Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,356,2010TG46.4% 84.0 / 12.8 56T304P (ACC→CCC) sapAantimicrobial peptide transport ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base T (8/22);  new base G (26/0);  total (34/22)
Fisher's exact test for biased strand distribution p-value = 3.66e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGCGGTGTTAAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCAGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGC  >  NC_000913/1356054‑1356350
                                                                                                                                                    |                                                                                                                                                       
cGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGCGGTGtt                                                                                                                                                      <  2:576864/151‑1 (MQ=255)
      cATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGCTTTGGGGGGGTGaaa                                                                                                                                                   >  1:67518/1‑149 (MQ=255)
       aTAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGTCGTACCATAATAGGGGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTCCGAGCGCATGGCGGACAGCGGGATCATTTAGCGGCGGCTTGGCGGGGGTaaagat                                                                                                                                                >  1:82277/1‑147 (MQ=255)
       aTAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGCGGTGTTAAATGcc                                                                                                                                               <  1:186073/151‑1 (MQ=255)
            cccACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGGTTGGCGGGGGTAAATGcc                                                                                                                                               >  1:695999/1‑146 (MQ=255)
             ccACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGCGGTGTTAAATGCCAGATAg                                                                                                                                         <  1:483177/151‑1 (MQ=255)
              cACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGCGGTGTTAAATGCCAGATAgg                                                                                                                                        <  2:490492/151‑1 (MQ=255)
               aCGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGCGGTGTTAAATGCCAGATAGGc                                                                                                                                       <  2:469733/151‑1 (MQ=255)
               aCGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGCGGTGTTAAATGCCAGATAGGc                                                                                                                                       <  1:26427/151‑1 (MQ=255)
                cGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTTGCGGGGGTAAATGCCAGAAAAGCg                                                                                                                                      >  2:876830/1‑151 (MQ=255)
                 gAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTTGCGGGGGTAAAAGCCAGATAGGCg                                                                                                                                      >  2:970743/1‑150 (MQ=255)
                         cgGTAAAATAGAGGCCGCCGTTTCAGCCGTACCAT‑‑TAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGCGGTGTTAAATGCCAGATAGGCGACGTTCATccc                                                                                                                          <  1:1012431/151‑1 (MQ=255)
                          ggTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTTGCGGGGGTAAATGCCAGATAAGCGACGGTCATcc                                                                                                                           >  2:1017486/1‑151 (MQ=255)
                               aaTAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGCGGGGGTTAAAGGCAGAAAGGCGACGGTTCTTcccgg                                                                                                                        >  1:833107/1‑148 (MQ=255)
                                         gccgTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGCGGGGGTAAATGCCAGATAAGCGGCGGTCATCCCCGGGCGCAGCGtt                                                                                                             >  2:104406/1‑150 (MQ=255)
                                          ccgTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGCGGTGTTAAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTaa                                                                                                           <  1:557167/151‑1 (MQ=255)
                                             tttCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGCGGGGTTAAATGACAGAAAAGCGACGTTCATCCCCGGGCGCAGGGTGaaa                                                                                                          >  1:198882/1‑149 (MQ=255)
                                               tCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGCGGGGGTAAATGCCAGAAAGGCGACGGTCATCCCAAGACGCAGCGTTaaa                                                                                                          >  2:110997/1‑147 (MQ=255)
                                                     gTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGCGGGGTTAAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTaaa                                                                                                          >  2:508073/1‑141 (MQ=255)
                                                        ccATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGCGGGGGTTAATTCCAGAAAGGCGACGTTTATCCCCGGAAGCAGCGCTAAACGCAAACGCggg                                                                                              >  1:564275/1‑150 (MQ=255)
                                                            aataGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGCGGTGTTAAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGgtcgtc                                                                                         <  1:861904/151‑1 (MQ=255)
                                                            aataGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGCGGGGGTAAAAGCCAGAAAGGCGACGTTTATCCCCGGACGCAGCGTTAAAACCAAGCGCGGggcgtc                                                                                         >  1:833415/1‑151 (MQ=255)
                                                                gATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGCGGTGTTAAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGc                                                                                     <  2:163889/151‑1 (MQ=255)
                                                                        gCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGCGGTGTTAAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGa                                                                             <  2:1051387/151‑1 (MQ=255)
                                                                         cATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGCGGGGTTAAATGCCAGAAAGGCGACGTTCATCCCAGGGCGCAGCGTTAAACGCAAGCGCGGGGCGTCACGCAAAAAgg                                                                              >  2:503128/1‑149 (MQ=255)
                                                                           tCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGCGGGGTTTAAAGCCCGATAGGCGGCGTTCATCCCCGGACGCAGCGTTAAAAGCAAGCGCGGGGCGGCACGCaaaa                                                                                 >  1:234081/1‑144 (MQ=255)
                                                                            cAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGCGGTGTTAAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAgc                                                                         <  2:124860/151‑1 (MQ=255)
                                                                              ggCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGCGGTGTTAAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAgctg                                                                       <  2:134490/151‑1 (MQ=255)
                                                                                cgcTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGCGGTGTTAAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAgctggc                                                                     <  1:259102/151‑1 (MQ=255)
                                                                                  cTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGCGGGGTTAAATTCCAGATAGGCGACGTTCATCCCCGGGCGCAGCGTTTAACGCAAGCGCGGGGCGTCACGCAAAATGGGTAgctg                                                                       >  2:307291/1‑147 (MQ=255)
                                                                                    ggTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTTGCGGTGTTTAAAGCCAGATAGGGGGCGGTCATCCCACGACGCAGCGGTAAACCCAAACGGGGGGCGGCACGCAAAAAGGATAGctggctgg                                                                  >  1:405688/1‑150 (MQ=255)
                                                                                        attaATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGCGGTGTTAAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGgcagca                                                             <  2:163775/151‑1 (MQ=255)
                                                                                          taATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGCGGTGTTAAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGcagcag                                                           <  1:551098/150‑1 (MQ=255)
                                                                                           aaTCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGCGGTGTTTAAAGCCACATACGCGACGTTCATCCCCGGGCGCCGCGGTAAACGCAAGCGCGGGGCGGCACCCAAAAAGGATAGGTGGCTGgcag                                                               >  1:1041763/1‑146 (MQ=255)
                                                                                           aaTCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGCGGTGTTAAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGcaggc                                                         <  2:747088/151‑1 (MQ=255)
                                                                                            aTCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGCGGGGTTTAATGCCAGATAAGCGACGGTCATCCCAGGACGCAGCGTTAAAAGCAAGCGCGGGGCGGCACGCAAAAAGGATAActtgctgg                                                                  >  2:432695/1‑142 (MQ=255)
                                                                                             tCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTTGGGGGGTTAAAAGCCCGATAAGCGACGTTCATCCCAGGACGCAGCGTTAAAAGCAAACGCGGGGCGTCACGCAAAATGGATAGCTGGCTGGGAGcaggcc                                                        >  2:360112/1‑150 (MQ=255)
                                                                                               gCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTTGCGGGGGTAAATGCCCGATAAGCGACGTTTATCCCCGGGCGCCGCGGTTAACGCAAAAGCGGGGCGTCACGCAAAATGGATAGCCGGCCTGCAGccggccc                                                       >  1:720537/1‑148 (MQ=255)
                                                                                               gCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGCGGGGGTAAAAGCCAGAAAAGCCACGGTCATCCCCGGACGCAGCGGTAAAAGCAAGCGCGGGGCGGCACGCAAAAAGGGTAaatggctg                                                                   >  1:372282/1‑138 (MQ=255)
                                                                                                ccAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTTGCGGGGTTAAATGCCAGATAGGCGACGTTCATCCCCGGACGCCGCGGTAAAAGCAAGCGCGGGTCGGCACGCAAAATGGATAGCTGGCTGGGAGcaggcccgg                                                     >  2:457453/1‑150 (MQ=255)
                                                                                                ccAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGCGGGGTTAAAAGCCAGATAGGCGACGTTCATCCCCGGACGCAGCGTTAAAAGCAAACGCGGGGCGGCACGCAAAATGGATAGCTGGCTGGCAGcaggcccgg                                                     >  2:277657/1‑150 (MQ=255)
                                                                                                    tGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTTGCGGTGGTAAATGCCAGAGAAGGGACGGTCATCCCCAGACGCAGCGGTAAACGCAAGCGCGGGGCGGCCCGCAAAATAGATAGATGGCTGGCAGCagggccaggc                                                    >  1:638895/1‑148 (MQ=255)
                                                                                                          cgcATGGCGGACAGCGGGATTATTTAGCGGCGGATTTGCGGGGTTAAAAGCCACAAAGGCGACGTTCATCCCAAGACGCAGCGGTAAACGCAAGCGCCGGGCGTCACGCCAAAAGGGTAACTGGCTGGCCGCAGGCCCCGCCAGaa                                               >  1:885998/1‑146 (MQ=255)
                                                                                                            cATGGCGGACAGCGGGATTATTTAGCGGCGGTTTTGCGGTGTTAAATGCCAGATAAGCGGCGTTCATCCCCGGACGCAGCGTTTAAAGCAAGCGCGGGGCGGCCCGCAAAATAGATAGCTGGCTGGCAGCCGGCCCGGCCCGaa                                               >  1:343952/1‑144 (MQ=255)
                                                                                                               ggCGGACAGCGGGATTATTTAGCGGCGGTTTGGCGGTGTTAAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCAGGCCAGAACGTCGCAtt                                      <  1:56795/150‑1 (MQ=255)
                                                                                                                   gACAGCGGGATTATTTAGCGGCGGTTTGGCGGGGTTAAATGCCACATAGGCGACGTTCATCCCAGGACGCAGCGTTAAACGCAAACGCGGGGCGTCACGCCAAATGGATAGGTGGCTGGCAGCAGGCCCGGCCAGAACGTCGCATTcccc                                  >  2:569136/1‑150 (MQ=255)
                                                                                                                    aCAGCGGGATTATTTAGCGGCGGTTTTGCGGGGGTAAATGCCACAGAAGTGGCGTTTATCCCCAGAACCCGCGTTGAAAACCAGAGCGGGTGGTCACGCAAAAAGGGTAGCCAGCTGGCCACCAGCCACGCCCGAACGGCCCATAcccccg                                >  2:506953/1‑149 (MQ=255)
                                                                                                                        cGGGATTATTTAGCGGCGGTTTGGCGGGGGTAAATTCCAGATAGGGGACGGTTATTCCAGGACGCAGCGTTAAACGCAAACGCGGGTCGTCACGCAAAAAGGATAGCTCGCTGGCAGCCAGGCAGGGCAGAACGTCGCCTTcccccg                                >  1:744749/1‑145 (MQ=255)
                                                                                                                              tattTAGCGGCGGTTTGGCGGTGTTAAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCAGGCCAGAACGTCGCATTCCCCGGTCAGGAGtt                       <  2:619097/150‑1 (MQ=255)
                                                                                                                               attTAGCGGCGGTTTGGCGGTGTTAAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCAGGCCAGAACGTCGCATTCCCCGGTCAGGAGttt                      <  1:979286/150‑1 (MQ=255)
                                                                                                                               attTAGCGGCGGTTTGGCGGTGTTAAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCAGGCCAGAACGTCGCATTCCCCGGTCAGGAGttt                      <  2:378758/150‑1 (MQ=255)
                                                                                                                                    gcggcgGTTTGGCGGTGTTAAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCAGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTc                     >  2:125731/1‑146 (MQ=255)
                                                                                                                                         ggTTTGGCGGTGTTAAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCAGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTc           >  2:46970/1‑151 (MQ=255)
                                                                                                                                              tGGCGGTGTTAAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCAGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCgg        >  1:1041271/1‑150 (MQ=255)
                                                                                                                                                gCGGTGTTAAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCAGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCg         <  1:523674/147‑1 (MQ=255)
                                                                                                                                                  ggTGTTAAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCAGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTgcc    >  1:215186/1‑150 (MQ=255)
                                                                                                                                                   gtgtTAAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCAGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTgccgc  >  2:227151/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                       
CGTTGTCATAGGCCCACGAGGCGCGCGGTAAAATAGAGGCCGCCGTTTCAGCCGTACCATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGTTTGGCGGTGTTAAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTGGCAGCAGGCCAGGCCAGAACGTCGCATTCCCCGGTCAGGAGTTTCGACAGACGTCCGGTGCCGC  >  NC_000913/1356054‑1356350

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: