Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913993,9170AC41.4% 107.3 / 17.6 59V43G (GTG→GGG) ssuBaliphatic sulfonate ABC transporter ATPase
Reads supporting (aligned to +/- strand):  ref base A (32/2);  new base C (0/24);  total (32/27)
Fisher's exact test for biased strand distribution p-value = 2.53e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGCCTCCTTCAA  >  NC_000913/993770‑994061
                                                                                                                                                    |                                                                                                                                                
aGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCccacca                                                                                                                                               <  1:526903/151‑1 (MQ=255)
  cAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCaccacc                                                                                                                                             <  1:599568/151‑1 (MQ=255)
    gACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGAGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGc                                                                                                                                           >  2:477162/1‑151 (MQ=255)
     aCGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGcc                                                                                                                                          >  2:694898/1‑151 (MQ=255)
      cgcgCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGcc                                                                                                                                          >  1:548492/1‑150 (MQ=255)
      cgcgCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGcc                                                                                                                                          >  2:39089/1‑150 (MQ=255)
        cgcATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCcac                                                                                                                                        >  2:1087475/1‑150 (MQ=255)
        cgcATCCTGAAACATCATGCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTCCTTTTGCCACCACCGCTGCGGCCCACCACCGCca                                                                                                                                         <  2:555478/149‑1 (MQ=255)
           aTCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAAc                                                                                                                                    >  2:72241/1‑151 (MQ=255)
                 aaaCATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCt                                                                                                                              >  2:180536/1‑151 (MQ=255)
                   aCATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGc                                                                                                                            >  1:619104/1‑151 (MQ=255)
                    catcatTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGcc                                                                                                                           >  1:638377/1‑151 (MQ=255)
                       catTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCgg                                                                                                                         >  1:312666/1‑150 (MQ=255)
                        atTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGa                                                                                                                        >  1:364267/1‑150 (MQ=255)
                            gcgTGTCTTCTTGATTTTCAGCCGGCGGTGTGGGCCCCGCAAACACTCCGCCGGCGGTTGGTTTTTCCAGCCCGGCCAGCAGGCGCAGCAGGGTACTTTTCCCCCCCCCGCGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGa                                                                                                                        <  1:1112085/146‑1 (MQ=255)
                             cgTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGt                                                                                                                  >  1:218007/1‑151 (MQ=255)
                             cgTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGt                                                                                                                  >  1:47109/1‑151 (MQ=255)
                              gtgtCTTCCTGGATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGt                                                                                                                  >  2:758616/1‑150 (MQ=255)
                              gtgtCTCCCTGATTTTCAGCCAGGGGGGGGGGGCCCGCAAACACTCCGCCGGGGGTGGGCTTTCCCAGCCCGGCCAGCAGGCGCAGCAGGGTATTTTTCCCCCCCCCGCTGCGGCCCCCCACCGCCACAAACTGACCTGCCGGAat                                                                                                                      <  1:935011/146‑1 (MQ=255)
                               tgtCTTCTTAAATTTCAGCCGGGGGGGTGGCCCCCGCCACCCCACCCCCGGCGTTGGGCGTTCCCACCCCTGCCAGCGGGCACAGCGGGTTTTTTTTCCCACCCCCGGGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGaa                                                                                                                       <  2:129949/144‑1 (MQ=255)
                                 tCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAAt                                                                                                              >  1:148072/1‑151 (MQ=255)
                                  cTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATc                                                                                                             >  1:413412/1‑151 (MQ=255)
                                     ccaaaaTTCCAGCCAGGGGTGGGGGCCCCCCTAACCCCCCCCGGGGGGTTGGTTTTCCCACCCCGGCGGGCGGGCGCAGCAGGGTTTTTTGCCCCCCACCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAg                                                                                                          <  2:839762/147‑1 (MQ=255)
                                       tGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGtt                                                                                                        >  1:283916/1‑151 (MQ=255)
                                          aTTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGt                                                                                                     >  1:428382/1‑151 (MQ=255)
                                          aTTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGt                                                                                                     >  1:52848/1‑151 (MQ=255)
                                                 ccgggggTGTGGTGCCCGCAACCCCATCGCGGGGGGTTGGCTTTCCCAGCCCTGCAGGCAGGCGCAGGGGGGTACTTTTCCCCCCCCCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTc                                                                                                   <  1:358838/141‑1 (MQ=255)
                                                  cagcGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACg                                                                                             >  1:211290/1‑151 (MQ=255)
                                                    ggggTGGGGTGCCCGCTACCACACCGCCTGTGGTGGGCTTTCCCAGCCCTGCCAGGAGGCGCAGCGGGGTTTTTTTGCCCCCACCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTc                                                                                                   <  1:121239/141‑1 (MQ=255)
                                                     cGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGat                                                                                           >  2:274784/1‑150 (MQ=255)
                                                         gtggggCCCGATAACCCACCGCGTGCGTTTGGCGTTCCCAGCCCTCCAAACAGGCGAAGCGGGTTTCTTTTCCCACAACCGCCGCGGCCCCCCCCCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGtt                                                                                                    <  2:813542/137‑1 (MQ=255)
                                                           ggtcCCCACTAACACACCGCCTGGGGTTGGCTTTTCCAGCCCTGCCAGCGGGCGCAGCAGGTTTTTTTTCCCCCCCCCGTTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGAc                                                                                              <  1:1084994/141‑1 (MQ=255)
                                                                     accccaccGCCTGGGGTGGGCTTTTCCAGCCCTCCCAGCGGGCGCAGGGGGTTTTTTTCCCCCCCCCCGCGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTaa                                                                              <  2:490183/140‑1 (MQ=255)
                                                                       cccaccGCCGGGGGTTGGCTTTCCCAGCCCTGCCAGCGGCCGCGGAGGGGTCTTTTTCCCCCCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGtt                                                                          <  1:133249/144‑1 (MQ=255)
                                                                       cccaTCGCCGGGGTTTGGCTTTTCCAGCCCTGCCAGCGGGCGCGGGAGGTTCCTTTTCCCCCCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTg                                                                        <  1:207061/149‑1 (MQ=255)
                                                                            cGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTAc                                                                   >  1:60879/1‑151 (MQ=255)
                                                                              ccTGGGGTTGGCTTTCCCAGCCCGGCCAGCAGGCGCGGCAGGTTACTTTTGCCACCCCCGCTGCGGCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACt                                                                  <  1:8170/150‑1 (MQ=255)
                                                                              ccTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTg                                                                 >  1:770078/1‑151 (MQ=255)
                                                                                tGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGc                                                                >  1:349359/1‑150 (MQ=255)
                                                                                 gCGGTTGGCTTTTCCAGCCCTGCCAGCAGGCGCAGCAGGGTACTTTTCCCCCCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGc                                                                <  1:681025/149‑1 (MQ=255)
                                                                                   ggTTGGCTTTCCCAGCCCCGCCAGCAGGCCCAGCAGGTTCTTTTTCCCCCCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCAt                                                              <  1:558645/149‑1 (MQ=255)
                                                                                     ttGGCTTTCCCAGACCTGCCGGCGGGCGCAGGAGGTTCTTTTCGCCCCCCCCGCGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGa                                                           <  1:970976/150‑1 (MQ=255)
                                                                                       ggCGTTCCCAGCCCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCCCCCCCGCGGCGGCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGca                                                        <  1:351863/151‑1 (MQ=255)
                                                                                              ccAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATgg                                                 >  1:105446/1‑151 (MQ=255)
                                                                                                aGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCg                                               >  2:699438/1‑151 (MQ=255)
                                                                                                  cccTGCCGGCAGGCGAGGAAGGTTTCTTTCGCCCCCCCCGCGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATg                                                  <  2:817885/145‑1 (MQ=255)
                                                                                                  aCCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTg                                             >  1:450400/1‑151 (MQ=255)
                                                                                                  aCCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTg                                             >  1:232109/1‑151 (MQ=255)
                                                                                                   ccTGCCAGCAGGCCCAGCGGGGTCCTTTTCCCCCCCCCGCTGCGCCCCCCCCCCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGt                                              <  1:646045/149‑1 (MQ=255)
                                                                                                             ggCGCAGCGGGTTCTTTTTCCCACCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTc                                    <  1:390601/149‑1 (MQ=255)
                                                                                                                      ggttatttttTCCCCCCCCTCAGCGCCCCGCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGt                         <  1:545921/145‑1 (MQ=255)
                                                                                                                      gggtcttttcGCCCCCCCCGCGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAg                          <  1:134624/144‑1 (MQ=255)
                                                                                                                        gttattttGCCCCCCCCCGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTa                        <  1:922636/147‑1 (MQ=255)
                                                                                                                             ttccccacccCCGCGGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCAt                   <  2:494047/146‑1 (MQ=255)
                                                                                                                                 ccaccaCCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGt              >  2:165276/1‑151 (MQ=255)
                                                                                                                                  ccccccccGCTGCGCCCCCCCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGt              <  1:925331/145‑1 (MQ=255)
                                                                                                                                     caccGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGcc          >  1:122043/1‑151 (MQ=255)
                                                                                                                                       ccgccTGCGCCCCCCCCCCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGcct         <  2:647925/147‑1 (MQ=255)
                                                                                                                                             gcgcCCCCCCCCCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGcctcct      <  2:759996/148‑1 (MQ=255)
                                                                                                                                              cgcccccccccCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGCCTCCTTCa   <  2:975149/147‑1 (MQ=255)
                                                                                                                                               ggCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGCCTCCTTCaa  >  1:814522/1‑150 (MQ=255)
                                                                                                                                               ggCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGCCTCCTTCaa  >  2:1100730/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                
AGCAGACGCGCATCCTGAAACATCATTCGCGTGTCTTCCTGAATTTCAGCCAGCGGTGTGGTGCCCGCTAACACATCGCCTGCGGTTGGCGTTTCCAGACCTGCCAGCAGGCGCAGCAGGGTACTTTTGCCACCACCGCTGCGGCCCACCACCGCCACAAACTGACCTGCCGGAATATGTAAATCCAGTTGGTTCAGGACGATATTTTCCGCGTAATGTTTGCTTACTGCATTGAGCAACAATGGCGTGCCCTGGTTCAGACGAGCAGTATTCATACCGTGGCCTCCTTCAA  >  NC_000913/993770‑994061

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: