Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	503,820	0	T	C	41.5%	55.1 / 12.6	41	K293E (AAA→GAA)	fsr	putative fosmidomycin efflux system protein
Reads supporting (aligned to +/- strand):  ref base T (18/6);  new base C (0/17);  total (18/23)
Fisher's exact test for biased strand distribution p-value = 6.89e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCGTAGACCAGAATGGCAGAGAATGCCGAAGCGAGGATAAATCCAATAATCACCGTTAAAACCCCCGTCCAGTGCAGGCTGGCGTAGGGTAAAATCAGCGTAAACGGCGCAACGCCGAGGATAGAGCCCCAAATCACATATTTCCGCCCAATTTTATCCCC‑TACAGG‑CCCGCCGATCACCGTACCTGCCGCAACGGCAAACAGGAAGGCAAACAGATGAAGCTGAGCATTCTGGATAGATAATCCGAATTTTTGCATCAGATAAAAGGTGTAATAGCTGCTG  >  NC_000913/503677‑503959                                                                                                                                                |                                                                                                                                              aGCGTAGACCAGAATGGCAGAGAATGCCGAAGCGAGGATAAATCCAATAATCACCGTTAAAACCCCCGTCCAGTGCAGGCTGGCGTAGGGTAAAATCAGCGTAAACGGCGCAACGCCGAGGATAGAGCCCCAAATCACATATTTCCGccc                                                                                                                                         >  2:476423/1‑150 (MQ=255)  gCGTAGACCAGAATGGCAGAGAATGCCGAAGCGAGGATAAATCCAATAATCACCGTTAAAACCCCCGTCCAGTGCAGGCTGGCGTAGGGTAAAATCAGCGTAAACGGCGCAACGCCGAGGATAGAGCCCCAAATCACATATTTCCGCCCa                                                                                                                                        >  2:547483/1‑150 (MQ=255)                ggCAGAGAATGCCGAAGCGAGGATAAATCCAATAATCACCGTTAAAACCCCCGTCCAGTGCAGGCTGGCGTAGGGTAAAATCAGCGTAAACGGCGCAACGCCGAGGATAGAGCCCCAAATCACATATTTCCGCCCAATTTTATCCCC‑TACa                                                                                                                        <  1:452696/151‑1 (MQ=255)                   agagAATGCCGAAGCGAGGATAAATCCAATAATCACCGTTAAAACCCCCGTCCAGTGCAGGCTGGCGTAGGGTAAAATCAGCGTAAACGGCGCAACGCCGAGGATAGAGCCCCAAATCACATATTTCCGCCCAATTTTATCCCC‑TACAGG‑c                                                                                                                    <  1:206980/151‑1 (MQ=255)                   agagAATGCCGAAGCGAGGATAAATCCAATAATCACCGTTAAAACCCCCGTCCAGTGCAGGCTGGCGTAGGGTAAAATCAGCGTAAACGGCGCAACGCCGAGGATAGAGCCCCAAATCACATATTTCCGCCCAATTTTATCCCC‑TACAGG‑c                                                                                                                    <  1:290605/151‑1 (MQ=255)                        aTGCCGAAGCGAGGATAAATCCAATAATCACCGTTAAAACCCCCGTCCAGTGCAGGCTGGCGTAGGGTAAAATCAGCGTAAACGGCGCAACGCCGAGGATAGAGCCCCAAATCACATATTTCCGCCCAATTTTATCCCC‑TACAGG‑Cccgcc                                                                                                               >  1:743983/1‑151 (MQ=255)                            cGAAGCGAGGATAAATCCAATAATCACCGTTAAAACCCCCGTCCAGTGCAGGCTGGCGTAGGGTAAAATCAGCGTAAACGGCGCAACGCCGAGGATAGAGCCCCAAATCACATATTTCCGCCCAATTTTATCCCC‑TACAGG‑CCCGCCGATc                                                                                                           >  2:188495/1‑151 (MQ=255)                              aaGCGAGGATAAACCCAATAATCACCGTTAAAACCCCCGTCCAGTGCAGGCTGGCGTAGGGTAAAATCCGCGTAATCGGCCCACCGCAGAGTATAGGGCCCCAAATCCCATATTTCCGCCCAATTTTATCCCC‑TCCAGG‑CCCGCCGa                                                                                                             <  2:944492/147‑1 (MQ=255)                                 cGAGGATAAATCCAATAATCACCGTTAAAACCCCCGTCCAGTGCAGGCTGGCGTAGGGTAAAATCAGCGTAAACGGCGCAACGCCGAGGATAGAGCCCCAAATCACATATTTCCGCCCAATTTTATCCCC‑TACAGG‑CCCGCCGATCACCGt                                                                                                      >  2:507164/1‑151 (MQ=255)                                    ggATAGATCCAATAATCACCGTTAAAACCCCCGTCCAGTGCAGGCTGGCGTAGGGTAAAATCAGCGTAAACGGCGCAACGCCGAGGATAGAGCCCCAAATCACATATTTCCGCCCAATTTTATCCCC‑TACAGG‑CCCGCCGATCACCGTAcc                                                                                                   >  2:1049124/1‑151 (MQ=255)                                                        gTTAAAACCCCCGTCCAGTGCAGGCTGGCGTAGGGTAAAATCAGCGTAAACGGCGCAACGCCGAGGATAGAGCCCCAAATCACATATTTCCGCCCAATTTTATCCCC‑TACAGG‑CCCGCCGATCACCGTACCTGCCGCAACGGCAAACAgg                                                                                >  1:495885/1‑150 (MQ=255)                                                           aaacCCCCCGCCCAGTGCGGGCTGGCGAGGGAAAAAACCAGCGTAACCGGCGCAACCCCGAGGAAAGACCCCAAAACCACATATTCCCCCCAAATTTTACCCCC‑AACAGG‑CCCGCCGATCACCGTACCTGCCGCAACGGCAAACAGGaa                                                                              <  1:641718/149‑1 (MQ=255)                                                                 ccctcccggTGCAGGCTGGCTGGGGGAAAAATACGCCTAAACGCCGCAGCGCCGAGAAAGGACCCCCAAACCCCTTATTCCCGCCCATTTTTTTCCCC‑TCCAGC‑CCCGCCGATCACCGTACCTGCCGCAACGGCAAACAGGAAGGCAAACa                                                                      <  1:266078/143‑1 (MQ=255)                                                                 cccGTCCAGTGCGGGCGGGCGTGGGGTAAAATCACCGTAAACGGCGCAACCCCGAGGATAGACCCCAAACTCACATATTCCCGCCAAATTTTTCCCCC‑TACAGC‑CCCGCCGATCACCGTACCTGCCGCAACGGCAAACAGGAAGGCAAACa                                                                      <  1:670565/151‑1 (MQ=255)                                                                  ccGCCCAGTGCAGGCGGGCGTAGGGAAAAACCAGCGAAACCGGCCCAACGCCGAGGATAGACCCCCAAATCACATATTCCCCCCAAATTTTACCCCC‑TACGGC‑CCCGCCGATCACCGTACCTGCCGCAACGGCAAACAGGAAGGCAAACAg                                                                     <  2:906089/151‑1 (MQ=255)                                                                      ccAGTGCAGGCGGGCGTGGGGAAAAATCAGCGAAAACGGCGCAACGCCGAGGATAGACCCCAAAATCACATATTCCCCCCCAATTTTACCCCC‑TACAGG‑CCCGCCGATCACCGTACCTGCCGCAACGGCAAACAGGAAGGCAAACAGATg                                                                  <  1:598975/150‑1 (MQ=255)                                                                            cAGGCTGGCGTAGGGTAAAATCAGCGTAAACGGCGCAACGCCGAGGATAGAGCCCCAAATCACATATTTCCGCCCAATTTTATCCCC‑TACAGG‑CCCGCCGATCACCGTACCTGCCGCAACGGCAAACAGGAAGGCAAACAGATGAAGCTg                                                            >  1:321623/1‑150 (MQ=255)                                                                                  ggggtggggTAAAAACAGCGAAAACGGCGAAACCCCGAGGATAGAGCCCAAAATCACATATTCCCCCCCAATTTTACCCCC‑TACGGG‑CCCGCCGATCACCGTACCTGCCGCAACGGCAAACAGGAAGGCAAACAGATGAAGCTGAGCa                                                        <  1:612941/142‑1 (MQ=255)                                                                                   gCGTAGGGAAAAATCAGCGAAAACGGCGCAACGCCGAGGATAGACCCCCAAATCACATATTCCCGCCAAATTTTACCCCC‑TACAGG‑CCCGCCGATCACCGTACCTGCCGCAACGGCAAACAGGAAGGCAAACAGATGAAGCTGAGCATTCt                                                    <  1:819643/151‑1 (MQ=255)                                                                                     gTAGGTAAAAATCAGCGAAAACGGCGCAACCCCGAGGATAGACCCCAAAATCACATATTCCCGCCAAATTTTATCCCC‑TACAGG‑CCCGCCGATCACCGTACCTGCCGCAACGGCAAACAGGAAGGCAAACAGATGAAGCTGAGCATTCTg                                                   <  1:847758/150‑1 (MQ=255)                                                                                     gTAGGGTAAAATCAGCGTAAACGGCGCAACGCCGAGGATAGAGCCCCAAATCACATATTTCCGCCCAATTTTATCCCC‑TACAGG‑CCCGCCGATCACCGTACCTGCCGCAACGGCAAACAGGAAGGCAAACAGATGAAGCTGAGCATTCTgg                                                  >  2:571003/1‑151 (MQ=255)                                                                                      tAGGGTAAAATCAGCGTAAACGGCGCAACGCCGAGGATAGAGCCCCAAATCACATATTTCCGCCCAATTTTATCCCC‑TACAGG‑CCCGCCGATCACCGTACCTGCCGCAACGGCAAACAGGAAGGCAAACAGATGAAGCTGAGCATTCTgg                                                  >  2:617672/1‑150 (MQ=255)                                                                                              aaTCAGCGTAAACGGCGCAACGCCGAGGATAGAGCCCCAAATCACATATTTCCGCCCAATTTTATCCCC‑TACAGG‑CCCGCCGATCACCGTACCTGCCGCAACGGCAAACAGGAAGGCAAACAGATGAAGCTGAGCATTCTGGATAGATAAt                                         >  2:548391/1‑151 (MQ=255)                                                                                                  aGCGTAAACGGCGCAACGCCGAGGATAGAGCCCCAAATCACATATTTCCGCCCAATTTTATCCCC‑TACAGG‑CCCGCCGATCACCGTACCTGCCGCAACGGCAAACAGGAAGGCAAACAGATGAAGCTGAGCATTCTGGATAGATAATCCg                                      >  2:1056788/1‑150 (MQ=255)                                                                                                  aGCGAAACCGGCGCAACGCCGAGGATAGACCCCCAAACCACATATTCCCCCCAAATTTTACCCCC‑TACGGG‑CCCGCCGATCACCGTACCTGCCGCAACGGCAAACAGGAAGGCAAACAGATGAAGCTGAGCATTCTGGATAGATAATcc                                       <  1:132544/149‑1 (MQ=255)                                                                                                       aaaCGGCGCACCGCCGAGGATAGACCCCAAAACCACATATTCCCCCCCAATTTTACCCCC‑TACGGG‑CCCGCCGATCACCGTACCTGCCGCAACGGCAAACAGGAAGGCAAACAGATGAAGCTGAGCATTCTGGATAGATAATCCGAAtttt                                <  1:960463/151‑1 (MQ=255)                                                                                                       aaaCGGCGCAACGCCGAGGATAGAGCCCTAAATCACATATTTCCGCCCAATTTTATCCCC‑TACAGG‑CCCGCCGATCACCGTACCTGCCGCAACGGCAAACAGGAAGGCAAACAGATGAAGCTGAGCATTCTGGATAGATAATCCGAAtttt                                >  1:1010652/1‑151 (MQ=255)                                                                                                           ggggaaccGCCGAGGATAGGCCCCAAAACCACATTTTCCCCCCCAATTTTACCCCC‑AACAGG‑CCCGCCGATCACCGTACCTGCCGCAACGGCAAACAGGAAGGCAAACAGATGAAGCTGAGCATTCTGGATAGATAATCCGAATTTTTGc                             <  2:296251/143‑1 (MQ=255)                                                                                                           ggCGCACCGCCGAGGATAGGCCCCCAAACCACATTTTCCCCCCAAATTTTACCCCC‑TACGGG‑CCCGCCGATCCCCGTACCTGCCGCAACGGCAAACAGGAAGGCAAACAGATGAAGCTGAGCATTCTGGATAGATAATCCGAATTTTTGCa                            <  1:820569/151‑1 (MQ=255)                                                                                                              gcAACGCCGAGGATAGAGCCCCAAATCACATATTTCCGCCCAATTTTATCCCC‑TACAGG‑CCCGCCGATCACCGTACCTGCCGCAACGGCAAACAGGAAGGCAAACAGATGAAGCTGAGCATTCTGGATAGATAATCCGAATTTTTGCATc                          >  1:678899/1‑150 (MQ=255)                                                                                                                    cggagaATAGACCCCAAAACCCCAT‑TTTCCCCCCCATTTTTCCCCC‑CACCGGCCCCCCCGATCACCGTACCTGCCGCAACGGCAAACAGGAAGGCAAACAGATGAAGCTGAGCATTCTGGATAGATAATCCGAAttttt                               <  2:433768/137‑1 (MQ=255)                                                                                                                    ccGAGGATAGAGCCCCAAATCACATATTTCCGCCCAATTTTATCCCC‑TACAGG‑CCCGCCGATCACCGTACCTGCCGCAACGGCAAACAGGAAGGCAAACAGATGAAGCTGAGCATTCTGGATAGATAATCCGAATTTTTGCATCAGATaa                    >  1:914308/1‑150 (MQ=255)                                                                                                                    ccGAGAATAGAGCCCAAAACCACATATTCCCGCCAAATTTTACCCCC‑TACAGG‑CCCGCCGATCACCGTACCTGCCGCAACGGCAAACAGGAAGGCAAACAGATGAAGCTGAGCATTCTGGATAGATAATCCGAATTTTTGCATCAGATaaa                   <  1:447629/151‑1 (MQ=255)                                                                                                                          atagcccccaAAATCCCATATTCCCCCCAAATTTTATCCCC‑TACGGG‑CCCGCCGATCCCCGTACCTGCCGCAACGGCAAACAGGAAGGCAAACAGATGAAGCTGAGCATTCTGGATAGATAATCCGAATTTTTGCATCAGATaaaa                  <  1:952033/140‑1 (MQ=255)                                                                                                                           tacagCCCCAAACCACAT‑GTTCCCCCAAATTTTAACCCCGAACGGC‑CCCGCAGATCCCCGTACCTGCCGCAACGGCAAACAGGAAGGCAAACAGATGAAGCTGAGCATTCTGGATAGATAATCCGAATTTTTGCATCAGATAAAAGgtg              <  2:563284/146‑1 (MQ=255)                                                                                                                           tAGAGCCCCAAATCACATATTTCCGCCCAATTTTATCCCC‑TACAGG‑CCCGCCGATCACCGTACCTGCCGCAACGGCAAACAGGAAGGCAAACAGATGAAGCTGAGCATTCTGGATAGATAATCCGAATTTTTGCATCAGATAAAAGgtgt             >  1:982369/1‑150 (MQ=255)                                                                                                                               ccccaAAATCACATTTTCCCCCCCAATTTAACCCCC‑AAGGGC‑CCCGCCGATCACCTTACCTGCCGCAACGGCAAACAGGAAGGCAAACAGATGAAGCTGAGCATTCTGGATAGATAATCCGAATTTTTGCATCAGATAAAAGGTGTAATa         <  2:1003359/149‑1 (MQ=255)                                                                                                                                cccaAAATCACATTTTCCCCCCCAATTTTACCCCC‑TACAGG‑CCCGCCGATCACCGTACCTGCCGCAACGGCAAACAGGAAGGCAAACAGATGAAGCTGAGCATTCTGGATAGATAATCCGAATTTTTGCATCAGATAAAAGGTGTAATAg        <  1:253944/150‑1 (MQ=255)                                                                                                                                cccaAAACCCCATTTTCCCGCCCAATTTTACCCCC‑TACGGC‑CCCGCCGATCACCGTACCTGCCGCAACGGCAAACAGGAAGGCAAACAGATGAAGCTGAGCATTCTGGATAGATAATCCGAATTTTTGCATCAGATAAAAGGTGTa            <  1:596767/146‑1 (MQ=255)                                                                                                                                   cAAATCACATATTTCCGCCCAATTTTATCCCC‑TACAGG‑CCCGCCGATCACCGTACCTGCCGCAACGGCAAACAGGAAGGCAAACAGATGAAGCTGAGCATTCTGGATAGATAATCCGAATTTTTGCATCAGATAAAAGGTGTAATAgctgc    >  2:704701/1‑151 (MQ=255)                                                                                                                                      aTCACATATTTCCGCCCAATTTTATCCCC‑TACAGG‑CCCGCCGATCACCGTACCTGCCGCAACGGCAAACAGGAAGGCAAACAGATGAAGCTGAGCATTCTGGATAGATAATCCGAATTTTTGCATCAGATAAAAGGTGTAATAGctgctg  >  1:888398/1‑150 (MQ=255)                                                                                                                                                |                                                                                                                                              AGCGTAGACCAGAATGGCAGAGAATGCCGAAGCGAGGATAAATCCAATAATCACCGTTAAAACCCCCGTCCAGTGCAGGCTGGCGTAGGGTAAAATCAGCGTAAACGGCGCAACGCCGAGGATAGAGCCCCAAATCACATATTTCCGCCCAATTTTATCCCC‑TACAGG‑CCCGCCGATCACCGTACCTGCCGCAACGGCAAACAGGAAGGCAAACAGATGAAGCTGAGCATTCTGGATAGATAATCCGAATTTTTGCATCAGATAAAAGGTGTAATAGCTGCTG  >  NC_000913/503677‑503959

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑