Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,743,657	0	A	C	42.2%	104.6 / 29.6	64	T309P (ACC→CCC)	yiaK	2,3‑diketo‑L‑gulonate reductase, NADH‑dependent
Reads supporting (aligned to +/- strand):  ref base A (30/7);  new base C (0/27);  total (30/34)
Fisher's exact test for biased strand distribution p-value = 7.46e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCACAAATTTTTATTGCCATTGAAGTGGACAAGCTTATCGACGGTCCCACCCGCGATGCCAA‑GCTGCAACGCATCATGGATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCC‑ATGAATTTACTACCCTGCTGGCCG‑AAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTGGACATATCGCGCAGCCCAATCCGTGCCGTTTGCCCGCCGCCATTGAAA  >  NC_000913/3743510‑3743800                                                                                                                                                      |                                                                                                                                                 tCACAAATTTTTATTGCCATTGAAGTGGACAAGCTTATCGACGGTCCCACCCGCGATGCCAA‑GCTGCAACGCATCATGGATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCC‑ATGAATTTACTAccc                                                                                                                                               >  2:774834/1‑151 (MQ=255)  cacaAATTTTTATTGCCATTGAAGTGGACAAGCTTATCGACGGTCCCACCCGCGATGCCAA‑GCTGCAACGCATCATGGATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCC‑ATGAATTTACTAccc                                                                                                                                               <  1:284882/150‑1 (MQ=255)  cacaAATTTTTATTGCCATTGAAGTGGACAAGCTTATCGACGGTCCCACCCGCGATGCCAA‑GCTGCAACGCATCATGGATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCC‑ATGAATTTACTACCct                                                                                                                                              <  2:992214/151‑1 (MQ=255)  cacaAATTTTTATTGCCATTGAAGTGGACAAGCTTATCGACGGTCCCACCCGCGATGCCAA‑GCTGCAACGCATCATGGATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCC‑ATGAATTTACTACCct                                                                                                                                              <  2:442080/151‑1 (MQ=255)    caAATTTTTATTGCCATTGAAGTGGACAAGCTTATCGACGGTCCCACCCGCGATGCCAA‑GCTGCAACGCATCATGGATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCC‑ATGAATTTACTACCctgc                                                                                                                                            <  2:468891/151‑1 (MQ=255)     aaaTTTTTATTGCCATTGAAGTGGACAAGCTTATCGACGGTCCCACCCGCGATGCCAA‑GCTGCAACGCATCATGGATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCC‑ATGAATTTACTACCctgct                                                                                                                                           <  1:744093/151‑1 (MQ=255)         ttttATTGCCATTGAAGTGGACAAGCTTATCGACGGTCCCACCCGCGATGCCAA‑GCTGCAACGCATCATGGATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCC‑ATGAATTTACTACCCTGCTGGcc                                                                                                                                       >  1:112378/1‑151 (MQ=255)          tttATTGCCATTGAAGTGGACAAGCTTATCGACGGTCCCACCCGCGATGCCAA‑GCTGCAACGCATCATGGATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCC‑ATGAATTTACTACCCTGCTGGCCg                                                                                                                                      >  1:440446/1‑151 (MQ=255)           ttATTGCCATTGAAGTGGACAAGCTTATCGACGGTCCCACCCGCGATGCCAA‑GCTGCAACGCATCATGGATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCC‑ATGAATTTACTACCCTGCTGGCCg                                                                                                                                      <  2:510694/150‑1 (MQ=255)               tGCCATTGAAGTGGACAAGCTTATCGACGGTCCCACCCGCGATGCCAA‑GCTGCAACGCATCATGGATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCC‑ATGAATTTACTACCCTGCTGGCCG‑AAAAc                                                                                                                                >  2:834251/1‑151 (MQ=255)               tGCCATTGAAGTGGACAAGCTTATCGACGATCCCACCCGCGATGCCAA‑GCTGCAACGCATCATGGATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCC‑ATGAATTTACTACCCTGCTGGCCG‑aaaa                                                                                                                                 <  1:277007/150‑1 (MQ=255)                 ccATTGAAGTGGACAAGCTTATCGACGGTCCCACCCGCGATGCCAA‑GCTGCAACGCATCATGGATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCC‑ATGAATTTACTACCCTGCTGGCCG‑AAAAccg                                                                                                                              >  2:784042/1‑151 (MQ=255)                        aGTGGACAAGCTTATCGACGGTCCCACCCGCGATGCCAA‑GCTGCAACGCATCATGGATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCC‑ATGAATTTACTACCCTGCTGGCCG‑AAAACCGCCGTAAc                                                                                                                       >  1:154771/1‑151 (MQ=255)                              cAAGCTTATCGACGGTCCCACCCGCGATGCCAA‑GCTGCAACGCATCATGGATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCC‑ATGAATTTACTACCCTGCTGGCCG‑AAAACCGCCGTAACGGCATc                                                                                                                 >  2:63617/1‑151 (MQ=255)                                aGCTTATCGACGGTCCCACCCGCGATGCCAA‑GCTGCAACGCATCATGGATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCC‑ATGAATTTACTACCCTGCTGGCCG‑AAAACCGCCGTAACGGCATCAc                                                                                                               >  2:684896/1‑151 (MQ=255)                                    tATCGACGGTCCCACCCGCGATGCCAA‑GCTGCAACGCATCATGGATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCC‑ATGAATTTACTACCCTGCTGGCCG‑AAAACCGCCGTAACGGCATCACTGtt                                                                                                           >  1:165963/1‑151 (MQ=255)                                           ggcccccccCGCGATGCAAA‑GCTGCAACGCATCATGGTTTACGTTACTAGTGCGGGGCGTGCTGACAAAAATCAGGCCTTCCGCTTCCCCGCCC‑ATGAATTTACTCCCCTGCGGGCCA‑AAAACCGCCGTAACGGCATCACTGTTGATGACa                                                                                                    <  1:784935/148‑1 (MQ=255)                                           ggcccccccCGCGA‑GCCAAGGCGCCACCGCATCTTGGATTCCTTTCCTGGGCCCGGGCGTGCTGACAAAAACCAGCCCTTTCCCTTCCCCGGCC‑TTGATTTTACTCCCCTGCTGGCAA‑AAAACCGCCGTAACGGCATCACTGTTGATGACa                                                                                                    <  2:285189/148‑1 (MQ=255)                                           ggTCCCACCCGCGATGCCAA‑GCTGCAACGCATCATGGATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCC‑ATGAATTTACTACCCTGCTGGCCG‑AAAACCGCCGTAACGGCATCACTGTTGATGAc                                                                                                     >  2:355625/1‑150 (MQ=255)                                                cACCCGCGATGCCAA‑GCTGCAACGCATCATGGATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCC‑ATGAATTTACTACCCTGCTGGCCG‑AAAACCGCCGTAACGGCATCACTGTTGATGACAGCgtg                                                                                               >  1:490538/1‑151 (MQ=255)                                                 aCCCGCGATGCCAA‑GCTGCAACGCATCATGGATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCC‑ATGAATTTACTACCCTGCTGGCCG‑AAAACCGCCGTAACGGCATCACTGTTGATGACAGCgtgt                                                                                              >  1:393895/1‑151 (MQ=255)                                                  cccGCGAGGCCAA‑GCTGCAACGCATCATGGATTACTTTACTAGTGCCGGGGGTGCTGACAAAAATCAGCCCATCCGCTTCCCCGGCC‑ATAAATTTACTCCCCGGCGGGCCA‑AAAACCGCCGTAACGGCATCACTGTTGATGACAGCGtgtg                                                                                             <  1:831059/151‑1 (MQ=255)                                                   ccGCGATGCCAA‑GCTGCACCGCATCATGGATTCCGTTACTAGTGCCGGGCGTGCTGACAAAAATCAGGCCATTCCCTTCCCCGGCC‑ATGATTTTACTCCCCGGCTGGCCA‑AAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTgg                                                                                            <  1:193301/151‑1 (MQ=255)                                                       gATGCCAA‑GCTGCAACGCATCATGGATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCC‑ATGAATTTACTACCCTGCTGGCCG‑AAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGcc                                                                                         >  2:621472/1‑150 (MQ=255)                                                           ccAA‑GCTGCAACGCATCATGGATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCC‑ATGAATTTACTACCCTGCTGGCCG‑AAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAAt                                                                                    >  2:921607/1‑151 (MQ=255)                                                           caaa‑GCTCCAACGCATCGTGTTTTCCGTTACTGGGGCGGGGGGTGCTGACAAAAATCAGGCTTTCCGCTTCCCCGCCC‑ATAATTTTCCTCCCCTGCTGGCCA‑AAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAAt                                                                                    <  2:798198/149‑1 (MQ=255)                                                             aa‑GCTGCACCGCATCATGGATTACGTTCCTAGTCCCGGGGGTGTTAACAAAAATCGGCCCATCCGTTTCCCCGGCC‑ATAATTTTCCTCCCCTGCTGGCCA‑AAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATc                                                                                   <  1:1757/150‑1 (MQ=255)                                                              a‑GCTGCAACGCATCATGGATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCC‑ATGAATTTACTACCCTGCTGGCCG‑AAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATcc                                                                                  >  1:342512/1‑150 (MQ=255)                                                                  tGCAACACATCATGGTTTACTTTACTAGCGCCGAGCGTGCTGACGAAAACCAGCCCTTTCGCTTCCCCGGCC‑AAGAATTTACTCCCCTGCTGGCCG‑AAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATcc                                                                                  <  2:555826/147‑1 (MQ=255)                                                                     aaCGCATCATGGTTTACGTTACTAGTGCCGGGCGTGCTGACGAAAATCAGGCCATTCGCTTCCCCGGCC‑ATAAATTTACTCCCCTGCTGGCCA‑AAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAgg                                                                               <  1:712057/147‑1 (MQ=255)                                                                          atcatGGTTTGCGTTACTGGTGCCGGGCGTGCTGACGAAAACCAGCCCACCCGCTTCCCCGGCT‑ATAATTTTCCCCCCCTGC‑GGCCGAAAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGtt                                                                           <  1:943118/146‑1 (MQ=255)                                                                               ggATTACTTTACTGGTGCCGGGGGTGCTGACAAAAATCAGCCCATTCCCTT‑CCCGGCCAATAATTTTCCTCCCCTGCTGGCCA‑AAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATgagga                                                                    <  1:181920/148‑1 (MQ=255)                                                                                 aTTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCC‑ATGAATTTACTACCCTGCTGGCCG‑AAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATa                                                                >  1:1005726/1‑150 (MQ=255)                                                                                  ttcctttcctggTGCCGGGCGTCCTAACAAAAATCGGCCCATCCGCTCCCCCGCCC‑ATGATTTTACTCCCCGGCGGGCCG‑AAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGAt                                                                 <  1:729502/137‑1 (MQ=255)                                                                                  ttccGTTACTGGTGCCGGGCGTGCTGACAAAAACCAGCCCTTCCGCTTCCCCGCCC‑ATGAATTTCCTCCCCTGCGGGCCA‑AAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATaa                                                               <  1:742446/147‑1 (MQ=255)                                                                                  ttACGTTACTAGTGCCGAGCGTGCTGAAGAAAATCAGGCCATTCGCTTCCCCGGCA‑ATGAATTTCCTCCCCTGCTGGCCA‑AAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATa                                                                <  1:431483/149‑1 (MQ=255)                                                                                  ttACGTTACTAGGGCCGAGCGTGCTGAAAAAAACCGGCCCATTCGCTTCCCCGGCC‑ATGATTTTCCTCCCCTGCTGGCCA‑AAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATgagga                                                                    <  1:693982/145‑1 (MQ=255)                                                                                    aCGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCC‑ATGAATTTACTACCCTGCTGGCCG‑AAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTc                                                            >  1:1027672/1‑151 (MQ=255)                                                                                     cGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCC‑ATGAATTTACTACCCTGCTGGCCG‑AAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTc                                                            >  2:547765/1‑150 (MQ=255)                                                                                       ttACAAGTGCGGGGGGTGTTGAAAAAAAACGGGCCATTCTCTTACCGGCCT‑ATAATTTTACTCCCCTGCTGGCAA‑AAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCAt                                                          <  1:962470/150‑1 (MQ=255)                                                                                          cTAGGGCCGGGCGGGCTGAAAAAAATCGGACCATTCGCTTCCCCGGCC‑ATGAATTTACTCCCCTGCGGGCAG‑AAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCa                                                           <  1:222933/146‑1 (MQ=255)                                                                                            aGTGCCGGGCGTGCTGACAAAAATCAGGCAATTCGCTTCCCCGGCC‑ATGATTTTACTCCCCTGCGGGCCA‑AAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCAt                                                          <  2:450056/145‑1 (MQ=255)                                                                                             gTGCCGAGCGTCCAAACAAAACACAGCCCCTTCGTTTCCCGGGCC‑TAGATTTTCCTCCCCGGTTGCCCA‑CAACCCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGAtttt                                                    <  2:181814/150‑1 (MQ=255)                                                                                                ccGGGCGTGCTGACAAAAATCAGCCCTTTCGCTTCCCCGGCC‑ATGATTTTACTCCCCTGCTGGCCA‑AAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGAttt                                                     <  2:279078/146‑1 (MQ=255)                                                                                                ccGGGCGTGATGAAAAAAACCAGCCCATTCGCTTCCCCGCCC‑ATAATTTTCCTCCCCTGCGGGCCA‑AAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTgg                                                 <  2:337058/150‑1 (MQ=255)                                                                                                    gcggggtGAAGAAAACCGGCCCTTCCGCTCCCCCGCCA‑ATGATTTTCCCCCCCTGCGGGCGA‑AAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTGGACat                                             <  1:1063174/144‑1 (MQ=255)                                                                                                       tGCTGACAAAAATCGGGCCATCCCTTTCCCCGGCA‑ATAATTTTCCTCCCCGGCTGCCCA‑AAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTGGACata                                            <  2:1026980/148‑1 (MQ=255)                                                                                                           gACGAAAATCAGGCCATTCGCTTACCCGGCC‑ATGAATTTACTACCCTGCTGGCCG‑AAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTGGACATATCgcgc                                      >  1:433328/1‑150 (MQ=255)                                                                                                            acaaaaaCCAGCCCTTTCCCTCCCCCGGCC‑ATGAATTTACTCCCCGGCTGGCCA‑AAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTGGACATATCGCGCa                                     <  1:778987/147‑1 (MQ=255)                                                                                                            aCGAAAATCAGGCCATTCGCTTACCCGGCC‑ATGAATTTACTACCCTGCTGGCCG‑AAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTGGACATATCgcgc                                      >  1:815241/1‑149 (MQ=255)                                                                                                               aaaaTCAGGCCATTCGCTTACCCGGCC‑ATGAATTTACTACCCTGCTGGCCG‑AAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTGGACATATCGCGCAGccc                                 >  1:361319/1‑151 (MQ=255)                                                                                                               aaaaCCAGCCCTTTCCTTTCCCCGCCC‑ATGATTTTACTCCCCTGCGGGCAA‑AAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTGGACATATCGCGCAGc                                   <  1:649478/149‑1 (MQ=255)                                                                                                                aaaTCAGGCCATTCGCTTACCCGGCC‑ATGAATTTACTACCCTGCTGGCCG‑AAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTGGACATATCGCGCAGccc                                 >  2:122007/1‑150 (MQ=255)                                                                                                                aaaTCAGGCCATTCGCTTACCCGGCC‑ATGAATTTACTACCCTGCTGGCCG‑AAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTGGACATATCGCGCAGccc                                 >  2:564774/1‑150 (MQ=255)                                                                                                                    cAGGCCATTCGCTTACCCGGCC‑ATGAATTTACTACCCTGCTGGCCG‑AAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTGGACATATCGCGCAGCCCAATcc                            >  1:491795/1‑151 (MQ=255)                                                                                                                          aTTCGCTTACCCGGCC‑ATGAATTTACTACCCTGCTGGCCG‑AAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTGGACATATCGCGCAGCCCAATCCGTGCCg                      >  1:986104/1‑151 (MQ=255)                                                                                                                                 tACCCGGCC‑ATGAATTTACTACCCTGCTGGCCG‑AAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTGGACATATCGCGCAGCCCAATCCGTGCCGTTTGccc               >  1:311621/1‑151 (MQ=255)                                                                                                                                   cccGGCC‑ATGATTTTACTCCCCTGCTGGCCA‑AAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTGGACATATCGCGCAGCCCAATCCGTGCCGTTTGCccg              <  1:551051/150‑1 (MQ=255)                                                                                                                                      ggCC‑ATGAATTTACTACCCTGCTGGCCG‑AAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTGGACATATCGCGCAGCCCAATCCGTGCCGTTTGCCcgccgc          >  1:431933/1‑151 (MQ=255)                                                                                                                                      ggCC‑ATGAATTTACTACCCTGCTGGCCG‑AAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTGGACATATCGCGCAGCCCAATCCGTGCCGTTTGCCcgccgc          >  1:240376/1‑151 (MQ=255)                                                                                                                                        cc‑ATGAATTTACTACCCTGCTGGCCG‑AAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTGGACATATCGCGCAGCCCAATCCGTGCCGTTTGCCCgccgcc         >  1:306088/1‑150 (MQ=255)                                                                                                                                         c‑ATGAATTTACTACCCTGCTGGCCG‑AAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTGGACATATCGCGCAGCCCAATCCGTGCCGTTTGCCCGCCGCCAt       >  1:792905/1‑151 (MQ=255)                                                                                                                                                tttCCTCCCCTGCTGCCCA‑AAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTGGACATATCGCGCAGCCCAATCCGTGCCGTTTGCCCGCCGCCATTGaaa  <  1:773004/150‑1 (MQ=255)                                                                                                                                                tttACCCCCCTGGGGGCGG‑AAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTGGACATATCGCGCAGCCCAATCCGTGCCGTTTGCCCGCCGCCATTGa    <  1:744306/148‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                 TCACAAATTTTTATTGCCATTGAAGTGGACAAGCTTATCGACGGTCCCACCCGCGATGCCAA‑GCTGCAACGCATCATGGATTACGTTACTAGTGCCGAGCGTGCTGACGAAAATCAGGCCATTCGCTTACCCGGCC‑ATGAATTTACTACCCTGCTGGCCG‑AAAACCGCCGTAACGGCATCACTGTTGATGACAGCGTGTGGGCCAAAATCCAGGCGTTATGAGGAGATAAGTCATGATTTTTGGACATATCGCGCAGCCCAATCCGTGCCGTTTGCCCGCCGCCATTGAAA  >  NC_000913/3743510‑3743800

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑