Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913599,5760TG44.7% 177.8 / 70.9 123V288G (GTG→GGG) cusAcopper/silver efflux system, membrane component
Reads supporting (aligned to +/- strand):  ref base T (7/61);  new base G (55/0);  total (62/61)
Fisher's exact test for biased strand distribution p-value = 1.42e-27
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 135 total aligned reads displayed.

AACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGATCC‑GGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATCGACAACC  >  NC_000913/599427‑599725
                                                                                                                                                      |                                                                                                                                                      
aaCGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGggtg                                                                                                                                                       <  2:654613/151‑1 (MQ=255)
  cGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGggggggg                                                                                                                                                     >  1:12944/1‑150 (MQ=255)
    cTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGgggggggg                                                                                                                                                    >  1:1004527/1‑148 (MQ=255)
      cgacgaCTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGAt                                                                                                                                                  <  2:1788486/150‑1 (MQ=255)
       gacgacTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGgggggggg                                                                                                                                                    >  1:1237694/1‑145 (MQ=255)
       gacgacTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGgggggggg                                                                                                                                                    >  1:1291526/1‑145 (MQ=255)
        acgacTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGGTcc                                                                                                                                                >  2:1745166/1‑150 (MQ=255)
        acgacTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCCTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGggggggggggt                                                                                                                                                 >  1:444421/1‑144 (MQ=255)
         cgacTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGCGggggggggtcc                                                                                                                                                 >  2:1164050/1‑144 (MQ=255)
         cgacTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCt                                                                                                                                               <  2:816695/150‑1 (MQ=255)
           acTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGgggggggggccgtgcg                                                                                                                                            >  1:1211503/1‑141 (MQ=255)
            cTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGGTCCTg                                                                                                                                              >  2:1919213/1‑148 (MQ=255)
            cTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGATCCTg                                                                                                                                              >  2:1194086/1‑148 (MQ=255)
             tttAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGGTcc                                                                                                                                                >  2:679031/1‑145 (MQ=255)
             tttAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCCGGGGGGGGTTCCTg                                                                                                                                              >  2:1234249/1‑147 (MQ=255)
              ttAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGAt                                                                                                                                          <  2:537202/150‑1 (MQ=255)
                 aTCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGATCCTGCGATCC‑gg                                                                                                                                     >  2:1570459/1‑151 (MQ=255)
                   cacaTCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGATCC‑GGCa                                                                                                                                   <  2:904336/151‑1 (MQ=255)
                    acaTCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGGGGGGGGGGGGTCCCGCGGTCC‑gg                                                                                                                                     >  2:1371898/1‑148 (MQ=255)
                     caTCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGGGGGGGGGGGGGCCGGCGGTCC‑gg                                                                                                                                     >  1:799274/1‑147 (MQ=255)
                     caTCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGAACCCGCGGTCC‑GGCaa                                                                                                                                  >  2:75851/1‑150 (MQ=255)
                      aTCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGGGGGGGGGGGGTCCTGCGGTCC‑GGCaaa                                                                                                                                 >  2:533359/1‑150 (MQ=255)
                       tCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGGTCCTCCGATCC‑GGCaaaa                                                                                                                                >  2:221512/1‑150 (MQ=255)
                        cGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGATCC‑GGCAAAAAc                                                                                                                              <  1:156197/151‑1 (MQ=255)
                          ttttAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGGTCCCGCGGTCC‑GGCGAAAACg                                                                                                                             >  1:952053/1‑150 (MQ=255)
                            ttAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGGGGGGGGGGGGTCCGGCGATCC‑GGCAAAAACGccc                                                                                                                          >  2:1648966/1‑151 (MQ=255)
                             tAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGGGGGGGGGGGGTCCCGCGGTCCGGGCAAAAACAcc                                                                                                                           >  1:1581605/1‑150 (MQ=255)
                             tAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGATCC‑GGCAAAAACGccc                                                                                                                          <  1:563755/150‑1 (MQ=255)
                             tAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGGTCCTGCGGTTC‑GGCCAAAACGccc                                                                                                                          >  1:927972/1‑150 (MQ=255)
                               aaaGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGGGGGGGGGGGGGCCTGCGGTCC‑GGCCAAAAAGccc                                                                                                                          >  1:47423/1‑148 (MQ=255)
                                aaGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGGTCCTGCGGTCC‑GGCAAAAAAGCCCggg                                                                                                                       >  1:696289/1‑148 (MQ=255)
                                 aGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGATCCTGCGATCC‑GGCAAAAAAGccc                                                                                                                          >  2:1916927/1‑146 (MQ=255)
                                   ccAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGATCC‑GGCAAAAACGCCCGAGAAGt                                                                                                                   <  2:434483/151‑1 (MQ=255)
                                   ccAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGATCC‑GGCAAAAACGCCCGAGAAGt                                                                                                                   <  1:942700/151‑1 (MQ=255)
                                   ccAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGAGCCCGCGAGCC‑GGCAAAAAAGCCCCAGaa                                                                                                                     >  1:1003060/1‑149 (MQ=255)
                                    cAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGGTCCTGCGGTCC‑GGCAAAAAAGccc                                                                                                                          >  1:1670559/1‑143 (MQ=255)
                                     aGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGGTCCTGCGGTCC‑GGGAAAAACGccc                                                                                                                          >  1:987539/1‑142 (MQ=255)
                                       tGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGGTCCTGCGGACC‑GGGAAAAAAGccccgggaggg                                                                                                                  >  1:1350962/1‑140 (MQ=255)
                                       tGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGGTCCCGCGATCC‑GGCCAAAAAGCCCgaggaggg                                                                                                                  >  1:1924314/1‑146 (MQ=255)
                                          aaaTGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGATCC‑GGCAAAAACGCCCGAGAAGTGATcgcc                                                                                                            <  1:1120633/151‑1 (MQ=255)
                                              ggCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGGGGGGGGGGGGTCCTGCCATCC‑CGCAAAAAAGCCCGAGGAGGGGTCggcgcc                                                                                                         >  1:713919/1‑150 (MQ=255)
                                               gCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGATCC‑GGCAAAAACGCCCGAGAAGTGATCGCCGCCGt                                                                                                       <  2:1218047/151‑1 (MQ=255)
                                                 gTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGATCCTGCGATCC‑GGCAAAAACGCCCGAGAAGGGATCgccgcc                                                                                                         >  2:1754269/1‑147 (MQ=255)
                                                   gCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGGTCCTGCGGTTC‑GGCAAAAACGCCCCGGCAGGGATCgccgcc                                                                                                         >  1:1825027/1‑145 (MQ=255)
                                                   gCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGGTCCTGCGATCC‑GGCAAAAACGCCCCGGAAGGGATcccc                                                                                                            >  1:257836/1‑142 (MQ=255)
                                                     ccGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGGTCCTGCGAGCC‑GGTGAAAACACCCCCGAAGGGGGCGCCGCCCGGAAg                                                                                                   >  1:436081/1‑149 (MQ=255)
                                                       gTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGATCC‑GGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACa                                                                                               <  1:1065936/151‑1 (MQ=255)
                                                       gTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGGTCCTGCGATCC‑GGCAAAAACGCCCGAGAAGGGATCGCCGCCGTGGAgg                                                                                                  >  2:699501/1‑148 (MQ=255)
                                                       gTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGATCCCGCCATCC‑CGCCCAAAACCCCGAGCAGAGACCGgcgccgcg                                                                                                      >  1:1733029/1‑142 (MQ=255)
                                                        tttATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGTGGTCCTGCGATCC‑GACAAAAACGCCCGAGAAGGGATCGCCGCCGTGAAGGAc                                                                                                >  2:1282703/1‑149 (MQ=255)
                                                         ttATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGATCC‑GGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACaa                                                                                              <  2:1228503/150‑1 (MQ=255)
                                                          tATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGATCC‑GGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACaaa                                                                                             <  1:1136044/150‑1 (MQ=255)
                                                              tGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGATCCTGCGGTCC‑GGCAAAAACGCCCGAGAAGGGATCGCCGCCGTGGAGGAAAAACTgg                                                                                         >  2:1382969/1‑150 (MQ=255)
                                                               gcgcGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGATCC‑GGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGaa                                                                                       <  2:1323425/151‑1 (MQ=255)
                                                               gcgcGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGATCC‑GGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGa                                                                                        <  2:1854739/150‑1 (MQ=255)
                                                                    aTGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGATCC‑GGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCt                                                                                  <  1:254100/151‑1 (MQ=255)
                                                                      gTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGATCC‑GGCAAAAACGCCCGAGAAGGGATCGCCGCCGTGAAGGGCAAACTGGAAACGc                                                                                   >  2:951499/1‑148 (MQ=255)
                                                                        tGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGATCC‑GGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGaa                                                                               <  2:433650/150‑1 (MQ=255)
                                                                        tGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGGTCCTGCGATCC‑CGCCAAAAAGCCCGAGAAGGGATCGCCGCCGTGAAGGACAAAACGGAAACGccg                                                                                 >  1:1128547/1‑146 (MQ=255)
                                                                           gAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGATCC‑GGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAagt                                                                           <  2:1574042/151‑1 (MQ=255)
                                                                           gAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGGTCCTGCGGACC‑GGCAAAAACGCCCGAGGAGTGATCGCCGCCGCGAAGGGCAAACTGGAAAAGCTGaaaa                                                                             >  1:22838/1‑149 (MQ=255)
                                                                            aaGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGATCC‑GGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAagta                                                                          <  1:1286821/151‑1 (MQ=255)
                                                                               gTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGATCCTGCGGTCC‑GGCAAAAACGCCCGAGAAGTGATCGCCGCCGGGAAGGGCAAACCGGGAACGCTGaaaa                                                                             >  1:910017/1‑145 (MQ=255)
                                                                                 ccAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGATCC‑GGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTg                                                                     <  2:1315408/151‑1 (MQ=255)
                                                                                 ccAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGATCC‑GGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTg                                                                     <  1:352758/151‑1 (MQ=255)
                                                                                  cAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGATCC‑GGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGc                                                                    <  2:1267614/151‑1 (MQ=255)
                                                                                  cAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGATCC‑GGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGc                                                                    <  2:731819/151‑1 (MQ=255)
                                                                                    gATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGATCC‑GGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCg                                                                  <  2:1800672/151‑1 (MQ=255)
                                                                                     aTTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGATCCTGCGATCC‑GGCAAAAACGCCCGAGAAGTGATCGCCGCCGGGGAGGACAAACTGGAAAAGCTGAAAAGTAGTCTGCCgg                                                                 >  2:1044858/1‑151 (MQ=255)
                                                                                             ggAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGATCC‑GGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTg                                                         <  1:1728186/151‑1 (MQ=255)
                                                                                               agaTGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGATCC‑GGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGga                                                       <  1:345671/151‑1 (MQ=255)
                                                                                                gaTGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGATCC‑GGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGgag                                                      <  1:1809465/151‑1 (MQ=255)
                                                                                                     gcCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGATCC‑GGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGt                                                 <  1:1171513/151‑1 (MQ=255)
                                                                                                         gggCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGATCC‑GGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTaca                                             <  2:645101/151‑1 (MQ=255)
                                                                                                          ggCTTTGTCGAATCAAACGGCAAAGGAGAAGTGGC‑GGGCGGGGTGGTGACCCTGAGATCA‑GTCAAAACCGCCCGAGAAGTGATTGCCTCCGTGAAGGACAAACTGGACAAGCTGAAAAGTAGTCTGCCGGAAGGAGTGGAGATAGTTatac                                            <  1:556307/151‑4 (MQ=255)
                                                                                                          ggCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGATCC‑GGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTacaa                                            <  2:248405/151‑1 (MQ=255)
                                                                                                             aTTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGATCC‑GGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACAt                                         <  2:359408/151‑1 (MQ=255)
                                                                                                             aTTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGTGATCCTGCGATCC‑GGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTacaaca                                          >  2:1481334/1‑150 (MQ=255)
                                                                                                             aTTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGGGATCCTGCGATCC‑GGCAAAAAAGCCCGAGAAGGGATCGCCGGCGGGAAGGACAAACTGGGAACGCCGGAAAGTAGTCTGCCGGAAGGCGGGGGGATAGTTtcaaaa                                          >  1:875797/1‑148 (MQ=255)
                                                                                                                  cGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGATCC‑GGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGAt                                    <  2:1371285/151‑1 (MQ=255)
                                                                                                                  cGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGGGGTCCTGCGATCC‑GGCAAAAACGCCCGAGAAGGGATCGCCGCCGGGAAGGAAAAACTGGAAAAGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGAt                                    >  2:313888/1‑151 (MQ=255)
                                                                                                                   gAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGATCC‑GGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATc                                   <  2:560550/151‑1 (MQ=255)
                                                                                                                   gAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGATCC‑GGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATc                                   <  1:1789281/151‑1 (MQ=255)
                                                                                                                    aaCTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGATCC‑GGCAAAAACGCCCGAGAAGGGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGGAGTCTGCCGGAAGGCGGGGAGATAGGTACAACATACGATCg                                  >  1:390541/1‑151 (MQ=255)
                                                                                                                    aaCTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGTGATCCTGCGATCC‑GGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAAAGCTGAAAAGGAGGCTGCCGGAAGGCGTGGAGGTAGTTAAAACATACGAttg                                  >  2:1016395/1‑149 (MQ=255)
                                                                                                                      cTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGGGGTGGTCCTTCGATCC‑GGCAAAAACGCCCGAGAAGTGATCGCCGCCGGGAAGGACAAAATGGGAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGGGATAGTTACAACATACGATCGc                                 >  1:794602/1‑150 (MQ=255)
                                                                                                                        aaaCGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGATCC‑GGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGcagc                              <  2:1319289/151‑1 (MQ=255)
                                                                                                                        aaaCGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGATCC‑GGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGcag                               <  1:422709/150‑1 (MQ=255)
                                                                                                                          aCGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGATCC‑GGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGcagcca                            <  1:187867/151‑1 (MQ=255)
                                                                                                                            ggcgaaggcgaaGTGGC‑GGGCGGGGTGGTGATCCTGCGATCC‑GGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGcagccagc                          <  1:792255/151‑1 (MQ=255)
                                                                                                                            ggcgaaggcgaaGTGGC‑GGGCGGGGGGGGGATCCTGCGATCC‑GGCAAAAACGCCCGAGAAGGGATCGCCGCCGGGAAGGACAAACTGGAAAAGCTGAAAAGGAGTCTGCCGGAAGGCGTGGGGATAGTTAAAACATAAGAACGcagccag                           >  2:1473661/1‑150 (MQ=255)
                                                                                                                                aaggcgaagTGGC‑GGGCGGGGTGGTGATCCTGCGATCC‑GGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCAt                      <  2:77122/151‑1 (MQ=255)
                                                                                                                                  ggcgaagTGGC‑GGGCGGGGTGGTGATCCTGCGATCC‑GGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTg                    <  2:1758288/151‑1 (MQ=255)
                                                                                                                                      aagTGGC‑GGGCGGGGTGGTGATCCTGCGATCC‑GGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACcg                <  2:1189940/151‑1 (MQ=255)
                                                                                                                                        gTGGC‑GGGCGGGGTGGTGATCCTGCGATCC‑GGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACcgcg              <  1:135038/151‑1 (MQ=255)
                                                                                                                                        gTGGC‑GGGCGGGGTGGTGATCCTGCGATCC‑GGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCCGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACcgcg              <  1:1505923/151‑1 (MQ=255)
                                                                                                                                                gcgggGTGGTGATCCTGCGATCC‑GGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATCg        >  2:933621/1‑150 (MQ=255)
                                                                                                                                                   gggTGGTGATCCTGCGATCC‑GGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATCGACa     >  1:895675/1‑150 (MQ=255)
                                                                                                                                                    ggtggtGATCCTGCGATCC‑GGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATCGACAAc   <  1:619296/151‑1 (MQ=255)
                                                                                                                                                     gtggtgATCCTGCGATCC‑GGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATCGACAAcc  >  2:1474496/1‑151 (MQ=255)
                                                                                                                                                      |                                                                                                                                                      
AACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGC‑GGGCGGGGTGGTGATCCTGCGATCC‑GGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATCGACAACC  >  NC_000913/599427‑599725

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑