Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,097,581	0	A	G	43.8%	111.3 / 24.3	73	noncoding (72/88 nt)	serX	tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base A (5/36);  new base G (32/0);  total (37/36)
Fisher's exact test for biased strand distribution p-value = 9.74e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATAC‑GTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAA  >  NC_000913/1097436‑1097730                                                                                                                                                  |                                                                                                                                                        cACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTc                                                                                                                                                      <  1:1034905/149‑1 (MQ=11)      cGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGAGGGGGGGGGTCGAAcccc                                                                                                                                               >  2:779188/1‑151 (MQ=16)         tcgctgtggcgccAACCGGCTGCCACCCGTTGTCGAACCCCGGTCCGTTCTTGATATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAAccc                                                                                                                                                <  2:978632/136‑1 (MQ=11)         tCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCg                                                                                                                                             <  2:741616/150‑1 (MQ=37)              gTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATAC‑Gt                                                                                                                                      <  2:382073/151‑1 (MQ=40)                  gTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATAC‑Gtt                                                                                                                                     <  1:848172/148‑1 (MQ=255)                     cAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATAC‑GTTGCCGta                                                                                                                               <  2:234113/151‑1 (MQ=255)                      aaCTGGCTATCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTTGAAACCCCCATAC‑GTTTcc                                                                                                                                  >  1:1027355/1‑147 (MQ=11)                         tGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGAGAGGGGGGGGTTTGCACCCCCCCTAC‑CTTTCCggtttc                                                                                                                            >  2:172275/1‑145 (MQ=11)                           gCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTCGCAACCCCCATAC‑GTTTCCGTATtcac                                                                                                                          >  1:75713/1‑150 (MQ=14)                            cTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTTGAACCCCCCCTAC‑GGTTCCGcat                                                                                                                              >  2:483308/1‑145 (MQ=11)                            cTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGGTTGAACCCCCGAGAC‑GGTGCCGTATccaca                                                                                                                         >  1:1007507/1‑150 (MQ=14)                             tGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGGTCGAAACCCCCATAC‑ggttcccgatac                                                                                                                            >  1:691551/1‑135 (MQ=11)                              gTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATAC‑GTTGCCGTATACACA‑Ctt                                                                                                                     <  2:685432/151‑1 (MQ=255)                                     gTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATAC‑GTTGCCGTATACACA‑CTTTCCAgg                                                                                                               <  1:37075/150‑1 (MQ=255)                                          cGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATAC‑GTTGCCGTATACACA‑CTTTCCAGGCGTGc                                                                                                          <  2:50732/150‑1 (MQ=255)                                           gAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGGGGGGGGGGTTCGAACCCCCCCTAC‑GTTTCCGTATAAACA‑CCTTTCAcgcggggcc                                                                                                        >  1:943632/1‑146 (MQ=14)                                           gAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAAGGGGGGGGTTGAACCCCCCCTACAGTTG‑CGTATAAACA‑CTTTTCAGGCgcggtc                                                                                                        >  1:30357/1‑146 (MQ=11)                                               cccGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCCCTTCTTTAAATATGGCGGGGAGGGGGGGGGTTGACCCCCCCCAAC‑GCTGTCGCATACACA‑CTTTTCACGCGTGCTcc                                                                                                       >  1:473483/1‑148 (MQ=14)                                                ccGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGAGAGGGGGGGAATCTAAACCCCCCTAC‑GTTTCCGTCTACACA‑AATTCCCGGGGGGCTcc                                                                                                       >  2:1033215/1‑147 (MQ=14)                                                 cGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATAC‑GTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAg                                                                                                  <  1:869593/151‑1 (MQ=255)                                                  ggTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGGTTGGACCCCCCATAC‑GTTTCCGTATACACA‑CTTTTCCGGCGTGCTCCTTCAGc                                                                                                 >  2:87981/1‑151 (MQ=17)                                                  ggTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTAAGGGGGGGATTCGGAACCCCGATAA‑GTTTCCGGAAACACACCTTTCCA‑GCGTGCTcccctcgc                                                                                                 >  1:443378/1‑145 (MQ=14)                                                      ggggCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATAC‑GTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACt                                                                                             <  2:890006/151‑1 (MQ=255)                                                      ggggCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATAC‑GTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACt                                                                                             <  2:152219/151‑1 (MQ=255)                                                      ggggCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATAC‑GTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACt                                                                                             <  1:358058/151‑1 (MQ=255)                                                       gggCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATAC‑GTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTc                                                                                            <  1:990986/151‑1 (MQ=255)                                                       gggCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATAC‑GTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTc                                                                                            <  1:319324/151‑1 (MQ=255)                                                        ggCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTTGAACCCCCGCTAC‑GTTTCCGTATAAACA‑CATTTCGGGCGTGCTCCCTCAGCCACTc                                                                                            >  1:436272/1‑150 (MQ=17)                                                        ggCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTTGAACCCCCCCTAA‑GTTTCCGTATACAAA‑CTTTCCCAGCGTGGTCCTTCAGCCAc                                                                                              >  2:496191/1‑148 (MQ=17)                                                            tctcATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTTGAACCCCCCCTAC‑GTTGCCGTATACACA‑CCTTCCAGGCGTGCTCCCTCAGCCACTccg                                                                                          >  1:366682/1‑146 (MQ=17)                                                            tctcATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGGTTGAAACCCCCCAAC‑GGTTCCGTATAAAAC‑CTTTCCCCGCGGGCTCCTTCAGCCAccccggc                                                                                        >  2:852052/1‑144 (MQ=14)                                                               cATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATAC‑GTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCt                                                                                    <  2:874416/151‑1 (MQ=255)                                                                 tCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGGTCGAACCCCCCCTAA‑ATTTCCGTATACACA‑CTTTTCCGGCGTGCTCCCTTAGCCCCCCCGACAcc                                                                                     >  1:532268/1‑148 (MQ=17)                                                                  ccccccGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTCGAACCCCCCATAC‑GTTTCCGTATATACA‑CTTTCCAGGCGTGCCCCCTCAGCCCCCCGGACACCCCAc                                                                                 >  1:2878/1‑151 (MQ=17)                                                                  ccccccATTTTGTGCAATATACGAAAAAAAAGCCCGTGCTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGTTATTCGAACCCCCGATAC‑GTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCt                                                                                    <  1:237302/148‑1 (MQ=14)                                                                   cccccGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGGTCGAAACCCCCCTAC‑GTTTCCGGATACACA‑CCTTTCACGCGTGGTCCTTTCGCCACCCGGACACCCCAcc                                                                                >  1:688598/1‑151 (MQ=17)                                                                   cccccGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATAC‑GTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACTCCTCAcc                                                                                <  2:61578/151‑1 (MQ=255)                                                                    ccccGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTTGGAACCCCCATAC‑GGTTTCGTATACACA‑ATTTCCAGGCGGGGGCCTTCTGCCACCCGGGCACCTCAcc                                                                                >  2:619237/1‑150 (MQ=17)                                                                        ggTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATAC‑GTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAAtt                                                                           <  2:712169/151‑1 (MQ=255)                                                                        ggTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATAC‑GTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAAtt                                                                           <  1:982783/151‑1 (MQ=255)                                                                         gtgtgtGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTTGAACCCCCCATAC‑GTTGCCGTATACAAA‑CCTTCCCGGCGTGCTCCCTCCGCCACCCGGACACCTCACCAAAttt                                                                          >  2:1066418/1‑150 (MQ=14)                                                                          tgtgtgCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTTGAAACCCCCACAC‑GTTGCCGTATACACA‑CCTTTCAAGCGGGGTCCTTCAGCCACCCCGGCAccccccccaaat                                                                           >  1:8723/1‑139 (MQ=14)                                                                           gtgtgCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATAC‑GTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTc                                                                        <  1:578101/151‑1 (MQ=255)                                                                               gCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATAC‑GTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTc                                                                    <  2:393643/151‑1 (MQ=255)                                                                                  atatACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTACTTTAAATATGGCGGTGAGGGGGGGGGTCGAACCCCCCATAA‑GTTTCCGTATACAAA‑CCTTCCCGGCGTGCTCCTTTAGCCACTCCGGCCCCCCACCAAAATTTCGt                                                                      >  2:356493/1‑146 (MQ=14)                                                                                     taCGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGAGAGGGGGGGGTTTGAAACCCCCCTAC‑GTTTCCGCATACACA‑CTTTTCCGGCGTGCTCCCTCCGCCACCCCGGCACCTCCCCCAATTTTCGTTCCTGt                                                                >  2:199105/1‑149 (MQ=14)                                                                                       cGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATAC‑GTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTg                                                            <  1:232439/151‑1 (MQ=255)                                                                                         aaaaaaaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGGGAGGGGGGGGTTTGAACCCCCCAAAA‑GTTTCCGGAAACAAA‑CTTTTCAGGTGTGCTCCCTCCCCCACCCGGAACACTCACCAAAATTTTCTTCCTTTCtt                                                             >  1:466953/1‑148 (MQ=2)                                                                                          aaaaaaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGGTCGGACCCCCCATAC‑GGTGCCGTATACACA‑CTTTTCAGGCGTGCTCCTTCAGCCACTCGGACACCCCCCCCAAATTTCGTTTCCGTCTTTCt                                                          >  1:996737/1‑150 (MQ=11)                                                                                             aaaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTTGAAACCCCCCCAC‑TTTTTCGTATAAAAA‑CTTTTCAGGCGGGGTCCTTCAGCCACTCGGACACCCCACCCAATTTTTGTTCCTGTCTTGCTGGa                                                       >  2:991403/1‑150 (MQ=14)                                                                                             aaaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATAC‑GTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGaa                                                      <  1:650976/151‑1 (MQ=255)                                                                                             aaaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAAGGGGGGGGTCGAAACCCCCATAC‑GTTTCCGGATAAACA‑CTTTCCAGGCGTGGTCCTTCAGCCACTCGGGCACCCCAACAAAATTTCGTTCCTGTCtttttgg                                                        >  1:947906/1‑144 (MQ=14)                                                                                              aaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGGTCGAACCCCCCATAC‑GTTGCCGTATACACA‑CTTTCCCGGCGTGCTCCTTCAGCCCCTCGGACACCTCCCCCAATTTTCGTTCCTGTCTTTCt                                                          >  1:860248/1‑146 (MQ=14)                                                                                                   ccGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTCGAACCCCCGATAC‑GTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCCAATTGTCGTTCCTGTCTTGCTGGGACggggg                                                >  1:1014856/1‑149 (MQ=255)                                                                                                    cGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTCGAAACCCCGGTAC‑GTTGCCGTATACACA‑CTTTCCAGGCGTGGTCCTTCCGCCACTCGGACACCCCCCCCAAATGTTGGTCCTGTCTTTCTGGAACGGgcg                                                >  1:566646/1‑150 (MQ=14)                                                                                                        cTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATAC‑GTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTaa                                            <  1:289593/150‑1 (MQ=255)                                                                                                         tttCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGGTTCGAACCCCCCATAC‑GTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCCCCCCGACACCTCACCCAATTTTCGTTCCTGTCTTGCTGGGACGGGCGCCaa                                            >  1:357972/1‑149 (MQ=14)                                                                                                          ttCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATAC‑GTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAAt                                           <  1:631774/149‑1 (MQ=255)                                                                                                              tACGAGCTCTTCTTTAAATATGGCGGTGGGGGGGGGGTTTGAACCCCCCAAAA‑GTTTCCGTATAACTA‑CATTCCAGGGGTGCTCCCTTTGCCCCCCGGACACCCCACCCAAATTTTTTTCCTGTCTTGCTGGAACGGGCGCTCATTTTggg                                     >  1:170264/1‑151 (MQ=9)                                                                                                               aCGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATAC‑GTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGa                                    <  2:692667/151‑1 (MQ=255)                                                                                                                 gAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATAC‑GTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGaaa                                  <  2:283597/151‑1 (MQ=255)                                                                                                                 gAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATAC‑GTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGaaa                                  <  1:1075619/151‑1 (MQ=255)                                                                                                                            tAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATAC‑GTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTg                        <  1:972863/150‑1 (MQ=255)                                                                                                                             aaaTATGGCGGTGAGGGGGGGATTCGAACCCCCGATAC‑GTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAg                      <  2:566503/151‑1 (MQ=255)                                                                                                                               atatGGCGGTGAGGGGGGGATTCGAACCCCCGATAC‑GTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGt                    >  2:586615/1‑151 (MQ=255)                                                                                                                                 atGGCGGTGAGGGGGGGATTCGAACCCCCGATAC‑GTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTc                   <  1:413060/150‑1 (MQ=255)                                                                                                                                  tGGCGGTGAGGGGGGGATTCGAACCCCCGATAC‑GTTGCCGTATACCCA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTca                  <  1:156630/150‑1 (MQ=255)                                                                                                                                         gAGGGGGGGATTCGAACCCCCGATAC‑GTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACtt          <  2:946865/151‑1 (MQ=255)                                                                                                                                           gggggggATTCGAACCCCCGATAC‑GTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACttt         <  1:1054219/150‑1 (MQ=255)                                                                                                                                            ggggggATTCGAACCCCCGATAC‑GTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACttttt       <  2:1048040/151‑1 (MQ=255)                                                                                                                                              ggggATTCGAACCCCCGATAC‑GTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTg      >  1:814746/1‑150 (MQ=255)                                                                                                                                                ggATTCGAACCCCCGATAC‑GTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGaa    >  2:491299/1‑150 (MQ=255)                                                                                                                                                  aTTCGAACCCCCGATAC‑GTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGaaaa  >  1:1044248/1‑150 (MQ=255)                                                                                                                                                  |                                                                                                                                                        CACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCCCGATAC‑GTTGCCGTATACACA‑CTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAA  >  NC_000913/1097436‑1097730

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑