAACCATCATAATTTTTAGACAGTGGATGTGGAGGAAATATGTTGCCAGGATGCTGAAAAAATGGAATTGTTATCAGTa                                                                                                                              >  1:163330/1‑150 (MQ=255)                             ggATAATTTATTCGCTTAATCTATTAATTTGCTGGAAACATTTAAGGAATGAATATCCTGAATAAACCATCATAATTTTTAGACAGTGGATGTGGAGGAAATATGTTGCCAGGATGCTGAAAAAATGGAATTGTTATCAGTAAAATACCTg                                                                                                                     <  2:200120/151‑1 (MQ=255)                              gATAATTTATTCGCTTAATCTATTAATTTGCTGGAAACATTTAAGGAATGAATATCCTGAATAAACCATCATAATTTTTAGACAGTGGATGTGGAGGAAATATGTTGCCAGGATGCTGAAAAAATGGAATTGTTATCAGTAAAATACCTGt                                                                                                                    >  1:263577/1‑151 (MQ=255)                                 aaTTTATTCGCTTAATCTATTAATTTGCTGGAAACATTTAAGGAATGAATATCCTGAATAAACCATCATAATTTTTAGACAGTGGATGTGGAGGAAATATGTTGCCAGGATGCTGAAAAAATGGAATTGTTATCAGTAAAATACCTGTTa                                                                                                                  <  1:1063880/150‑1 (MQ=255)                                 aaTTTATTCGCTTAATCTATTAATTTGCTGGAAACATTTAAGGAATGAATATCCTGAATAAACCATCATAATTTTTAGACAGTGGATGTGGAGGAAATATGTTGCCAGGATGCTGAAAAAATGGAATTGTTATCAGTAAAATACCTGTTAt                                                                                                                 <  2:351772/151‑1 (MQ=255)                                 aaTTTATTCGCTTAATCTATTAATTTGCTGGAAACATTTAAGGAATGAATATCCTGAATAAACCATCATAATTTTTAGACAGTGGATGTGGAGGAAATATGTTGCCAGGATGCTGAAAAAATGGAATTGTTATCAGTAAAATACCTGTTAt                                                                                                                 >  1:420748/1‑151 (MQ=255)                                  aTTTATTCGCTTAATCTATTAATTTGCTGGAAACATTTAAGGAATGAATATCCTGAATAAACCATCATAATTTTTAGACAGTGGATGTGGAGGAAATATGTTGCCAGGATGCTGAAAAAATGGAATTGTTATCAGTAAAATACCTGTTATg                                                                                                                <  2:206080/151‑1 (MQ=255)                                  aTTTATTCGCTTAATCTATTAATTTGCTGGAAACATTTAAGGAATGAATATCCTGAATAAACCATCATAATTTTTAGACAGTGGATGTGGAGGAAATATGTTGCCAGGATGCTGAAAAAATGGAATTGTTATCAGTAAAATACCTGTTATg                                                                                                                <  1:594140/151‑1 (MQ=255)                                    ttATTCGCTTAATCTATTAATTTGCTGGAAACATTTAAGGAATGAATATCCTGAATAAACCATCATAATTTTTAGACAGTGGATGTGGAGGAAATATGTTGCCAGGATGCTGAAAAAATGGAATTGTTATCAGTAAAATACCTGTTATGc                                                                                                               >  1:966963/1‑150 (MQ=255)                                     tATTCGCTTAATCTATTAATTTGCTGGAAACATTTAAGGAATGAATATCCTGAATAAACCATCATAATTTTTAGACAGTGGATGTGGAGGAAATATGTTGCCAGGATGCTGAAAAAATGGAATTGTTATCAGTAAAATACCTGTTATGCa                                                                                                              >  1:937636/1‑150 (MQ=255)                                     tATTCGCTTAATCTATTAATTTGCTGGAAACATTTAAGGAATGAATATCCTGAATAAACCATCATAATTTTTAGACAGTGGATGTGGAGGAAATATGTTGCCAGGATGCTGAAAAAATGGAATTGTTATCAGTAAAATACCTGTTATGCa                                                                                                              >  1:392183/1‑150 (MQ=255)                                      aTTCGCTTAATCTATTAATTTGCTGGAAACATTTAAGGAATGAATATCCTGAATAAACCATCATAATTTTTAGACAGTGGATGTGGAGGAAATATGTTGCCAGGATGCTGAAAAAATGGAATTGTTATCAGTAAAATACCTGTTATGCAAg                                                                                                            <  2:477298/151‑1 (MQ=255)                                       ttCGCTTAATCTATTAATTTGCTGGAAACATTTAAGGAATGAATATCCTGAATAAACCATCATAATTTTTAGACAGTGGATGTGGAGGAAATATGTTGCCAGGATGCTGAAAAAATGGAATTGTTATCAGTAAAATACCTGTTATGCAAGc                                                                                                           >  1:185288/1‑151 (MQ=255)                                        tCGCTTAATCTATTAATTTGCTGGAAACATTTAAGGAATGAATATCCTGAATAAACCATCATAATTTTTAGACAGTGGATGTGGAGGAAATATGTTGCCAGGATGCTGAAAAAATGGAATTGTTATCAGTAAAATACCTGTTATGCAAGc                                                                                                           >  2:1053318/1‑150 (MQ=255)                                          gCTTAATCTATTAATTTGCTGGAAACATTTAAGGAATGAATATCCTGAATGAACCATCATAATTTTTAGACAGTGGATGTGGAGGAAATATGTTGCCAGGATGCTGAAAAAATGGAATTGTTATCAGTAAAATACCTGTTATGCAAGCAgg                                                                                                        <  2:1503/151‑1 (MQ=255)                                          gCTTAATCTATTAATTTGCTGGAAACATTTAAGGAATGAATATCCTGAATAAACCATCATAATTTTTAGACAGTGGATGTGGAGGAAATATGTTGCCAGGATGCTGAAAAAATGGAATTGTTATCAGTAAAATACCTGTTATGCAAGCAgg                                                                                                        >  2:318386/1‑151 (MQ=255)                                              aaTCTATTAATTTGCTGGAAACATTTAAGGAATGAATATCCTGAATAAACCATCATAATTTTTAGACAGTGGATGTGGAGGAAATATGTTGCCAGGATGCTGAAAAAATGGAATTGTTATCAGTAAAATACCTGTTATGCAAGCAGGGtt                                                                                                     <  2:474121/150‑1 (MQ=255)                                              aaTCTATTAATTTGCTGGAAACATTTAAGGAATGAATATCCTGAATAAACCATCATAATTTTTAGACAGTGGATGTGGAGGAAATATGTTGCCAGGATGCTGAAAAAATGGAATTGTTATCAGTAAAATACCTGTTATGCAAGCAGGGTTa                                                                                                    <  2:353864/151‑1 (MQ=255)                                               aTCTATTAATTTGCTGGAAACATTTAAGGAATGAATATCCTGAATAAACCATCATAATTTTTAGACAGTGGATGTGGAGGAAATATGTTGCCAGGATGCTGAAAAAATGGAATTGTTATCAGTAAAATACCTGTTATGCAAGCAGGGTTa                                                                                                    >  1:784714/1‑150 (MQ=255)                                                tCTATTAATTTGCTGGAAACATTTAAGGAATGAATATCCTGAATAAACCATCATAATTTTTAGACAGTGGATGTGGAGGAAATATGTTGCCAGGATGCTGAAAAAATGGAATTGTTATCAGTAAAATACCTGTTATGCAAGCAGGGTTaa                                                                                                   >  1:201278/1‑150 (MQ=255)                                                  tATTAATTTGCTGGAAACATTTAAGGAATGAATATCCTGAATAAACCATCATAATTTTTAGACAGTGGATGTGGAGGAAATATGTTGCCAGGATGCTGAAAAAATGGAATTGTTATCAGTAAAATACCTGTTATGCAAGCAGGGTTaaa                                                                                                  >  1:972946/1‑149 (MQ=255)                                                     tAATTTGCTGGAAACATTTAAGGAATGAATATCCTGAATAAACCATCATAATTTTTAGACAGTGGATGTGGAGGAAATATGTTGCCAGGATGCTGAAAAAATGGAATTGTTATCAGTAAAATACCTGTTATGCAAGCAGGGTTAAAAGAgg                                                                                             >  2:493823/1‑151 (MQ=255)                                                       aTTTGCTGGAAACATTTAAGGAATGAATATCCTGAATAAACCATCATAATTTTTAGACAGTGGATGTGGAGGAAATATGTTGCCAGGATGCTGAAAAAATGGAATTGTTATCAGTAAAATACCTGTTATGCAAGCAGGGTTAAAAGAGGTc                                                                                           <  1:268902/151‑1 (MQ=255)                                                         ttGCTGGAAACATTTAAGGAATGAATATCCTGAATAAACCATCATAATTTTTAGACAGTGGATGTGGAGGAAATATGTTGCCAGGATGCTGAAAAAATGGAATTGTTATCAGTAAAATACCTGTTATGCAAGCAGGGTTAAAAGAGGTCAt                                                                                         >  2:756557/1‑151 (MQ=255)                                                          tGCTGGAAACATTTAAGGAATGAATATCCTGAATAAACCATCATAATTTTTAGACAGTGGATGTGGAGGAAATATGTTGCCAGGATGCTGAAAAAATGGAATTGTTATCAGTAAAATACCTGTTATGCAAGCAGGGTTAAAAGAGGTCAt                                                                                         <  1:802978/150‑1 (MQ=255)                                                           gCTGGAAACATTTAAGGAATGAATATCCTGAATAAACCATCATAATTTTTAGACAGTGGATGTGGAGGAAATATGTTGCCAGGATGCTGAAAAAATGGAATTGTTATCAGTAAAATACCTGTTATGCAAGCAGGGTTAAAAGAGGTCATGa                                                                                       <  1:268806/151‑1 (MQ=255)                                                             tGGAAACATTTAAGGAATGAATATCCTGAATAAACCATCATAATTTTTAGACAGTGGATGTGGAGGAAATATGTTGCCAGGATGCTGAAAAAATGGAATTGTTATCAGTAAAATACCTGTTATGCAAGCAGGGTTAAAAGAGGTCATGAgg                                                                                     >  1:223509/1‑151 (MQ=255)                                                              ggAAACATTTAAGGAATGAATATCCTGAATAAACCATCATAATTTTTAGACAGTGGATGTGGAGGAAATATGTTGCCAGGATGCTGAAAAAATGGAATTGTTATCAGTAAAATACCTGTTATGCAAGCAGGGTTAAAAGAGGTCATGAgg                                                                                     >  2:425666/1‑150 (MQ=255)                                                              ggAAACATTTAAGGAATGAATATCCTGAATAAACCATCATAATTTTTAGACAGTGGATGTGGAGGAAATATGTTGCCAGGATGCTGAAAAAATGGAATTGTTATCAGTAAAATACCTGTTATGCAAGCAGGGTTAAAAGAGGTCATGAGGa                                                                                    <  2:387683/151‑1 (MQ=255)                                                                aaaCATTTAAGGAATGAATATCCTGAATAAACCATCATAATTTTTAGACAGTGGATGTGGAGGAAATATGTTGCCAGGATGCTGAAAAAATGGAATTGTTATCAGTAAAATACCTGTTATGCAAGCAGGGTTAAAAGAGGTCATGAGGACt                                                                                  >  1:416446/1‑151 (MQ=255)                                                                  aCATTTAAGGAATGAATATCCTGAATAAACCATCATAATTTTTAGACAGTGGATGTGGAGGAAATATGTTGCCAGGATGCTGAAAAAATGGAATTGTTATCAGTAAAATACCTGTTATGCAAGCAGGGTTAAAAGAGGTCATGAGGACTCa                                                                                <  1:801008/151‑1 (MQ=255)                                                                   cATTTAAGGAATGAATATCCTGAATAAACCATCATAATTTTTAGACAGTGGATGTGGAGGAAATATGTTGCCAGGATGCTGAAAAAATGGAATTGTTATCAGTAAAATACCTGTTATGCAAGCAGGGTTAAAAGAGGTCATGAGGACTCAc                                                                               >  2:136202/1‑151 (MQ=255)                                                                    aTTTAAGGAATGAATATCCTGAATAAACCATCATAATTTTTAGACAGTGGATGTGGAGGAAATATGTTGCCAGGATGCTGAAAAAATGGAATTGTTATCAGTAAAATACCTGTTATGCAAGCAGGGTTAAAAGAGGTCATGAGGACTCACt                                                                              <  1:950578/151‑1 (MQ=255)                                                                      ttAAGGAATGAATATCCTGAATAAACCATCATAATTTTTAGACAGTGGATGTGGAGGAAATATGTTGCCAGGATGCTGAAAAAATGGAATTGTTATCAGTAAAATACCTGTTATGCAAGCAGGGTTAAAAGAGGTCATGAGGACTCACtt                                                                             <  2:729730/150‑1 (MQ=255)                                                                       tAAGGAATGAATATCCTGAATAAACCATCATAATTTTTAGACAGTGGATGTGGAGGAAATATGTTGCCAGGATGCTGAAAAAATGGAATTGTTATCAGTAAAATACCTGTTATGCAAGCAGGGTTAAAAGAGGTCATGAGGACTCACTTcc                                                                           >  2:314270/1‑151 (MQ=255)                                                                        aaGGAATGAATATCCTGAATAAACCATCATAATTTTTAGACAGTGGATGTGGAGGAAATATGTTGCCAGGATGCTGAAAAAATGGAATTGTTATCAGTAAAATACCTGTTATGCAAGCAGGGTTAAAAGAGGTCATGAGGACTCACTTccc                                                                          >  2:627373/1‑151 (MQ=255)                                                                        aaGGAATGAATATCCTGAATAAACCATCATAATTTTTAGACAGTGGATGTGGAGGAAATATGTTGCCAGGATGCTGAAAAAATGGAATTGTTATCAGTAAAATACCTGTTATGCAAGCAGGGTTAAAAGAGGTCATGAGGACTCACTTccc                                                                          >  1:978920/1‑151 (MQ=255)                                                                               gaatATCCTGAATAAACCATCATAATTTTTAGACAGTGGATGTGGAGGAAATATGTTGCCAGGATGCTGAAAAAATGGAATTGTTATCAGTAAAATACCTGTTATGCAAGCAGGGTTAAAAGAGGTCATGAGGACTCACTTCCCTGAatat                                                                   >  2:54564/1‑151 (MQ=255)                                                                               gaatATCCTGAATAAACCATCATAATTTTTAGACAGTGGATGTGGAGGAAATATGTTGCCAGGATGCTGAAAAAATGGAATTGTTATCAGTAAAATACCTGTTATGCAAGCAGGGTTAAAAGAGGTCATGAGGACTCACTTCCCTGAatat                                                                   >  2:600377/1‑151 (MQ=255)                                                                                 atatCCTGAATAAACCATCATAATTTTTAGACAGTGGATGTGGAGGAAATATGTTGCCAGGATGCTGAAAAAATGGAATTGTTATCAGTAAAATACCTGTTATGCAAGCAGGGTTAAAAGAGGTCATGAGGACTCACTTCCCTGAATATg                                                                  >  2:1104319/1‑150 (MQ=255)                                                                                     ccTGAATAAACCATCATAATTTTTAGACAGTGGATGTGGAGGAAATATGTTGCCAGGATGCTGAAAAAATGGAATTGTTATCAGTAAAATACCTGTTATGCAAGCAGGGTTAAAAGAGGTCATGAGGACTCACTTCCCTGAATATGAaat                                                              >  1:108775/1‑150 (MQ=255)                                                                                     ccTGAATAAACCATCATAATTTTTAGACAGTGGATGTGGAGGAAATATGTTGCCAGGATGCTGAAAAAATGGAATTGTTATCAGTAAAATACCTGTTATGCAAGCAGGGTTAAAAGAGGTCATGAGGACTCACTTCCCTGAATATGAaat                                                              <  1:1028989/150‑1 (MQ=255)                                                                                      cTGAATAAACCATCATAATTTTTAGACAGTGGATGTGGAGGAAATATGTTGCCAGGATGCTGAAAAAATGGAATTGTTATCAGTAAAATACCTGTTATGCAAGCAGGGTTAAAAGAGGTCATGAGGACTCACTTCCCTGAATATGAaata                                                             >  1:671456/1‑150 (MQ=255)                                                                                          aTAAACCATCATAATTTTTAGACAGTGGATGTGGAGGAAATATGTTGCCAGGATGCTGAAAAAATGGAATTGTTATCAGTAAAATACCTGTTATGCAAGCAGGGTTAAAAGAGGTCATGAGGACTCACTTCCCTGAATATGAAATAATATc                                                        >  2:334703/1‑151 (MQ=255)                                                                                           tAAACCATCATAATTTTTAGACAGTGGATGTGGAGGAAATATGTTGCCAGGATGCTGAAAAAATGGAATTGTTATCAGTAAAATACCTGTTATGCAAGCAGGGTTAAAAGAGGTCATGAGGACTCACTTCCCTGAATATGAAATAATATcc                                                       >  2:371682/1‑151 (MQ=255)                                                                                              aCCATCATAATTTTTAGACAGTGGATGTGGAGGAAATATGTTGCCAGGATGCTGAAAAAATGGAATTGTTATCAGTAAAATACCTGTTATGCAAGCAGGGTTAAAAGAGGTCATGAGGACTCACTTCCCTGAATATGAAATAATATCCAgc                                                    >  2:1003851/1‑151 (MQ=255)                                                                                               ccATCATAATTTTTAGACAGTGGATGTGGAGGAAATATGTTGCCAGGATGCTGAAAAAATGGAATTGTTATCAGTAAAATACCTGTTATGCAAGCAGGGTTAAAAGAGGTCATGAGGACTCACTTCCCTGAATATGAAATAATATCCAgcg                                                   >  2:421382/1‑151 (MQ=255)                                                                                                catcatAATTTTTAGACAGTGGATGTGGAGGAAATATGTTGCCAGGATGCTGAAAAAATGGAATTGTTATCAGTAAAATACCTGTTATGCAAGCAGGGTTAAAAGAGGTCATGAGGACTCACTTCCCTGAATATGAAATAATATCCAgcgc                                                  >  1:506108/1‑151 (MQ=255)                                                                                                    atAATTTTTAGACAGTGGATGTGGAGGAAATATGTTGCCAGGATGCTGAAAAAATGGAATTGTTATCAGTAAAATACCTGTTATGCAAGCAGGGTTAAAAGAGGTCATGAGGACTCACTTCCCTGAATATGAAATAATATCCAGCGCctct                                              >  2:187519/1‑151 (MQ=255)                                                                                                    atAATTTTTAGACAGTGGATGTGGAGGAAATATGTTGCCAGGATGCTGAAAAAATGGAATTGTTATCAGTAAAATACCTGTTATGCAAGCAGGGTTAAAAGAGGTCATGAGGACTCACTTCCCTGAATATGAAATAATATCCAGCGCctct                                              >  1:731867/1‑151 (MQ=255)                                                                                                     tAATTTTTAGACAGTGGATGTGGAGGAAATATGTTGCCAGGATGCTGAAAAAATGGAATTGTTATCAGTAAAATACCTGTTATGCAAGCAGGGTTAAAAGAGGTCATGAGGACTCACTTCCCTGAATATGAAATAATATCCAGCGCCTCTg                                             >  1:937487/1‑151 (MQ=255)                                                                                                     tAATTTTTAGACAGTGGATGTGGAGGAAATATGTTGCCAGGATGCTGAAAAAATGGAATTGTTATCAGTAAAATACCTGTTATGCAAGCAGGGTTAAAAGAGGTCATGAGGACTCACTTCCCTGAATATGAAATAATATCCAGCGCCTCTg                                             >  2:594703/1‑151 (MQ=255)                                                                                                      aaTTTTTAGACAGTGGATGTGGAGGAAATATGTTGCCAGGATGCTGAAAAAATGGAATTGTTATCAGTAAAATACCTGTTATGCAAGCAGGGTTAAAAGAGGTCATGAGGACTCACTTCCCTGAATATGAAATAATATCCAGCGCCTCTGc                                            <  1:89448/151‑1 (MQ=255)                                                                                                             aGACAGTGGATGTGGAGGAAATATGTTGCCAGGATGCTGAAAAAATGGAATTGTTATCAGTAAAATACCTGTTATGCAAGCAGGGTTAAAAGAGGTCATGAGGACTCACTTCCCTGAATATGAAATAATATCCAGCGCCTCTGCggagga                                      <  1:994980/150‑1 (MQ=255)                                                                                                             aGACAGTGGATGTGGAGGAAATATGTTGCCAGGATGCTGAAAAAATGGAATTGTTATCAGTAAAATACCTGTTATGCAAGCAGGGTTAAAAGAGGTCATGAGGACTCACTTCCCTGAATATGAAATAATATCCAGCGCCTCTGCGGAGGac                                     <  1:284903/151‑1 (MQ=255)                                                                                                              gACAGTGGATGTGGAGGAAATATGTTGCCAGGATGCTGAAAAAATGGAATTGTTATCAGTAAAATACCTGTTATGCAAGCAGGGTTAAAAGAGGTCATGAGGACTCACTTCCCTGAATATGAAATAATATCCAGCGCCTCTGCGGAGGacc                                    <  1:993567/151‑1 (MQ=255)                                                                                                                cAGTGGATGTGGAGGAAATATGTTGCCAGGATGCTGAAAAAATGGAATTGTTATCAGTAAAATACCTGTTATGCAAGCAGGGTTAAAAGAGGTCATGAGGACTCACTTCCCTGAATATGAAATAATATCCAGCGCCTCTGCGGAGGacctt                                  >  2:1097274/1‑151 (MQ=255)                                                                                                                 aGTGGATGTGGAGGAAATATGTTGCCAGGATGCTGAAAAAATGGAATTGTTATCAGTAAAATACCTGTTATGCAAGCAGGGTTAAAAGAGGTCATGAGGACTCACTTCCCTGAATATGAAATAATATCCAGCGCCTCTGCGGAGGacctt                                  >  1:342196/1‑150 (MQ=255)                                                                                                                      aTGTGGAGGAAATATGTTGCCAGGATGCTGAAAAAATGGAATTGTTATCAGTAAAATACCTGTTATGCAAGCAGGGTTAAAAGAGGTCATGAGGACTCACTTCCCTGAATATGAAATAATATCCAGCGCCTCTGCGGAGGAccttacctta                            <  1:228794/151‑1 (MQ=255)                                                                                                                         tgGAGGAAATATGTTGCCAGGATGCTGAAAAAATGGAATTGTTATCAGTAAAATACCTGTTATGCAAGCAGGGTTAAAAGAGGTCATGAGGACTCACTTCCCTGAATATGAAATAATATCCAGCGCCTCTGCGGAGGACCTTACCttatt                          >  1:896156/1‑150 (MQ=255)                                                                                                                                aaTATGTTGCCAGGATGCTGAAAAAATGGAATTGTTATCAGTAAAATACCTGTTATGCAAGCAGGGTTAAAAGAGGTCATGAGGACTCACTTCCCTGAATATGAAATAATATCCAGCGCCTCTGCGGAGGACCTTACCTTATTACAATTAc                  <  1:883547/151‑1 (MQ=255)                                                                                                                                   atGTTGCCAGGATGCTGAAAAAATGGAATTGTTATCAGTAAAATACCTGTTATGCAAGCAGGGTTAAAAGAGGTCATGAGGACTCACTTCCCTGAATATGAAATAATATCCAGCGCCTCTGCGGAGGACCTTACCTTATTACAATTAcgtc               <  1:263544/151‑1 (MQ=255)                                                                                                                                   atGTTGCCAGGATGCTGAAAAAATGGAATTGTTATCAGTAAAATACCTGTTATGCAAGCAGGGTTAAAAGAGGTCATGAGGACTCACTTCCCTGAATATGAAATAATATCCAGCGCCTCTGCGGAGGACCTTACCTTATTACAATTAcgtc               <  2:487783/151‑1 (MQ=255)                                                                                                                                   atGTTGCCAGGATGCTGAAAAAATGGAATTGTTATCAGTAAAATACCTGTTATGCAAGCAGGGTTAAAAGAGGTCATGAGGACTCACTTCCCTGAATATGAAATAATATCCAGCGCCTCTGCGGAGGACCTTACCTTATTACAATTAcgtc               <  2:611059/151‑1 (MQ=255)                                                                                                                                   atGTTGCCAGGATGCTGAAAAAATGGAATTGTTATCAGTAAAATACCTGTTATGCAAGCAGGGTTAAAAGAGGTCATGAGGACTCACTTCCCTGAATATGAAATAATATCCAGCGCCTCTGCGGAGGACCTTACCTTATTACAATTAcgt                <  1:511048/150‑1 (MQ=255)                                                                                                                                     ggtGCCAGGATGCTGCGAGAGGGGAATTGTTATCAGGACAAGACCTGTTCGGCGGGCAGGGTTACAAGAGTTCTTGATTACTCTCTTCCCTGAATGTGAAAAAATATCCAGCGCCTCTGCGGGGGGCCTTACCTTATTACAATTAcgtcg              <  1:624377/148‑1 (MQ=255)                                                                                                                                     gTTGCCAGGATGCTGAAAAAATGGAATTGTTATCAGTAAAATACCTGTTATGCAAGCAGGGTTAAAAGAGGTCATGAGGACTCACTTCCCTGAATATGAAATAATATCCAGCGCCTCTGCGGAGGACCTTACCTTATTACAATTAcgtcgt             >  1:310352/1‑151 (MQ=255)                                                                                                                                       tGCCAGGATGCTGAAAAAATGGAATTGTTATCAGTAAAATACCTGTTATGCAAGCAGGGTTAAAAGAGGTCATGAGGACTCACTTCCCTGAATATGAAATAATATCCAGCGCCTCTGCGGAGGACCTTACCTTATTACAATTACGTCGtt            <  2:465640/150‑1 (MQ=255)                                                                                                                                       tGCCAGGATGCTGAAAAAATGGAATTGTTATCAGTAAAATACCTGTTATGCAAGCAGGGTTAAAAGAGGTCATGAGGACTCACTTCCCTGAATATGAAATAATATCCAGCGCCTCTGCGGAGGACCTTACCTTATTACAATTACGTCGTTc           >  1:1040205/1‑151 (MQ=255)                                                                                                                                        gCCAGGATGCTGAAAAAATGGAATTGTTATCAGTAAAATACCTGTTATGCAAGCAGGGTTAAAAGAGGTCATGAGGACTCACTTCCCTGAATATGAAATAATATCCAGCGCCTCTGCGGAGGACCTTACCTTATTACAATTACGTCGTTcc          <  1:363289/151‑1 (MQ=255)                                                                                                                                        gCCAGGATGCTGAAAAAATGGAATTGTTATCAGTAAAATACCTGTTATGCAAGCAGGGTTAAAAGAGGTCATGAGGACTCACTTCCCTGAATATGAAATAATATCCAGCGCCTCTGCGGAGGACCTTACCTTATTACAATTACGTCGTTcc          >  2:373008/1‑151 (MQ=255)                                                                                                                                            ggATGCTGAAAAAATGGAATTGTTATCAGTAAAATACCTGTTATGCAAGCAGGGTTAAAAGAGGTCATGAGGACTCACTTCCCTGAATATGAAATAATATCCAGCGCCTCTGCGGAGGACCTTACCTTATTACAATTACGTCGTTCCGGAt      >  2:664541/1‑151 (MQ=255)                                                                                                                                             gATGCTGAAAAAATGGAATTGTTATCAGTAAAATACCTGTTATGCAAGCAGGGTTAAAAGAGGTCATGAGGACTCACTTCCCTGAATATGAAATAATATCCAGCGCCTCTGCGGAGGACCTTACCTTATTACAATTACGTCGTTCCGGAtt     <  2:1009070/151‑1 (MQ=255)                                                                                                                                             gATGCTGAAAAAATGGAATTGTTATCAGTAAAATACCTGTTATGCAAGCAGGGTTAAAAGAGGTCATGAGGACTCACTTCCCTGAATATGAAATAATATCCAGCGCCTCTGCGGAGGACCTTACCTTATTACAATTACGTCGTTCCGGAtt     <  2:12639/151‑1 (MQ=255)                                                                                                                                                gctgaAAAAATGGAATTGTTATCAGTAAAATACCTGTTATGCAAGCAGGGTTAAAAGAGGTCATGAGGACTCACTTCCCTGAATATGAAATAATATCCAGCGCCTCTGCGGAGGACCTTACCTTATTACAATTACGTCGTTCCGGATTAGt  >  2:484581/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                                   AGTATGCTGATATGAAAGTGAATGCTAAGGATAATTTATTCGCTTAATCTATTAATTTGCTGGAAACATTTAAGGAATGAATATCCTGAATAAACCATCATAATTTTTAGACAGTGGATGTGGAGGAAATATGTTGCCAGGATGCTGCAAAAATGGAATTGTTATCAGTAAAATACCTGTTATGCAAGCAGGGTTAAAAGAGGTCATGAGGACTCACTTCCCTGAATATGAAATAATATCCAGCGCCTCTGCGGAGGACCTTACCTTATTACAATTACGTCGTTCCGGATTAGT  >  NC_000913/4603347‑4603640

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑