Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,434,8820TG42.9% 200.3 / 23.6 112Y252S (TAC→TCC) truAtRNA pseudouridine(38‑40) synthase
Reads supporting (aligned to +/- strand):  ref base T (16/48);  new base G (48/0);  total (64/48)
Fisher's exact test for biased strand distribution p-value = 4.00e-18
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 112 total aligned reads displayed.

GTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACC  >  NC_000913/2434736‑2435028
                                                                                                                                                  |                                                                                                                                                   
gTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATc                                                                                                                                                 <  1:1636509/151‑1 (MQ=255)
  ccATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGGAGATCATACCGGTCAGGGGAAt                                                                                                                                                  >  1:1089211/1‑148 (MQ=255)
  ccATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATcc                                                                                                                                                <  2:1352481/150‑1 (MQ=255)
   cATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATAATACCCGTCAGGGGAAAcc                                                                                                                                                >  1:1749155/1‑149 (MQ=255)
    atatTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATACCCGGCAGGGGAATACAcc                                                                                                                                             >  1:1631820/1‑151 (MQ=255)
    atatTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCAcc                                                                                                                                             <  2:1487509/151‑1 (MQ=255)
        ttGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCCGTCAGGGGAATTCAcccc                                                                                                                                           >  1:721866/1‑147 (MQ=255)
          gCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGGAGATCATACCCGTCAGGGGAATCCACCCCGAcc                                                                                                                                       >  2:1788245/1‑151 (MQ=255)
           ccTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATACCGGGCAGGGTAATACACCCCGaac                                                                                                                                       >  1:963816/1‑148 (MQ=255)
             tATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGGTTTGGAAGATTATACCGGGCAGGGGAATCCCCcccgcc                                                                                                                                        >  1:823904/1‑145 (MQ=255)
              aTGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGGAGATCATAACGGTCAGGGGAATACACCGCGAcc                                                                                                                                       >  1:352469/1‑147 (MQ=255)
                gCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATa                                                                                                                                  <  1:707736/150‑1 (MQ=255)
                   ttGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGGAGATCATACCGGTCAGGGGAATCCACCGCGACCCGATAAA‑Gc                                                                                                                             >  2:175090/1‑151 (MQ=255)
                    tGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑g                                                                                                                              <  2:1419837/149‑1 (MQ=255)
                    tGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGGAATTCACCGCGACCAGATACA‑Gcc                                                                                                                            >  2:1430485/1‑151 (MQ=255)
                       aCTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCt                                                                                                                          <  2:1245322/150‑1 (MQ=255)
                          cGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCg                                                                                                                      <  2:436747/151‑1 (MQ=255)
                          cGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCCGGCAGGGTAATCCACCCCGACCCGATACA‑ACCCCTcc                                                                                                                       >  1:787124/1‑150 (MQ=255)
                             cATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGTCAGGGGAATCCACCCCGACCAGATACA‑GCCCCTCCGCtt                                                                                                                   >  1:156345/1‑151 (MQ=255)
                             cATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGGAGAACATACCCGTCAGGGGAATCCACCGCGACCAGATACA‑GCCCTTCCGCtt                                                                                                                   >  1:778505/1‑151 (MQ=255)
                               tCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGAACCTACCGGTGCGGGGAAACCCACGCGACCCGAGACA‑GACCTTCCGCttt                                                                                                                  >  1:369464/1‑150 (MQ=255)
                                cTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGGAGATCATACCGGTCAGGGGAATCCACCGCGACCAGATACA‑ACCCTTCCGCtttt                                                                                                                 >  2:1089882/1‑150 (MQ=255)
                                 tttCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCACAACGGGCGAGGGAATCCACCCCGACCAGATACA‑GCCCTTCCTCtttt                                                                                                                 >  2:1259109/1‑149 (MQ=255)
                                    cAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCg                                                                                                             <  1:1484462/150‑1 (MQ=255)
                                      ggCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTtg                                                                                                          <  2:1480159/151‑1 (MQ=255)
                                        cTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGGCAGGGGAATCCACCGCGACCAGATACA‑ACCCTTCCGCTTTTTCCGGtgct                                                                                                        >  2:550458/1‑151 (MQ=255)
                                         tGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGGTTTTGGAGATCATACCGGGGAGGGTAATCCACCCCGACCAGGTACA‑GCCCTTCCCCCTTTTCCGtttc                                                                                                         >  1:160580/1‑147 (MQ=255)
                                           tAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGACTATACCCGGCAAGGGAAAACACCGCGAACCGATAAA‑ACCCTTTCGCTTTTTTCCTTGTTgcc                                                                                                     >  2:1726293/1‑151 (MQ=255)
                                            aaCTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTgcc                                                                                                     <  2:1585808/150‑1 (MQ=255)
                                              cTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGGCAGGGGAATTCACCCCGACCAGATACA‑GCCCTTCCGCTTTTTCCGTTGCTgccgcc                                                                                                  >  1:1231069/1‑151 (MQ=255)
                                                aCGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTgccgcc                                                                                                  <  2:829109/149‑1 (MQ=255)
                                                  gTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGTCAGGGGAATCCACCCCGACCCGATACA‑GCCCTTCCGCTTTTTCCGGTGCTGccgcccgc                                                                                               >  1:520010/1‑150 (MQ=255)
                                                  gTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGGCAGGGTAATCCAACGCGACCAGATACA‑ACCCCTCCGCCTTTTCCGTTGCTgccgcc                                                                                                  >  2:1192471/1‑147 (MQ=255)
                                                  gTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCCGGCAGGGGAATCCACCGCGACCCGATACA‑GCCCCTCCGCTTTTGCCGCTGCTgccgcccg                                                                                                >  2:85349/1‑147 (MQ=255)
                                                    tGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGGAATCCACCGCGAACAGATAAA‑GGCCTTCCCCTTTTGGCCTTGCTgccccc                                                                                                  >  2:703700/1‑145 (MQ=255)
                                                     ggCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGAGCATAACGGGGAGGGGAAAACCCCGCGACCCGAAACA‑ACCCCTCCGCCTTTGTCCTTGGTGCCGCCcgcgct                                                                                            >  1:1524715/1‑148 (MQ=255)
                                                         aCAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGTTCATACCCCCCCGGGGAATCCCCCCCGACCCGATACA‑ACCCCTTCGCCTTTGCCGTTGCTGCCGCCCTCGGTCTGTc                                                                                       >  1:111996/1‑151 (MQ=255)
                                                             ccTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGAACATACCGGGCAGGGGAATCCACCGCGACCCGATACA‑GCCCTTCCGCTTTTTCCGGTGTTGCCGCCAGCGGTTTGTCCttt                                                                                   >  2:1005483/1‑151 (MQ=255)
                                                              cTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCt                                                                                     <  2:416096/148‑1 (MQ=255)
                                                               tGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCATACCGGTCAGGGGAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTTCCGTTGCTGCCGCCAGCGTTCTGTCCttt                                                                                   >  2:1862894/1‑149 (MQ=255)
                                                               tGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGGCAGGGGAATCCACCGCGACCAGATACA‑GCCCTTCCCCTTTTGCCGTTGCTGCCCCCAGCGTTCTGTCCttt                                                                                   >  2:1134478/1‑149 (MQ=255)
                                                               tGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGGTTTTGTAGAGTAGAACGGGCAGGGGAATCCCCCCCGACCAGATAAA‑GCCCTTCCGGTTTTGCCGTTTCTGCCGCCCGCGTTTTGTCCttt                                                                                   >  1:1456899/1‑149 (MQ=255)
                                                                 aaaTGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTgccg                                                                               <  2:95083/151‑1 (MQ=255)
                                                                      ttCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGGAATCCACCGCGACCAGAAACA‑GCCCTTCCGCTTTTTCCGTTGCTTCCCCCAGCGGTCTGTCCTTTGccgcccgc                                                                          >  2:1746328/1‑151 (MQ=255)
                                                                        ccGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGGAGATCATAACGGTCAGGGGAAACCACCGCGACCAGATACA‑GCCCCTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGc                                                                          >  2:813967/1‑149 (MQ=255)
                                                                        ccGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCa                                                                         <  2:14974/150‑1 (MQ=255)
                                                                            gCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAActct                                                                    <  1:1895781/151‑1 (MQ=255)
                                                                             cATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGTCAGGGGAATCCACCGCGACCCGATACA‑GCCCTTCCCCTTTTTCCGGTGCTGCCCCCAGCGCTCTGTCCTTTTCCGCCAGCaa                                                                        >  1:292767/1‑146 (MQ=255)
                                                                             cATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGTCAGGGGAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTTCCGTTGCTGCCCCCCGCGTTCTGTCCTTTTCCGCCAGCAActct                                                                    >  2:887129/1‑150 (MQ=255)
                                                                             cATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTg                                                                   <  2:795312/151‑1 (MQ=255)
                                                                             cATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTg                                                                   <  2:543712/151‑1 (MQ=255)
                                                                               ttattCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGTCAGGGGAAACCACCGCGACCCGAAACA‑GCCCTTTCGCTTTTGCCGTTGCTGCCGCCAGCGGTCTGTCCTTTTCCGCCCGCAACTCTGc                                                                  >  1:158862/1‑150 (MQ=255)
                                                                                  ttCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGGCAGGGGAATCCCCCCCGGCCCGATACA‑ATCCTTCCCCTTTTGCCGTTTCTGCCGCCCGCGTTCTGTCCTTTTCCGCCCGCCACCCTGCTAt                                                               >  1:371811/1‑150 (MQ=255)
                                                                                   tctcGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATCCTACCGGGCAGGGGAAACCCCCGCGACCAGATACA‑ACCCTTCCCCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTTCTTTGCCGCCAGCAAATCTGCTATcc                                                             >  1:1062085/1‑151 (MQ=255)
                                                                                   tctcGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATAACGGTCAGGGGAATCCACCGCGACCAGATACA‑GCCCTTCCCCTTTTTCCGGTGCTGCCGCCAGCGGTCTTTCCTTTGCCGCCCGCAACTCTGCTATcc                                                             >  2:1510029/1‑151 (MQ=255)
                                                                                      cGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGGCAGGGGAATCCACCGCGACCCGATACA‑GCCCCTCCGCTTTTTCCGGTGCTGCCACCAGCGTTCTGTCCTTTTCCGCCAGCAAATCTGCTATCCAg                                                           >  1:1842755/1‑150 (MQ=255)
                                                                                      cGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCATCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAg                                                           <  1:309862/150‑1 (MQ=255)
                                                                                      cGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCa                                                            <  1:1720766/149‑1 (MQ=255)
                                                                                      cGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGGAATCCACCGCGACCAGATACA‑GCCCTTCCCCTTTTTCCGTTTCTGCCGCCCGCGTTTTGTCCTTTTCCGCCAGCAACCCTGCTATcc                                                             >  2:476094/1‑148 (MQ=255)
                                                                                       gTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGGAGATCATACCGGGCAGGGGAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTTCTGCCCCCAGCGTTCTGTCCTTTTCCGCCAGCAACTCTGCTTTCCCGc                                                          >  2:110566/1‑150 (MQ=255)
                                                                                         tAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATTATACCGGTCAGGGGAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGc                                                          >  2:534106/1‑148 (MQ=255)
                                                                                          aGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGctct                                                       <  2:1724623/150‑1 (MQ=255)
                                                                                          aGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGctct                                                       <  1:1906759/150‑1 (MQ=255)
                                                                                              cGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGGCAGGGGAAACCACCGCGACCAGATACA‑GCCCTTCCGCTTTTTCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCgg                                                   >  2:1554473/1‑150 (MQ=255)
                                                                                                ccAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTg                                                <  1:1470168/151‑1 (MQ=255)
                                                                                                 cAGAAATAGCGGGCCCATTGGCGGTTTTTGAAGATCATACCGGGCAGGGGAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCCGCGGTCTGTCCTTTTCCGCCAGCAACTCTTCTATCCAGCTCTCCGGCTgg                                               >  1:1366809/1‑151 (MQ=255)
                                                                                                 cAGAAATAGCGGGCCCATTGGCGGTTTTGGGAGGATATAACGGTGAAGGTAAATAACCCCGACCACACACACGCCCTTCC‑CTTTTGTCGGTGCTGCCCCCCGCGGTTTGTGCTTTGTCGCCAGCAAATCTTCCATCCAGCTCTCCgg                                                   >  2:1663764/1‑147 (MQ=255)
                                                                                                 cAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTgg                                               <  2:583189/151‑1 (MQ=255)
                                                                                                  aGAAATAGCGGGCCCATTGGCGGTTTTTGGAGATTATACCCGGGAGGGGAATCCACCCCCACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCCCCCGCGTTCTGTCCTTTTCCGCCCGCAACTCTGCTTTTCCGCTCCccggccgg                                               >  1:1359088/1‑150 (MQ=255)
                                                                                                   gAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGtt                                             <  1:445168/151‑1 (MQ=255)
                                                                                                      aTAGCGGGCCCATTGGCGGTTTTTGGAGATTATAACGGGCAGGGTAATCCACCGCGGCCCGATTCA‑GCCCTTTCGCTTTTTCCGTTGCTGCCCCCCCCGCTCTGTTCCTTTCCGCCAGCCACTCCGCTATCCAGCTCTCCGGCTGGgtgt                                           >  1:666806/1‑150 (MQ=255)
                                                                                                      aTAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCCGTCAGGGTAATCCACCGCGACCAGATACA‑ACCCCTCCGCTTTTTCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTtgtg                                          >  2:483879/1‑151 (MQ=255)
                                                                                                       tAGCGGGCCCATTGGCGGTTTTTGAAGATCATAACGGTCAGGGGAATCCACCGCGACCCGATACA‑GCCCTTCCGCTTTTTCCGTTGATGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCCGCTCTCCCGCTGGTTGTgg                                         >  1:1711802/1‑151 (MQ=255)
                                                                                                        aGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTggg                                        <  1:610722/151‑1 (MQ=255)
                                                                                                        aGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTgg                                         <  1:532399/150‑1 (MQ=255)
                                                                                                        aGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTgg                                         <  2:218169/150‑1 (MQ=255)
                                                                                                          cGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCa                                      <  2:1479914/151‑1 (MQ=255)
                                                                                                          cGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCa                                      <  2:1415707/151‑1 (MQ=255)
                                                                                                             gCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCg                                   <  2:220382/151‑1 (MQ=255)
                                                                                                             gCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCg                                   <  1:154320/151‑1 (MQ=255)
                                                                                                              cccATTGGCGGTTTTGGAAGATCATACCGGTCAGGGGAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCCGCTGGGTGTGGGCAcc                                    >  2:365365/1‑149 (MQ=255)
                                                                                                                   tGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTc                              <  1:1579293/150‑1 (MQ=255)
                                                                                                                   tGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTc                              <  2:668777/150‑1 (MQ=255)
                                                                                                                     gCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCAt                           <  1:1572397/151‑1 (MQ=255)
                                                                                                                      cGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATc                          >  1:546525/1‑151 (MQ=255)
                                                                                                                      cGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATc                          >  1:1796433/1‑151 (MQ=255)
                                                                                                                      cGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATc                          <  2:1023826/151‑1 (MQ=255)
                                                                                                                       ggTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCTGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATc                          <  2:386063/150‑1 (MQ=255)
                                                                                                                            tGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCt                     <  1:897983/150‑1 (MQ=255)
                                                                                                                             ggAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGc                   <  1:1789922/151‑1 (MQ=255)
                                                                                                                              gAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTgtc                  <  1:239712/151‑3 (MQ=255)
                                                                                                                              gAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGcc                  <  2:1089401/151‑1 (MQ=255)
                                                                                                                                    cATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACaa             <  2:1632047/150‑1 (MQ=255)
                                                                                                                                    cATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAat            >  1:32815/1‑151 (MQ=255)
                                                                                                                                    cATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTCCCATCAGGCTGCCGACaa             <  1:1527368/150‑1 (MQ=255)
                                                                                                                                     aTACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAata           <  2:259768/151‑1 (MQ=255)
                                                                                                                                     aTACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAat            >  2:93168/1‑150 (MQ=255)
                                                                                                                                        ccGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTc        >  1:632781/1‑151 (MQ=255)
                                                                                                                                             cAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGAc   >  2:1086230/1‑151 (MQ=255)
                                                                                                                                              aGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGAcc  >  2:896414/1‑151 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
GTCCATATTTGCCTATGCCTTGTACTCGTCATCTTTCAGGCTGTAACTACGTTGGCTACAGCCTGAAATGTTCCGAGCATTATTCTCGTTAGTCCGCCAGAAATAGCGGGCCCATTGGCGGTTTTGGAAGATCATACCGGTCAGGGTAATCCACCGCGACCAGATACA‑GCCCTTCCGCTTTTGCCGTTGCTGCCGCCAGCGTTCTGTCCTTTGCCGCCAGCAACTCTGCTATCCAGCTCTCCGGCTGGTTGTGGGCACCGACTTCCATCAGGCTGCCGACAATATTCCTGACC  >  NC_000913/2434736‑2435028

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑