Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,423,287 | 0 | G | C | 44.0% | 73.2 / 26.2 | 51 | intergenic (+93/+136) | yhdZ/rrfF | putative amino acid ABC transporter ATPase/5S ribosomal RNA of rrnD operon |
Reads supporting (aligned to +/- strand): ref base G (4/24); new base C (22/0); total (26/25) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.72e-10 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TTTGCGCATCCTAAATCAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCC‑TGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACG‑TTCAAATCCGCTCCCGGC‑GGATTTGTCCTACT‑CGGGAGAGTGTTCACCGACAAACAACAG‑ATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGT > NC_000913/3423138‑3423434 | tttGCGCATCCTAAATCAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCC‑TGCCCg < 2:474580/150‑1 (MQ=255) cgcATCCTAAATCAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCC‑TGCCCGCCAcc < 2:285834/151‑1 (MQ=255) cATCCTAAATCAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCC‑CGccccccccccc > 1:994828/1‑150 (MQ=255) tCCTAAATCAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCC‑TGCCCGCCACCCTc < 2:927640/150‑1 (MQ=255) ccTAAATCAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCC‑TGCCCGCCACCCTcc < 1:962085/150‑1 (MQ=255) cAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGGCC‑TGCcccccccccccc > 1:360904/1‑143 (MQ=255) agagCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGGGTCC‑CTCCCCCCCCCCCCCCGGCCg > 1:1021714/1‑148 (MQ=255) ggCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCC‑TGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACG‑tt < 2:638468/151‑1 (MQ=255) cATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCC‑TGCCCGCCCCCCCCCCGGCCGTTGCTTCGC‑AACG‑tt > 1:423807/1‑149 (MQ=255) cATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCC‑TGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACG‑TTCa < 2:264754/151‑1 (MQ=255) tttttATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGGCC‑CGCCCCCCCCCCCCCCGGCCGGTTTTTTGC‑AACggttca > 2:910165/1‑145 (MQ=25) ttttATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCC‑TGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACG‑TTCaaa < 2:390846/150‑1 (MQ=255) aGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCC‑TGCCCGCCACCCCCCCGGCCGTTGCTTCGC‑AACG‑TTCAAATcccccccc > 1:580358/1‑150 (MQ=255) aaTCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCC‑TGCCCCCCCCCCCCCCGGCCGTTGCTTCGC‑CACG‑TTCaaaaaccccccccgcgg > 1:861588/1‑137 (MQ=21) aTCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCC‑TGCCCGCCCCCCCCCGGGCCGGTGCTTCGC‑AACG‑TTCAAATCCGCCCCcgcc‑gg > 2:370558/1‑150 (MQ=255) cccttACTTGAATGTTAGTTCGAAAAGCAAAAAGTCCCTCCTTTCTTCTTTCCTTTCTCTTTCTTTTCTTTCTTTCCTTTTCTTTCTTTCTTCC‑TGCCCCCCCCCCCCCCGGCCGTTGCTTTGC‑AACG‑TTTAAATACCCTcccctcgggtt > 2:956079/4‑143 (MQ=255) cATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTGC‑TGCCCCCCCCCCCCCGGGGCGTTGCTTCGC‑AAAG‑TTCAAATACGCCCCCGGC‑GGGttt > 2:496036/1‑151 (MQ=255) ttGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGGCC‑TGCCCCCCCCCCCCCCGGCCGTTGCTTCGC‑AACG‑TTTAAATCCGCCCCCCGC‑GGGTTTGTcc > 1:399539/1‑149 (MQ=18) gAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCC‑CGCCCCCCCCCCTCCGGGCCGCTGCTTCGC‑CACG‑CTCTAATCCGCTCCCGGC‑GGGTTTTTCCCAc > 2:41850/1‑150 (MQ=18) aaTGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGACCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCC‑TGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACG‑TTCAAATCCGCTCCCGGC‑GGATTTGTCCTACT‑c < 2:186893/151‑1 (MQ=255) gTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCC‑CGCCCCCCCCCCCCCCGGCCGGTTCTTCTC‑AACC‑TTCAAAACCGCCCCCCGC‑GGGGTTTTCCtccatgcg > 2:303780/1‑144 (MQ=17) gTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGGCC‑CGCCCCCCACCCTCCCGGGCCTTGCTTTGC‑AAAG‑TTTAAATTCCCTCCCCGC‑GGGTTTGTCCTCCT‑Cgg > 2:63570/1‑150 (MQ=255) gTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCC‑TGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACG‑TTCAAATCCGCTCCCGGC‑GGATTTGTCCTACT‑CGGGagag < 1:829216/151‑1 (MQ=255) tCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCC‑TGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACG‑TTCAAATCCGCTCCCGGC‑GGATTTGTCCTACT‑CGGGAGAgt < 1:199075/150‑1 (MQ=255) gAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGGCC‑TGCCCCCCCCCCCCCCGGCCGTTGCTTCCC‑AACG‑GTCAAATCCGCCCCCCGC‑GGGTTTGTTCTACC‑Cgggggggggt > 2:926192/1‑143 (MQ=255) aaaGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCC‑TGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACG‑TTCAAATCCGCTCCCGGC‑GGATTTGTCCTACT‑CGGGAGAGTGTTCa < 2:99139/151‑1 (MQ=255) aaGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCC‑TGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACG‑TTCAAATCCGCTCCCGGC‑GGATTTGTCCTACT‑CGGGAGAGTGTTCAc < 1:716141/151‑1 (MQ=255) aaaaaGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCC‑TGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACG‑TTCAAATCCGCTCCCGGC‑GGATTTGTCCTACT‑CGGGAGAGTGTTCACCGac < 1:1039781/151‑1 (MQ=255) aaaaaGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGGCC‑CGCCCCCCCCCCCCCCGGCCGTTGCTTCGC‑AACG‑TTCAAATCCGCCCCCGGC‑GGGTTTGTCCTACC‑CCGGAGAGGGGTCAcc > 1:472168/1‑148 (MQ=14) aaaGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCC‑TGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACG‑TTCAAATCCGCTCCCGGC‑GGATTTGTCCTACT‑CGGGAGAGTGTTCACCGacaa < 1:1013910/151‑1 (MQ=255) aaGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCCTTGCCCCCCCCCCT‑CGGGCCGGGGGTTTGC‑AAGGCTTAAAATCGGC‑CCCCGC‑GGGTTTGTTTTACT‑CCGGAGAGGGtttccttaaaa > 1:205924/1‑141 (MQ=9) ggCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCC‑TGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACG‑TTCAAATCCGCTCCCGGC‑GGATTTGTCCTACT‑CGGGAGAGTGTTCACCGacaaaca < 1:490165/151‑1 (MQ=255) ccATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCC‑TGCCCCCCCCCCCCCCGGCCGTTGCTTCGC‑AACG‑TTCAAATCCGCTCCCCGC‑GGGTTTGTTCTCCT‑CCGGAGAGGGTTCACCCccaaa > 1:488248/1‑147 (MQ=14) cATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCC‑TTCCCCCCACCCCCCCGGGCGTTGCTTCGC‑AACG‑TTTAAAATCCCTCCCCGC‑GGGGTTGTTCTACT‑CCGGGGGGTGTTTTCCCacaaaaaa > 1:121874/1‑149 (MQ=12) aTCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCC‑TGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACG‑TTCAAATCCGCTCCCGGC‑GGATTTGTCCTACT‑CGGGAGAGTGTTCACCGACAAACAACAg < 1:785111/151‑1 (MQ=255) ccTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCC‑TGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACG‑TTCAAATCCGCTCCCGGC‑GGATTTGTCCTACT‑CGGGAGAGTGTTCACCGACAAACAACAG‑At < 2:595999/151‑1 (MQ=255) cTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCC‑TGCCCCCCACCCCCCCGGCCGCTGCTTCGC‑AAAG‑TTCAAATCCGCCCCCCGC‑GGGTTTGTCCTACTCCGGGGGGGGGTTC‑CCCACAAacaaca > 2:811637/1‑147 (MQ=14) ttCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCC‑TGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACG‑TTCAAATCCGCTCCCGGC‑GGATTTGTCCTACT‑CGGGAGAGTGTTCACCGACAAACAACAG‑ATaa < 2:986242/150‑1 (MQ=255) ttCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCC‑TGCCCCCCCCCCCCCCGGCCGGTGCTTTGCAAACG‑TT‑TAATCCGCTCCCCGCGGGGTTTTTCCTCTC‑CGGGGGGGGGTTC‑CCCACAAAAAAAAG‑ATaa > 2:312419/1‑150 (MQ=9) ttCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCC‑TGCCCCCCACCCCCCCGGCCGTTGCTTCGC‑AACG‑TTCAAATCCGCCCCCCGC‑GGGTTTTTCCTACT‑CCGGAGGGTGTTCACCCACAAACAACAG‑ATaa > 1:281814/1‑150 (MQ=14) tCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTGC‑CGCCCCCCCCCCTCCCGGCCGCTTCTTCCC‑AACG‑CTCAAATCCGCCCCCCGC‑GGGTTTGTTCCCCT‑CCGGGGAGTGTTTACCGACCAAAAACAA‑ATaaa > 1:590351/1‑150 (MQ=9) ggATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCC‑TGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACG‑TTCAAATCCGCTCCCGGC‑GGATTTGTCCTACT‑CGGGAGAGTGTTCACCGACAAACAACAG‑ATAAAACa < 2:432538/151‑1 (MQ=255) tGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCC‑TGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACG‑TTCAAATCCGCTCCCGGC‑GGATTTGTCCTACT‑CGGGAGAGTGTTCACCGACAAACAACAG‑ATAAAACaaa < 1:757975/150‑1 (MQ=255) tGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCC‑TGCCCCCCCCCCCCCCGGCCGTTGCTTCCC‑CACG‑TTCAAAACCGCCCCCCGC‑GGGTTTTTCCTACT‑CCGGAGAGTGGTTACCCCCAAACAACAG‑ATaaaaaaaa > 1:961652/1‑150 (MQ=14) cTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCC‑TGCCCCCCCCCCTCCCGGGCGGTGGTTCGC‑AAAG‑TTCAAATTCTCTCCCCGC‑GGATGTGTTCTACT‑CGGGGGAGGGTTCACCGCCAAAAAACAG‑ATAaaaaaaaaa > 2:251331/1‑147 (MQ=14) cGCTTGATTTGATGTCTGGCATTTTATGGCGGGCGTCC‑TGCCCTCAACCCTCCGGGCCGTTGCTTCGC‑AACG‑TTCAAATCCGCTCCCGGC‑GGATTTGTCCTACT‑CGGGAGAGTGTTCACCGACAAACAACAG‑ATAAAACAAAAGGCCCAg < 1:167479/150‑1 (MQ=255) gCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCC‑TGCCCCCCACCCTCCGGGCCGTTGCTTCGC‑AACG‑TTCAAATCCCCTCCCCGC‑GGGTTTGTCCTACT‑CCGGAGAGGGTTCACCCACAAAAAACAG‑ATAAAAAAAAAAGCCCACTc > 1:867989/1‑151 (MQ=11) tttgatGCCTGGCAGTTTATGGCGGGCGTCC‑CGCCCCCCACCCCCCCGGCCGGTGCTTCGC‑AACG‑TTCAAATCCGCCCCCCGC‑GGGTTTTTCCTCCT‑CAAGAGAGCGGTCACCCACAAAAAACAGAAAAAAACaaaaacccccgcttcc > 1:774691/1‑137 (MQ=0) tgatGTCTGGCAGTTTATGGCGGGCGTCC‑TGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACG‑TTCAAATCCGCTCCCGGC‑GGATTTGTCCTACT‑CGGGAGAGTGTTCACCGACAAACAACAG‑ATAAAACAAAAGGCCCAGTCTTCCGa < 1:291025/149‑1 (MQ=38) tgatGTCTGGCAGTTTATGGCGGGCGTCC‑TGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACG‑TTCAAATCCGCTCCCGGC‑GGATTTGTCCTACT‑CGGGAGAGTGTTCACCGACAAACAACAG‑ATAAAACAAAAGGCCCAGTCTTCCGAc < 2:931279/150‑1 (MQ=38) ttATGGCGGGCGTCC‑TGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACG‑TTCAAATCCGCTCCCGGC‑GGATTTGTCCTACT‑CGGGAGAGTGTTCACCGACAAACAACAG‑ATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGttt < 2:756714/150‑1 (MQ=38) gcgggcgTCC‑TGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACG‑TTCAAATCCGCTCCCGGC‑GGATTTGTCCTACT‑CGGGAGAGTGTTCACCGACAAACAACAG‑ATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTg < 2:259070/151‑1 (MQ=38) gcgTCC‑TGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACG‑TTCAAATCCGCTCCCGGC‑GGATTTGTCCTACT‑CGGGAGAGTGTTCACCGACAAACAACAG‑ATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGt < 1:836551/151‑1 (MQ=39) cccGCCACCCTCCGGGCCGTTGCTTCGC‑AACG‑TTCAAATCCGCTCCCGGC‑GGATTTGTCCTACT‑CGGGAGAGTGTTCACCGACAAACAACAG‑ATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGt > 2:307990/1‑151 (MQ=40) | TTTGCGCATCCTAAATCAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGCGTCC‑TGCCCGCCACCCTCCGGGCCGTTGCTTCGC‑AACG‑TTCAAATCCGCTCCCGGC‑GGATTTGTCCTACT‑CGGGAGAGTGTTCACCGACAAACAACAG‑ATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGT > NC_000913/3423138‑3423434 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |