Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,624,5170GT49.1% 63.4 / 36.1 59E492* (GAA→TAA) ppkpolyphosphate kinase, component of RNA degradosome
Reads supporting (aligned to +/- strand):  ref base G (8/22);  new base T (29/0);  total (37/22)
Fisher's exact test for biased strand distribution p-value = 8.27e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTAAAGAAAACGGTGAAGTGGTGCGTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTATTTAACTTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTATCGCCGCAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATAACCTTGTC  >  NC_000913/2624371‑2624666
                                                                                                                                                   |                                                                                                                                                       
gTAAAGAAAACGGTGAAGTGGTGCGTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTAATTAAATTTTTTTaaa                                                                                                                                                      >  2:342925/1‑150 (MQ=255)
  aaaGAAAACGGTGAAGTGGTGCGTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGGATTTTAATTTATTTaaaaa                                                                                                                                                    >  1:57625/1‑149 (MQ=255)
   aaGAAAACGGTGAAGTGGTGCGTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTATTTTAATTTTTTTaaaaaac                                                                                                                                                  >  2:116376/1‑148 (MQ=255)
    aGAAAACGGTGAAGTGGTGCGTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTATTTAAATTTATTTAAAAacc                                                                                                                                                  >  1:968007/1‑150 (MQ=255)
            gTGAAGTGGTGCGTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTATTTAACTTTATTGAAAACCCATACCGTcc                                                                                                                                        <  1:762417/151‑1 (MQ=255)
               aaGTGGTGCGTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGGATTTTACTTTTTTTTAAAACCATACCGTCCggg                                                                                                                                     >  1:971745/1‑150 (MQ=255)
                 gtggtgCGTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGGTTTTTAATTTTTTTAAAACCCCAACCCCCCGGTg                                                                                                                                    >  2:865549/1‑150 (MQ=255)
                  aggtgCGTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGGAGTTTAATTTATTTAAAAacccaaccaccc                                                                                                                                         >  2:86397/2‑136 (MQ=255)
                    gtgCGATATGCACACATCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGTCTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGAGCTTTTAGGTTTTTTAAAAACCATCCCGTcctggggc                                                                                                                                  >  1:806627/1‑143 (MQ=255)
                     tgCGTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGGATTTAACTTTTTTTAAAAACCCTACCGCCCGGGGGCAtt                                                                                                                               >  2:750307/1‑151 (MQ=255)
                           aTGCACACATCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTATTTTACTTTATTGAAAAACCATACCCTCCGGGGGCATTTGatt                                                                                                                          >  1:63645/1‑150 (MQ=255)
                                 acaTCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGGATTTTACTTTTTTTAAAAACCACACCGTCCGGGGGCATTTTTTTAttt                                                                                                                      >  2:132887/1‑148 (MQ=255)
                                     cGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTATTTAACTTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTa                                                                                                               <  1:1032119/151‑1 (MQ=255)
                                     cGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTATTTAACTTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTa                                                                                                               <  2:826671/151‑1 (MQ=255)
                                       ggACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTATTTTACTTTTTTTAAAAACCACACCCTCCCGGGGCAttttttttttttattg                                                                                                                 >  2:711390/1‑134 (MQ=255)
                                        gACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTATTTTACTTTTTTTAAAAACCAAACCCTCCGGGGAGATTTTATTTTTTTATTGTATcg                                                                                                            >  1:813525/1‑151 (MQ=255)
                                             ggAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTATTTAACTTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTATCGCCGCa                                                                                                       <  1:937619/151‑1 (MQ=255)
                                                aCTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGGATTTAAATTTTTTTAAAACCCCAACCCTCCGGGGACAATTGTTTATTTTATTGTATtgccgcca                                                                                                      >  1:869547/1‑147 (MQ=255)
                                                 cTTTAACGACAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGCCATTTATCTTTTTTTTAAACCCCAACCCTCCGGGGGGCTTTGATTTTTTTCTTGTATCTGCGCgaaa                                                                                                    >  2:256218/1‑150 (MQ=255)
                                                   ttAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGGATTTAACTTTTTTTAAAACCCCTACCCCCCGGGGGCAATTTATTTTTTTATTGTATTGCCCCaaaa                                                                                                    >  2:150276/1‑148 (MQ=255)
                                                    tAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTATTTAACTTTTTTTAAAAACCACAACCTCCGGTGGCATTTTATTTTTTTTTGGTATCGCCCCcaaaaacc                                                                                                >  1:585752/1‑147 (MQ=255)
                                                     aaCGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTATTTAACTTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTATCGCCGCAAAACTccc                                                                                               <  1:673027/151‑1 (MQ=255)
                                                     aaCGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTATTTAACTTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTATCGCCGCAAAACTcc                                                                                                <  1:468178/150‑1 (MQ=255)
                                                       cGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGGCTTTAACTTTATTTTCAACCCACACCCTCCCGGGAAATTTTTTTTTTTTATCGTATTGCGGCAAAACTccccc                                                                                             >  2:176942/1‑149 (MQ=255)
                                                        gAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTATTTAACTTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTATCGCCGCAAAACTCccgc                                                                                             <  2:590361/150‑1 (MQ=255)
                                                        gAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGGGTTTTACTTTTTTTTAAAACCCAACCGTCCCGGGGCATTTTTTTTTTTTATTGTATTGCCGCcaaaaacccccc                                                                                            >  2:551421/1‑143 (MQ=255)
                                                          aaaaaCCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGGATTTTAATTTTTTTAAAAACCATACCGTCCCGGGGCATTTGTTTATTTTATGGTATCGCCGCCAAAATCCcgccgc                                                                                          >  1:307418/1‑151 (MQ=255)
                                                          aaaaaCCGCGCGTCTTTAGACTGGCTATTCGTTGTTGACCGCCGTTGCGCGCATCACCAACGAAGTACGGCGGGTATTTAACTTTATTGAAGACCCGTACCGGCCGGTGACTTTTGATTATTTAATGGTATCGCCGCAAAACTCccgc                                                                                             <  2:650386/148‑1 (MQ=255)
                                                                 gcgcgTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTATTTTACTTTTTTTAAAAACCCTACCCCCCGGCGACATTTGATTATTTTATTGTATTGCCCCCAAACTCCCCCCCCCCAtttt                                                                                   >  2:922544/1‑149 (MQ=255)
                                                                    cgTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTATTTAACTTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTATCGCCGCAAAACTCCCGCCGCCTATTGTAt                                                                                 <  2:895740/150‑1 (MQ=255)
                                                                     gTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTGTTTTACTTTTTTTAAAAACCATACCATCCGGTGAAATTTTTTTTTTTAATGGAATTGCCGCAAAACACCCCCCGCCCCTTGt                                                                                   >  1:876621/1‑147 (MQ=255)
                                                                         ttATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTATTTAACTTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTATCGCCGCAAAACTCCCGCCGCCTATTGTATGaa                                                                              <  1:115574/148‑1 (MQ=255)
                                                                         ttATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGGATTTAACTTTTTTTAAAACCCATACCCGCCCGGGGCATTTTGTTTTTTTATTGGATTGCCCCAAAAAACCCCCCGCCCATTTTATGaaa                                                                             >  1:550494/1‑149 (MQ=255)
                                                                            taCTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTATTTTACTTTATTTAAAACCCCAACCCGCCCGGGACATTTTATTTTTTTATGGGAATGCCGCAAAACTCCCgccgcccgttttttgtaa                                                                             >  1:407029/1‑134 (MQ=255)
                                                                            taCTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTATTTTAATTTATTTAAAACCCCAAACGCCACGGTGCATTTTGTTTTTTTATGGGTTACCCCCAAAAACCCCGCCCCCCATTGTATGAAAtggtg                                                                        >  2:1065585/1‑151 (MQ=255)
                                                                             actaactaTTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTATTTAACTTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTATCGCCGCAAAACTCCCGCCGCCTATTGTATGAAAtggtg                                                                        <  2:691510/150‑1 (MQ=255)
                                                                               tGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGGATTTAACTTTATTGAAAAACCATACCCTCCGGGGGCATTTGATTTTTTTATGGGATCGCCGCAAAACTCCCCCCGCCTATTGTATGAAAtggtgg                                                                       >  1:741491/1‑149 (MQ=255)
                                                                                 aCTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTATTTTACTTTATTTTAAACCCATACCCTCCGGTGGCATTTGGTTTTTTTATTGTATCGCCGCAAAAAACCCCCCGCCCATTGTATGAAAtggtgg                                                                       >  2:437135/1‑147 (MQ=255)
                                                                                 aCTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTATTTAACTTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTATCGCCGCAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACcg                                                                   <  1:96591/151‑1 (MQ=255)
                                                                                  cTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTATTTAACTTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTATCGCCGCAAAACTCCCGCCGCCTATTGTATGAAATGGTGGAcc                                                                    <  2:954634/149‑1 (MQ=255)
                                                                                     ttCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTATTTTACTTTTTTGTAAAACCCTACCGTCCCGGGACATTTGATTATTTTATTGTATTGGCGCCAAAATACCCCCGCCCACTGTATGaaaatgggggccg                                                                   >  2:382009/1‑137 (MQ=255)
                                                                                       cGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTATTTAACTTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTATCGCCGCAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCgaga                                                             <  1:630736/150‑1 (MQ=255)
                                                                                       cGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTATTTAACTTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTATCGCCGCAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGAt                                                            <  1:29361/151‑1 (MQ=255)
                                                                                                 cgccgATGCGCGCATCACCAACGAAGTACGGCGGGTGTTTTACATTTTTTTTAAACCCATTCGCCCGGGGGAAATTTTTTATTTTATTGTATTTACCCAAAACTCCCGCCGCCCCCTGTATTGAATTGCGGAACGCGGGAATCGCCacac                                                    >  2:862013/1‑147 (MQ=255)
                                                                                                    cgATGCGCGCATCACCAACGAAGTACGGCGGGTATTTAACTTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATAGTATCGCCGCAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCgcag                                               <  2:865547/151‑1 (MQ=255)
                                                                                                    cgATGCGCGCATCACCAACGAAGTACGGCGGGGATTTTACTTTTTTTTAAAACCCTACCCTCCGGGGGCATTTGATTTTTTTATTGTATTGCCCCAAAAATCCCCCCCCCCCTTTTATTAAAAAGTGGACCGCGGGAGCGCCCAccc                                                   >  2:583049/1‑145 (MQ=255)
                                                                                                           cgcATCACCAACGAAGTACGGCGGGTATTTTACTTTATTTAAAACCCCTACCCTCCGGGGGCATTTTATTATTTTATGGGATCGCCGCAAAAATCCCGCCCCCCCTTTTTTGAAAATGGGGGCCGCGAGATCGCCAACCCGCAGCAAagg                                         >  1:333263/1‑150 (MQ=255)
                                                                                                                 aCCAACGAAGTACGGCGGGTATTTAACTTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTATCGCCGCAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCa                                  <  1:579747/151‑1 (MQ=255)
                                                                                                                 aCCAACGAAGTACGGCGGGTATTTAACTTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTATCGCCGCAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCa                                  <  1:797712/151‑1 (MQ=255)
                                                                                                                  ccAACGAAGTACGGCGGGTATTTTACTTTATTTAAAACCCAAACCCCCCGGGGGCAATTTTTTTTTTTATTGTATTGCCCCAAAAATACCCCCGCCTATTTTATGAAAAGGTGGGCCGCGGGAACGGCCACCCCCAGCCAAGGccggcc                                   >  2:1048211/1‑144 (MQ=255)
                                                                                                                  ccAACGAAGTACGGCGGGTATTTAACTTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTATCGCCGCAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAg                                 <  1:190472/151‑1 (MQ=255)
                                                                                                                   cAACGAAGTACGGCGGGTATTTAACTTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTATCGCCGCAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGt                                <  1:296449/151‑1 (MQ=255)
                                                                                                                             cggcggGTATTTAACTTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTATCGCCGCAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCAccc                      <  2:813401/151‑1 (MQ=255)
                                                                                                                                cggGGATTTAACTTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTATCGCCGCAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTg                    >  1:711269/1‑150 (MQ=255)
                                                                                                                                 gggTATTTAACTTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTATCGCCGCAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGaa                  <  1:757480/151‑1 (MQ=255)
                                                                                                                                          aCTTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTATCGCCGCAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATa          >  2:298118/1‑150 (MQ=255)
                                                                                                                                                 ttGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTATCGCCGCAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATAACCTTGt   >  2:331468/1‑151 (MQ=255)
                                                                                                                                                  tGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTATCGCCGCAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATAACCTTGTc  >  2:443569/1‑151 (MQ=255)
                                                                                                                                                  tGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTATCGCCGCAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATAACCTTGTc  >  1:530136/1‑151 (MQ=255)
                                                                                                                                                   |                                                                                                                                                       
GTAAAGAAAACGGTGAAGTGGTGCGTTATGCACACATCGGGACCGGGAACTTTAACGAAAAAACCGCGCGTCTTTATACTGACTATTCGTTGCTGACCGCCGATGCGCGCATCACCAACGAAGTACGGCGGGTATTTAACTTTATTGAAAACCCATACCGTCCGGTGACATTTGATTATTTAATGGTATCGCCGCAAAACTCCCGCCGCCTATTGTATGAAATGGTGGACCGCGAGATCGCCAACGCGCAGCAAGGGCTGCCCAGTGGTATCACCCTGAAGCTAAATAACCTTGTC  >  NC_000913/2624371‑2624666

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: