Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,265,009 C→A intergenic (+39/‑3) hemA → / → prfA glutamyl tRNA reductase/peptide chain release factor RF‑1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,265,0090CA98.0% 186.8 / NA 51intergenic (+39/‑3)hemA/prfAglutamyl tRNA reductase/peptide chain release factor RF‑1
Reads supporting (aligned to +/- strand):  ref base C (1/0);  major base A (21/28);  minor base G (0/1);  total (22/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGCCTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCGCGCATTATCACGCGAATATGCGCAGTTAAGTGATG  >  NC_000913/1264861‑1265153
                                                                                                                                                    |                                                                                                                                                
aaCTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACt                                                                                                                                                >  1:804402/1‑151 (MQ=255)
        aaCCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAg                                                                                                                                          <  2:519777/149‑1 (MQ=255)
            gCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCt                                                                                                                                    <  2:245923/151‑1 (MQ=255)
               tGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTa                                                                                                                                   <  1:611515/149‑1 (MQ=255)
               tGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTa                                                                                                                                   <  1:723908/149‑1 (MQ=255)
                   ccATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGtt                                                                                                                              <  1:110082/150‑1 (MQ=255)
                          ccAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACt                                                                                                                      >  1:694047/1‑151 (MQ=255)
                           cAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTg                                                                                                                     <  1:669987/151‑1 (MQ=255)
                            aaCGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTgg                                                                                                                    <  1:92738/151‑1 (MQ=255)
                               gAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGa                                                                                                                   >  2:793795/1‑149 (MQ=255)
                               gAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAg                                                                                                                 <  2:386372/151‑1 (MQ=255)
                                 aaTCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGcc                                                                                                               >  2:1052803/1‑151 (MQ=255)
                                        tCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCAt                                                                                                         <  1:715240/150‑1 (MQ=255)
                                           aCAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAAc                                                                                                     <  1:970949/151‑1 (MQ=255)
                                                ccgccCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCAt                                                                                                <  1:1013013/151‑1 (MQ=255)
                                                  gccCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATg                                                                                               >  1:625489/1‑150 (MQ=255)
                                                       tGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATgaaga                                                                                           >  2:1004688/1‑149 (MQ=255)
                                                         aCGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGtt                                                                                       >  2:559795/1‑151 (MQ=255)
                                                           ggggATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTc                                                                                      >  2:649565/1‑150 (MQ=255)
                                                                          cTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTg                                                                       >  2:1010635/1‑150 (MQ=255)
                                                                           tGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGAc                                                                     >  2:181681/1‑151 (MQ=255)
                                                                                     gcgcGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCaaa                                                              <  2:918163/148‑1 (MQ=255)
                                                                                         gACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGc                                                       >  2:939997/1‑151 (MQ=255)
                                                                                         gACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGc                                                       <  2:745948/151‑1 (MQ=255)
                                                                                            aGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCg                                                     >  2:294622/1‑150 (MQ=255)
                                                                                            aGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCg                                                     >  2:1032733/1‑150 (MQ=255)
                                                                                                   ggCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGaa                                             <  1:727358/151‑1 (MQ=255)
                                                                                                            aGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTcg                                     <  1:583063/150‑1 (MQ=255)
                                                                                                               aGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCgcgc                                  >  1:273612/1‑150 (MQ=255)
                                                                                                               aGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCGCGCa                                 <  2:441795/151‑1 (MQ=255)
                                                                                                                gTACATCATTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGCTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCGCGCAt                                <  2:322380/151‑1 (MQ=255)
                                                                                                                     tcatTTTCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAATTGGAAGCCCTGCATGTACTCCATGAAGAAGTTCAGGCGTTGCTGGGCGGCGCGCAATCTATCGCCGACCAGGAACGTTTTCGCGCATTAt                             >  2:181227/1‑149 (MQ=255)
                                                                                                                         tttCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCGCGCATTATCAcgc                        <  2:827826/150‑1 (MQ=255)
                                                                                                                           tCTTTTTTTACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCGCGCATTATCACGCGa                      <  2:375831/150‑1 (MQ=255)
                                                                                                                              ttttttACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCGCGCATTATCACGCGaag                    <  2:871756/149‑2 (MQ=255)
                                                                                                                              ttttttACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCGCGCATTATCACGCGaa                     <  2:993493/148‑1 (MQ=255)
                                                                                                                               tttttACAGGGTGCATTTACGCCTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCGCGCATTATCACGCGAATATg                 >  1:574181/1‑151 (MQ=255)
                                                                                                                               tttttACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCGCGCATTATCACGCGAATATg                 >  2:824160/1‑151 (MQ=255)
                                                                                                                                ttttACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCGCGCATTATCACGCGAATATgc                >  2:1023290/1‑151 (MQ=255)
                                                                                                                                 tttACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCGCGCATTATCACGCGAATATgcg               >  2:589518/1‑151 (MQ=255)
                                                                                                                                  ttACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCGCGCATTATCACGCGAATATgcg               <  1:675161/150‑1 (MQ=255)
                                                                                                                                   tACAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCGCGCATTATCACGCGAATATgcgc              <  2:187126/150‑1 (MQ=255)
                                                                                                                                    aCAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCGCGCATTATCACGCGAATATGCGCAg            <  2:673931/151‑1 (MQ=255)
                                                                                                                                     cAGGGTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCGCGCATTATCACGCGAATATGCGCAGt           >  1:339835/1‑151 (MQ=255)
                                                                                                                                        ggTGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCGCGCATTATCACGCGAATATGCGCAGTTaa        <  1:981787/151‑1 (MQ=255)
                                                                                                                                          tGCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCGCGCATTATCACGCGAATATGCGCAGTTAAg       >  2:71579/1‑150 (MQ=255)
                                                                                                                                           gCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCGCGCATTATCACGCGAATATGCGCAGTTAAGt      <  2:1070114/150‑1 (MQ=255)
                                                                                                                                           gCATTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCGCGCATTATCACGCGAATATGCGCAGTTAAGTg     <  1:236180/151‑1 (MQ=255)
                                                                                                                                            cATTTACTGCTATGAATCCTTCTATCGTTGCCATACTGGAAGCCCTGCATGCACGCGATGAAGAAGTTCAGGCGATGCTTGGTGCCGCGCAAACTATCGCCGACCAGGTACGTTTTCGCGCATTATCACGTGAATATGCGCAGTTAAGt      <  2:420684/149‑1 (MQ=255)
                                                                                                                                             aTTTACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCGCGCATTATCACGCGAATATGCGCAGTTAAGTGAt   <  1:264382/151‑1 (MQ=255)
                                                                                                                                              tttACGACTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCGCGCATTATCACGCGAATATGCGCAGTTAAGTGATg  >  2:534004/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
AACTGACTAACCGCTTGATCCATGCGCCAACGAAATCACTTCAACAGGCCGCCCGTGACGGGGATAACGAACGCCTGAATATTCTGCGCGACAGCCTCGGGCTGGAGTAGCAGTACATCATTTTCTTTTTTTACAGGGTGCATTTACGCCTATGAAGCCTTCTATCGTTGCCAAACTGGAAGCCCTGCATGAACGCCATGAAGAAGTTCAGGCGTTGCTGGGTGACGCGCAAACTATCGCCGACCAGGAACGTTTTCGCGCATTATCACGCGAATATGCGCAGTTAAGTGATG  >  NC_000913/1264861‑1265153

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑