Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913599,5760TG40.4% 84.9 / 24.3 58V288G (GTG→GGG) cusAcopper/silver efflux system, membrane component
Reads supporting (aligned to +/- strand):  ref base T (4/30);  new base G (23/0);  total (28/30)
Fisher's exact test for biased strand distribution p-value = 3.31e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATCGACAA  >  NC_000913/599426‑599723
                                                                                                                                                      |                                                                                                                                                   
aaaCGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGggt                                                                                                                                                     <  2:122423/151‑1 (MQ=255)
 aaCGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGggtg                                                                                                                                                    <  1:696009/151‑1 (MQ=255)
 aaCGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGggtg                                                                                                                                                    <  1:369688/151‑1 (MQ=255)
 aaCGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGggt                                                                                                                                                     <  1:287702/150‑1 (MQ=255)
       cgacgaCTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGAt                                                                                                                                               <  2:211434/150‑1 (MQ=255)
        gacgacTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGgggggggggttc                                                                                                                                             >  1:717433/1‑145 (MQ=255)
        gacgacTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATcc                                                                                                                                             <  1:798296/151‑1 (MQ=255)
        gacgacTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATcc                                                                                                                                             >  2:1100258/1‑151 (MQ=255)
         acgacTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCt                                                                                                                                            <  1:558452/151‑1 (MQ=255)
           gacTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGc                                                                                                                                          <  1:188179/151‑1 (MQ=255)
              tttAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGGTcccg                                                                                                                                           >  2:475262/1‑145 (MQ=255)
              tttAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGTGGTCCTTCg                                                                                                                                         >  1:16096/1‑149 (MQ=255)
               ttAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTcc                                                                                                                                             >  2:963029/1‑144 (MQ=255)
                 aaTCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCg                                                                                                                                    <  1:485720/151‑1 (MQ=255)
                     acaTCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTCCTGCGATCCCGcc                                                                                                                                 >  2:369074/1‑149 (MQ=255)
                        tCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGGCCCGCGGACCGGCaaaa                                                                                                                              >  1:871629/1‑150 (MQ=255)
                         cGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTCCTGCGATCCGGCaaaaa                                                                                                                             >  1:1022980/1‑150 (MQ=255)
                            tttAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTCCTGCGATCCGGCaaaaa                                                                                                                             >  1:718456/1‑147 (MQ=255)
                             ttAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGATCCTGCGATCCGGCAAAAACGccc                                                                                                                        >  2:182128/1‑151 (MQ=255)
                                  aGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGaa                                                                                                                   <  1:398851/151‑1 (MQ=255)
                                     cAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTg                                                                                                                <  1:516852/151‑1 (MQ=255)
                                      aGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGa                                                                                                               <  1:742830/151‑1 (MQ=255)
                                         gAAAATGGCGTGCCCGTCTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGTAGGCGCAGTGGCGGCCGCGGGGGTGGTGCCGGGCTCCCGCCAAAAAGccccacgagggaac                                                                                                             >  1:551711/1‑139 (MQ=255)
                                            aaTAGCGTGCCCGTTTATTGACGCGATGTTGCGAATGTTCAGAGTGGCCGGGAGATGCGCCGGGGCTTTGCCGAACTAAACGGCGAAGGTGAAGTGGCGGGCGGGGTGGTGATCCTTTGATCCGGCAAAAACGCCCGAGAAGTGATcgccg                                                                                                         <  1:721890/151‑1 (MQ=255)
                                                gCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGt                                                                                                     <  1:913844/151‑1 (MQ=255)
                                                 cGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTg                                                                                                    <  2:540160/151‑1 (MQ=255)
                                                     cccGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGGGATCCTGCGGTGCGGCAAAAAAGGCCGGGAAGTGATGGGCGCCGTGaa                                                                                                  >  2:913257/1‑149 (MQ=255)
                                                     cccGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTTCTGGCATCCGGCAAAAACGCCCGAGAAGGGATCGccgccgggga                                                                                                  >  2:216228/1‑145 (MQ=255)
                                                     cccGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTCCTGCGGTCCGGCAAAAAAGCCCGGGAAGTGATCGCCGGCGCGAAgg                                                                                                >  1:130541/1‑151 (MQ=255)
                                                       cGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCTGCGATCCGGCAAAAAAGCCCGAGAAGGGATCGCCGCCGTGAAgg                                                                                                >  2:896392/1‑149 (MQ=255)
                                                         tttATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGTGATCCTGCGGTCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACa                                                                                             >  2:917448/1‑150 (MQ=255)
                                                          ttATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACaa                                                                                            <  2:177395/150‑1 (MQ=255)
                                                           tATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACaaa                                                                                           <  2:389318/150‑1 (MQ=255)
                                                                gcgcGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACt                                                                                         <  2:1077561/147‑1 (MQ=255)
                                                                gcgcGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGaa                                                                                     <  2:287489/151‑1 (MQ=255)
                                                                    gATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCTAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGGTCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAAc                                                                                   <  1:901368/149‑1 (MQ=255)
                                                                    gATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGGGTCTGCGATCCGGCCAAAACGCCCGCGAAGTGATCGGCGGCGGGAAGGGCAAACTGGgaa                                                                                    >  1:103100/1‑148 (MQ=255)
                                                                    gATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACg                                                                                  <  1:368495/150‑1 (MQ=255)
                                                                    gATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTCCTGCGGTCCGGCAAAAACGCCCGGGAAGGGGTCGCCGCCCGGAAGGGCAAACTGGAAAAGc                                                                                 >  1:980572/1‑151 (MQ=255)
                                                                     aTGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGTGGTTCTGCGGTCCGGCAAAAACGCCCGGGAAGCGATCGCCGCCCTGAAGGACAAACTGGTAACGc                                                                                 >  2:728120/1‑150 (MQ=255)
                                                                                   cAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGTGATCCTGCGATCCGGCAAAAACGCCCGGGGAGAGATCGCCGCCGTGAAGGAGAAACTGGAAACGCTTAAAAGTAGTCTg                                                                   >  2:264770/1‑150 (MQ=255)
                                                                                   cAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCTGCGATCCGGCAAAAAAGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAAAGCTGAAAagta                                                                        >  2:454664/1‑145 (MQ=255)
                                                                                         ggCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAg                                                            <  1:699397/151‑1 (MQ=255)
                                                                                            ccGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGATGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGc                                                          <  1:65845/150‑1 (MQ=255)
                                                                                               gagaTGCGCCGGGGCATTGCCGACCTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTgg                                                      <  1:152274/151‑1 (MQ=255)
                                                                                                       ccGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTCCTGCGATCCGGCAAAAACGCCCGGGAAGGGATCGCCGCCGTGGAGGAAAAAATGGAAAAGCTGGAAAAGAGTCTGCCGGGAGGCGGGGGGGTAggt                                              >  1:109534/1‑149 (MQ=255)
                                                                                                         ggggCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGTGATCCTGCGATCCGGCAAAAAAGCCCGAGAAGGGATCGCCGCCGGGAAGGACAAACTGGAAAAGCTGAAAAGTAGTCTGCCGGAAGGCGGGGAGATAGtt                                              >  2:127916/1‑149 (MQ=255)
                                                                                                              aTTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGGGATCCTTCGATCCGGCAAAAAAGCCCGAGAAGGGATCGCCGCCGGGAAGGACAAAACGGAAACGCTGAAAAGAAGTCTGCCGGAAGGCGGGGAGATAGTTacaaca                                        >  2:631823/1‑150 (MQ=255)
                                                                                                                  ccGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATAc                                     <  2:467661/149‑1 (MQ=255)
                                                                                                                  ccGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATAc                                     <  2:190181/149‑1 (MQ=255)
                                                                                                                  ccGAACTAAACGGCGAAGGCGAAGTGGCGGCCGGGGGGGGGATCCTTGCAACCGGGAAAAACGCCCGGGAAGTGGTCGCGGCCGTGAAGGACAAACTGGAAACACTGAAAAGTAGACTGCCGGAAGGCGTGGGGATCGTTACAACATACg                                    >  2:685776/1‑150 (MQ=255)
                                                                                                                      aCTAAACGGCGAAGGCGAAGTGGCGGGGGGGGCGGGGAAACGGCGAGGCGGCAAAAACGCCCGAGGAGGGTACGGCCCCGTGAAGGACAAACTGGGAACGCCGTAAAGTAGTCTGCCGGAAGGCGTTGAGATAGTTTATACATACTATCGc                               >  2:842041/1‑151 (MQ=255)
                                                                                                                            cGGCGAAGGTGAAGTTGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAATCGCCCGATAAGTGATCGCCGGCGAGAAGGACAAACTGGAAACGCTGTAAAGTAGTCTGCCGGGAGGTGTGGAGATAGGTACAACATACGATCCcagcca                          <  1:877170/150‑1 (MQ=255)
                                                                                                                             ggcgaaggcgaaGTGGCGGGCGGGGTGGGGATCCTGCGGTCCGGCAAAAACGCCCGAGAAGGGATCGCCGCCGTGAAGGACAAAATAGAAACACTGAAAAGTAGTCTGCCGGGAGGCGGGGAGATAGGTACAACATACGACCGcagcc                           >  2:135195/1‑148 (MQ=255)
                                                                                                                              gcgaaggcgaagTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCt                       <  2:58167/151‑1 (MQ=255)
                                                                                                                                gaaggcgaagTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTc                      <  2:980168/150‑1 (MQ=255)
                                                                                                                                gaaggcgaagTGGCGGGCGGGGGGGGGGTCCCGCGATCCCGCAAAAAAGCCCCAGGAGGGGTCGCCGCCCGGGAGGGCAAAATGGAAAAGCTGGAAAAGAGTCCTCCGGGAGGCGGGGGGATAGTTAAAAAAAAAGGTCGcagcc                           >  1:598993/1‑145 (MQ=255)
                                                                                                                                      gaagTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGAcc               <  2:5059/151‑1 (MQ=255)
                                                                                                                                      gaagTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGAcc               <  2:266426/151‑1 (MQ=255)
                                                                                                                                       aagTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACcg              <  2:465514/151‑1 (MQ=255)
                                                                                                                                                  ggggTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATCGAc    >  1:70078/1‑150 (MQ=255)
                                                                                                                                                  ggggTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATCGACa   <  2:1041765/151‑1 (MQ=255)
                                                                                                                                                   gggTGGTGATCCTGCGATCCGGCAAAAACTCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATCGACaa  <  1:111636/151‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                   
AAACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATCGACAA  >  NC_000913/599426‑599723

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑