Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,001,9110GA41.2% 152.6 / 60.4 114intergenic (‑122/+199)fliA/fliCRNA polymerase, sigma 28 (sigma F) factor/flagellar filament structural protein (flagellin)
Reads supporting (aligned to +/- strand):  ref base G (57/10);  new base A (0/47);  total (57/57)
Fisher's exact test for biased strand distribution p-value = 3.20e-22
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 115 total aligned reads displayed.

CCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCAT  >  NC_000913/2001764‑2002061
                                                                                                                                                      |                                                                                                                                                       
ccTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGaa                                                                                                                                                       >  2:673833/1‑150 (MQ=255)
    cAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGG‑TTATTTGGGGGTT‑ACATGTAAATTCC‑AGGCAGaaaaaa                                                                                                                                                   <  1:421980/150‑1 (MQ=255)
    cAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGaaaaaaa                                                                                                                                                  <  2:1908813/151‑1 (MQ=255)
    cAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGaaaaaa                                                                                                                                                   <  1:95954/150‑1 (MQ=255)
     aGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑c                                                                                                                                                >  1:252567/1‑151 (MQ=255)
        ggTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑cccc                                                                                                                                             <  1:383086/151‑1 (MQ=255)
         gTATAGAGTGAATTCCCGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCccg                                                                                                                                            <  2:101985/151‑1 (MQ=255)
         gTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCccg                                                                                                                                            >  2:809364/1‑151 (MQ=255)
         gTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCccg                                                                                                                                            <  1:774002/151‑1 (MQ=255)
           ataGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCccgcc                                                                                                                                          >  1:901595/1‑151 (MQ=255)
             agagTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCgg                                                                                                                                        <  1:26183/151‑1 (MQ=255)
                  gAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCg                                                                                                                                   >  2:817570/1‑151 (MQ=255)
                           aTAAACAGCCCTGTTTTATATGAGTTATCGGCATGATTTTCCGTTTCTGCGGGGTTTTTAATCGGACGATTAGTGGGTGAAAGGGGGGT‑TTTTTTGGGGGTT‑ACAGGAAAATCCC‑AGGCAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCAc                                                                                                                          <  1:711481/151‑1 (MQ=255)
                           aTAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCAc                                                                                                                          >  2:1690001/1‑151 (MQ=255)
                           aTAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCAc                                                                                                                          >  2:487721/1‑151 (MQ=255)
                            tAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACg                                                                                                                         >  1:1612902/1‑151 (MQ=255)
                             aaccaccccTGCTTAATATGATTTACCGGCATGTTTATCTTTTTCTGGAGGTTTTTTAATCGGACAATTAGGGGGTGAAATGGGGGT‑TTTTTTGGGGTTT‑AAAGTTAAATCCC‑AGGAAAAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCAc                                                                                                                          <  2:897020/143‑1 (MQ=255)
                             aaaCAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGt                                                                                                                        >  1:1518520/1‑151 (MQ=255)
                              aaCAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGt                                                                                                                        >  1:338429/1‑150 (MQ=255)
                              aaCAGCCCGGCTTTATAGGAGTTATCGGCATAATTATCCGTTTCGGCAGGGTTTTAAATCGGACTATTGGTGGGTGAAATG‑GGGG‑TTTTTTGGGGGTTAACGGGAAAATTCC‑AGGCAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGtt                                                                                                                       <  2:1105826/151‑1 (MQ=255)
                                cAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGc                                                                                                                     >  2:1196759/1‑151 (MQ=255)
                                 aGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCt                                                                                                                    >  1:1640718/1‑151 (MQ=255)
                                 aGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCt                                                                                                                    >  2:1360915/1‑151 (MQ=255)
                                      tGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACa                                                                                                                >  2:658245/1‑150 (MQ=255)
                                        cGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAATAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAAt                                                                                                             >  2:1672013/1‑151 (MQ=255)
                                        cGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAAt                                                                                                             >  1:522126/1‑151 (MQ=255)
                                              atGAGTTTCGGGCATGTTTACCGGTTTGTGGGGGTTTTTTAAGCGAAGGATTGGTGGGTGAAAGGGGGGT‑TTTTTTGGGGGTT‑ACAGATAATTCCC‑AGGAAGAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAAtt                                                                                                            <  1:746522/146‑1 (MQ=255)
                                                gAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGCTGCTGACAACTTGCGCttt                                                                                                     >  1:1583318/1‑151 (MQ=255)
                                                 aGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCttt                                                                                                     >  2:743809/1‑150 (MQ=255)
                                                  gTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTAt                                                                                                   >  1:1091045/1‑151 (MQ=255)
                                                   ttATGGGCTTTATTACCCTTTTCGTGAGGTTTTTAAATCGGACG‑TTTGGGGGTAAAAAGGGGGGTTTTTTTGGGGGTT‑ACAGGAAAATGCA‑AGGAAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTAt                                                                                                   <  1:494919/150‑1 (MQ=255)
                                                   ttATCGGCATGATTATCCGTTTCTGCGGGTTTTTTAATCGGACGATTAGGGGGTAAAATGAGGGG‑TTTTTGGGGGGTT‑ACAGGAAAATTCC‑AGGCAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTAt                                                                                                   <  2:547170/150‑1 (MQ=255)
                                                   ttATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTAt                                                                                                   >  1:1126621/1‑150 (MQ=255)
                                                   ttATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATg                                                                                                  >  2:1265356/1‑151 (MQ=255)
                                                   ttATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATg                                                                                                  >  2:1148672/1‑151 (MQ=255)
                                                    tATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGt                                                                                                 >  1:25889/1‑151 (MQ=255)
                                                        ggCTTGATTATCCGTTTCTGCAGGGTTTTTAATCGGGCGATTAGGGGGTGAAAGGAGGTG‑TTATTTGGGGTTT‑AAAGGTAAATTCC‑AGGAAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGt                                                                                                 <  1:1738929/147‑1 (MQ=255)
                                                             gATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATg                                                                                        >  2:373877/1‑151 (MQ=255)
                                                               tttcccttttCTGGAGGTTTTTTAACCGGACTTTTGGTGGGAAAAAGGAGGGG‑TTTTTGGGGGGTT‑ACAGGTAAATGCG‑AGGAAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGa                                                                                          <  2:861724/140‑1 (MQ=255)
                                                               ttATCCGTTTCTGCAGGTTTTTTAATCGGACGATTAGTGGGTGAAAGGGGGGT‑TTATTTGGGGGTT‑ACAGGAAAATCCC‑AGGCAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATg                                                                                        <  2:681320/149‑1 (MQ=255)
                                                               ttAGCCGTTTCTGGAGGGTTTTTAAGGGGACGATTTTTGGGTAAAAGGAGTGT‑TTTTTTGGGGGTT‑AAAGAAAAATCCC‑GGAAAAAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGAt                                                                                         <  1:1236681/148‑1 (MQ=255)
                                                                tATCCTTTTCTGCGGGGTTTTCAATCGGAAAATTAGGGGGTAAATTGGGGGT‑TTATTGGGGGGTT‑ACAGGAAAATTCC‑AGCCAAAAAAACC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATgcg                                                                                      <  2:766887/150‑1 (MQ=255)
                                                                tATCCTTTTCGGCAGGGTTTTTAAGCGGACAATTAGTGGGTGAAAGGAGGGT‑TTTTTGGGGGGTT‑ACAGGAAAATCCC‑AGGCAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGa                                                                                          <  2:1615208/146‑1 (MQ=255)
                                                                    cGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGt                                                                                  >  1:902597/1‑150 (MQ=255)
                                                                     gTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTa                                                                                 >  1:9169/1‑150 (MQ=255)
                                                                      tttCTGCAGGTTTTTAAATCGGGCGATAAGTGGGTGAAATGAGGGG‑TTGTTTGGGTTTC‑AAAGGAAAATCCG‑AGACAAAAAAAGC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTa                                                                                 <  2:418047/149‑1 (MQ=255)
                                                                       ttCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAAc                                                                              >  1:1598181/1‑151 (MQ=255)
                                                                         cTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGc                                                                            >  1:175561/1‑151 (MQ=255)
                                                                           gcgggttttttAATGGGACATTTAGGGGGTGAAAGGGGGGT‑TTTTTGGGGGGTT‑AAAGGTAATTCCC‑GGGCAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCt                                                                          <  2:1325782/145‑1 (MQ=255)
                                                                           gCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCt                                                                          >  2:537653/1‑151 (MQ=255)
                                                                              ggttttttAATCGGACGATTAGTGGTTAAAATGAGGGG‑TTATTTGGGGTTT‑ACAGGTAAATCCC‑GGGCAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCtt                                                                         <  2:1123626/146‑1 (MQ=255)
                                                                              gggTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTAt                                                                       >  2:853234/1‑151 (MQ=255)
                                                                              gggTTTTAAATGGGACGATTAGGGGGTGAAAGGAGGGT‑TTTTTTGGGGGTT‑ACAGGTAAATTCC‑AGGAAAAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTAt                                                                       <  1:1713649/151‑1 (MQ=255)
                                                                               ggTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATc                                                                      >  2:1086774/1‑151 (MQ=255)
                                                                                 tttttAATCGGACGATTAGGGGGGAAAATGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATCCC‑AGGCAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATcc                                                                     <  2:1914982/150‑1 (MQ=255)
                                                                                  ttttAATCGGACGATTAGTGGGTGAAATGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCgg                                                                   >  1:1751965/1‑151 (MQ=255)
                                                                                     tAATCGGACGATTAGTGGGTGAAATGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCt                                                                >  1:249285/1‑151 (MQ=255)
                                                                                       aTCGGACGATTAGTGGGTGAAATGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTAc                                                              >  2:946939/1‑151 (MQ=255)
                                                                                          ggACGATTGGTGGGTAAAATGAGGGT‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACa                                                             <  2:264923/149‑1 (MQ=255)
                                                                                          ggACGATTAGGGGGTAAAAGGGGGGT‑TTTTTTGGGGGTT‑ACAGGAAAATTCC‑‑GGCAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACaaa                                                           <  1:1339876/151‑1 (MQ=37)
                                                                                            aggATTAGTGGGTAAAAGGAGGTG‑TTTTTTGGGGGTT‑ACGGTTAATTTCA‑GGGCAAAAAAACC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACaaa                                                           <  1:1514650/147‑1 (MQ=25)
                                                                                            aCGATTAGTGGGTAAAATGAGGGT‑TTATTGGGGGGTT‑ACAGTTAAATCCC‑AGGCAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACaaaa                                                          <  2:365187/150‑1 (MQ=38)
                                                                                             cGATTAGTGGGTGAAATGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAAt                                                        >  2:372290/1‑151 (MQ=255)
                                                                                                ttAGTGGGTAAAAGGGGGGT‑TTTTTTGGGGGTT‑ACAGGTAAATCCC‑AGGAAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAAtg                                                       <  1:1351316/149‑1 (MQ=37)
                                                                                                ttAGGGGGTGAAAGGGGGGG‑TTTTTTGGGGGTT‑ACAGGTAAATTCC‑AGGAAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAAt                                                        <  1:857140/148‑1 (MQ=38)
                                                                                                   gggggTAAAAGGGGGGT‑TTTTTTGGGGGTT‑ACGGATAATTCCC‑AGGCAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACaaaaa                                                         <  1:169646/142‑1 (MQ=25)
                                                                                                   gTGGGTGAAATGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCa                                                   >  2:369645/1‑150 (MQ=255)
                                                                                                     gggTAAAATGGGGGT‑TTTTTTGGGGGTT‑ACAGAAAAATCCC‑AGGCAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAAtgt                                                      <  1:686805/145‑1 (MQ=37)
                                                                                                       gTGAAATGGGGGT‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGAAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAAtt                                               <  2:496437/150‑1 (MQ=255)
                                                                                                         gAAATGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCa                                             >  1:1677536/1‑150 (MQ=255)
                                                                                                          aaaggggggt‑TTATTTGGGGGTT‑AAAGGAAAATCCC‑AGGCAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAAt                                           <  1:487636/145‑1 (MQ=37)
                                                                                                          aaaTGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAAt                                           >  1:1437637/1‑151 (MQ=255)
                                                                                                           aaTGAGGGG‑TTTTTGGGGGGTT‑ACAGGAAAATTCC‑AGGCAGAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATa                                          <  2:1887604/151‑1 (MQ=255)
                                                                                                           aaTGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAAt                                           >  1:1068420/1‑150 (MQ=255)
                                                                                                              gAGGGT‑TTTTTTGGGGGTA‑ACAGGTAATTCCC‑AGGAAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATaaa                                        <  1:1217449/150‑1 (MQ=37)
                                                                                                               aGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAAtt                                      >  2:312008/1‑151 (MQ=255)
                                                                                                                    tttttttGGGGGTT‑ACA‑GAAAATTCCAAGGAAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCa                                   <  1:786369/146‑1 (MQ=25)
                                                                                                                     ttttttGGGGGTT‑AAAGGAAAATCCC‑AGGAAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCaa                                  <  1:1425742/147‑1 (MQ=37)
                                                                                                                     ttATTGGGGGGTT‑ACAGGTAAATCCC‑AGGAAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCa                                   <  2:610422/149‑1 (MQ=255)
                                                                                                                      tATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAAtt                                >  2:1817026/1‑151 (MQ=255)
                                                                                                                      tATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAAtt                                >  2:592209/1‑151 (MQ=255)
                                                                                                                       aTTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTc                               >  1:227654/1‑151 (MQ=255)
                                                                                                                         ttGGGGGTT‑ACAGGTAAATCCC‑AGGAAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCa                              <  1:530834/150‑1 (MQ=255)
                                                                                                                           gggggTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACt                           >  2:1824169/1‑151 (MQ=255)
                                                                                                                             ggttt‑ACAGGTAAATTCC‑AGGAAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTg                         <  2:1133546/148‑1 (MQ=255)
                                                                                                                             gggTA‑AAAGGTAATTCCC‑AGGAAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTg                         <  1:1341162/151‑1 (MQ=37)
                                                                                                                               gTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGt                        >  2:357660/1‑150 (MQ=255)
                                                                                                                                tt‑ACAGTTAAATCCC‑AGGCAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTa                       <  1:1142990/150‑1 (MQ=255)
                                                                                                                                tt‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGt                        >  2:1827792/1‑149 (MQ=255)
                                                                                                                                   acagaaaaaTCCA‑AGGCAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGc                    <  1:1441331/145‑1 (MQ=37)
                                                                                                                                    cAGGAAAGTACC‑AGGCAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAg                      <  2:1505354/148‑1 (MQ=255)
                                                                                                                                     agaaaaaTCCC‑AGGAAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGcc                   <  1:953233/146‑1 (MQ=37)
                                                                                                                                         aatttCA‑AGGAAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATa              <  2:1596805/148‑1 (MQ=255)
                                                                                                                                         aaaTCCC‑AGGAAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAgg                     <  1:1281040/144‑1 (MQ=255)
                                                                                                                                          aaTCCC‑AGGAAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATaa             <  1:537566/151‑1 (MQ=255)
                                                                                                                                              cc‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAagcgc         >  1:516694/1‑151 (MQ=255)
                                                                                                                                               c‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAagcgc         >  1:800408/1‑150 (MQ=255)
                                                                                                                                                   gCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAagcgcagcg     >  1:1412170/1‑151 (MQ=255)
                                                                                                                                                    cAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAagcgcagcgc    >  2:122181/1‑151 (MQ=255)
                                                                                                                                                      gAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCAt  >  2:26405/1‑151 (MQ=255)
                                                                                                                                                      |                                                                                                                                                       
CCTTCAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCAT  >  NC_000913/2001764‑2002061

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑