Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,423,2870GC44.0% 73.2 / 26.2 51intergenic (+93/+136)yhdZ/rrfFputative amino acid ABC transporter ATPase/5S ribosomal RNA of rrnD operon
Reads supporting (aligned to +/- strand):  ref base G (4/24);  new base C (22/0);  total (26/25)
Fisher's exact test for biased strand distribution p-value = 1.72e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTGCGCATCCTAAATCAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGT  >  NC_000913/3423138‑3423434
                                                                                                                                                      |                                                                                                                                                        
tttGCGCATCCTAAATCAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCg                                                                                                                                                          <  2:474580/150‑1 (MQ=255)
    cgcATCCTAAATCAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCAcc                                                                                                                                                     <  2:285834/151‑1 (MQ=255)
      cATCCTAAATCAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCCGccccccccccc                                                                                                                                                   >  1:994828/1‑150 (MQ=255)
        tCCTAAATCAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTc                                                                                                                                                  <  2:927640/150‑1 (MQ=255)
         ccTAAATCAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTcc                                                                                                                                                 <  1:962085/150‑1 (MQ=255)
                cAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGGCCTGCcccccccccccc                                                                                                                                                 >  1:360904/1‑143 (MQ=255)
                 agagCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGGGTCCCTCCCCCCCCCCCCCCGGCCg                                                                                                                                           >  1:1021714/1‑148 (MQ=255)
                             ggCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGtt                                                                                                                          <  2:638468/151‑1 (MQ=255)
                               cATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCCCCCCCCCGGCCGTTGCTTCGCAACGtt                                                                                                                          >  1:423807/1‑149 (MQ=255)
                               cATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCa                                                                                                                        <  2:264754/151‑1 (MQ=255)
                                 tttttATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGGCCCGCCCCCCCCCCCCCCGGCCGGTTTTTTGCAACggttca                                                                                                                        >  2:910165/1‑145 (MQ=25)
                                  ttttATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCaaa                                                                                                                      <  2:390846/150‑1 (MQ=255)
                                           aGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCCCCCGGCCGTTGCTTCGCAACGTTCAAATcccccccc                                                                                                             >  1:580358/1‑150 (MQ=255)
                                               aaTCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCCCCCCCCCCCCGGCCGTTGCTTCGCCACGTTCaaaaaccccccccgcgg                                                                                                        >  1:861588/1‑137 (MQ=21)
                                                aTCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCCCCCCCCGGGCCGGTGCTTCGCAACGTTCAAATCCGCCCCcgccgg                                                                                                       >  2:370558/1‑150 (MQ=255)
                                                  cccttACTTGAATGTTAGTTCGAAAAGCAAAAAGTCCCTCCTTTCTTCTTTCCTTTCTCTTTCTTTTCTTTCTTTCCTTTTCTTTCTTTCTTCCTGCCCCCCCCCCCCCCGGCCGTTGCTTTGCAACGTTTAAATACCCTcccctcgggtt                                                                                                     >  2:956079/4‑143 (MQ=255)
                                                   cATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTGCTGCCCCCCCCCCCCCGGGGCGTTGCTTCGCAAAGTTCAAATACGCCCCCGGCGGGttt                                                                                                   >  2:496036/1‑151 (MQ=255)
                                                         ttGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGGCCTGCCCCCCCCCCCCCCGGCCGTTGCTTCGCAACGTTTAAATCCGCCCCCCGCGGGTTTGTcc                                                                                               >  1:399539/1‑149 (MQ=18)
                                                           gAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCCGCCCCCCCCCCTCCGGGCCGCTGCTTCGCCACGCTCTAATCCGCTCCCGGCGGGTTTTTCCCAc                                                                                            >  2:41850/1‑150 (MQ=18)
                                                            aaTGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGACCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTc                                                                                         <  2:186893/151‑1 (MQ=255)
                                                               gTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCCGCCCCCCCCCCCCCCGGCCGGTTCTTCTCAACCTTCAAAACCGCCCCCCGCGGGGTTTTCCtccatgcg                                                                                       >  2:303780/1‑144 (MQ=17)
                                                               gTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGGCCCGCCCCCCACCCTCCCGGGCCTTGCTTTGCAAAGTTTAAATTCCCTCCCCGCGGGTTTGTCCTCCTCgg                                                                                       >  2:63570/1‑150 (MQ=255)
                                                                   gTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGagag                                                                                  <  1:829216/151‑1 (MQ=255)
                                                                     tCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAgt                                                                                 <  1:199075/150‑1 (MQ=255)
                                                                       gAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGGCCTGCCCCCCCCCCCCCCGGCCGTTGCTTCCCAACGGTCAAATCCGCCCCCCGCGGGTTTGTTCTACCCgggggggggt                                                                               >  2:926192/1‑143 (MQ=255)
                                                                         aaaGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCa                                                                            <  2:99139/151‑1 (MQ=255)
                                                                          aaGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCAc                                                                           <  1:716141/151‑1 (MQ=255)
                                                                              aaaaaGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGac                                                                       <  1:1039781/151‑1 (MQ=255)
                                                                              aaaaaGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGGCCCGCCCCCCCCCCCCCCGGCCGTTGCTTCGCAACGTTCAAATCCGCCCCCGGCGGGTTTGTCCTACCCCGGAGAGGGGTCAcc                                                                          >  1:472168/1‑148 (MQ=14)
                                                                                aaaGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGacaa                                                                     <  1:1013910/151‑1 (MQ=255)
                                                                                 aaGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTTGCCCCCCCCCCTCGGGCCGGGGGTTTGCAAGGCTTAAAATCGGCCCCCGCGGGTTTGTTTTACTCCGGAGAGGGtttccttaaaa                                                                     >  1:205924/1‑141 (MQ=9)
                                                                                   ggCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGacaaaca                                                                  <  1:490165/151‑1 (MQ=255)
                                                                                     ccATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCCCCCCCCCCCCGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCCGCGGGTTTGTTCTCCTCCGGAGAGGGTTCACCCccaaa                                                                    >  1:488248/1‑147 (MQ=14)
                                                                                      cATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTTCCCCCCACCCCCCCGGGCGTTGCTTCGCAACGTTTAAAATCCCTCCCCGCGGGGTTGTTCTACTCCGGGGGGTGTTTTCCCacaaaaaa                                                                 >  1:121874/1‑149 (MQ=12)
                                                                                       aTCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAg                                                              <  1:785111/151‑1 (MQ=255)
                                                                                         ccTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGAt                                                           <  2:595999/151‑1 (MQ=255)
                                                                                          cTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCCCCACCCCCCCGGCCGCTGCTTCGCAAAGTTCAAATCCGCCCCCCGCGGGTTTGTCCTACTCCGGGGGGGGGTTCCCCACAAacaaca                                                               >  2:811637/1‑147 (MQ=14)
                                                                                            ttCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATaa                                                         <  2:986242/150‑1 (MQ=255)
                                                                                            ttCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCCCCCCCCCCCCGGCCGGTGCTTTGCAAACGTTTAATCCGCTCCCCGCGGGGTTTTTCCTCTCCGGGGGGGGGTTCCCCACAAAAAAAAGATaa                                                         >  2:312419/1‑150 (MQ=9)
                                                                                            ttCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCCCCACCCCCCCGGCCGTTGCTTCGCAACGTTCAAATCCGCCCCCCGCGGGTTTTTCCTACTCCGGAGGGTGTTCACCCACAAACAACAGATaa                                                         >  1:281814/1‑150 (MQ=14)
                                                                                             tCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTGCCGCCCCCCCCCCTCCCGGCCGCTTCTTCCCAACGCTCAAATCCGCCCCCCGCGGGTTTGTTCCCCTCCGGGGAGTGTTTACCGACCAAAAACAAATaaa                                                        >  1:590351/1‑150 (MQ=9)
                                                                                               ggATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACa                                                     <  2:432538/151‑1 (MQ=255)
                                                                                                  tGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACaaa                                                   <  1:757975/150‑1 (MQ=255)
                                                                                                  tGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCCCCCCCCCCCCGGCCGTTGCTTCCCCACGTTCAAAACCGCCCCCCGCGGGTTTTTCCTACTCCGGAGAGTGGTTACCCCCAAACAACAGATaaaaaaaa                                                   >  1:961652/1‑150 (MQ=14)
                                                                                                      cTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCCCCCCCCTCCCGGGCGGTGGTTCGCAAAGTTCAAATTCTCTCCCCGCGGATGTGTTCTACTCGGGGGAGGGTTCACCGCCAAAAAACAGATAaaaaaaaaa                                                 >  2:251331/1‑147 (MQ=14)
                                                                                                          cGCTTGATTTGATGTCTGGCATTTTATGGCGGGCGTCCTGCCCTCAACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAg                                           <  1:167479/150‑1 (MQ=255)
                                                                                                           gCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCCCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCCCTCCCCGCGGGTTTGTCCTACTCCGGAGAGGGTTCACCCACAAAAAACAGATAAAAAAAAAAGCCCACTc                                         >  1:867989/1‑151 (MQ=11)
                                                                                                                 tttgatGCCTGGCAGTTTATGGCGGGCGTCCCGCCCCCCACCCCCCCGGCCGGTGCTTCGCAACGTTCAAATCCGCCCCCCGCGGGTTTTTCCTCCTCAAGAGAGCGGTCACCCACAAAAAACAGAAAAAAACaaaaacccccgcttcc                                      >  1:774691/1‑137 (MQ=0)
                                                                                                                   tgatGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGa                                   <  1:291025/149‑1 (MQ=38)
                                                                                                                   tgatGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGAc                                  <  2:931279/150‑1 (MQ=38)
                                                                                                                                 ttATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGttt                    <  2:756714/150‑1 (MQ=38)
                                                                                                                                      gcgggcgTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTg              <  2:259070/151‑1 (MQ=38)
                                                                                                                                          gcgTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGt          <  1:836551/151‑1 (MQ=39)
                                                                                                                                                   cccGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGt  >  2:307990/1‑151 (MQ=40)
                                                                                                                                                      |                                                                                                                                                        
TTTGCGCATCCTAAATCAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTGCCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGT  >  NC_000913/3423138‑3423434

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: