Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,252,6570CT40.4% 60.7 / 24.9 52L320F (CTT→TTT) yeiIputative kinase
Reads supporting (aligned to +/- strand):  ref base C (7/24);  new base T (21/0);  total (28/24)
Fisher's exact test for biased strand distribution p-value = 7.73e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGC  >  NC_000913/2252513‑2252804
                                                                                                                                                  |                                                                                                                                                         
cAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAg                                                                                                                                                     <  1:895966/151‑1 (MQ=255)
cAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGGATTTAACTTTTTTgt                                                                                                                                                       >  1:890658/1‑148 (MQ=255)
  aCTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTTTATAAAGTTTTTGAAg                                                                                                                                                     >  2:979705/1‑149 (MQ=255)
     gtttgtttATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGAt                                                                                                                                                  <  2:847789/149‑1 (MQ=255)
     gtttgtttATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGAt                                                                                                                                                  <  1:804523/149‑1 (MQ=255)
       ttgtttATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGCGCCGGGATATAACtttttttttggaga                                                                                                                                                 >  1:41325/1‑140 (MQ=255)
        tgtttATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGATACAg                                                                                                                                            <  1:1111155/150‑1 (MQ=255)
                  cccGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGGTATAGCCTTTTTTGAGGGTACAGGTTTcgcgcc                                                                                                                                  >  2:431105/1‑148 (MQ=255)
                   ccGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGTATATAGCTTTCTTTAAGGATACAGTTTTcgcgcc                                                                                                                                  >  2:1098054/1‑147 (MQ=255)
                    cGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCTGGGATATAGCTTTTTTTTAGGATAAAGTTTTcgcggc                                                                                                                                  >  2:149452/1‑146 (MQ=255)
                    cGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCCGGGGTTTTGCTTTTTTTTAGGAAACAATTTTCCCGa                                                                                                                                   >  2:120571/1‑147 (MQ=255)
                    cGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGGATATAGGTTTTTTGAAGGAAAGAGGTTTCcggcca                                                                                                                                  >  2:266498/1‑145 (MQ=255)
                           tCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGTGTATAAGTTTTTTTTAAGGTACAGttttccccacaaccccc                                                                                                                           >  2:183557/1‑134 (MQ=255)
                           tCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGATATATCTTTTTTTTAGGAGACAGTTTTCCCGCCAgacccc                                                                                                                          >  1:891398/1‑143 (MQ=255)
                              gTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGTTTATAGCTTTTTTGTAGGGTAAAGTTTTCGCGACAGCGCCCGcttt                                                                                                                    >  2:579852/1‑150 (MQ=255)
                                 tATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGGATATAGGTTTTTTTAAGGGGACAGTTTTCGCGGCAGCGCCCCGttttcg                                                                                                                  >  1:511808/1‑148 (MQ=255)
                                        gCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGACTGGGGTATAGCTTTCTTTGAGGGTACCAGTTTCGCGACGGCCCCCCttttttggtggc                                                                                                            >  2:730257/1‑140 (MQ=255)
                                          gAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTgcg                                                                                                      <  1:721602/151‑1 (MQ=255)
                                          gAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGATATAGATTTTTTTAAGGGTACAGTTTTCGCGGCAACGCCCGGTTTTCgg                                                                                                                >  2:1102081/1‑142 (MQ=255)
                                                gATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGGATATTATTTTTTTGTATGGTACAGGTTTCGCGACCAGCCCCCCTTTTCGGGGAGccccgcgg                                                                                                     >  2:641852/1‑142 (MQ=255)
                                                     agaACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCTGGGATATAGCTTTTTTTAAGGATACAGATTTCCCGCCAGCGCCCGGTTTTTGGTAGCCCGCGCGGGAAatt                                                                                             >  2:40218/1‑149 (MQ=255)
                                                      gaACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCCGGTGTATAGTTTTTTTTTAGGAAAAAGGTTTCGCGGCAGAGCCCGGTTTTCGGGCTGCTGCGCgggcaact                                                                                             >  2:856283/1‑142 (MQ=255)
                                                       aaCAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCTGGGTTATTGCTTTTTTTTAGGATATAGTTTTCCCGGCCGCGCCCCTTTTGCGGGAGCCTGCGCGGCCAttt                                                                                             >  2:224905/1‑147 (MQ=255)
                                                         cAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTcgcgcg                                                                                       <  2:367739/151‑1 (MQ=255)
                                                            tttttGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGAGCCGGGTTTTAACTTTTTTTGAAGGGACAGGTTTCCCGACAACGCCCCGTTTTTGGGTCCCTGCCGCGGCAATTTctgcggc                                                                                      >  1:1030792/1‑144 (MQ=255)
                                                            tttttGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCTGGGTTATAAATTTTTTTTAAGGTAAAATTTTCGCGACAGCGCCCCTTTTTTGGGAGGCCGCGCGGCAAATTTGCGcccc                                                                                     >  2:710299/1‑150 (MQ=255)
                                                            tttttGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTTTTTAAAGAAAAAGGTTCCCCGACAACGCCCGCTTTTCGGGAGGCCGCGCGGGAATTTTTCGCGcc                                                                                     >  2:3707/1‑150 (MQ=255)
                                                             ttttGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCa                                                                                    <  2:271517/150‑1 (MQ=255)
                                                                       cgcgCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAGCTTa                                                                        <  1:719701/151‑1 (MQ=255)
                                                                          gcCAGCGCATACGACAGTAGACAGCTTTGGTGCTGACGATGGTTTTATGGCGGGCCGCGGGTATAGGTTTTTTTAAGGAAACAGATTCCCCGCCAGCGCCCCATTTGCGGTAGCCCGCGCCGGCATTTTTCCCCCCCGCGGCCGCTTtaa                                                                      >  2:610723/1‑150 (MQ=255)
                                                                            cAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCTGGTATATAGCTTGCTTGAATGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCTAGCGGCAGCTTaaa                                                                      <  1:682609/148‑1 (MQ=255)
                                                                                  aTACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGGATATAGCTTTTTTTTAGGATACAGTTTTCGCGACAACGCCCCGTTTTTGGTAGACCGCGCGGCCATTTTTCGCGCCCGCGGCAGCGTTaaca                                                                    >  2:744049/1‑144 (MQ=255)
                                                                                      gACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTg                                                         <  2:353755/151‑1 (MQ=255)
                                                                                            aGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGGATATAACTTTTTTTTAGGATAAAGTTTTCGCGGCAGCGCCCGTTTTTGGGGAGACTGGGCGGCAATTTTGCGCGCCCGCGGCAGCGTTAAAAAAACCACCCCGTCCGcc                                                   >  1:957562/1‑151 (MQ=255)
                                                                                              aCAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGGATATAGCTTTCTTGTAGGATAAAGGTTTCGCGACAGCGCCCCCTTTTTGGGAGCCTGCGCGGCCATTTTGCGCGCCCGCGGCAGCCTAAAAAACCCTCCCCCGTCTGcc                                                   >  2:333262/1‑149 (MQ=255)
                                                                                                    ttGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAAcg                                            <  2:890923/150‑1 (MQ=255)
                                                                                                         gCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGATACAGTTTCCGCGTCCGGGCCCGTTTTTCGTTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAGCTTAAGCACCCCTTCCCTCTCTGCCGATAACGCGCttt                                      <  1:121572/151‑1 (MQ=255)
                                                                                                           tGACGATGGTTTTATGGCGGGCCCGGGATATAACTTTCTTTTAGGATACAGTTTTCGCGACAGCGCCCCCTTTTCGGTGGCCTGCCCGGCCAATTTTCGCGCCCGCCGCAGCTTAAACAAACCTCCCCCGTTTCCCGATAACGCGCctt                                      >  2:672956/1‑149 (MQ=255)
                                                                                                              cGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTa                                 <  1:156085/151‑1 (MQ=255)
                                                                                                              cGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTa                                 <  1:1097898/151‑1 (MQ=255)
                                                                                                               gATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAg                                <  1:729785/151‑1 (MQ=255)
                                                                                                                     tttATGGCGGGCCTGGTATATAGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCa                           <  2:934925/150‑1 (MQ=255)
                                                                                                                        aTGGCGGGCCTGGTATATAGCGTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTAGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACACGCTTTCATTAGTGCCAATg                        <  1:485573/150‑1 (MQ=255)
                                                                                                                         tGGCGGGCCTGGTATATAGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGgt                      >  2:72212/1‑151 (MQ=255)
                                                                                                                           gCGGGCCTGGTATATAGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGgtgt                    <  2:754917/151‑1 (MQ=255)
                                                                                                                              ggCCTGGTATATAGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTaa                  >  1:705884/1‑150 (MQ=255)
                                                                                                                                   tGGTATATAGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATg             >  2:955778/1‑151 (MQ=255)
                                                                                                                                            aGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCgg      <  2:510575/150‑1 (MQ=255)
                                                                                                                                             gCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGAt    <  1:279101/151‑1 (MQ=255)
                                                                                                                                              cTTTCTAGAATGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTCCCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGcc        <  1:156649/146‑1 (MQ=255)
                                                                                                                                                ttCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATg   <  2:890790/149‑1 (MQ=255)
                                                                                                                                                ttCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATg   <  2:243081/149‑1 (MQ=255)
                                                                                                                                                ttCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGc  <  1:650303/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                         
CAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTCTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCAGCTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTGCCGGATGC  >  NC_000913/2252513‑2252804

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: