Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,825,0610GA42.2% 96.2 / 35.0 71intergenic (‑124/+79)ves/spycold‑ and stress‑inducible protein/periplasmic ATP‑independent protein refolding chaperone, stress‑induced
Reads supporting (aligned to +/- strand):  ref base G (34/7);  new base A (0/30);  total (34/37)
Fisher's exact test for biased strand distribution p-value = 2.15e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATT  >  NC_000913/1824913‑1825211
                                                                                                                                                        |                                                                                                                                                      
ttttACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGagaaaaga                                                                                                                                                       >  2:1091357/1‑150 (MQ=255)
  ttACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGagaaaagaaa                                                                                                                                                     <  1:1002348/150‑1 (MQ=255)
      gCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAAccgc                                                                                                                                                <  2:610122/151‑1 (MQ=255)
        atatCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAAccgcc                                                                                                                                               <  1:729584/150‑1 (MQ=255)
             aaaGTATTCCATGCTCGCTCCCGGCCTGTTCTTTTCATTTTTATCGTTCAGGTAACCGTGGATGTTTGTCGGAAACTCGGGTGATTTACTTTAGTGGTTATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGa                                                                                                                                             <  1:364436/147‑1 (MQ=255)
                gTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTc                                                                                                                                      >  1:743181/1‑151 (MQ=255)
                    tCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCAcc                                                                                                                                  >  1:735323/1‑151 (MQ=255)
                       atcctcccccccGCCCTGTTCTTTTTATTTTTTTCTTTCAGGTACCGGGGGATGTTTATCGCCACCTCGGGTGTTTTACGTTAGTGGTGACCAGTTTTTTTTTCCAATTTTTAGAAAAAAGAAAAAAAAACCGCCGATCCTGTCCACCGCa                                                                                                                               <  2:97498/142‑1 (MQ=255)
                       aTACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGc                                                                                                                                >  2:982536/1‑150 (MQ=255)
                            cGCTCCCGGCCCTTTTTTTTCTTTTTTATCGTTCAGGTAACGGTGGTTGTTTATCGGCAACTGGGGTGATTTACGTTAGTGGTGATCAGGTTTTTTTTACAACTGTTTGAGAAGAGAAAAAAAAACCGCCGATCCTGTCCACCGCATTACt                                                                                                                          <  2:1007020/151‑1 (MQ=255)
                            cGCCCCCGGCCCGTTCTTTTCTTTGTTTTCGTTAAGGTAACGGTGGTTGTTTAGCGGCAACTGGGGTATTTTACGTTAGGGGTGATCAGTTTTTTTTTACAACTTTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACt                                                                                                                          <  1:67903/151‑1 (MQ=255)
                              cccccGGCCCTTTCTTTTTATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCACCGGGGTTGTTTTACTTTAGGGGTGATCAGTTTTTTTTCACAATTGTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTAc                                                                                                                           <  1:457274/146‑1 (MQ=255)
                                  cGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAgg                                                                                                                    >  2:764633/1‑151 (MQ=255)
                                   ggCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGt                                                                                                                   >  1:148750/1‑151 (MQ=255)
                                     cccGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGt                                                                                                                   >  1:891854/1‑149 (MQ=255)
                                     cccGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGt                                                                                                                   >  2:822285/1‑149 (MQ=255)
                                           cTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGAc                                                                                                            >  1:1059829/1‑150 (MQ=255)
                                            ttttCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACa                                                                                                           >  1:628984/1‑150 (MQ=255)
                                            ttctCATTGTTGTCTTGCAGGTAACGGTGGGTTTTTCGCGACAGGTGGGGTTTTTTGTTTTAGGGGTGCCAAGGTTTTTTTCCCAGCTTTTTGAGAGAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACa                                                                                                           <  2:395872/147‑1 (MQ=255)
                                             tttCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACa                                                                                                           >  2:963313/1‑149 (MQ=255)
                                             tttAATTGTTATCGTTCAGGTAACGGGGGATGGTTATCGGCAACTGGGGTGATTTACGTTAGTGGTGATCAGGTTTTTTTTAAAACTGTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACaa                                                                                                          <  2:959090/150‑1 (MQ=255)
                                              tttaTTTTTATCGTTCAGGTAACCGGGTTTGTTTATCGGCAACTCGGGTGATTTACTTTAGTGGTTATCAGGTTTTTTTTACAATTGTTTGAAAAGAAAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAg                                                                                                         <  1:183498/147‑1 (MQ=255)
                                              ttCATTGTTATCGTTCAGGTACCCGTGGATGTTTACGGGCACCTGGGGTGTTTTACGTTAGTGGTGATCGGGTTTTTTTTACAACTTTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAg                                                                                                         <  2:465951/150‑1 (MQ=255)
                                                 aTTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcg                                                                                                     >  2:504811/1‑151 (MQ=255)
                                                  ttGTTATCTTTAAGGTACCCGTGGTTGGTTATCGGCACCTCGGGTGTTTTACTTTAGTGGTGACCAGTTTTTTTTTACAACTTTTTGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGAcc                                                                                                      <  1:580576/149‑1 (MQ=255)
                                                   tGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcggc                                                                                                   >  1:120302/1‑151 (MQ=255)
                                                     ttATCGTTCAGGTAACCGGGGAGGTTTATCGGCAACTGGGTTGTTTTACGTTGGTGGTGATCAGTTTTTTTTTACACTTGTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACcggcg                                                                                                  <  2:138494/150‑1 (MQ=255)
                                                         tcgtccatgaaCCGGGGATTGTTTATCGGCAACTGGGGTGATTTAGGTAGGTGGTGTTCAGTTTTTTTTAAAAATGTTTTAAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTc                                                                                               <  1:958779/141‑1 (MQ=255)
                                                          gTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCtt                                                                                             >  2:602820/1‑150 (MQ=255)
                                                           ttCAGGTACCGGTGGATGTTTACCGGCAACTCGGGTGATTTACGTTGGTGGTGATCGGGTTTTTTTTACAACTGTTGGAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTa                                                                                            <  2:259538/150‑1 (MQ=255)
                                                            tccggTAACCCGGGGTTGTTTATCGCAAACTGGGGTGTTTTCGTTTATTGGTGATCAGGTTTTTTTTACACCTGTTTAAAAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGt                                                                                                <  2:37468/143‑1 (MQ=255)
                                                               ggTACCGGTGGATGGTTATCGGCAACTGGGGTGTTTTACGTTAGTGGTGATCAGGTTTTTTTTACAACTTTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTaa                                                                                           <  2:774335/147‑1 (MQ=255)
                                                               ggTACCCGGGGATGGTTATGGGCAACTGGGGTTATTTACTTTAGTGGTAATCAGGTTTTTTTTACAATTGTTTGAAAAAAGAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTaa                                                                                           <  2:808267/147‑1 (MQ=255)
                                                                   aCCGTGGATGGTTATCGGCAACTCGGGAGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGtttttt                                                                                    >  1:872230/1‑150 (MQ=255)
                                                                     ccGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTgg                                                                                  >  2:574503/1‑151 (MQ=255)
                                                                       gTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTgg                                                                                  >  1:1062795/1‑149 (MQ=255)
                                                                       gTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGc                                                                                 >  2:1014378/1‑150 (MQ=255)
                                                                         ggATGGTTCTCGGCAACTCGGGTGATTTACTTTAGTGTTGACCAGGTTTTTTTTACAACTTTTGGAAAAGAGAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGc                                                                                 <  1:54087/148‑1 (MQ=255)
                                                                             tGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTTGCTg                                                                               >  1:79795/1‑147 (MQ=255)
                                                                                ttATCGGCATCGGGGGTAATTTACTTTGGTGGTGATCAGTTTTTTTTTACAACTGTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGa                                                                          <  1:833634/149‑1 (MQ=255)
                                                                                  aTCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTTGCTGAAAGatt                                                                        >  1:871150/1‑149 (MQ=255)
                                                                                     ggCACCTGGGGTGATTTCCGTTAGTGGTAATCAGGTTTTTTTTACAACTGTTTGAGAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTca                                                                   <  2:714118/151‑1 (MQ=255)
                                                                                     gaaatcaggggTGTTTTACGTTGGTGGTGAGAAGGTTTTTTTTACAGCTGTTGGAGAAAAAAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTc                                                                    <  2:743300/142‑1 (MQ=255)
                                                                                        aaCTGGGGTATTTTACGTTGGTGGTTATCGGTTTTTTTTTACACTTGTTGGAAAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTcagca                                                                <  1:1114228/151‑1 (MQ=255)
                                                                                             gggTGATTTACTTTAGTGTTGATCAGGTTTTTTTTACAACTGTTTGAAAAGAGAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTg                                                            <  2:926357/150‑1 (MQ=255)
                                                                                                tgttttACGTTAGTGGTGATCAGGTTTTTTTTACACCTTTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAg                                                         <  2:843712/147‑1 (MQ=255)
                                                                                                  aTTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAgg                                                        >  2:838873/1‑149 (MQ=255)
                                                                                                              ggtgATCAGGCTTTTTTTACAACTGTTTGAGAAGAAAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCttt                                            <  2:53947/149‑1 (MQ=255)
                                                                                                               ggtaaacggttttttttAAAATCTTTTTAAGAAGAGAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTa                                                 <  2:204119/134‑1 (MQ=255)
                                                                                                                   tCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGcc                                        >  1:675918/1‑148 (MQ=255)
                                                                                                                   tCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCt                                     >  2:141608/1‑151 (MQ=255)
                                                                                                                      ggCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTgg                                   >  2:983383/1‑150 (MQ=255)
                                                                                                                      ggCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTgg                                   >  2:413662/1‑150 (MQ=255)
                                                                                                                      ggCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTg                                    >  2:542640/1‑149 (MQ=255)
                                                                                                                         tttttttACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACg                                >  2:191851/1‑150 (MQ=255)
                                                                                                                             tttACAATTTTTTGAGAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCt                            <  2:287817/150‑1 (MQ=255)
                                                                                                                              ttaacatttGTTTAAGAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCt                            <  1:1080629/142‑1 (MQ=255)
                                                                                                                              ttACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTTAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGt                          >  1:865419/1‑151 (MQ=255)
                                                                                                                              taaCAACTGTTGGAAAAAAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTg                           <  2:630964/148‑1 (MQ=255)
                                                                                                                                aCAACTGTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTc                         <  2:865536/150‑1 (MQ=255)
                                                                                                                                  aaCTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGt                          >  1:290269/1‑147 (MQ=255)
                                                                                                                                  aaCTGTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCa                        <  1:658748/149‑1 (MQ=255)
                                                                                                                                   aaatttgtgaaaaagaaaaAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTc                         <  1:577773/137‑1 (MQ=255)
                                                                                                                                   aCTTTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGAc                     <  2:130123/151‑1 (MQ=255)
                                                                                                                                   aCTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGAc                     >  2:831453/1‑151 (MQ=255)
                                                                                                                                   aCTGTTTGAGAAGAAAAAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGa                      <  2:82751/150‑1 (MQ=255)
                                                                                                                                     cTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTTAGTTTTTTGGGTGGAAGATTATTCAGCAGGTGCAGGCATTTTACCTTTTGCCCCTGGAAGTTCTGTCAGAc                     >  2:94366/1‑150 (MQ=255)
                                                                                                                                      tGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTTAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGAAGTTCTGTCAGACGc                   >  1:23283/1‑151 (MQ=255)
                                                                                                                                          ttGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCt                  >  1:888411/1‑149 (MQ=255)
                                                                                                                                           tggaaagaaaaAAGAAACCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCATTTGCCGCTGGACGTTCTGTCAGACGCtt                 <  1:842093/145‑1 (MQ=255)
                                                                                                                                             agaagagaaAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCCTAAGGTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCCTTTGCCGCTGGACGTTCTGTCAGACGCTtctc              >  1:1082281/1‑150 (MQ=255)
                                                                                                                                               aagagaaAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCaa            >  2:757105/1‑150 (MQ=255)
                                                                                                                                               aagaaaaAAAAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTtctc              <  2:730198/148‑1 (MQ=255)
                                                                                                                                                      aagaaaaCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGc     >  2:442114/1‑150 (MQ=255)
                                                                                                                                                        gaaaaCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCAtt  >  2:475636/1‑151 (MQ=255)
                                                                                                                                                        |                                                                                                                                                      
TTTTACGCATATCAAAGTATTCCATACTCGCTCCCGGCCCGTTCTTTTCATTGTTATCGTTCAGGTAACCGTGGATGGTTATCGGCAACTCGGGTGATTTACGTTAGTGGTGATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAAACCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATT  >  NC_000913/1824913‑1825211

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: