Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,206,435	0	C	T	41.2%	75.8 / 41.6	68	intergenic (‑179/‑28)	ttdR/ttdA	transcriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (9/31);  new base T (28/0);  total (37/31)
Fisher's exact test for biased strand distribution p-value = 2.39e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTGACAGAGATTGTCGC‑TAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCT  >  NC_000913/3206289‑3206581                                                                                                                                                    |                                                                                                                                                     gtATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGa                                                                                                                                                  <  1:499675/151‑1 (MQ=255)  tATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGa                                                                                                                                                  >  2:336619/1‑150 (MQ=255)       cGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAAt                                                                                                                                             <  1:615336/150‑1 (MQ=255)                  gCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGa                                                                                                                                 <  1:444606/151‑1 (MQ=255)                   ccATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAg                                                                                                                                <  2:265622/151‑1 (MQ=255)                    cATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AAATTTTTCAGACAAAAACAAAAAATTGAGt                                                                                                                               >  1:1023811/1‑151 (MQ=255)                     aTTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAAAAAAAAAAATGGAGtt                                                                                                                              >  1:1055681/1‑151 (MQ=255)                     aTTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGAAAAATACAAAAAATGGAg                                                                                                                                >  1:1049958/1‑149 (MQ=255)                     aTTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGCGAAATTTTTCCA‑ACAAAAACAAAAACATGAGtt                                                                                                                              >  2:861945/1‑151 (MQ=255)                       tCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AAATTTTTCAGACAAAAAAAAAAAATGGAGTTg                                                                                                                             >  1:24522/1‑150 (MQ=255)                        cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAAAACAAAAAATGGAGTTGcc                                                                                                                           >  1:46928/1‑151 (MQ=255)                              tctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AAATTTTTCACAAAAAAACAAAAACTTGAGtt                                                                                                                              >  2:856840/1‑142 (MQ=255)                               ctGGCGCTATATTCATTCTTATTTTCGCGTTGAATCGCGAACTGTTAATGACATATTCACGGTGGTAAAAAATATAAAACCCCATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATGCAGAAGCAGGAGTGGCCatgat                                                                                                                      <  2:467781/149‑1 (MQ=255)                                tGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTT‑AATTTTTTTCAACAAAAACAAAAAATAGAGGTGACat                                                                                                                         >  2:268748/1‑145 (MQ=255)                                    gcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAATACAAAAAATTGGGTTGcccag                                                                                                                        >  1:269083/1‑139 (MQ=255)                                    gcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAAAACAAAAACTGGAGTTGCCATGATGAGCg                                                                                                                 >  2:913213/1‑149 (MQ=255)                                     cAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGaaa                                                                                                              <  1:257605/151‑1 (MQ=255)                                      aaTATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAg                                                                                                             <  2:195593/151‑1 (MQ=255)                                         attcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAAAACAAAAAATGGAGTTGCCATGATGAGCGAAAgga                                                                                                           >  1:813992/1‑148 (MQ=255)                                         attcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGtaa                                                                                                          <  2:906533/151‑1 (MQ=255)                                            cattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAAACAAAAAAAAAAACTGGAGGTGCCCTTGTGAGCGAAAGgaa                                                                                                          >  2:670323/1‑148 (MQ=255)                                               tcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGc                                                                                                     <  2:689299/150‑1 (MQ=255)                                                  tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCCAACAAAAAAAAAAACTGGAGGTGCCCTGATGAGCGAAAAGAATAAACaa                                                                                                   >  2:500795/1‑149 (MQ=255)                                                  tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCa                                                                                                    <  2:96653/148‑1 (MQ=255)                                                  tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCa                                                                                                    <  2:718530/148‑1 (MQ=255)                                                      ttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGc                                                                                              <  2:242553/150‑1 (MQ=255)                                                        cgcgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGt                                                                                           <  1:1038473/151‑1 (MQ=255)                                                         gcgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAATACAAAAAAATGAGTTGCCCTGATGAGCGGAAGgaaaaaaaaa                                                                                                   >  1:801332/1‑138 (MQ=255)                                                           gTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAAACAAAAAAAAAAAATTGAGTTGGCATGATGGGGGAAAATAATAAACAAAAGGGAGTg                                                                                          >  2:132971/1‑149 (MQ=255)                                                              gATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAT‑aa                                                                                    <  2:505269/151‑1 (MQ=255)                                                              gATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAT‑aa                                                                                    <  1:563227/151‑1 (MQ=255)                                                                  cACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTg                                                                                <  1:248831/151‑1 (MQ=255)                                                                   aCGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAAAAAAAAAAATGGAGTTGCCATGATGAGCGAAAGTAATAAGCAAAAGGCAGTGAAT‑aa                                                                                    >  2:814470/1‑146 (MQ=255)                                                                     gAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTGACa                                                                             <  1:50380/151‑1 (MQ=255)                                                                      aaCTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAAAAAAATAAAAAAACTGGAGGTGCCCTGATGAGGGAGAGGAATAAAAAAAAAGAAGTGAAT‑aa                                                                                    >  2:120634/1‑143 (MQ=255)                                                                             aaTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AAATTTTTCAAACAAAAAAAAAAAATAGGGGTGGCCTGATGAGCGAAAAAAATAAATAAAAAGCAGTGAAA‑AAATTGGCagag                                                                          >  2:236943/1‑146 (MQ=255)                                                                              aTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAAAAAAAAAAATGGAGGTGCCATGATGAGCGGAAAGAATAAACAACAGGCAGTGAAT‑AAGTTGACAGAGAtt                                                                       >  1:497629/1‑148 (MQ=255)                                                                               ttACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AAATTTTTCAGACAAAAACAAAAAAATGAGGTGGCATGATGAGGGTAAGTAATAAGCAACAGGTAGGGTAT‑AAGGTGGAAGAGATTggt                                                                    >  2:150919/1‑148 (MQ=255)                                                                                tACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTGACAGAGATTGTCg                                                                   <  2:536750/150‑1 (MQ=255)                                                                                tACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AAATTTTTCAGACCAATAAAAAAAATGGGGTTGTCATGGTGTGCGGAAGGAAAAAGCAAAACGCCCTGGAT‑AAAGTGTCAGAAATTGttg                                                                   >  2:251038/1‑148 (MQ=255)                                                                                 aCATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTGACAGAGATTGTCGc                                                                  <  1:387520/150‑1 (MQ=255)                                                                                  cATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAAAACAAAAACTGGAGTTGCCATGATGAGCGAAAGGAATAAACAAAAGGCAGGGAAT‑AAGTTGACAGAGATTGTCGC‑t                                                                >  1:829048/1‑150 (MQ=255)                                                                                  cATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTGACAGAGATTGTCGC‑Ta                                                               <  1:196201/151‑1 (MQ=255)                                                                                   atatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AAATTTTTCAAACAAAAAAAAAAAATGGAGGTTGCATTGTGGGGGGAAGTAATAAACAACAGGGAGGGAAT‑AAATTTAAAGAGAATGTCGC‑Ta                                                               >  2:857325/1‑150 (MQ=255)                                                                                          cGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTGACAGAGATTGTCGC‑TAACTTTAcc                                                       <  2:646483/151‑1 (MQ=255)                                                                                                      atatAAACCCACATTTTTAGTGGTAGTTTGTGGCGGTA‑AATTTTTCAAGACAAATACAAAAACGGGAGTTGCCATGATGAGCGAAAGTAATAACAAACAGGCAGTGAAT‑AAGTTGACAGAGATTGTCGC‑TAACTTTACCGCCATGATTTCt                                           <  2:512/151‑1 (MQ=255)                                                                                                         taAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAAACAAAAAAAAAAAATGGAGTTTTCATGATGAGGGAAAGGAATAAGCAAAAAGCAGGGAAT‑AAGTTGGCAGAGATTTTCGG‑TAACTTTTCCGCCATGAttttt                                           >  2:532676/1‑146 (MQ=255)                                                                                                               cacaTTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCCAAACAAA‑AAAAAAAATTGAGTTTCCCTGATGAGGGGAAATAAAAAAAAAAAAGAAAGGAAA‑AAAGTGAAAAAGATTGTCGC‑TAACTTTAACGCCCTGATTTTTAACCGaa                                    >  1:500935/1‑149 (MQ=255)                                                                                                                  aTTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAAAACAAAAAATGGAGTTGGCATGATGAGCGGAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTGACAGAGATTGTCGC‑TAACTTTACCGCCATGATTTTTACCCGAATGcc                                >  1:683464/1‑150 (MQ=255)                                                                                                                   tttttAGTGGTATTTTGTGGCGGTG‑AATTTTTCCAGAAAAATAAAAAAACGGGAGTTGCCATGAGGGGCGAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTGACAGAGTTTTTCGC‑TACCTTTACCGCCATGATTTCTACCCGAATGCCt                               <  2:991535/150‑1 (MQ=255)                                                                                                                   tttttAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAATACAAAAAATGGAGTTGCCCTGATGAGCGAAAGTAATAAGCAACAAGCAGGGAAT‑AAGGTGACAGAGATTGTCGC‑TAACTTTACCGCCCTAATTTTTACCCGAATGCCt                               >  1:737664/1‑150 (MQ=255)                                                                                                                    ttttAGTGGTAGTTTGTGGAGGTA‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCAAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTGACAGAGATTGTCGC‑TACCTTTACCGCCATGATTTCTACCCGAATGCCtg                              <  1:750344/150‑1 (MQ=255)                                                                                                                      ttAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTGACAGAGATTGTCGC‑TAACTTTACCGCCATGATTTCTACCCGAATGCCtgat                            <  2:1055493/150‑1 (MQ=255)                                                                                                                        aGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAATACAAAAAATGGAGTTGCCATGATGAGCGGAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTGACAGAGATTGTCGC‑TAACTTTTACGCCATGATTTTTTCCCGAATGCCtgat                            >  1:871520/1‑148 (MQ=255)                                                                                                                         gTGGTAGTTTGTGGCGGAG‑GATTTTTTCTGACAAATAAAAAAACTGGAGGTGGCATGGGGGGGGAAAGAAAAAAACAACAGGCAGTGAAT‑AAGTTGGAAGAGATTGTTGCGCAATTTTACC‑CCCTGATTTTTACACCAATACCtgataa                          >  2:363592/1‑147 (MQ=255)                                                                                                                           ggTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTGACAGAGATTGTCGC‑TAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgtg                      <  1:756364/151‑1 (MQ=255)                                                                                                                             tAGTTTGTGGCGGTG‑AATTTTTTCAGACAAAAACAAAAACCGGAGTTGGCATGATGAGCGAAAATAATAAACAAAAAGCAGGGAAA‑AAAATGACAGAGATTGTTGC‑TAACTTTACCGCCATGATTTCTACCCGAAAGCCtgatgaagggg                     >  2:486171/1‑145 (MQ=255)                                                                                                                                tttGGGGCGGTG‑AATTTTTCCAGAAAAAGAAAAAAACTGGAGTTGCCATGAGGAGCGAAAGTAATAAGCACCAGGCAGTGAAA‑AAGTTGACAGGGATTGCCGC‑TACCTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGtggtgg                  <  2:123576/150‑1 (MQ=255)                                                                                                                                  tgtgGCGGTG‑AATTTTTCCAGAAAAATACAAAAACTGGAGTGGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTGACAGAGATTGTCGC‑TAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGAt                <  2:320602/150‑1 (MQ=255)                                                                                                                                    tgGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTGACAGAGATTGTCGC‑TAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATaa              >  2:461541/1‑150 (MQ=255)                                                                                                                                    tgGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTGACAGAGATTGTCGC‑TAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATaa              >  1:288966/1‑150 (MQ=255)                                                                                                                                     ggCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTGACAGAGATTGTCGC‑TAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAAc            >  1:390831/1‑151 (MQ=255)                                                                                                                                      gCGGTG‑AATTTTTCCAGACAAATACAAAAACGGGGGTGGCCATGACGCGCGAAAGTAAAAAAAAAGAGGAAG‑GAATAAAGAGGACGGAGATTGTGTC‑TAAGTTTCCCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATa               <  2:297751/147‑1 (MQ=255)                                                                                                                                         gTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTGACAGAGATTGTCGC‑TAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaa         >  2:934667/1‑150 (MQ=255)                                                                                                                                          tG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTGACAGAGATTGTCGC‑TAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaa        >  2:484058/1‑150 (MQ=255)                                                                                                                                           g‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTGACAGAGATTGTCGC‑TAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAAc      >  2:376968/1‑151 (MQ=255)                                                                                                                                             aaTTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTGACAGAGATTGTCGC‑TAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaa        <  1:12383/148‑1 (MQ=255)                                                                                                                                                  ttCCAGACAAATAAAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTGACAGAGATTGTCGC‑TAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCt  <  2:480757/149‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                     GTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAT‑AAGTTGACAGAGATTGTCGC‑TAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCT  >  NC_000913/3206289‑3206581

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑