| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,601,176 | 0 | T | C | 46.6% | 54.1 / 32.8 | 58 | E304G (GAA→GGA) | ftsX | putative ABC transporter permease |
| Reads supporting (aligned to +/- strand): ref base T (24/7); new base C (0/27); total (24/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.48e-10 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGTTCCGAAAA‑CCTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGGGCGCAGGATGAATCCATCTGTCGCACCAATCAGTTTCTGT > NC_000913/3601028‑3601316 | ttattCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGTTCCGAAAA‑CCTGTGCCACTTc < 2:560218/150‑1 (MQ=255) attCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGTTCCGAAAA‑CCTGTGCCACTTCCGc < 2:429896/151‑1 (MQ=255) attCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGTTCCGAAAA‑CCTGTGCCACTTCCGc > 1:33579/1‑151 (MQ=255) ggCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGTTCCGAAAA‑CCTGTGCCACTTCCGCAAcc < 2:1036408/150‑1 (MQ=255) ggCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGTTCCGAAAA‑CCTGTGCCACTTCCGCAAcc > 1:584479/1‑150 (MQ=255) aGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGTTCCGAAAA‑CCTGTGCCACTTCCGCAACCGCCGATg > 1:343292/1‑150 (MQ=255) gCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGTTCCGAAAA‑CCTGTGCCACTTCCGCAACCGCCGATGACAAt > 1:840113/1‑151 (MQ=255) aaaTGTTGTCCCGGGGCAACCCCCGCTCCCCCCCAGCCAATCATCGAGCATACCAGCAGAAATAGCAGCCATTCAACAAATGATAACCCATTAATATAAACCTTCGTCCCAAAAC‑CCTGTCCCCCTCCCGCACCCGCCGATGACAATCgc < 1:250380/150‑1 (MQ=255) aaTGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGTTCCGAAAA‑CCTGTGCCACTTCCGCAACCGCCGATGACAATCgc > 1:233939/1‑149 (MQ=255) ttggccggTGGCAACCCCCCCGCCCCCCCAACCAATCATCGACCACCCCAGCAGCAATACCAGGCATTCATCGAATAAAACGCAATTAATAAAAACCTTCGTACCAAAAA‑CCGGTCCCCCTCCCGAAACCGCCGATGACAATCgcagca < 2:655928/142‑1 (MQ=255) tACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGTTCCGAAAA‑CCTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCAcc > 2:645955/1‑148 (MQ=255) tACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGTTCCGAAAA‑CCTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCAcc > 2:226167/1‑148 (MQ=255) gTGGAAACCCACGCGCCCCCCCACCAAATCACCGAGCATCCCAGAAGAAATAGCAGGCATTCATCAAATGAAAAGCCATTGATAAAAAACTTCGTCCCAAAAC‑CCTGTGCCACTCCCACAACCGCCGATGACAATCGCAGCACCAg < 1:674552/146‑1 (MQ=255) aaGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGTTCCGAAAA‑CCTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGAc > 1:1007179/1‑151 (MQ=255) ccACGCTCCCCCCCAGCAAATCACCGACCATCCCAGAAAAAAAAGCAGGCATTCATCAAATGAAAAGCCATTAATATAAACCTTCGTCCCAAAAC‑CCTGTCCCACTCCCACAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACaa < 1:901563/150‑1 (MQ=255) cccccccccacccaATCACCGACCAACCCAGCAGCAATAGCAGGCATTAACCAAATGATAAGCAATTGATATCAACCTCCTTCCCAAACC‑CCTGTCCCCTTCCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAAt < 1:487611/142‑1 (MQ=255) cTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGTTCCGAAAA‑CCTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTa > 1:68932/1‑150 (MQ=255) ccccccAGCCAATCATCGACCATCCCAGCAACAAAGGCAGGCATTCACCGAATAAAAAGCCATTAAAATCAACTTTCTTCCCAAAAA‑CCTGTGCCCCTCCCCCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAAt < 1:249740/146‑1 (MQ=255) cccAGCAAATCAACGAGCATACCAGAAAGAAGAACGGGCATTCATAGAAGGATAAGCATTTGATACAAATCTCCGTCCCAAAAG‑CCGGGCCCACTCCCGAAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGaca < 1:613177/150‑1 (MQ=255) cccAACAAATCACCGAGCATCCCAGAAGAAATAGCAGGCTTTCATCGAATGATAAGCATTTAATATAAAACTTCGTTCCAAAAC‑CCGGTGCCCTTCCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGac < 1:305910/149‑1 (MQ=255) ccaaacaaaaaatcGAGCATACCAGCAACAAGCGCGGGCATTCATCAAATAAAAAGCATTTGATATAAAACTTCGTCCCAAAAC‑CCTGTCCCTCCTCCACAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGacaa < 2:313526/140‑1 (MQ=255) aaTCATCAACCATCCCAGCAGAAATAGCGGGCATTCATCAAATAATAAGCTATTAATAAAAATCTTCGTCCCAAAAC‑CCTGTGCCCCTCCCGAAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATg < 1:183656/149‑1 (MQ=255) catcGAGCATACCAGCAGAAAAAGCAGGCATTCATAAAATAAAAAGCCATTAATAAAAACCTCCGTCCCAAAAC‑CCTGTCCCCCTCCCGAAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATgcg < 2:879077/148‑1 (MQ=255) caccGACCACACCAGCAACAATAGCAGGTTTTCATCAAATGATAAGCCTTTAATATAAATCTTCGTCCCAAAAC‑CCTCGCCCCCTTCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATg < 1:849206/143‑1 (MQ=255) atcccAGCAGAAATAGCAGGCATTCATCGAATAAAAAGCTATTGATATCAACCTTCGTCCCAAAAC‑CCTGTGCCCCTCCCGAAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATgc < 1:45768/136‑1 (MQ=255) ccAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCTTTGATATAAACCTTCGTCCCAAAAC‑CCTGTCCCTCTCCCCAAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATccc < 1:588243/149‑1 (MQ=255) agcagaAATAGCAGGCTTTCATGAAATAAAAACCAATTTATATAAACCTTCTTCCCAAAAC‑CCTGTGCCACTCCCGAAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGaaaa < 1:734121/143‑1 (MQ=255) aaaagcagcCATTCAAAGAATAATAAGCTATTAATATCAACCTTC‑TTCCAAAAACCCTGTCCCACTCCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTgc < 1:895661/146‑1 (MQ=255) aaTAGCGGGCATTCATCAAATAATAAGCCATTAATATAAACCTTCGTCCCAAAAC‑CCTGTCCCCCTCCCAAAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTg < 1:51117/148‑1 (MQ=255) tAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGTTCCGAAAA‑CCTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGcc > 1:690981/1‑150 (MQ=255) gCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGTTCCGAAAA‑CCTGTGCCACTTCCGCAACCGCCGATGACAATCGAAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGccacc > 2:633354/1‑151 (MQ=255) ggaaTTCATCAAATAAAAACCCATTGAAATCAACCTTCTTTCAAAAAC‑CCTGTCCCCCTCCCGAAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGcca < 2:829643/143‑1 (MQ=255) aTTCATCGAATGATAAGCCATTGATATCAAACTTCGTTCCGAAAA‑CCTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATAc > 1:791923/1‑149 (MQ=255) ttCATCGAATGATAAGCCATTGATATCAAACTTCGTTCCGAAAA‑CCTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAgg > 1:391741/1‑151 (MQ=255) aTCAAATGAAAACCCTTTGATATAAACCTTCTTCCCAAAAC‑CCTGTCCCACTCCCCCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGa < 1:824871/149‑1 (MQ=255) aTAACCCATTTATATAAACCTTCGTCCCAAAAC‑CCTGTCCCACTCCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGGGCGCAg < 1:500607/151‑1 (MQ=255) aaGCCATTGATATCAAACTTCGTTCCGAAAA‑CCTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGGGCGCAgg > 1:800312/1‑150 (MQ=255) gCCATTGATATCAAACTTCGTTCCGAAAA‑CCTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGGGCGCAGGATg > 2:1066848/1‑151 (MQ=255) ctaTTAATATCAAACTTCGTCCCGAAAC‑CCGGCCCCCCTCCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGGGCGCAGGAt < 1:513101/147‑1 (MQ=255) ccATTGATATCAAACTTCGTTCCGAAAA‑CCTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGGGCGCAGGATg > 1:909162/1‑150 (MQ=255) ttGATATCAAACTTCGTTCCGAAAA‑CCTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGGGCGCAGGATGAATc > 2:499557/1‑151 (MQ=255) taatatAAAACTTC‑TTCCAAAAACCCTGTCCCCCTCCCCAAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGGGCGCAGGATGAATc < 1:429904/148‑1 (MQ=255) tGATATCAAACTTCGTTCCGAAAA‑CCTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGGGCGCAGGATGAATcc > 1:288160/1‑151 (MQ=255) gATATCAAACTTCGTTCCGAAAA‑CCTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGGGCGCAGGATGAATcc > 1:972970/1‑150 (MQ=255) atatCAAACTTCGTTCCGAAAA‑CCTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGGGCGCAGGATGAATCCa > 1:601232/1‑150 (MQ=255) tatCAAACTTCGTTCCGAAAA‑CCTGTGCCACTTCCGCAACCGCCGATGA‑AATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGGGCGCAGGATGAATCCATc > 2:315017/1‑150 (MQ=255) acAACCTTCGTACCAAAAC‑CCTGCCCCCCTTCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGGGCGCAGGATGAATCCATCt < 1:858177/149‑1 (MQ=255) cAAACTTCGTTCCGAAAA‑CCTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGGGCGCAGGATGAATCCATCTGt > 2:332493/1‑151 (MQ=255) aaaCTGCGTCCCAAAAC‑CCTGTCCCCTTCCCACAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGGGCGCAGGATGAATCCATCTGTc < 2:825544/151‑1 (MQ=255) acctccgtccaaaaac‑CCTGTCCCCCTCCCGAAGCCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGGGCGCAGGATGAATCCATCt < 2:584347/135‑1 (MQ=255) accTTCGTCCCAAAAC‑CCTGTGCCACTCCCCCAACCGCCGATGACAATCGCAGCACCTGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGGGCGCAGGATGAATCCATCTGTCg < 1:676544/149‑1 (MQ=255) ttcaaaaac‑CCTGTCCCCCTCCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGGGCGCAGGATGAATCCATCTGTCGCACCaa < 1:998692/145‑1 (MQ=255) ttCCAAAAC‑CCTGTGCCACTCCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGGGCGCAGGATGAATCCATCTGTCGCa < 1:11515/146‑1 (MQ=255) ccGAAAA‑CCTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGGGCGCAGGATGAATCCATCTGTCGCACCAATc > 2:582461/1‑150 (MQ=255) aaag‑CCTGTGCCCCTCCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGGGCGCAGGATGAATCCATCTGTCGCAc < 1:671560/142‑1 (MQ=255) aaac‑CCTGTCCCCCTCCCCCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGGGCGCAGGATGAATCCATCTGTCGCACCAATCa < 1:468366/148‑1 (MQ=255) aaacctgaccccACTTCCGAAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGGGCGCAGGATGAATCCATCTGTCGCACCAATCa < 2:354680/138‑1 (MQ=255) cTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGGGCGCAGGATGAATCCATCTGTCGCACCAATCAGTTTCTGt > 1:43449/1‑151 (MQ=255) | TTATTCAGGCGTAAAGTGGCGTAAATGTTGTACCGTGGCAAGCCACGCTGCCACCCAGCCAATCATCGAGCATACCAGCAGCAATAGCAGGCATTCATCGAATGATAAGCCATTGATATCAAACTTCGTTCCGAAAA‑CCTGTGCCACTTCCGCAACCGCCGATGACAATCGCAGCACCAGAATTTCTGACAAAATTAATGACAACAATGCGCCAGAAAATCCCAGCAGTGCGCCACCATACAGGAACGGGCGCAGGATGAATCCATCTGTCGCACCAATCAGTTTCTGT > NC_000913/3601028‑3601316 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |