Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,001,9180AC40.5% 226.0 / 22.5 111intergenic (‑129/+192)fliA/fliCRNA polymerase, sigma 28 (sigma F) factor/flagellar filament structural protein (flagellin)
Reads supporting (aligned to +/- strand):  ref base A (57/9);  new base C (0/45);  total (57/54)
Fisher's exact test for biased strand distribution p-value = 1.96e-22
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 121 total aligned reads displayed.

CAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGC  >  NC_000913/2001768‑2002066
                                                                                                                                                         |                                                                                                                                                     
 aGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑c                                                                                                                                                     >  1:252567/1‑151 (MQ=255)
     gTATAGAGTGAATTCCCGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCccg                                                                                                                                                 <  2:101985/151‑1 (MQ=255)
     gTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCccg                                                                                                                                                 >  2:809364/1‑151 (MQ=255)
     gTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCccg                                                                                                                                                 <  1:774002/151‑1 (MQ=255)
       ataGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCccgcc                                                                                                                                               >  1:901595/1‑151 (MQ=255)
         agagTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCgg                                                                                                                                             <  1:26183/151‑1 (MQ=255)
              gAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCg                                                                                                                                        >  2:817570/1‑151 (MQ=255)
                       aTAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCAc                                                                                                                               >  2:1690001/1‑151 (MQ=255)
                       aTAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCAc                                                                                                                               >  2:487721/1‑151 (MQ=255)
                        tAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACg                                                                                                                              >  1:1612902/1‑151 (MQ=255)
                         aaccaccccTGCTTAATATGATTTACCGGCATGTTTATCTTTTTCTGGAGGTTTTTTAATCGGACAATTAGGGGGTGAAATGGGGGT‑TTTTTTGGGGTTT‑AAAGTTAAATCCC‑AGGAAAAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCAc                                                                                                                               <  2:897020/143‑1 (MQ=255)
                         aaaCAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGt                                                                                                                             >  1:1518520/1‑151 (MQ=255)
                          aaCAGCCTGGCGTTATATGGGTTATCGGCATGTTTATCGGTTTCGGCGGGTTTTTTAATCGGACGATTGGTGGGTGAAAGGGGGGG‑TTTTTTGGGGGTT‑ACGGGTAAATTCC‑AGGCAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGtt                                                                                                                            <  1:628498/151‑1 (MQ=255)
                          aaCAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGt                                                                                                                             >  1:338429/1‑150 (MQ=255)
                          aaCAGCCCGGCTTTATAGGAGTTATCGGCATAATTATCCGTTTCGGCAGGGTTTTAAATCGGACTATTGGTGGGTGAAATG‑GGGG‑TTTTTTGGGGGTTAACGGGAAAATTCC‑AGGCAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGtt                                                                                                                            <  2:1105826/151‑1 (MQ=255)
                            cAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGc                                                                                                                          >  2:1196759/1‑151 (MQ=255)
                             aGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCt                                                                                                                         >  1:1640718/1‑151 (MQ=255)
                             aGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCt                                                                                                                         >  2:1251310/1‑151 (MQ=255)
                             aGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCt                                                                                                                         >  2:1360915/1‑151 (MQ=255)
                                  tGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACa                                                                                                                     >  2:658245/1‑150 (MQ=255)
                                    cGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAATAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAAt                                                                                                                  >  2:1672013/1‑151 (MQ=255)
                                    cGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAAt                                                                                                                  >  1:522126/1‑151 (MQ=255)
                                          atGAGTTTCGGGCATGTTTACCGGTTTGTGGGGGTTTTTTAAGCGAAGGATTGGTGGGTGAAAGGGGGGT‑TTTTTTGGGGGTT‑ACAGATAATTCCC‑AGGAAGAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAAtt                                                                                                                 <  1:746522/146‑1 (MQ=255)
                                            gAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGCTGCTGACAACTTGCGCttt                                                                                                          >  1:1583318/1‑151 (MQ=255)
                                              gTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTAt                                                                                                        >  1:1091045/1‑151 (MQ=255)
                                               ttATGGGCTTTATTACCCTTTTCGTGAGGTTTTTAAATCGGACG‑TTTGGGGGTAAAAAGGGGGGTTTTTTTGGGGGTT‑ACAGGAAAATGCA‑AGGAAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTAt                                                                                                        <  1:494919/150‑1 (MQ=255)
                                               ttATCGGCATGATTATCCGTTTCTGCGGGTTTTTTAATCGGACGATTAGGGGGTAAAATGAGGGG‑TTTTTGGGGGGTT‑ACAGGAAAATTCC‑AGGCAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTAt                                                                                                        <  2:547170/150‑1 (MQ=255)
                                               ttATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTAt                                                                                                        >  1:1126621/1‑150 (MQ=255)
                                               ttATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATg                                                                                                       >  2:1265356/1‑151 (MQ=255)
                                                tATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGt                                                                                                      >  1:25889/1‑151 (MQ=255)
                                                    ggCTTGATTATCCGTTTCTGCAGGGTTTTTAATCGGGCGATTAGGGGGTGAAAGGAGGTG‑TTATTTGGGGTTT‑AAAGGTAAATTCC‑AGGAAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGt                                                                                                      <  1:1738929/147‑1 (MQ=255)
                                                       aTGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCgg                                                                                                >  2:205740/1‑150 (MQ=255)
                                                           ttATCCGTTTCTGCAGGTTTTTTAATCGGACGATTAGTGGGTGAAAGGGGGGT‑TTATTTGGGGGTT‑ACAGGAAAATCCC‑AGGCAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATg                                                                                             <  2:681320/149‑1 (MQ=255)
                                                           ttATCCGTTTCTGCAGGTTTTTTAATCGGACGATTAGGGGGTAAAATGAGGGT‑TTATTTGGGGTTT‑ACAGGTAAATTCC‑AGGAAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCgg                                                                                                <  2:370427/146‑1 (MQ=255)
                                                           ttAGCCGTTTCTGGAGGGTTTTTAAGGGGACGATTTTTGGGTAAAAGGAGTGT‑TTTTTTGGGGGTT‑AAAGAAAAATCCC‑GGAAAAAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGAt                                                                                              <  1:1236681/148‑1 (MQ=255)
                                                            tATCCTTTTCTGCGGGGTTTTCAATCGGAAAATTAGGGGGTAAATTGGGGGT‑TTATTGGGGGGTT‑ACAGGAAAATTCC‑AGCCAAAAAAACC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATgcg                                                                                           <  2:766887/150‑1 (MQ=255)
                                                            tATCCTTTTCGGCAGGGTTTTTAAGCGGACAATTAGTGGGTGAAAGGAGGGT‑TTTTTGGGGGGTT‑ACAGGAAAATCCC‑AGGCAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGa                                                                                               <  2:1615208/146‑1 (MQ=255)
                                                                cGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGt                                                                                       >  1:902597/1‑150 (MQ=255)
                                                                 gTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTa                                                                                      >  1:9169/1‑150 (MQ=255)
                                                                  tttCTGCAGGTTTTTAAATCGGGCGATAAGTGGGTGAAATGAGGGG‑TTGTTTGGGTTTC‑AAAGGAAAATCCG‑AGACAAAAAAAGC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTa                                                                                      <  2:418047/149‑1 (MQ=255)
                                                                     cTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGc                                                                                 >  1:175561/1‑151 (MQ=255)
                                                                       gCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCt                                                                               >  2:537653/1‑151 (MQ=255)
                                                                          ggttttttAATCGGACGATTAGTGGTTAAAATGAGGGG‑TTATTTGGGGTTT‑ACAGGTAAATCCC‑GGGCAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCtt                                                                              <  2:1123626/146‑1 (MQ=255)
                                                                          gggTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTAt                                                                            >  2:853234/1‑151 (MQ=255)
                                                                          gggTTTTAAATGGGACGATTAGGGGGTGAAAGGAGGGT‑TTTTTTGGGGGTT‑ACAGGTAAATTCC‑AGGAAAAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTAt                                                                            <  1:1713649/151‑1 (MQ=255)
                                                                          gggTTTTAAACCGGACGATTGGGGGGTAAAATGGGGGT‑TTATTTGGGGTTT‑ACAGGTAAATTCC‑AGGAAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGc                                                                                 <  1:165997/146‑1 (MQ=255)
                                                                             tttttAATCGGACGATTAGGGGGGAAAATGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATCCC‑AGGCAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATcc                                                                          <  2:1914982/150‑1 (MQ=255)
                                                                              ttttAATCGGACGATTAGTGGGTGAAATGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCgg                                                                        >  1:1751965/1‑151 (MQ=255)
                                                                                taaaTCGGACGATAAGGGGGTAAAAGGAGGGT‑TTATTGGGGGGTT‑AAAGTTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGc                                                                       <  2:768966/148‑1 (MQ=38)
                                                                                 tAATCGGACGATTAGTGGGTGAAATGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCt                                                                     >  1:249285/1‑151 (MQ=255)
                                                                                  aaTCGGACGATTAGTGGGTGAAATGAGGTT‑TTATTGGGGGGTT‑ACAGGTAAATTCC‑AGGCAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCt                                                                     <  2:1100946/150‑1 (MQ=255)
                                                                                   aTCGGACGATTAGTGGGTGAAATGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTAc                                                                   >  2:946939/1‑151 (MQ=255)
                                                                                    acgaaggtttAGTGGGTAAAAGGGGGTT‑TTTTTTGGGGTTT‑ACAGGTAAATTCC‑AGGAAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACa                                                                  <  1:1448989/143‑1 (MQ=37)
                                                                                      ggACGATTGGTGGGTAAAATGAGGGT‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACa                                                                  <  2:264923/149‑1 (MQ=255)
                                                                                      ggACGATTAGGGGGTAAAAGGGGGGT‑TTTTTTGGGGGTT‑ACAGGAAAATTCC‑‑GGCAAAAAAAAACCCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACaaa                                                                <  1:1339876/151‑1 (MQ=37)
                                                                                        aggATTAGTGGGTAAAAGGAGGTG‑TTTTTTGGGGGTT‑ACGGTTAATTTCA‑GGGCAAAAAAACC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACaaa                                                                <  1:1514650/147‑1 (MQ=25)
                                                                                        aCGATTAGTGGGTAAAATGAGGGT‑TTATTGGGGGGTT‑ACAGTTAAATCCC‑AGGCAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACaaaa                                                               <  2:365187/150‑1 (MQ=38)
                                                                                         cGATTAGTGGGTGAAATGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAAt                                                             >  2:372290/1‑151 (MQ=255)
                                                                                            ttAGTGGGTAAAAGGGGGGT‑TTTTTTGGGGGTT‑ACAGGTAAATCCC‑AGGAAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAAtg                                                            <  1:1351316/149‑1 (MQ=37)
                                                                                            ttAGGGGGTGAAAGGGGGGG‑TTTTTTGGGGGTT‑ACAGGTAAATTCC‑AGGAAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAAt                                                             <  1:857140/148‑1 (MQ=38)
                                                                                               gTGGGTGAAATGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCa                                                        >  2:369645/1‑150 (MQ=255)
                                                                                                 gggTAAAATGGGGGT‑TTTTTTGGGGGTT‑ACAGAAAAATCCC‑AGGCAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAAtgt                                                           <  1:686805/145‑1 (MQ=37)
                                                                                                   gTGAAATGGGGGT‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGAAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAAtt                                                    <  2:496437/150‑1 (MQ=255)
                                                                                                     gAAATGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCa                                                  >  1:1677536/1‑150 (MQ=255)
                                                                                                      aaaggggggt‑TTATTTGGGGGTT‑AAAGGAAAATCCC‑AGGCAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAAt                                                <  1:487636/145‑1 (MQ=37)
                                                                                                      aaaTGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAAt                                                >  1:1437637/1‑151 (MQ=255)
                                                                                                       aaTGAGGGG‑TTTTTGGGGGGTT‑ACAGGAAAATTCC‑AGGCAGAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATa                                               <  2:1887604/151‑1 (MQ=255)
                                                                                                       aaTGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAAt                                                >  1:1068420/1‑150 (MQ=255)
                                                                                                           aGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAAtt                                           >  2:312008/1‑151 (MQ=255)
                                                                                                                tttttttGGGGGTT‑ACA‑GAAAATTCCAAGGAAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCa                                        <  1:786369/146‑1 (MQ=25)
                                                                                                                 ttttttGGGGGTT‑AAAGGAAAATCCC‑AGGAAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCaa                                       <  1:1425742/147‑1 (MQ=37)
                                                                                                                 ttATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAAt                                      >  1:448986/1‑151 (MQ=255)
                                                                                                                 ttATTGGGGGGTT‑ACAGGTAAATCCC‑AGGAAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCa                                        <  2:610422/149‑1 (MQ=255)
                                                                                                                  tATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAAtt                                     >  2:1817026/1‑151 (MQ=255)
                                                                                                                  tATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAAtt                                     >  2:592209/1‑151 (MQ=255)
                                                                                                                   aTTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTc                                    >  1:227654/1‑151 (MQ=255)
                                                                                                                      tGGGGGTT‑ATAGGTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGGTGCTGAAAAATTGCGCTTTATGGTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGGGCAAATTCAATAAATTGCAATTCAAc                                 >  1:1281758/1‑151 (MQ=255)
                                                                                                                       gggggTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACt                                >  2:1824169/1‑151 (MQ=255)
                                                                                                                         ggttt‑ACAGGTAAATTCC‑AGGAAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTg                              <  2:1133546/148‑1 (MQ=255)
                                                                                                                            tt‑ACAGTTAAATCCC‑AGGCAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTa                            <  1:1142990/150‑1 (MQ=255)
                                                                                                                            tt‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGt                             >  2:1827792/1‑149 (MQ=255)
                                                                                                                                cAGGAAAGTACC‑AGGCAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAg                           <  2:1505354/148‑1 (MQ=255)
                                                                                                                                 agaaaaaTCCC‑AGGAAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGcc                        <  1:953233/146‑1 (MQ=37)
                                                                                                                                     aatttCA‑AGGAAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATa                   <  2:1596805/148‑1 (MQ=255)
                                                                                                                                     aaaTCCC‑AGGAAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAgg                          <  1:1281040/144‑1 (MQ=255)
                                                                                                                                      aaTCCC‑AGGAAAAAAAAAC‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATaa                  <  1:537566/151‑1 (MQ=255)
                                                                                                                                          cc‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAagcgc              >  1:516694/1‑151 (MQ=255)
                                                                                                                                           caagcaaaaaaaaac‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAagc                <  1:582711/141‑1 (MQ=255)
                                                                                                                                           c‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAagcgc              >  1:800408/1‑150 (MQ=255)
                                                                                                                                              ggaaaaaaaaac‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAagcgcagc           <  1:1527929/146‑1 (MQ=255)
                                                                                                                                               gCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAagcgcagcg          >  1:1412170/1‑151 (MQ=255)
                                                                                                                                                cAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAagcgcagcgc         >  2:122181/1‑151 (MQ=255)
                                                                                                                                                 aaaaaaaac‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAagcgcagcg          <  2:9169/147‑1 (MQ=255)
                                                                                                                                                 aaaaaaaac‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAagcgcagc           <  1:1265149/146‑1 (MQ=255)
                                                                                                                                                 aaaaaaaac‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAgcgcagcgca        <  2:1091235/149‑1 (MQ=255)
                                                                                                                                                  gAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCAt       >  2:26405/1‑151 (MQ=255)
                                                                                                                                                   aaaaaac‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCAt       <  2:627900/150‑1 (MQ=255)
                                                                                                                                                   aaaaaac‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCAt       <  1:1430809/150‑1 (MQ=255)
                                                                                                                                                    aaaaaa‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATc      >  2:893923/1‑150 (MQ=255)
                                                                                                                                                       aaa‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGc  >  1:1149197/1‑151 (MQ=255)
                                                                                                                                                         |                                                                                                                                                     
CAGCGGTATAGAGTGAATTCACGATAAACAGCCCTGCGTTATATGAGTTATCGGCATGATTATCCGTTTCTGCAGGGTTTTTAATCGGACGATTAGTGGGTGAAATGAGGGG‑TTATTTGGGGGTT‑ACAGGTAAATTCC‑AGGCAGAAAAAAA‑CCCCGCCGGTGGCGGGGAAGCACGTTGCTGACAAATTGCGCTTTATGTTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAAAATGTGCAAATTCAATAAATTGCAATTCAACTTGTAGGCCTGATAAGCGCAGCGCATCAGGC  >  NC_000913/2001768‑2002066

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑