Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,265,009 C→A intergenic (+39/‑3) hemA → / → prfA glutamyl tRNA reductase/peptide chain release factor RF‑1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,265,0090CA98.6% 288.2 / NA 73intergenic (+39/‑3)hemA/prfAglutamyl tRNA reductase/peptide chain release factor RF‑1
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base A (39/33);  total (39/34)
Fisher's exact test for biased strand distribution p-value = 4.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGCCTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCGCGCATTATCACGCGAATATGCGCAGTTAAGTGATG  >  NC_000913/1264861‑1265153
                                                                                                                                                    |                                                                                                                                                
aaCTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACt                                                                                                                                                <  2:1008178/151‑1 (MQ=255)
        aaCCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGcc                                                                                                                                        >  1:707096/1‑151 (MQ=255)
        aaCCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGc                                                                                                                                         <  1:152239/150‑1 (MQ=255)
         aCCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCt                                                                                                                                       >  1:827036/1‑151 (MQ=255)
          ccGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCtt                                                                                                                                      <  1:758926/151‑1 (MQ=255)
           cGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTc                                                                                                                                     >  1:260185/1‑151 (MQ=255)
            gCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCt                                                                                                                                    >  2:1079396/1‑151 (MQ=255)
               tGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTAt                                                                                                                                  <  1:930151/150‑1 (MQ=255)
                 aTCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGt                                                                                                                               >  1:653084/1‑151 (MQ=255)
                 aTCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGt                                                                                                                               <  2:469145/151‑1 (MQ=255)
                  tCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGtt                                                                                                                              >  1:903853/1‑151 (MQ=255)
                   ccATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTg                                                                                                                             <  1:380308/151‑1 (MQ=255)
                    cATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGc                                                                                                                            <  1:1105886/151‑1 (MQ=255)
                       gcgcCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCaa                                                                                                                         <  2:414192/151‑1 (MQ=255)
                       gcgcCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCa                                                                                                                          >  1:264337/1‑150 (MQ=255)
                           cAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTg                                                                                                                     <  2:123942/151‑1 (MQ=255)
                            aaCGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTgg                                                                                                                    >  2:656602/1‑151 (MQ=255)
                                aaaTCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGc                                                                                                                >  1:949338/1‑151 (MQ=255)
                                    cACTTCAACAGGCCGCCCGTGACGGGGATAACGAACTCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTg                                                                                                            <  2:200051/151‑1 (MQ=255)
                                     aCTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGc                                                                                                           >  2:1043535/1‑151 (MQ=255)
                                        tCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCa                                                                                                          <  1:851120/149‑1 (MQ=255)
                                             aGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGc                                                                                                   >  2:260402/1‑151 (MQ=255)
                                             aGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGc                                                                                                   <  2:899862/151‑1 (MQ=255)
                                                 cgccCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATg                                                                                               <  1:744555/151‑1 (MQ=255)
                                                   cccGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATga                                                                                              >  1:159225/1‑150 (MQ=255)
                                                   cccGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATga                                                                                              >  1:781859/1‑150 (MQ=255)
                                                    ccGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATga                                                                                              >  1:205039/1‑149 (MQ=255)
                                                          cGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTc                                                                                      <  2:401196/151‑1 (MQ=255)
                                                             ggATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAgg                                                                                   <  1:477776/151‑1 (MQ=255)
                                                                 aacgaacgCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGtt                                                                               <  1:38854/151‑1 (MQ=255)
                                                                       cgCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTggg                                                                         <  2:310836/151‑1 (MQ=255)
                                                                        gCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGt                                                                        >  2:809153/1‑151 (MQ=255)
                                                                          cTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTgg                                                                          >  1:158117/1‑147 (MQ=255)
                                                                              atatTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGAcgcg                                                                  <  2:177062/151‑1 (MQ=255)
                                                                              atatTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGAcgcg                                                                  >  1:225684/1‑151 (MQ=255)
                                                                                  tCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCaa                                                               >  2:53551/1‑150 (MQ=255)
                                                                                      cgcgACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTAt                                                          >  1:674533/1‑151 (MQ=255)
                                                                                        cgACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCg                                                        >  1:563739/1‑151 (MQ=255)
                                                                                         gACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGc                                                       >  1:414095/1‑151 (MQ=255)
                                                                                          aCAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGcc                                                      <  1:254984/151‑1 (MQ=255)
                                                                                          aCAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGcc                                                      <  2:677401/151‑1 (MQ=255)
                                                                                           cAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCg                                                     <  1:344539/151‑1 (MQ=255)
                                                                                             gCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGAc                                                   >  1:570696/1‑151 (MQ=255)
                                                                                              ccTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGAcc                                                  <  1:781653/151‑1 (MQ=255)
                                                                                              ccTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGAcc                                                  <  2:514401/151‑1 (MQ=255)
                                                                                                   ggCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGa                                              >  2:17434/1‑150 (MQ=255)
                                                                                                     cTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACg                                           >  2:902624/1‑151 (MQ=255)
                                                                                                       ggAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGtt                                         >  2:767449/1‑151 (MQ=255)
                                                                                                         aGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGtttt                                       >  2:791526/1‑151 (MQ=255)
                                                                                                             gCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTcgcg                                   >  1:464520/1‑151 (MQ=255)
                                                                                                                 tACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCGCGCAtt                               >  1:879662/1‑151 (MQ=255)
                                                                                                                   catcatTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCGCGCATTAt                             >  1:435693/1‑151 (MQ=255)
                                                                                                                      catTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCGCGCATTATCAc                          >  2:709083/1‑151 (MQ=255)
                                                                                                                         tttCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCGCGCATTATCAcgc                        <  2:75831/150‑1 (MQ=255)
                                                                                                                           tCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCGCGCATTATCACGCGa                      <  1:301414/150‑1 (MQ=255)
                                                                                                                            cTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCGCGCATTATCACGCGAat                    >  1:131436/1‑151 (MQ=255)
                                                                                                                              ttttttACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCGCGCATTATCACGCGAatat                  >  1:27633/1‑151 (MQ=255)
                                                                                                                               tttttACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCGGAAGGGGAAGGCCGGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCGCGCATTATCACGCGAatat                  <  2:1004528/150‑1 (MQ=255)
                                                                                                                               tttttACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCGCGCATTATCACGCGAATATg                 >  2:41962/1‑151 (MQ=255)
                                                                                                                                 tttACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCGCGCATTATCACGCGAATATgc                <  1:761346/150‑1 (MQ=255)
                                                                                                                                   tACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCGCGCATTATCACGCGAATATgcgc              >  2:693555/1‑150 (MQ=255)
                                                                                                                                    aCAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCGCGCATTATCACGCGAATATgcgc              >  1:892139/1‑149 (MQ=255)
                                                                                                                                     cAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCGCGCATTATCACGCGAATATGCGCAGt           >  2:340820/1‑151 (MQ=255)
                                                                                                                                      aGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCGCGCATTATCACGCGAATATGCGCAGtt          >  1:918114/1‑151 (MQ=255)
                                                                                                                                         gTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCGCGCATTATCACGCGAATATGCGCAGTTa         >  2:136856/1‑149 (MQ=255)
                                                                                                                                         gTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCGCGCATTATCACGCGAATATGCGCAGTTa         >  1:763951/1‑149 (MQ=255)
                                                                                                                                           gCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCGCGCATTATCACGCGAATATGCGCAGTTAAGt      <  1:314811/150‑1 (MQ=255)
                                                                                                                                           gCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCGCGCATTATCACGCGAATATGCGCAGTTAAGt      <  1:694731/150‑1 (MQ=255)
                                                                                                                                            cATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCGCGCATTATCACGCGAATATGCGCAGTTAAGTGa    <  1:737293/151‑1 (MQ=255)
                                                                                                                                             aTTTACGCCTACGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGAATGAACTCCATGAAGAAGTTCAGGCCTTTCTGGGTGACGCGCAACCTAGCGCCGACCAGGAACTTGTTCGCGCATTATCACGCGAATATGCGCAGTTAAGt      <  1:1112476/148‑1 (MQ=255)
                                                                                                                                             aTTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGATCGTTTTCGCGCATTATCACGCGAATATGCGCAGTTAAGTGAt   <  1:96817/151‑1 (MQ=255)
                                                                                                                                               ttACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCGCGCATTATCACGCGAATATGCGCAGTTAAGTTATg  <  1:872554/150‑1 (MQ=255)
                                                                                                                                               ttACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCGCGCATTATCACGCGAATATGCGCAGTTAAGTGATg  <  2:1037248/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
AACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGCCTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCGCGCATTATCACGCGAATATGCGCAGTTAAGTGATG  >  NC_000913/1264861‑1265153

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: