Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,356,201 | 0 | T | G | 46.4% | 84.0 / 12.8 | 56 | T304P (ACC→CCC) | sapA | antimicrobial peptide transport ABC transporter periplasmic binding protein |
Reads supporting (aligned to +/- strand): ref base T (8/22); new base G (26/0); total (34/22) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 3.66e-09 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGCGGTGTTAAATGCCAGATAGGCGAC‑GTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCA‑GGCCAGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGC > NC_000913/1356054‑1356350 | cGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGCGGTGtt < 2:576864/151‑1 (MQ=255) cATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGCTTTGGGGGGGTGaaa > 1:67518/1‑149 (MQ=255) aTAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGTCGTACCATAATAGGGGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTCCGAGCGCATGGCGGACAGCGGGATCATTTAGCGGCGG‑CTTGGCGGGGGTaaagat > 1:82277/1‑147 (MQ=255) aTAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGCGGTGTTAAATGcc < 1:186073/151‑1 (MQ=255) cccACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑GTTGGCGGGGGTAAATGcc > 1:695999/1‑146 (MQ=255) ccACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGCGGTGTTAAATGCCAGATAg < 1:483177/151‑1 (MQ=255) cACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGCGGTGTTAAATGCCAGATAgg < 2:490492/151‑1 (MQ=255) aCGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGCGGTGTTAAATGCCAGATAGGc < 2:469733/151‑1 (MQ=255) aCGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGCGGTGTTAAATGCCAGATAGGc < 1:26427/151‑1 (MQ=255) cGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTTGCGGGGGTAAATGCCAGAAAAGCg > 2:876830/1‑151 (MQ=255) gAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTTGCGGGGGTAAAAGCCAGATAGGCg > 2:970743/1‑150 (MQ=255) cgGTAAAATAGAGGCCGCCGTTTCAGCCGTACCAT‑‑TAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGCGGTGTTAAATGCCAGATAGGCGAC‑GTTCATccc < 1:1012431/151‑1 (MQ=255) ggTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTTGCGGGGGTAAATGCCAGATAAGCGAC‑GGTCATcc > 2:1017486/1‑151 (MQ=255) aaTAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGCGGGGGTTAAAGGCAGAAAGGCGACGGTTCTTcccgg > 1:833107/1‑148 (MQ=255) gccgTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGCGGGGGTAAATGCCAGATAAGCGGC‑GGTCATCCCCGGGCGCAGCGtt > 2:104406/1‑150 (MQ=255) ccgTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGCGGTGTTAAATGCCAGATAGGCGAC‑GTTCATCCCAGGACGCAGCGTTaa < 1:557167/151‑1 (MQ=255) tttCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGCGGGGTTAAATGACAGAAAAGCGAC‑GTTCATCCCCGGGCGCAGGGTGaaa > 1:198882/1‑149 (MQ=255) tCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGCGGGGGTAAATGCCAGAAAGGCGAC‑GGTCATCCCAAGACGCAGCGTTaaa > 2:110997/1‑147 (MQ=255) gTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGCGGGGTTAAATGCCAGATAGGCGAC‑GTTCATCCCAGGACGCAGCGTTaaa > 2:508073/1‑141 (MQ=255) ccATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGCGGGGGTTAATTCCAGAAAGGCGAC‑GTTTATCCCCGGAAGCAGCGCTAAACGCAAACGCggg > 1:564275/1‑150 (MQ=255) aataGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGCGGTGTTAAATGCCAGATAGGCGAC‑GTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGgtcgtc < 1:861904/151‑1 (MQ=255) aataGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGCGGGGGTAAAAGCCAGAAAGGCGAC‑GTTTATCCCCGGACGCAGCGTTAAAACCAAGCGCGGggcgtc > 1:833415/1‑151 (MQ=255) gATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGCGGTGTTAAATGCCAGATAGGCGAC‑GTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGc < 2:163889/151‑1 (MQ=255) gCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGCGGTGTTAAATGCCAGATAGGCGAC‑GTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGa < 2:1051387/151‑1 (MQ=255) cATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGCGGGGTTAAATGCCAGAAAGGCGAC‑GTTCATCCCAGGGCGCAGCGTTAAACGCAAGCGCGGGGCGTCACGCAAAAAgg > 2:503128/1‑149 (MQ=255) tCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGCGGGGTTTAAAGCCCGATAGGCGGC‑GTTCATCCCCGGACGCAGCGTTAAAAGCAAGCGCGGGGCGGCACGCaaaa > 1:234081/1‑144 (MQ=255) cAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGCGGTGTTAAATGCCAGATAGGCGAC‑GTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAgc < 2:124860/151‑1 (MQ=255) ggCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGCGGTGTTAAATGCCAGATAGGCGAC‑GTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAgctg < 2:134490/151‑1 (MQ=255) cgcTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGCGGTGTTAAATGCCAGATAGGCGAC‑GTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAgctggc < 1:259102/151‑1 (MQ=255) cTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGCGGGGTTAAATTCCAGATAGGCGAC‑GTTCATCCCCGGGCGCAGCGTTTAACGCAAGCGCGGGGCGTCACGCAAAATGGGTAgctg > 2:307291/1‑147 (MQ=255) ggTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTTGCGGTGTTTAAAGCCAGATAGGGGGC‑GGTCATCCCACGACGCAGCGGTAAACCCAAACGGGGGGCGGCACGCAAAAAGGATAGctggctgg > 1:405688/1‑150 (MQ=255) attaATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGCGGTGTTAAATGCCAGATAGGCGAC‑GTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGgcagca < 2:163775/151‑1 (MQ=255) taATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGCGGTGTTAAATGCCAGATAGGCGAC‑GTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGcagca‑g < 1:551098/150‑1 (MQ=255) aaTCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGCGGTGTTTAAAGCCACATACGCGAC‑GTTCATCCCCGGGCGCCGCGGTAAACGCAAGCGCGGGGCGGCACCCAAAAAGGATAGGTGGCTGgcag > 1:1041763/1‑146 (MQ=255) aaTCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGCGGTGTTAAATGCCAGATAGGCGAC‑GTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGca‑ggc < 2:747088/151‑1 (MQ=255) aTCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGCGGGGTTTAATGCCAGATAAGCGAC‑GGTCATCCCAGGACGCAGCGTTAAAAGCAAGCGCGGGGCGGCACGCAAAAAGGATAActtgctgg > 2:432695/1‑142 (MQ=255) tCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTTGGGGGGTTAAAAGCCCGATAAGCGAC‑GTTCATCCCAGGACGCAGCGTTAAAAGCAAACGCGGGGCGTCACGCAAAATGGATAGCTGGCTGGGAGca‑ggcc > 2:360112/1‑150 (MQ=255) gCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTTGCGGGGGTAAATGCCCGATAAGCGAC‑GTTTATCCCCGGGCGCCGCGGTTAACGCAAAAGCGGGGCGTCACGCAAAATGGATAGCCGGCCTGCAGcc‑ggccc > 1:720537/1‑148 (MQ=255) gCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGCGGGGGTAAAAGCCAGAAAAGCCAC‑GGTCATCCCCGGACGCAGCGGTAAAAGCAAGCGCGGGGCGGCACGCAAAAAGGGTAaatggctg > 1:372282/1‑138 (MQ=255) ccAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTTGCGGGGTTAAATGCCAGATAGGCGAC‑GTTCATCCCCGGACGCCGCGGTAAAAGCAAGCGCGGGTCGGCACGCAAAATGGATAGCTGGCTGGGAGca‑ggcccgg > 2:457453/1‑150 (MQ=255) ccAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGCGGGGTTAAAAGCCAGATAGGCGAC‑GTTCATCCCCGGACGCAGCGTTAAAAGCAAACGCGGGGCGGCACGCAAAATGGATAGCTGGCTGGCAGca‑ggcccgg > 2:277657/1‑150 (MQ=255) tGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTTGCGGTGGTAAATGCCAGAGAAGGGAC‑GGTCATCCCCAGACGCAGCGGTAAACGCAAGCGCGGGGCGGCCCGCAAAATAGATAGATGGCTGGCAGCagggccaggc > 1:638895/1‑148 (MQ=255) cgcATGGCGGACAGCGGGATTATTTAGCGGCGG‑ATTTGCGGGGTTAAAAGCCACAAAGGCGAC‑GTTCATCCCAAGACGCAGCGGTAAACGCAAGCGCCGGGCGTCACGCCAAAAGGGTAACTGGCTGGCCGCA‑GGCCCCGCCAGaa > 1:885998/1‑146 (MQ=255) cATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTTGCGGTGTTAAATGCCAGATAAGCGGC‑GTTCATCCCCGGACGCAGCGTTTAAAGCAAGCGCGGGGCGGCCCGCAAAATAGATAGCTGGCTGGCAGCC‑GGCCCGGCCCGaa > 1:343952/1‑144 (MQ=255) ggCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGCGGTGTTAAATGCCAGATAGGCGAC‑GTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCA‑GGCCAGGCCAGAACGTCGCAtt < 1:56795/150‑1 (MQ=255) gACAGCGGGATTATTTAGCGGCGG‑TTTGGCGGGGTTAAATGCCACATAGGCGAC‑GTTCATCCCAGGACGCAGCGTTAAACGCAAACGCGGGGCGTCACGCCAAATGGATAGGTGGCTGGCAGCA‑GGCCCGGCCAGAACGTCGCATTcccc > 2:569136/1‑150 (MQ=255) aCAGCGGGATTATTTAGCGGCGG‑TTTTGCGGGGGTAAATGCCACAGAAGTGGC‑GTTTATCCCCAGAACCCGCGTTGAAAACCAGAGCGGGTGGTCACGCAAAAAGGGTAGCCAGCTGGCCACC‑AGCCACGCCCGAACGGCCCATAcccccg > 2:506953/1‑149 (MQ=255) cGGGATTATTTAGCGGCGG‑TTTGGCGGGGGTAAATTCCAGATAGGGGAC‑GGTTATTCCAGGACGCAGCGTTAAACGCAAACGCGGGTCGTCACGCAAAAAGGATAGCTCGCTGGCAGCC‑AGGCAGGGCAGAACGTCGCCTTcccccg > 1:744749/1‑145 (MQ=255) tattTAGCGGCGG‑TTTGGCGGTGTTAAATGCCAGATAGGCGAC‑GTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCA‑GGCCAGGCCAGAACGTCGCATTCCCCGGTCAGGAGtt < 2:619097/150‑1 (MQ=255) attTAGCGGCGG‑TTTGGCGGTGTTAAATGCCAGATAGGCGAC‑GTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCA‑GGCCAGGCCAGAACGTCGCATTCCCCGGTCAGGAGttt < 1:979286/150‑1 (MQ=255) attTAGCGGCGG‑TTTGGCGGTGTTAAATGCCAGATAGGCGAC‑GTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCA‑GGCCAGGCCAGAACGTCGCATTCCCCGGTCAGGAGttt < 2:378758/150‑1 (MQ=255) gcggcgG‑TTTGGCGGTGTTAAATGCCAGATAGGCGAC‑GTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCA‑GGCCAGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTc > 2:125731/1‑146 (MQ=255) gg‑TTTGGCGGTGTTAAATGCCAGATAGGCGAC‑GTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCA‑GGCCAGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTc > 2:46970/1‑151 (MQ=255) tGGCGGTGTTAAATGCCAGATAGGCGAC‑GTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCA‑GGCCAGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCgg > 1:1041271/1‑150 (MQ=255) gCGGTGTTAAATGCCAGATAGGCGAC‑GTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCA‑GGCCAGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCg < 1:523674/147‑1 (MQ=255) ggTGTTAAATGCCAGATAGGCGAC‑GTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCA‑GGCCAGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTgcc > 1:215186/1‑150 (MQ=255) gtgtTAAATGCCAGATAGGCGAC‑GTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCA‑GGCCAGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTgccgc > 2:227151/1‑151 (MQ=255) | CGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGG‑TTTGGCGGTGTTAAATGCCAGATAGGCGAC‑GTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCA‑GGCCAGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGC > NC_000913/1356054‑1356350 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |