BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,756,894	+TG	coding (1197/1413 nt)	pykF →	pyruvate kinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,756,892	1	.	T	94.3%	456.2 / 18.6	123	coding (1195/1413 nt)	pykF	pyruvate kinase I
Reads supporting (aligned to +/- strand): ref base . (3/4); new base T (62/54); total (65/58)
Fisher's exact test for biased strand distribution p-value = 7.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01
*	NC_000913	1,756,892	2	.	G	94.3%	483.9 / 20.0	123	coding (1195/1413 nt)	pykF	pyruvate kinase I
Reads supporting (aligned to +/- strand): ref base . (3/4); new base G (62/54); total (65/58)
Fisher's exact test for biased strand distribution p-value = 7.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

Only 100 of 125 total aligned reads displayed.

AACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCAC‑‑TGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAA  >  NC_000913/1756742‑1757032

||

aaCCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCAc                                                                                                                                                <  2:1060650/151‑1 (MQ=255)

 aCCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCAC‑‑t                                                                                                                                             <  1:856332/151‑1 (MQ=255)

  ccGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCAC‑‑Tg                                                                                                                                            <  1:1257556/151‑1 (MQ=255)

    gTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGGTGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCAC‑‑Tgtg                                                                                                                                          >  2:187575/1‑149 (MQ=255)

        aCTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCAC‑‑Tgtgacca                                                                                                                                      <  1:1639970/151‑7 (MQ=255)

          tGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCAC‑‑Tgtgaccacc                                                                                                                                    >  2:1060744/1‑143 (MQ=255)

               aTTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGTGACCACCAACg                                                                                                                                  >  1:502685/1‑150 (MQ=255)

                   ccGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGTGACCACCAACGaaaaa                                                                                                                             <  1:1440162/151‑1 (MQ=255)

                   ccGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGTGACCACCAACGaaaa                                                                                                                              >  2:1136226/1‑150 (MQ=255)

                     gAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGCTCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGTGACCACCAACGAAAAAAc                                                                                                                           >  2:943949/1‑151 (MQ=255)

                      aaGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGTGACCACCAACGAAAAAACg                                                                                                                          <  1:951144/151‑1 (MQ=255)

                      aaGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCA‑GGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGTGACCACCAACGAAAAAACgg                                                                                                                         >  2:1902811/1‑151 (MQ=255)

                        gCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGTGACCACCAACGAAAAAACGGc                                                                                                                        <  2:83144/151‑1 (MQ=255)

                         cGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGTGACCACCAACGAAAAAACGGCt                                                                                                                       <  1:780604/151‑1 (MQ=255)

                           gTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGTGACCACCAACGAAAAAACGGCtca                                                                                                                     <  2:402378/151‑1 (MQ=255)

                                 cGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGTGACCACCAACGAAAAAACGGCTCATCAGtt                                                                                                               >  2:109351/1‑151 (MQ=255)

                                  gtggtgCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGTGACCACCAACGAAAAAACGGCTCATCAGTTg                                                                                                              >  1:642084/1‑151 (MQ=255)

                                  gtggtgCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGTGACCACCAACGAAAAAACGGCTCATCAGTTg                                                                                                              >  1:233810/1‑151 (MQ=255)

                                    ggtgCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGTGACCACCAACGAAAAAACGGCTCATCAGTTgg                                                                                                             <  2:1665898/150‑1 (MQ=255)

                                    ggtgCCGTTGAAACTGCTGAAAAACTGAATCCTCCGCTGATCGTGGTTGCTACTCAGGCCGGTAAATCTGCGCGCGCATAACGAAAATACTTCCCGGAGGCCGCCATCC‑GGCACTGTGACCACCAACGAAAAAACGGTTCATCAGTTGGTa                                                                                                           <  1:724685/151‑1 (MQ=255)

                                     gtgCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGTGACCACCAACGAAAAAACGGCTCATCAGTTGGt                                                                                                            >  1:1129285/1‑150 (MQ=255)

                                      tgCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGTGACCACCAACGAAAAAACGGCTCATCAGTTGGTAc                                                                                                          >  1:815676/1‑151 (MQ=255)

                                       gCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACt                                                                                                         <  2:138850/151‑1 (MQ=255)

                                        ccGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTg                                                                                                        >  1:172914/1‑151 (MQ=255)

                                         cGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGa                                                                                                       <  2:483037/151‑1 (MQ=255)

                                          gTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAg                                                                                                      <  2:1914956/151‑1 (MQ=255)

                                             gAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCaa                                                                                                   <  2:1685662/151‑1 (MQ=255)

                                             gAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCaa                                                                                                   <  1:382733/151‑1 (MQ=255)

                                             gAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCa                                                                                                    >  1:1903148/1‑150 (MQ=255)

                                             gAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCa                                                                                                    >  2:1467731/1‑150 (MQ=255)

                                              aaaCTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCaaa                                                                                                  <  2:259542/151‑1 (MQ=255)

                                                aCTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAgg                                                                                                <  1:1645936/151‑1 (MQ=255)

                                                aCTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAgg                                                                                                <  1:634075/151‑1 (MQ=255)

                                                aCTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAgg                                                                                                >  1:942051/1‑151 (MQ=255)

                                                    ctgAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGtt                                                                                            >  1:1535496/1‑151 (MQ=255)

                                                     tgAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAATTCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTtg                                                                                           >  1:577065/1‑151 (MQ=255)

                                                     tgAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTtg                                                                                           >  2:709447/1‑151 (MQ=255)

                                                       aaaaaCTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTtgtg                                                                                         >  1:757587/1‑151 (MQ=255)

                                                       aaaaaCTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTtgtg                                                                                         >  2:1512153/1‑151 (MQ=255)

                                                         aaaCTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGcc                                                                                       <  2:969498/151‑1 (MQ=255)

                                                           aCTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGc                                                                                     >  2:1562958/1‑151 (MQ=255)

                                                           aCTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGc                                                                                     <  2:1096375/151‑1 (MQ=255)

                                                            cTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGc                                                                                     >  1:1880316/1‑150 (MQ=255)

                                                             tGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGATGTGCCGc                                                                                     >  1:1120659/1‑149 (MQ=255)

                                                              ggATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCa                                                                                    <  2:1933562/149‑1 (MQ=255)

                                                              ggATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCa                                                                                    <  1:1175841/149‑1 (MQ=255)

                                                              ggATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGc                                                                                  >  2:315184/1‑151 (MQ=255)

                                                                     ccGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGtt                                                                             <  1:960065/149‑1 (MQ=255)

                                                                      cGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTaaa                                                                          <  1:1583012/151‑1 (MQ=255)

                                                                      cGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTaa                                                                           >  2:547906/1‑150 (MQ=255)

                                                                       gCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTaa                                                                           >  2:1303906/1‑149 (MQ=255)

                                                                        cTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTaa                                                                           >  2:995389/1‑148 (MQ=255)

                                                                          gATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGTGACCAGCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAagag                                                                       <  2:1556939/150‑1 (MQ=255)

                                                                          gATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAagag                                                                       <  2:195784/150‑1 (MQ=255)

                                                                          gATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAaga                                                                        <  2:319884/149‑1 (MQ=255)

                                                                             cGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATc                                                                    <  2:1319871/150‑1 (MQ=255)

                                                                              gTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCAc                                                                  >  2:1816236/1‑151 (MQ=255)

                                                                              gTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCAc                                                                  >  2:700052/1‑151 (MQ=255)

                                                                                ggTTGCTACTCAGGGCGGTAAATCTGCTCGCACAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACtt                                                                <  1:1335677/151‑1 (MQ=255)

                                                                                     cTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACt                                                           >  1:1435047/1‑151 (MQ=255)

                                                                                     cTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACt                                                           >  1:1342263/1‑151 (MQ=255)

                                                                                      tACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGTGCCCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACtg                                                          >  2:1932707/1‑151 (MQ=255)

                                                                                               cGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCt                                                 <  2:48708/151‑1 (MQ=255)

                                                                                                ggTAAATCTGCTCGCGCAGTACGTAAATACTTCCAGGATGCCACCATCCTGGCACTGTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCt                                                 >  1:1186438/1‑150 (MQ=255)

                                                                                                 gTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTAc                                               >  2:1390795/1‑151 (MQ=255)

                                                                                                   aaaTCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCg                                             >  2:31688/1‑151 (MQ=255)

                                                                                                   aaaTCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCg                                             >  1:844989/1‑151 (MQ=255)

                                                                                                       cTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTg                                         >  1:987102/1‑151 (MQ=255)

                                                                                                       cTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTg                                         >  2:1038377/1‑151 (MQ=255)

                                                                                                         gCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTggg                                       >  2:1181705/1‑151 (MQ=255)

                                                                                                         gCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTggg                                       <  1:205678/151‑1 (MQ=255)

                                                                                                         gCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTgg                                        <  1:1879319/150‑1 (MQ=255)

                                                                                                          cTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGt                                      >  1:1197028/1‑151 (MQ=255)

                                                                                                            cgcgCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTaa                                    <  2:620494/151‑1 (MQ=255)

                                                                                                             gcgcAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTaaa                                   <  2:1088106/151‑1 (MQ=255)

                                                                                                                cAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGa                                 >  1:1819435/1‑150 (MQ=255)

                                                                                                                cAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGa                                 >  2:742130/1‑150 (MQ=255)

                                                                                                                cAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGa                                 >  2:1478513/1‑150 (MQ=255)

                                                                                                                 aGTACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAAc                               >  1:297607/1‑151 (MQ=255)

                                                                                                                   tACGTAAATACTTCCCGGATGCCACCATCCTGGCACTGTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTg                             >  1:1921000/1‑151 (MQ=255)

                                                                                                                    aCGTAAATACTTCCCGGATGCCACCATCCTGGCACTGTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTgg                            >  2:569668/1‑151 (MQ=255)

                                                                                                                    aCGTAAATACTTCCCGGATGCCACCATCCTGGCACTGTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTgg                            <  1:1610597/151‑1 (MQ=255)

                                                                                                                      gTAAATACTTCCCGGATGCCACCATCCTGGCACTGTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGct                          >  2:1129882/1‑151 (MQ=255)

                                                                                                                        aaaTACTTCCCGGATGCCACCATCCTGGCACTGTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGctct                        <  2:1640702/151‑1 (MQ=255)

                                                                                                                        aaaTACTTCCCGGATGCCACCATCCTGGCACTGTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGctct                        <  2:430403/151‑1 (MQ=255)

                                                                                                                        aaaTACTTCCCGGATGCCACCATCCTGGCACTGTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGctc                         <  1:791496/150‑1 (MQ=255)

                                                                                                                         aaTACTTCCCGGATGCCACCATCCTGGCACTGTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTg                       <  1:766954/151‑1 (MQ=255)

                                                                                                                         aaTACTTCCCGGATGCCACCATCCTGGCACTGTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTg                       >  2:1644754/1‑151 (MQ=255)

                                                                                                                          aTACTTCCCGGATGCCACCATCCTGGCACTGTGGCCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGc                      >  1:1115218/1‑151 (MQ=255)

                                                                                                                          aTACTTCCCGGATGCCACCATCCTGGCACTGTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGc                      >  2:1451103/1‑151 (MQ=255)

                                                                                                                             cTTCCCGGATGCCACCATCCTGGCACTGTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGc                      >  1:1329982/1‑148 (MQ=255)

                                                                                                                              ttCCCGGATGCCACCATCCTGGCACTGTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCag                    >  1:1017534/1‑149 (MQ=255)

                                                                                                                                cccGGATGCCACCATCCTGGCACTGTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCg                >  1:882928/1‑151 (MQ=255)

                                                                                                                                   ggATGCCACCATCCTGGCACTGTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTc             <  1:300694/151‑1 (MQ=255)

                                                                                                                                    gATGCCACCATCCTGGCACTGTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAAAGCGGTCt            >  1:1367294/1‑151 (MQ=255)

                                                                                                                                     aTGCCACCATCCTGGCACTGTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTg           >  1:456915/1‑151 (MQ=255)

                                                                                                                                            caTCCTGGCACTGTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGcacaca    <  2:1890180/151‑1 (MQ=255)

                                                                                                                                             aTCCTGGCACTGTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGcacaca    >  2:1297930/1‑150 (MQ=255)

                                                                                                                                             aTCCTGGCACTGTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACaa   <  2:915689/151‑1 (MQ=255)

                                                                                                                                               ccTGGCACTGTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACaaa  <  2:633147/150‑1 (MQ=255)

||

AACCGTAAACTGCGCATTACCGAAGCGGTATGCCGTGGTGCCGTTGAAACTGCTGAAAAACTGGATGCTCCGCTGATCGTGGTTGCTACTCAGGGCGGTAAATCTGCTCGCGCAGTACGTAAATACTTCCCGGATGCCACCATCCTGGCAC‑‑TGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAA  >  NC_000913/1756742‑1757032

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`