Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091315,4100TG40.8% 86.8 / 13.6 49intergenic (+112/‑35)dnaJ/insL1chaperone Hsp40, DnaK co‑chaperone/IS186 transposase
Reads supporting (aligned to +/- strand):  ref base T (3/26);  new base G (20/0);  total (23/26)
Fisher's exact test for biased strand distribution p-value = 6.26e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTC  >  NC_000913/15261‑15535
                                                                                                                                                     |                                                                                                                              
aTGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGtt                                                                                                                               <  2:1013/151‑1 (MQ=255)
     gtgAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTgg                                                                                                                           <  2:278128/150‑1 (MQ=255)
       gaagaaGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTa                                                                                                                         <  1:477569/150‑1 (MQ=255)
           aagTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGGCACTCCCCTAAAAGCTAAATTAACGGGTGTggggttttg                                                                                                                    >  2:515378/1‑144 (MQ=255)
                    gacgacCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATg                                                                                                           <  1:483223/151‑1 (MQ=255)
                    gacgacCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATg                                                                                                           <  1:776503/151‑1 (MQ=255)
                     acgacCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGa                                                                                                         <  1:169137/151‑1 (MQ=255)
                       gacCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCCTAAACGCTAACTTAAGGGGTGGGGGATTACGCCTGGTATGAtt                                                                                                       >  1:441679/1‑151 (MQ=255)
                            gACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGTCACTCCCCCAAGCGCGCAATTAAGGGGGTTGGGGTTTCGCCTGCTATTAttt                                                                                                      >  2:439583/1‑147 (MQ=255)
                            gACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACg                                                                                                  <  2:800712/151‑1 (MQ=255)
                                cGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGAGGTGAATCCCCTAAATGAGAACTTAAGCGGTGGGGGAATAAGCCtcacgttactt                                                                                                       >  1:600864/1‑133 (MQ=255)
                                 gCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGTCACTCCCCTAAACGCTCACTTAAAGGGTGTGGTATTACGCCTGATATTATTTAACATGcc                                                                                              >  2:349663/1‑150 (MQ=255)
                                  cTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGACAACCCCACAAACGCTAAACTAAAGGGTGTGGGGTTAAGCCCGGTATTATTTTACGTGGCg                                                                                             >  2:1011487/1‑150 (MQ=255)
                                    aaCCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGTCACTCCCCTAAGCGCCAACTTAAGGGGTGTGGGATTAAGCCTGATATGAttttaaatgcc                                                                                              >  1:687536/1‑139 (MQ=255)
                                    aaCCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGTCACTCCCCTAAACGCTAACTTAAGGGGTGTGGGATTTCGCCTGGTATGATTTTACGTGCCGAt                                                                                           >  1:364427/1‑150 (MQ=255)
                                    aaCCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGAt                                                                                           <  1:683611/150‑1 (MQ=255)
                                    aaCCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATg                                                                                          <  2:234574/151‑1 (MQ=255)
                                         ccccAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGTCACTCCCCTAAACGCTAACTTAAAGGGTGTGGGATTAAGCCCGGTATGATTTTAAATGGCGATGaaat                                                                                      >  1:79019/1‑148 (MQ=255)
                                         ccccAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTa                                                                                     <  1:739831/151‑1 (MQ=255)
                                                aGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGGCACTCCCCTAAGCGCTAAATTAAGGGGTGTGGGATTACGCCTGATATTATTTTAAGTGCCGATGAAATatttt                                                                                 >  2:215669/1‑144 (MQ=255)
                                                aGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGACACCCCCCCAAGCGCTAAATTTAAGGGTGTGGTATTAAGCCCGGTATGATTTTACGTGGCGCAGAAATActtt                                                                                 >  1:805435/1‑146 (MQ=255)
                                                   cTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGTCACTCCCCCAAACGCTAACTTAAAGGGTGTGGGATTAAGCCTGGTATGATTTTAACTGCCGACGAATTAATCTCACGa                                                                            >  1:130465/1‑150 (MQ=255)
                                                    tGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGGGAAACCCAAAAGAGCTAACTTTAGGGGTGTGGGATTAATCCTGATATGATTTTAAATGCCGATGAAtt                                                                                      >  2:633510/1‑139 (MQ=255)
                                                           gggCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGTACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGt                                                                    <  1:630526/150‑1 (MQ=255)
                                                            ggCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCa                                                                  <  2:733897/151‑1 (MQ=255)
                                                              cAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGc                                                                <  1:587611/151‑1 (MQ=255)
                                                                  ccTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAAtt                                                            <  2:432923/151‑1 (MQ=255)
                                                                  ccTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAAtt                                                            <  2:334265/151‑1 (MQ=255)
                                                                     gggTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTg                                                         <  2:524399/151‑1 (MQ=255)
                                                                       gTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGc                                                       <  2:441817/151‑1 (MQ=255)
                                                                          aaaaTAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGGCACTCCCATAAACGCTAACTTTAGGGGTGTGGGATTAAGCCTGATATTATTTAAACTGCCGATGAATTAATCTCACGATAAATGGTCAGCAATTCTGGccc                                                     >  1:893034/1‑150 (MQ=255)
                                                                          aaaaTAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGGCACTCCCACAAACGCCAACTTTAAGGGTGTGGGATTTTGCCTGATATGATTTAAAATGGCCATGAATTACTCTCACCATAAATGGGCAGCAAATCTTGcccc                                                    >  1:924622/1‑150 (MQ=255)
                                                                             aTAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGAAGACTCCCACAAGAGCTTTATTAACGGGTGTGGTTTTTCGCCCGGTAAGTTTTTAATATGCGATGGATTTATCTCTCCATAAATGGGCAGCAAATCTTGccc                                                     >  2:109660/1‑147 (MQ=255)
                                                                               aGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTg                                               <  1:8225/151‑1 (MQ=255)
                                                                                  gTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGGTCACTCCCAAAAGCGCCAAATTAAAGGGTGTGGGAATACGCCTGATATGATTTTAAGTGGCCATGAATTACTCTCACCAAAAATGGGCAGCAAATCTGGGCCATAtttg                                              >  1:385995/1‑147 (MQ=255)
                                                                                     cGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGc                                         <  2:709740/151‑1 (MQ=255)
                                                                                        tGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCAATCCCCTAAACGCTAAATTAAGGGGTGTGGGATTACGCCTGATATGATTTTAAGTGCCCATGGATTACTCTCACGATAACTGGTCAGCAATTTTTGCCCCTATTTGTAAGccc                                       >  1:712871/1‑150 (MQ=17)
                                                                                               atGCGAGCACCTGTAAAGTGGCGGGGGTTACTCCCCTAAACGCTAAATTAAGGGGTGTGGGATTTTGCCCGATATGATTTTACGTGCCGATGAATTTATCTCCCGATAAATGGTCCGCAAATTTGGCCCCTATTTGTAAAACCgaaaaa                                 >  2:932773/1‑149 (MQ=255)
                                                                                               atGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACt                               <  2:655137/151‑1 (MQ=255)
                                                                                                tGCGAGCACCTGTAAAGTGGCGGGGGGAACCCCCCTAAACGCTAACTTAAGGGGTGTGGGGTTACGCCTGATATGATTTAAAATGGCGATGAATTAATCTCACGATAAATGGTCAGCAATTTTGGCCCCTATTTGTAAACCCgaagaa                                 >  1:184186/1‑148 (MQ=255)
                                                                                                     gCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATAc                         <  1:936605/151‑1 (MQ=255)
                                                                                                                 tGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTa              >  2:169453/1‑150 (MQ=35)
                                                                                                                    cGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGc          <  2:336652/151‑1 (MQ=33)
                                                                                                                      gggATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCg        <  1:425287/151‑1 (MQ=33)
                                                                                                                        gATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCggg      >  1:173801/1‑151 (MQ=33)
                                                                                                                        gATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCggg      <  2:864613/151‑1 (MQ=33)
                                                                                                                         aTCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCgggg     <  1:602670/151‑1 (MQ=33)
                                                                                                                           cACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGct   <  1:914268/151‑1 (MQ=31)
                                                                                                                            aCTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGctc  <  1:604597/151‑1 (MQ=31)
                                                                                                                            aCTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGctc  >  2:319837/1‑151 (MQ=31)
                                                                                                                                                     |                                                                                                                              
ATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCCGTGGGCAGGCCTGGGTAAAAATAGGGTGCGTTGAAGATATGCGAGCACCTGTAAAGTGGCGGGGATCACTCCCATAAGCGCTAACTTAAGGGTTGTGGTATTACGCCTGATATGATTTAACGTGCCGATGAATTACTCTCACGATAACTGGTCAGCAATTCTGGCCCATATTGGTAAGCCCGAAGAACTGGATACTTCGGCACGTAATGCCGGGGCTC  >  NC_000913/15261‑15535

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: