Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,825,0560GA40.0% 146.6 / 44.3 95intergenic (‑119/+84)ves/spycold‑ and stress‑inducible protein/periplasmic ATP‑independent protein refolding chaperone, stress‑induced
Reads supporting (aligned to +/- strand):  ref base G (52/5);  new base A (0/38);  total (52/43)
Fisher's exact test for biased strand distribution p-value = 1.98e-21
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 111 total aligned reads displayed.

CGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTAC‑GTTAGTGGTGATCAGGCTTTTTTTACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATT  >  NC_000913/1824908‑1825205
                                                                                                                                                      |                                                                                                                                                     
cGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTAC‑GTTAGTGGTGATCAGGCTTTTTTTACAACTGTTT‑gagaagaga                                                                                                                                                      >  1:627884/1‑150 (MQ=255)
 gACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTAC‑GTTAGTGGTGATCAGGCTTTTTTTACAACTGTTT‑gagaagagaa                                                                                                                                                     >  1:174749/1‑150 (MQ=255)
    aTTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTAC‑GTTAGTGGTGATCAGGCTTTTTTTACAACTGTTT‑GAGAAGagaaa                                                                                                                                                    >  1:758133/1‑148 (MQ=255)
    aTTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTAC‑GTTAGTGGTGATCAGGCTTTTTTTACAACTGTTT‑GAGAAGagaaa                                                                                                                                                    >  2:549338/1‑148 (MQ=255)
      tttACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTAC‑GTTAGTGGTGATCAGGCTTTTTTTACAACTGTTT‑GAGAAGagaaaagaa                                                                                                                                                >  1:1110655/1‑150 (MQ=255)
       ttACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTAC‑GTTAGTGGTGATCAGGCTTTTTTTACAACTGTTT‑GAGAAGagaaaagaaa                                                                                                                                               >  1:298461/1‑150 (MQ=255)
        tACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTAC‑GTTAGTGGTGATCAGGCTTTTTTTACAACTGTTT‑GAGAAGAGAAAAGAAAAc                                                                                                                                             >  2:1185994/1‑151 (MQ=255)
          cGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTAC‑GTTAGTGGTGATCAGGCTTTTTTTACAACTGTTT‑GAGAAGAGAAAAGAAAAccg                                                                                                                                           >  1:761233/1‑151 (MQ=255)
            cATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTAC‑GTTAGTGGTGATCAGGCTTTTTTTACAACTGTTT‑GAGAAGAGAAAAGAAAAccgc                                                                                                                                          >  2:580967/1‑150 (MQ=255)
             atatCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTAC‑GTTAGTGGTGATCAGGCTTTTTTTACAACTGTTT‑GAGAAGAGAAAAGAAAAccgccg                                                                                                                                        >  1:1404227/1‑151 (MQ=255)
               atCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTAC‑GTTAGTGGTGATCAGGCTTTTTTTACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGAt                                                                                                                                      >  2:1890566/1‑151 (MQ=255)
                 cAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTAC‑GTTAGTGGTGATCAGGCTTTTTTTACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATcc                                                                                                                                    >  2:448548/1‑151 (MQ=255)
                  aaaGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTAC‑GTTAGTGGTGATCAGGCTTTTTTTACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGa                                                                                                                                       <  2:169311/147‑1 (MQ=255)
                  aaaGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTAC‑GTTAGTGGTGATCAGGCTTTTTTTACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATcc                                                                                                                                    >  2:1249846/1‑150 (MQ=255)
                    aGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTAC‑GTTAGTGGTGATCAGGCTTTTTTTACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGt                                                                                                                                 >  2:1145651/1‑151 (MQ=255)
                      tATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTAC‑GTTAGTGGTGATCAGGCTTTTTTTACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTcc                                                                                                                               >  2:1012731/1‑151 (MQ=255)
                           cATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTAC‑GTTAGTGGTGATCAGGCTTTTTTTACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGc                                                                                                                          >  2:412106/1‑151 (MQ=255)
                             tACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTAC‑GTTAGTGGTGATCAGGCTTTTTTTACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCAt                                                                                                                        >  2:546059/1‑151 (MQ=255)
                              aCTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTAC‑GTTAGTGGTGATCAGGCTTTTTTTACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCAtt                                                                                                                       >  1:1276523/1‑151 (MQ=255)
                                 cGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTAC‑GTTAGTGGTGATCAGGCTTTTTTTACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACt                                                                                                                    >  2:1635286/1‑151 (MQ=255)
                                  gCTCCCGGCCCGTTCTTTTCCTTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTAC‑GTTAGTGGTGATCAGGCTTTTTTTACAACTGTTT‑GAGAAGAGAACAGAAAACCGCCGATCCTGTCCACCGCATTACTg                                                                                                                   >  1:1037447/1‑151 (MQ=255)
                                   cccccGGCCCTTTTTTTTCATTGTTATCGTTCAGGAAACGGTGGAGGGTTATCGGCACCTGGGGTGTTTTAC‑GTTAGTGGTGATCAGTTTTTTTTTACAACTGTTT‑GAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTg                                                                                                                   <  2:1043954/148‑1 (MQ=255)
                                    tCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTAC‑GTTAGTGGTGATCAGGCTTTTTTTACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGc                                                                                                                  >  2:355886/1‑150 (MQ=255)
                                        ggCCAGTTCTTTTCTTTTTTATTGTTCAGGTACCCGTGGAGGTTAATCGGCACCT‑GGGTTATTTTCATTTAGGGGTGAGCGGTTTTTTTTTAAAGCTTTTT‑GAGAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGt                                                                                                             <  1:701617/151‑1 (MQ=255)
                                         gCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTAC‑GTTAGTGGTGATCAGGCTTTTTTTACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGt                                                                                                             >  2:589885/1‑150 (MQ=255)
                                          cccTTTTTTTTCTTTTTTATCGTTCAGGTACCCGTGGATGGTTATCGGCAACTGGGGTGATTTAC‑TTTAGGGGTGATCGGGTTTTTTTTACAACTTTTT‑GAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAg                                                                                                           <  2:901897/151‑1 (MQ=255)
                                          accGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGCGATTTAC‑GTTAGGGGTGATCAGGCTTTTTTTACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCaa                                                                                                                >  1:610514/2‑146 (MQ=255)
                                              ttCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTAC‑GTTAGTGGTGATCAGGCTTTTTTTACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTgg                                                                                                        >  2:370338/1‑150 (MQ=255)
                                                 ttttatttGTTATCGTCCAGTAACCCGGGGATGTTTGTCGGAAACTCGGGTGATTTGT‑GTTAGGGGTGATCAGGTTTTTTTTACAATTTTTT‑GAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGa                                                                                                       <  2:1430798/142‑1 (MQ=255)
                                                 ttttCATTTTTTTCGTTCAGTTAACGGTGGATGGTTATCGGCACCGGGGGTGATTTCC‑GTTAGTGTTGATCGGGTTTTTTTAACAACTGTTT‑GAAAAGAAAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGAc                                                                                                      <  1:583495/149‑1 (MQ=255)
                                                 ttttCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTAC‑GTTAGTGGTGATCAGGCTTTTTTTACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACa                                                                                                     >  1:1497328/1‑150 (MQ=255)
                                                  tttCTTTTTTATCGTTCAGGTACCCGTGGTTGTTTATCGGCACCTCGGGTATTTTAC‑GTTAGTGGTGATCAGGTTTTTTTTACAACTGTTG‑GAGAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACaa                                                                                                    <  1:1678624/150‑1 (MQ=255)
                                                    tCATTGTTATCGTTGAGGTAACGGTGGATGGTTATCGGCAACTGGGGTGATTTAC‑GTTAGGGGTGATCGGGTTTTTTTTACAACTGTTG‑GAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGa                                                                                                  <  1:93482/150‑1 (MQ=255)
                                                       ttGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTAC‑GTTAGTGGTGATCAGGCTTTTTTTACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcgg                                                                                              >  2:605132/1‑151 (MQ=255)
                                                        ttttttCGTCCGGGAAACCGGGGGTGTTTATCGCAACCTGGGGTTATTTAC‑GTTAGTGGTGATCAGGTTTTTTTTAC‑ACTGTTTAGAGAAGGGAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcgg                                                                                              <  2:956433/145‑1 (MQ=255)
                                                             tCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTAC‑GTTAGTGGTGATCAGGCTTTTTTTACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGCCt                                                                                        >  2:136295/1‑151 (MQ=255)
                                                               gTTAAGGTACCGGTGGGTGTTTAGCGGAAACGGGGGTGATTTAC‑TTTAGTGGTGACCAGTTTTTTTTAACACCTTTTG‑GAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCtt                                                                                       <  1:1314187/150‑1 (MQ=255)
                                                                ttgaggaaccgggggATGTTTAGGGACAACTGGGGTGTTTTCC‑GTTGGGGGTGATCGGTTTTTTTTTACAACTTTTG‑GAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTc                                                                                         <  1:1046832/134‑1 (MQ=255)
                                                                 tCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTAC‑GTTAGTGGTGATCAGGCTTTTTTTACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTa                                                                                      >  2:1002918/1‑149 (MQ=255)
                                                                      tAACCGTGGATGGTTATCGGCAACTCGGGTGATTTAC‑GTTAGTGGTGATCAGGCTTTTTTTACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGttttt                                                                               >  2:1180149/1‑151 (MQ=255)
                                                                       acccGTGGATGTTTATCGGCACCTGGGGTGATTTAC‑GTTAGGGGTGATCAGTTTTTTTTTACACTTTTTT‑GAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGtttttt                                                                              <  2:1217619/149‑1 (MQ=255)
                                                                       aaCCGTGGATGGTTATCGGCAACTCGGGTGATTTAC‑GTTAGTGGTGATCAGGCTTTTTTTACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGtttttt                                                                              >  2:1783045/1‑151 (MQ=255)
                                                                         cggTGGTTGTTTTTCGGCAACTGGGTTGATTTAC‑GTTAGGGGTGATCAGTTTTTTTTTACAACTGTTT‑GAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTg                                                                             <  1:423626/148‑1 (MQ=255)
                                                                            tGGATGGTTATCGGCAACTCGGGTGATTTAC‑TTTAGTGGTGATCAGGTTTTTTTTACAACTGTTT‑GAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGc                                                                           <  1:603958/149‑1 (MQ=255)
                                                                             ggttgtttATCGGCAACTGGGGTGATTTAC‑TTTAGTGGTGATCAGGTTTTTTTTACAACTGTTT‑GAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGc                                                                           <  1:779331/142‑1 (MQ=255)
                                                                             ggATGGTTATCGGCAACTCGGGTGATTTAC‑GTTAGTGGTGATCAGGCTTTTTTTACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTgg                                                                            >  1:976983/1‑147 (MQ=255)
                                                                                 ggTTATCGGCAACTCGGGCGATTTAC‑GTTAGTGGTGATCAGGCTTTTTTTACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGaaa                                                                      >  1:130518/1‑149 (MQ=255)
                                                                                  ttttacgccatttCGGGTGATTAAC‑GTAGGGGCTGATATGTCTTTTTTTAAAATTGTTT‑GAGAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTTGCTGAAAGa                                                                    <  2:254489/138‑1 (MQ=255)
                                                                                   ttATCGACACCGGGGTTGATTTCC‑GTTAGTGGTGATCGGTTTTTTTTTACAACTTTTT‑GAGAAGAAAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGaaa                                                                      <  1:493080/147‑1 (MQ=255)
                                                                                   ttACCGGCACCCCGGGTTTTTTAC‑GTTGGTGGTCATCAGTTTTTTTTTCCACCTTTTT‑GAAAAGAAAAAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGat                                                                   <  2:1785219/150‑1 (MQ=255)
                                                                                    tATCGGCAACTGGGGTGATTTAC‑GTTAGTGGTGATCAGGTTTTTTTTACAACTTTTT‑GAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGatt                                                                  <  2:718696/150‑1 (MQ=255)
                                                                                        ggCAACTGGGGTGATTTAC‑GTTAGTGGTGATCGGTTTTTTTTTACAATTTTTG‑GAGAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGatta                                                                 <  1:672805/147‑1 (MQ=255)
                                                                                           aaCTCGGGTGATTTAC‑GTTAGTGGTGATCGGGTTTTTTTTACAACTTTTT‑GAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTca                                                             <  2:97816/148‑1 (MQ=255)
                                                                                             cTCGGGTGATTTAC‑GTTAGTGGTGATCAGGCTTTTTTTACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTcagcag                                                         >  1:731579/1‑150 (MQ=255)
                                                                                                ggttgttttAC‑GTTAGGGGTGACCGGTCTTTTTTAAAAGTTGTTA‑AAGAAGAAAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTg                                                      <  2:1745305/144‑1 (MQ=255)
                                                                                                gggTGTTTTAC‑GTTAGTGGTGATCAGTTTTTTTTTACAACTGTTG‑GAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTcagcag                                                         <  1:362881/147‑1 (MQ=255)
                                                                                                gggTGTTTTAC‑GTTAGTGGTGATCAGGTTTTTTTTACACCTGTTT‑GAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTg                                                      <  2:902179/150‑1 (MQ=255)
                                                                                                 ggTGATTTCC‑TTTGGTGGTAACCAGTTTTTTTTACCAATTTTTG‑GAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGt                                                        <  1:65524/147‑1 (MQ=255)
                                                                                                 ggTGATTTAC‑GTTAGGGGTGACCGGTCTTTTTTTACACGTTTTG‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTg                                                      <  1:1509656/149‑1 (MQ=255)
                                                                                                 ggTGATTGAC‑GTTAGTGGAGATAAGGTTTTTTTTGCAATTGTTG‑AAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCa                                                    <  2:947653/151‑1 (MQ=255)
                                                                                                       ttAC‑TTTGGTGGTGATCAGTTTTTTTTTACAATTGTTT‑GAGAAGAAAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCAt                                               <  2:587430/150‑1 (MQ=255)
                                                                                                       ttAC‑TTTAGTGGTGATCGGTTTTTTTTAACAATTTTTT‑GAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCAt                                               <  2:1051648/150‑1 (MQ=255)
                                                                                                       ttAC‑GTTAGTGGTGATCAGGTTTTTTTTACAACTGTTT‑GAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCAt                                               <  1:841385/150‑1 (MQ=255)
                                                                                                        tAC‑GTTAGTGGTGATCAGGCTTTTTTTACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCAttt                                             >  1:718180/1‑151 (MQ=255)
                                                                                                         aC‑GTTAGTGGTGATCAGGCTTTTTTTACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCAtttt                                            >  2:1591439/1‑151 (MQ=255)
                                                                                                         aC‑GTTAGTGGTGATCAGGCTTTTTTTACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCAtttt                                            >  1:1447485/1‑151 (MQ=255)
                                                                                                            gTTAGCGGTGATCAGGCTTTTTTTACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCCGCAGTTGCACGCAattt                                            >  2:703024/1‑149 (MQ=255)
                                                                                                             ttAGTGGTGATCAGGCTTTTTTTACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTAc                                          >  1:1654918/1‑150 (MQ=255)
                                                                                                               aGTGGTGATCAGGCTTTTTTTACAACTGTTT‑GAGAAAAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCtt                                       >  1:369514/1‑151 (MQ=255)
                                                                                                                 tggtgATCAGGCTTTTTTTACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCtttt                                     >  1:265313/1‑151 (MQ=255)
                                                                                                                  gtggaccggttttttttaACAACTGTTT‑GAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGTATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCtt                                       <  2:480274/137‑1 (MQ=255)
                                                                                                                  ggtgATCAGGTTTTTTTTACAATTGTTT‑GAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCtttt                                     <  1:1297301/150‑1 (MQ=255)
                                                                                                                  ggtgATCAGGTTTTTTTTACAACTGTTT‑GAAAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCAttt                                             <  2:1280916/142‑1 (MQ=255)
                                                                                                                    tgaccggttttttttcACACATGTTT‑GAAAAGAGAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCtttt                                     <  1:411009/139‑1 (MQ=255)
                                                                                                                    tgATCAGGCTTTTTTTACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGcc                                  >  1:380634/1‑151 (MQ=255)
                                                                                                                    tgATCAGGCTTTTTTTACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGcc                                  >  2:1238003/1‑151 (MQ=255)
                                                                                                                    tgATCAGGCTTTTTTTACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCCTAAGTTTTTTGGCTGTAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGcc                                  >  2:966872/1‑151 (MQ=255)
                                                                                                                        cAGGCTTTTTTTACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTg                              >  2:444749/1‑151 (MQ=255)
                                                                                                                         aGGCTTTTTTTACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGGTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTgg                             >  1:1625311/1‑151 (MQ=255)
                                                                                                                          gttttttttaACATGTGTTT‑AAAAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTgg                             <  1:448476/147‑1 (MQ=255)
                                                                                                                                ttttACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTTGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCt                      >  1:1899436/1‑151 (MQ=255)
                                                                                                                                 tttACATTTTTTT‑GAGAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCt                      <  1:1237805/150‑1 (MQ=255)
                                                                                                                                 tttACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGtt                        >  1:1508204/1‑148 (MQ=255)
                                                                                                                                    aCAACTGTTT‑GAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCa                  <  2:392495/151‑1 (MQ=255)
                                                                                                                                     cAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTc                   >  2:1493832/1‑149 (MQ=255)
                                                                                                                                     cAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTc                   >  2:796154/1‑149 (MQ=255)
                                                                                                                                        cTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACg              >  2:624043/1‑151 (MQ=255)
                                                                                                                                         tGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGc             >  2:1834036/1‑151 (MQ=255)
                                                                                                                                         tGTTG‑GAGAAGAAAAAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTAAGAc               <  2:553659/149‑1 (MQ=255)
                                                                                                                                         tGTTG‑GAAAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGAc               <  1:986321/149‑1 (MQ=255)
                                                                                                                                            tt‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCtt           >  1:714069/1‑150 (MQ=255)
                                                                                                                                                agaagagaaAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTtct         <  2:58426/149‑1 (MQ=255)
                                                                                                                                                 gaagagaaAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCa       >  1:1414977/1‑150 (MQ=255)
                                                                                                                                                  aagagaaAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGGAGCTTCTCaa      >  1:275599/1‑150 (MQ=255)
                                                                                                                                                  aagagaaAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCaa      >  1:498108/1‑150 (MQ=255)
                                                                                                                                                  aagaaaaAAAAAACCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCaa      <  1:1598935/150‑1 (MQ=255)
                                                                                                                                                  aagaaaaAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCaaa     <  2:1408178/151‑1 (MQ=255)
                                                                                                                                                   agagaaAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTTGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCCGGACGTTCTGTCAGACGCTTCTCaaa     >  1:1874169/1‑150 (MQ=255)
                                                                                                                                                     agaaaagaaaACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAAtt  >  1:927121/1‑151 (MQ=255)
                                                                                                                                                      gaaaagaaaaCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAAtt  >  2:1034496/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
CGACATTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTAC‑GTTAGTGGTGATCAGGCTTTTTTTACAACTGTTT‑GAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATT  >  NC_000913/1824908‑1825205

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑