Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,756,217	G→T	D174Y (GAC→TAC)	pykF →	pyruvate kinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,756,217	0	G	T	100.0%	291.2 / NA	74	D174Y (GAC→TAC)	pykF	pyruvate kinase I
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (42/32);  total (42/32)

TGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCG  >  NC_000913/1756072‑1756361                                                                                                                                                  |                                                                                                                                                 tGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAATACaa                                                                                                                                              <  2:720910/150‑1 (MQ=255)      aTGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAATACAAACAgg                                                                                                                                         <  2:807762/150‑1 (MQ=255)       tGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAATACAAACAGGAc                                                                                                                                       >  1:1058663/1‑151 (MQ=255)        ggTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAATACAAACAGGAcc                                                                                                                                      >  1:701436/1‑151 (MQ=255)            tGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAATACAAACAGGACCTGAt                                                                                                                                  >  1:944293/1‑151 (MQ=255)             gATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAATACAAACAGGACCTg                                                                                                                                    <  1:808719/148‑1 (MQ=255)             gATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAATACAAACAGGACCTGATc                                                                                                                                 <  1:850782/151‑1 (MQ=255)              aTCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAATACAAACAGGACCTGATCt                                                                                                                                <  1:672754/151‑1 (MQ=255)                  gTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAATACAAACAGGACCTGATCTTTgg                                                                                                                            >  2:95531/1‑151 (MQ=255)                    aTGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAATACAAACAGGACCTGATCTTTGGtt                                                                                                                          <  1:806347/151‑1 (MQ=255)                            tACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAATACAAACAGGACCTGATCTTTGGTTGCGAACa                                                                                                                   >  2:341284/1‑150 (MQ=255)                             aCCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAATACAAACAGGACCTGATCTTTGGTTGCGAACa                                                                                                                   >  1:949815/1‑149 (MQ=255)                             aCCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAATACAAACAGGACCTGATCTTTGGTTGCGAACAAg                                                                                                                 <  1:452088/151‑1 (MQ=255)                              ccGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAATACAAACAGGACCTGATCTTTGGTTGCGAACAAgg                                                                                                                <  1:930013/151‑1 (MQ=255)                                  cATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAATACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGt                                                                                                             <  2:98524/150‑1 (MQ=255)                                  cATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAATACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGt                                                                                                             >  1:521405/1‑150 (MQ=255)                                   aTTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAATACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAg                                                                                                           <  2:590303/151‑1 (MQ=255)                                      gAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAATACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACt                                                                                                        >  2:1010495/1‑151 (MQ=255)                                      gAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAATACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACt                                                                                                        <  2:110152/151‑1 (MQ=255)                                        aGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAATACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACttt                                                                                                      <  1:984734/151‑1 (MQ=255)                                          gTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAATACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTttgt                                                                                                    >  2:333770/1‑151 (MQ=255)                                            aaCAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAATACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTttgtt                                                                                                   <  1:447186/150‑1 (MQ=255)                                            aaCAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAATACAAACAGGACCTGATCTTTGGGTGCGAACAAGGCGTAGACTttgttg                                                                                                  >  2:37055/1‑151 (MQ=255)                                               aaaGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAATACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTtgctg                                                                                               <  1:593451/151‑1 (MQ=255)                                                aaGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAATACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTtgctgc                                                                                              >  1:951264/1‑151 (MQ=255)                                                 aGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAATACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTgctgct                                                                                             <  2:193354/151‑1 (MQ=255)                                                 aGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAATACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTgctgct                                                                                             >  1:548866/1‑151 (MQ=255)                                                   ttATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAATACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTc                                                                                           >  2:328121/1‑151 (MQ=255)                                                    tATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAATACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTcc                                                                                          >  2:812939/1‑151 (MQ=255)                                                    tATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAATACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTc                                                                                           <  2:283788/150‑1 (MQ=255)                                                         gTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAATACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTa                                                                                      <  2:609936/150‑1 (MQ=255)                                                         gTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAATACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTAt                                                                                     >  1:57320/1‑151 (MQ=255)                                                              gTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAATACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTa                                                                                <  1:989381/151‑1 (MQ=255)                                                                 cTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAATACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGc                                                                             >  1:530673/1‑151 (MQ=255)                                                                  tGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAATACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCg                                                                            >  1:1047402/1‑151 (MQ=255)                                                                  tGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAATACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCg                                                                            >  1:231626/1‑151 (MQ=255)                                                                   gAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAATACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCg                                                                            >  1:236824/1‑150 (MQ=255)                                                                   gAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAATACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCg                                                                            <  1:657926/150‑1 (MQ=255)                                                                   gAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAATACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGt                                                                           >  1:908831/1‑151 (MQ=255)                                                                        acGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAATACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTg                                                                       >  2:208126/1‑150 (MQ=255)                                                                        acGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAATACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGa                                                                      <  1:312382/151‑1 (MQ=255)                                                                               ccTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAATACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTAt                                                                <  2:959685/150‑1 (MQ=255)                                                                                  gggCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAATACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCg                                                              <  1:850452/149‑1 (MQ=255)                                                                                     cGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAATACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATc                                                         >  2:970698/1‑151 (MQ=255)                                                                                      gAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAATACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATcc                                                        >  1:515027/1‑151 (MQ=255)                                                                                      gAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAATACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATcc                                                        >  2:675211/1‑151 (MQ=255)                                                                                       aaaaCAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAATACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCg                                                       <  1:319064/151‑1 (MQ=255)                                                                                       aaaaCAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAATACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCg                                                       >  1:353821/1‑151 (MQ=255)                                                                                       aaaaCAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAATACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCg                                                       >  1:836166/1‑151 (MQ=255)                                                                                             aaGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAATACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCa                                                 <  2:1006284/151‑1 (MQ=255)                                                                                                gtgtGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAATACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCt                                              >  1:827306/1‑151 (MQ=255)                                                                                                    gAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAATACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGaa                                           >  1:423018/1‑150 (MQ=255)                                                                                                     aaCCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAATACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGaa                                           >  1:394500/1‑149 (MQ=255)                                                                                                      aCCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAATACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAgc                                        >  1:686122/1‑151 (MQ=255)                                                                                                      aCCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAATACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAgc                                        <  2:1029343/151‑1 (MQ=255)                                                                                                          gcctgGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAATACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCAc                                    >  2:187649/1‑151 (MQ=255)                                                                                                           cctgGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAATACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCAcg                                   >  2:378594/1‑151 (MQ=255)                                                                                                           cctgGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAATACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCAcg                                   <  1:756052/151‑1 (MQ=255)                                                                                                              ggCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAATACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCAcggcg                                >  2:65176/1‑151 (MQ=255)                                                                                                                cGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAATACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACggcggc                              >  1:535276/1‑151 (MQ=255)                                                                                                                 gTTTCCATTGCTCTGCCAGCACTGGCTGAAAAATACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGgcggcg                             >  2:60901/1‑151 (MQ=255)                                                                                                                   ttCCATTGCTCTGCCAGCACTGGCTGAAAAATACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGa                            <  2:418099/150‑1 (MQ=255)                                                                                                                     ccATTGCTCTGCCAGCACTGGCTGAAAAATACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGaaa                          <  1:922183/150‑1 (MQ=255)                                                                                                                            tctGCCAGCACTGGCTGAAAAATACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCcac                  >  2:414512/1‑151 (MQ=255)                                                                                                                             ctGCCAGCACTGGCTGAAAAATACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCcac                  >  1:241556/1‑150 (MQ=255)                                                                                                                             ctGCCAGCACTGGCTGAAAAATACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCca                   <  1:312306/149‑1 (MQ=255)                                                                                                                              tGCCAGCACTGGCTGAAAAATACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCAcat                >  1:622458/1‑151 (MQ=255)                                                                                                                               gCCAGCACTGGCTGAAAAATACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCAcat                <  2:163320/150‑1 (MQ=255)                                                                                                                                ccAGCACTGGCTGAAAAATACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCAcatca              <  2:60837/151‑1 (MQ=255)                                                                                                                                  aGCACTGGCTGAAAAATACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACatcatc            >  1:721208/1‑151 (MQ=255)                                                                                                                                          cTGAAAAATACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAAt    >  1:376457/1‑151 (MQ=255)                                                                                                                                            gAAAAATACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATc   <  1:287581/150‑1 (MQ=255)                                                                                                                                             aaaaaTACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCg  >  2:512430/1‑150 (MQ=255)                                                                                                                                             aaaaaTACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCg  >  1:912632/1‑150 (MQ=255)                                                                                                                                                  |                                                                                                                                                 TGACGATGGTCTGATCGGTATGGAAGTTACCGCCATTGAAGGTAACAAAGTTATCTGTAAAGTGCTGAACAACGGTGACCTGGGCGAAAACAAAGGTGTGAACCTGCCTGGCGTTTCCATTGCTCTGCCAGCACTGGCTGAAAAAGACAAACAGGACCTGATCTTTGGTTGCGAACAAGGCGTAGACTTTGTTGCTGCTTCCTTTATTCGTAAGCGTTCTGACGTTATCGAAATCCGTGAGCACCTGAAAGCGCACGGCGGCGAAAACATCCACATCATCTCCAAAATCG  >  NC_000913/1756072‑1756361

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑