Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,621,118 | 0 | A | C | 42.1% | 99.0 / 11.9 | 59 | V7G (GTG→GGG) | eamA | cysteine and O‑acetyl‑L‑serine efflux system |
Reads supporting (aligned to +/- strand): ref base A (23/10); new base C (0/24); total (23/36) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 2.86e-08 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
ACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGATTTT > NC_000913/1620971‑1621268 | aCTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACAccc > 1:8512/1‑151 (MQ=255) cTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACAcccc > 2:634132/1‑151 (MQ=255) ggTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAG‑CCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCttt > 1:15981/1‑151 (MQ=255) gTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTc < 2:1033024/150‑1 (MQ=255) tGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTcgc > 1:528562/1‑151 (MQ=255) tGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTcgc > 1:522512/1‑151 (MQ=255) gACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGAc > 2:28814/1‑151 (MQ=255) aaaaGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCt < 1:232932/151‑1 (MQ=255) aaaGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTa < 1:587088/151‑1 (MQ=255) ggAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTg > 1:346956/1‑150 (MQ=255) aaGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACCCCCCATCTTTTCGCGACATTCTACCGCCTCTGAAttt < 1:112143/151‑1 (MQ=255) aaGCGACCAGCATAAAGCGAAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTCCCAGTAGCGCCAACCCCCCATCTTTTCGCGACATTCTACCGCCTCTGAAttt < 2:533232/151‑1 (MQ=255) cGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCAt > 1:737793/1‑151 (MQ=255) aCCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAACCCCCACTTTGATGACCCAAAAATTTAGCCCCCATACGCCCACTCCCAGTAGCGCCAACCCCCCATCTTTCCGCGACATTCTCCCGCCTCTGAATTTCAt < 2:212833/149‑1 (MQ=255) ccAGCATAAACCGCAACCCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCCCTCCCAGTAGCGCCAACCCCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCt < 2:775942/150‑1 (MQ=255) cAGCATAAAGCGCAACCCGGCCAGCACCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTACCCCCCATCCGCCCACTCCCAGTAGCGCCACCCCCCCACCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCtt < 2:528955/150‑1 (MQ=255) cATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGt > 1:910410/1‑151 (MQ=255) aTAAAGCGCAACCCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCCCTTTGATGACCACAAAATTTAGCCCCCATACGACCCCTACCAGTAGCGCCAACCCCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTg < 1:598921/149‑1 (MQ=255) aaggccaaacccggccggCAACAGCGGTGCCATTTTATAAAGCCCCCCTTGTATGCCCAAAAAATTAAGCCCCCATCCGACCACTGCCATGAGCGCCAACCCCCCATCTTTCCCCGACATTCTACCGCCTCTGAATTTCATCTTTTg < 1:641245/137‑1 (MQ=255) aaaGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCCCTTTGATGACCCCAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGt < 1:685080/148‑1 (MQ=255) ggCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACCCCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAg < 1:103409/151‑1 (MQ=255) ggCCAGCATCAGCGGTGCCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATCCGACCACTCCCAGTAGCTCCACCCCCCCATCTTTCCGCACCATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACt < 2:574786/148‑1 (MQ=255) gCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGc > 2:814978/1‑151 (MQ=255) ccAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCt > 1:1033708/1‑151 (MQ=255) cgcatccggCGGTGGCATATAATGAAGCCCGATTTGGATGGCCGCAAAATTGAGCCCCCATACGCCCACGACAAGGAGCTCCATCTCCCCACCTTTTCCCAACATTGGACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAg < 2:458315/140‑1 (MQ=255) cAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTg > 1:523608/1‑151 (MQ=255) cAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCAAAAAATTTAGCCCCCATACGACCCCTCCCAGTGGCGCCAACCCCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAg < 1:955963/148‑1 (MQ=255) accAGCGGTGGCAGGTTATGAGGCCCCTCTTTGATGCCCACAAAATTGAGCCCCCTTACGGCCATTACCAGTAGCGCCAACACCCCATCTTTTCGCGATATTCAACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAAtt < 1:108885/149‑1 (MQ=255) aTCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTCCCAGTAGCGCCAACCCCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAAt < 1:170345/150‑1 (MQ=255) cAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAAttt > 2:594553/1‑150 (MQ=255) aGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTAc > 1:422800/1‑151 (MQ=255) gCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACt > 2:887565/1‑151 (MQ=255) ggTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACttt > 2:652867/1‑151 (MQ=255) ggTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTACCCCCCATACGCCCACTACCAGTAGCGCCACCACCCCATCTTTCCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACtt < 1:244725/150‑1 (MQ=255) ggCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGCCCACTACCAGTAGCGCCAACCCCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCt < 1:246422/151‑1 (MQ=255) aTGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCttt > 2:383932/1‑150 (MQ=255) gTTATGAAGCCCCACTTTGATGCCCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACCCCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTaa < 1:979209/150‑1 (MQ=255) aTGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGt > 1:821902/1‑151 (MQ=255) aaGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGCCCACTACCAGTAGCGCCAACCCCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAg < 2:698522/147‑1 (MQ=255) aaGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCTCTCCCAGTAGCGCCAACCCCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGt < 1:1075829/148‑1 (MQ=255) aaGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCCCTACCAGTAGCGCCAACCCCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTg < 1:490638/150‑1 (MQ=255) ccccATTTTGATGACCACAAAATTTAGCCCCCATACGACCACTCCCAGTAGCGCCAACCCCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGAtt < 1:766538/150‑1 (MQ=255) ccccACTTTGATGACCACAAAATTTACCCCCCATACGCCCCCTCCCAGTAGCCCCAACCCCCCATTTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTc < 2:820915/151‑1 (MQ=255) cccTCTTTGATGACCACAAAATTTAGCCCCCATACGCCCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCg < 2:158241/151‑1 (MQ=255) cccACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTCCCAGTAGCGCCAACCCCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCg < 1:896537/151‑1 (MQ=255) cccACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCg > 1:463128/1‑151 (MQ=255) tttGATGACCACAAAATTTAGCCCCCATACGCCCACTACCAGTAGCGCCAACCCCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGtt < 1:694241/148‑1 (MQ=255) aaaaaaTTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCCCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTa < 1:285189/148‑1 (MQ=255) gCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCAtt > 2:326724/1‑150 (MQ=255) cccccATCCGACCGCTACCAGTAGCGCCAACCCCCCATCTTTCCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCAt < 1:787386/148‑1 (MQ=255) cccccATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTa > 2:992104/1‑150 (MQ=255) cATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATgcg > 1:499402/1‑151 (MQ=255) aCTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAg < 2:1010884/150‑1 (MQ=255) ccAGTAGCGCCAACCCCCCATCTTTTCGCGAAATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTc < 2:756345/148‑1 (MQ=255) ccAGAAGCCCAAACGCCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGc < 1:1026088/150‑1 (MQ=255) gcgcCAACCCCCCATCTTTTCGCGACTTTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCtata < 2:924665/148‑1 (MQ=255) gccccAACCCCCCATCTTTCCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCtata < 2:66588/145‑1 (MQ=255) cgcCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACtt < 2:15980/150‑1 (MQ=255) ccAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCgg > 2:70684/1‑151 (MQ=255) cacCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGAtt < 1:687995/150‑1 (MQ=255) acCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGAtttt > 2:869865/1‑151 (MQ=255) | ACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAGCATAAAGCGCAAACCGGCCAGCATCAGCGGTGGCATGTTATGAAGCCCCACTTTGATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAACAGTTGATTCGTTAGTCGCCGGTTACGACGGCATTAATGCGCAAATAAGTCGCTATACTTCGGATTTT > NC_000913/1620971‑1621268 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |