Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,535,7850AG43.6% 83.3 / 12.2 55F25S (TTC→TCC) yddEPhzC‑PhzF family protein
Reads supporting (aligned to +/- strand):  ref base A (3/28);  new base G (24/0);  total (27/28)
Fisher's exact test for biased strand distribution p-value = 1.18e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGTGACCGCAAATCGGCACTTCAACCGTTGGCGTAAAGTAGCGGATGCGCACATCGCTGTCGTCGCTGTGCAGCAGAAAAGCAGTTTCCGAATGGCCTAACTCGCGGGCGATAAGCTGCATTTGCGCTTCGCTAAGATTATCGGCGGGGAAAACAACACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGACTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTG  >  NC_000913/1535636‑1535933
                                                                                                                                                     |                                                                                                                                                       
cgTGACCGCAAATCGGCACTTCAACCGTTGGCGTAAAGTAGCGGATGCGCACATCGCTGTCGTCGCTGTGCAGCAGAAAAGCAGTTTCCGAATGGCCTAACTCGCGGGCGATAAGCTGCATTTGCGCTTCGCTAAGATTATCGGCGGGGaa                                                                                                                                                        <  1:790545/151‑1 (MQ=255)
 gTGACCGCAAATCGGCACTTCAACCGTTGGCGTAAAGTAGCGGATGCGCACATCGCTGTCGTCGCTGTGCAGCAGAAAAGCAGTTTCCGAATGGCCTAACTCGCGGGCGATAAGCTGCATTTGCGCTTCGCTAAGATTATCGGCGGGGaaa                                                                                                                                                       <  2:339643/151‑1 (MQ=255)
    aCCGCAAATCGGCACTTCAACCGTTGGCGTAAAGTAGCGGATGCGCACATCGCTGTCGTCGCTGTGCAGCAGAAAAGCAGTTTCCGAATGGCCTAACTCGCGGGCGATAAGCTGCATTTGCGCTTCGCTAAGATTATCGGCGGGGAAaaca                                                                                                                                                    <  2:114336/151‑1 (MQ=255)
     ccGCAAATCGGCACTTCAACCGTTGGCGTAAAGTAGCGGATGCGCACATCGCTGTCGTCGCTGTGCAGCAGAAAAGCAGTTTCCGAATGGCCTAACTCGCGGGCGATAAGCTGCATTTGCGCTTCGCTAAGATTATCGGCGGGGgaaaaa                                                                                                                                                    >  2:136588/1‑148 (MQ=255)
       gCAAATCGGCACTTCAACCGTTGGCGTAAAGTAGCGGATGCGCACATCGCTGTCGTCGCTGTGCAGCAGAAAAGCAGTTTCCGAATGGCCTAACTCGCGGGCGATAAGCTGCATTTGCGCTTCGCTAAGATTATCGGCGGGGgaaaa                                                                                                                                                     >  2:247888/1‑146 (MQ=255)
         aaaTCGGCACTTCAACCGTTGGCGTAAAGTAGCGGATGCGCACATCGCTGTCGTCGCTGTGCAGCAGAAAAGCAGTTTCCGAATGGCCTAACTCGCGGGCGATAAGCTGCATTTGCGCTTCGCTAAGATTATCGGCGGGGAGaacaa                                                                                                                                                   >  2:1046067/1‑147 (MQ=255)
         aaaTCGGCACTTCAACCGTTGGCGTAAAGTAGCGGATGCGCACATCGCTGTCGTCGCTGTGCAGCAGAAAAGCAGTTTCCGAATGGCCTAACTCGCGGGCGATAAGCTGCATTTGCGCTTCGCTAAGATTATCGGCGGGGAAAACAACAcc                                                                                                                                               <  1:791340/151‑1 (MQ=255)
          aaTCGGCACTTCAACCGTTGGCGTAAAGTAGCGGATGCGCACATCGCTGTCGTCGCTGTGCAGCAGAAAAGCAGTTTCCGAATGGCCTAACTCGCGGGCGATAAGCTGCATTTGCGCTTCGCTAAGATTATCGGCGGGGgaaaaa                                                                                                                                                    >  2:257686/1‑143 (MQ=255)
            tCGGCACTTCAACCGTTGGCGTAAAGTAGCGGATGCGCACATCGCTGTCGTCGCTGTGCAGCAGAAAAGCAGTTTCCGAATGGCCTAACTCGCGGGCGATAAGCTGCATTTGCGCTTCGCTAAGATTATCGGCGGGGAAAACAACACCgg                                                                                                                                             <  2:481593/150‑1 (MQ=255)
             cGGCACTTCAACCGTTGGCGTAAAGTAGCGGATGCGCACATCGCTGTCGTCGCTGTGCAGCAGAAAAGCAGTTTCCGAATGGCCTAACTCGCGGGCGATAAGCTGCATTTGCGCTTCGCTAAGATTATCGGCGGGGAGAACAAAAcc                                                                                                                                               >  1:1074291/1‑147 (MQ=255)
              ggCACTTCAACCGTTGGCGTAAAGTAGCGGATGCGCACATCGCTGTCGTCGCTGTGCAGCAGAAAAGCAGTTTCCGAATGGCCTAACTCGCGGGCGATAAGCTGCATTTGCGCTTCGCTAAGATTATCGGCGGGGAAAACAACACCGGCAg                                                                                                                                         <  2:399223/151‑1 (MQ=255)
                    tCAACCGTTGGCGTAAAGTAGCGGATGCGCACATCGCTGTCGTCGCTGTGCAGCAGAAAAGCAGTTTCCGAATGGCCTAACTCGCGGGCGATAAGCTGCATTTGCGCTTCGCTAAGATTATCGGCGGGGGAAAAAAAACCCGCAGAAtt                                                                                                                                     >  1:832020/1‑149 (MQ=255)
                     cAACCGTTGGCGTAAAGTAGCGGATGCGCACATCGCTGTCGTCGCTGTGCAGCAGAAAAGCAGTTTCCGAATGGCCTAACTCGCGGGCGATAAGCTGCATTTGCGCTTCGCTAAGATTATCGGCGGGGAAAACAACACCGGCAGAATTGcc                                                                                                                                  <  2:737075/151‑1 (MQ=255)
                         cGTTGGCGTAAAGTAGCGGATGCGCACATCGCTGTCGTCGCTGTGCAGCAGAAAAGCAGTTTCCGAATGGCCTAACTCGCGGGCGATAAGCTGCATTTGCGCTTCGCTAAGATTATCGGCGGGGGAAAAAAAACCCGCAGAAATGcc                                                                                                                                  >  2:233758/1‑147 (MQ=255)
                          gTTGGCGTAAAGTAGCGGATGCGCACATCGCTGTCGTCGCTGTGCAGCAGAAAAGCAGTTTCCGAATGGCCTAACTCGCGGGCGATAAGCTGCATTTGCGCTTCGCTAAGATTATCGGCGGGGGAGACAAAAACAGCAGCAATGCcgcg                                                                                                                              >  1:861841/1‑149 (MQ=255)
                           ttGGCGTAAAGTAGCGGATGCGCACATCGCTGTCGTCGCTGTGCAGCAGAAAAGCAGTTTCCGAATGGCCTAACTCGCGGGCGATAAGCTGCATTTGCGCTTCGCTAAGATTATCGGCGGGGAAAACAAAAACCGCAGGAATTGcg                                                                                                                                  >  1:1075783/1‑145 (MQ=255)
                              gCGTAAAGTAGCGGATGCGCACATCGCTGTCGTCGCTGTGCAGCAGAAAAGCAGTTTCCGAATGGCCTAACTCGCGGGCGATAAGCTGCATTTGCGCTTCGCTAAGATTATCGGCGGGGGAAAAAAAACCGGCAGAATTTCCGCGGAAAgg                                                                                                                        >  2:794851/1‑151 (MQ=255)
                              gCGTAAAGTAGCGGATGCGCACATCGCTGTCGTCGCTGTGCAGCAGAAAAGCAGTTTCCGAATGGCCTAACTCGCGGGCGATAAGCTGCATTTGCGCTTCGCTAAGATTATCGGCGGGGAAAACAACACCGGCAGAATTGCCGCGAAACgg                                                                                                                        <  2:922080/151‑1 (MQ=255)
                                gTAAAGTAGCGGATGCGCACATCGCTGTCGTCGCTGTGCAGCAGAAAAGCAGTTTCCGAATGGCCTAACTCGCGGGCGATAAGCTGCATTTGCGCTTCGCTAAGATTATCGGCGGGGGAAAAAAAACCCGCAGAATTGGCGCGGAACgg                                                                                                                        >  1:593181/1‑149 (MQ=255)
                                   aaGTAGCGGATGCGCACATCGCTGTCGTCGCTGTGCAGCAGAAAAGCAGTTTCCGAATGGCCTAACTCGCGGGCGATAAGCTGCATTTGCGCTTCGCTAAGATTATCGGCGGGGGAAAAAAAACCCGCCGAAATGCCGCGAAACAGTtg                                                                                                                     >  2:1049511/1‑149 (MQ=255)
                                   aaGTAGCGGATGCGCACATCGCTGTCGTCGCTGTGCAGCAGAAAAGCAGTTTCCGAATGGCCTAACTCGCGGGCGATAAGCTGCATTTGCGCTTCGCTAAGATTATCGGCGGGGGAAAAAAAAACCGCAGGATTTTCGCGGAACgggt                                                                                                                      >  2:939681/1‑146 (MQ=255)
                                   aaGTAGCGGATGCGCACATCGCTGTCGTCGCTGTGCAGCAGAAAAGCAGTTTCCGAATGGCCTAACTCGCGGGCGATAAGCTGCATTTGCGCTTCGCTAAGATTATCGGCGGGGAAAACAACACCGGCAGAATTGCCGCGAAACGGTtgtg                                                                                                                   <  2:846698/151‑1 (MQ=255)
                                      tAGCGGATGCGCACATCGCTGTCGTCGCTGTGCAGCAGAAAAGCAGTTTCCGAATGGCCTAACTCGCGGGCGATAAGCTGCATTTGCGCTTCGCTAAGATTATCGGCGGGGGAAAAAACAACGGCAGAATTTTCGCGCAAAGGGTGTgggg                                                                                                                >  1:961513/1‑151 (MQ=255)
                                         cGGATGCGCACATCGCTGTCGTCGCTGTGCAGCAGAAAAGCAGTTTCCGAATGGCCTAACTCGCGGGCGATAAGCTGCATTTGCGCTTCGCTAAGATTATCGGCGGGGAAAACAACACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTaa                                                                                                             <  1:19245/151‑1 (MQ=255)
                                          ggATGCGCACATCGCTGTCGTCGCTGTGCAGCAGAAAAGCAGTTTCCGAATGGCCTAACTCGCGGGCGATAAGCTGCATTTGCGCTTCGCTAAGATTATCGGCGGGGGAAAAAAAAACGGCAGAAATGTCGCGGAACGGGtgggggggaa                                                                                                             >  2:98772/1‑142 (MQ=255)
                                          ggATGCGCACATCGCTGTCGTCGCTGTGCAGCAGAAAAGCAGTTTCCGAATGGCCTAACTCGCGGGCGATAAGCTGCATTTGCGCTTCGCTAAGATTATCGGCGGGGGAAAAAAAAACCGCCGGATTGCCGCGGAAACGGtgtgggg                                                                                                                 >  2:960245/1‑141 (MQ=255)
                                          ggATGCGCACATCGCTGTCGTCGCTGTGCAGCAGAAAAGCAGTTTCCGAATGGCCTAACTCGCGGGCGATAAGCTGCATTTGCGCTTCGCTAAGATTATCGGCGGGGAAAACAACACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTaaa                                                                                                            <  1:603045/151‑1 (MQ=255)
                                            aTGCGCACATCGCTGTCGTCGCTGTGCAGCAGAAAAGCAGTTTCCGAATGGCCTAACTCGCGGGCGATAAGCTGCATTTGCGCTTCGCTAAGATTATCGGCGGGGAAAACAACACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAg                                                                                                          <  1:443541/151‑1 (MQ=255)
                                                gcACATCGCTGTCGTCGCTGTGCAGCAGAAAAGCAGTTTCCGAATGGCCTAACTCGCGGGCGATAAGCTGCATTTGCGCTTCGCTAAGATTATCGGCGGGGAAAACAACACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATc                                                                                                      <  1:1103605/151‑1 (MQ=255)
                                                  acaTCGCTGTCGTCGCTGTGCAGCAGAAAAGCAGTTTCCGAATGGCCTAACTCGCGGGCGATAAGCTGCATTTGCGCTTCGCTAAGATTATCGGCGGGGAAAACAACACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGa                                                                                                    <  2:129036/151‑1 (MQ=255)
                                                         tGTCGTCGCTGTGCAGCAGAAAAGCAGTTTCCGAATGGCCTAACTCGCGGGCGATAAGCTGCATTTGCGCTTCGCTAAGATTATCGGCGGGGGAAACAAAACCCGCAGAAATGGCGCGGAAAGGGTGTGAGGGAAAAACATCGACgggg                                                                                               >  1:857597/1‑149 (MQ=255)
                                                          gtcgtcGCTGTGCAGCAGAAAAGCAGTTTCCGAATGGCCTAACTCGCGGGCGATAAGCTGCATTTGCGCTTCGCTAAGATTATCGGCGGGGAAAACAACACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTac                                                                                            <  2:732652/151‑1 (MQ=255)
                                                           tcgtcgCTGTGCAGCAGAAAAGCAGTTTCCGAATGGCCTAACTCGCGGGCGATAAGCTGCATTTGCGCTTCGCTAAGATTATCGGCGGGGGAAAAAAAACCGGCAGAAATTACGCGAAAAGGCTGTggggggaaaaaaacga                                                                                                    >  2:448884/1‑130 (MQ=255)
                                                                  tgtgCAGCAGAAAAGCAGTTTCCGAATGGCCTAACTCGCGGGCGATAAGCTGCATTTGCGCTTCGCTAAGATTATCGGCGGGGGAAAAAAAACCCGCAGAATTGCCGCGGAAAGGGTGTGGGGGGAAAGAAACCACGGGGGACACCCGCgg                                                                                    >  1:1017703/1‑151 (MQ=255)
                                                                         cagAAAAGCAGTTTCCGAATGGCCTAACTCGCGGGCGATAAGCTGCATTTGCGCTTCGCTAAGATTATCGGCGGGGGAAAAAACACCCGCAGAATTGTCCCGAAACGGGTGTGGGGGAAAAGCAACCACGTGGGACACCTCCGGttttct                                                                              >  1:523642/1‑147 (MQ=255)
                                                                          agAAAAGCAGTTTCCGAATGGCCTAACTCGCGGGCGATAAGCTGCATTTGCGCTTCGCTAAGATTATCGGCGGGGAAAACAACACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTa                                                                            <  1:79766/151‑1 (MQ=255)
                                                                            aaaaGCAGTTTCCGAATGGCCTAACTCGCGGGCGATAAGCTGCATTTGCGCTTCGCTAAGATTATCGGCGGGGGAAAAAAAAACGGCAGAATTGCCGCGAAAAGGGTGTGAGGGAAAAAAAACGACGTGGGACACCCGCGGGTTCAtt                                                                             >  2:355698/1‑148 (MQ=255)
                                                                                  aGTTTCCGAATGGCCTAACTCGCGGGCGATAAGCTGCATTTGCGCTTCGCTAAGATTATCGGCGGGGGAAAAAAAACCCGCAGAAATGCCGCCGAAAGGGTGTGGGGGAAAAAAAAAGAAGAGGGACACCTGCGGGTTCTTTAAAGtt                                                                       >  2:1089747/1‑148 (MQ=255)
                                                                                       ccGAATGGCCTAACTCGCGGGCGATAAGCTGCATTTGCGCTTCGCTAAGATTATCGGCGGGGGAAAAAACAACGGCCGAAATGCCGCGCAACGGGTGTGGGGGAAAAGAAACGACGTGGGACACCCGCGGGTTCATTAACGTTCTCCtgt                                                                >  1:533840/1‑150 (MQ=255)
                                                                                       ccGAATGGCCTAACTCGCGGGCGATAAGCTGCATTTGCGCTTCGCTAAGATTATCGGCGGGGGAAAAAACAACCGCAGAAATTCCGCGAAAAGGGTGTGAGGGAAAAACAACGACGGGGGACACCCGCGGGTTTATTAAAGTTCTcc                                                                   >  1:1076670/1‑147 (MQ=255)
                                                                                          aaTGGCCTAACTCGCGGGCGATAAGCTGCATTTGCGCTTCGCTAAGATTATCGGCGGGGGAAAAAAAAACGGCAGAAATGCCGCCGAAAGGGTGTGGGGGGAAAACATCGACGTGGGAAACCCGCGGGTTTATTTAAGTTCTCCTGTGACt                                                            >  1:450692/1‑151 (MQ=255)
                                                                                             ggCCTAACTCGCGGGCGATAAGCTGCATTTGCGCTTCGCTAAGATTATCGGCGGGGAAAACAACACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGACTgg                                                          <  1:939825/150‑1 (MQ=255)
                                                                                                  aaCTCGCGGGCGATAAGCTGCATTTGCGCTTCGCTAAGATTATCGGCGGGGGAAAAAAAACCCGCAGGAATGCCGCGGAAAGGGTGGGGGGGAAAAAAAACGACGTGGGACACCCGCGGGTTTATTTAAGTTTTCCttttgctgggg                                                        >  1:780770/1‑137 (MQ=255)
                                                                                                  aaCTCGCGGGCGATAAGCTGCATTTGCGCTTCGCTAAGATTATCGGCGGGGAAAACAACACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGACTGGAGAAc                                                     <  1:742303/150‑1 (MQ=255)
                                                                                                   aCTCGCGGGCGATAAGCTGCATTTGCGCTTCGCTAAGATTATCGGCGGGGGAAAAAAAACCCGCAGAATTGCCGCGGAAAGGGTGTGGGGGAAAAACAACGCCGCGGGACACCCGCGGGTTTTTTAAAATTCTCCTGTTGCTGGGGaa                                                      >  1:180177/1‑148 (MQ=255)
                                                                                                   aCTCGCGGGCGATAAGCTGCATTTGCGCTTCGCTAAGATTATCGGCGGGGGAAAAAAAAACCGCAGAAATGCCGCGGAACGGGTGTGGGGGAAAAACAACGACGGGGGACACCCCCGGGTTTCTTAAAATTCTCCTGTGACTGGGGaa                                                      >  1:447480/1‑148 (MQ=255)
                                                                                                      cgcgGGCGATAAGCTGCATTTGCGCTTCGCTAAGATTATCGGCGGGGAAAACAACACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGACTGGAGAACTAt                                                  <  1:877641/149‑1 (MQ=255)
                                                                                                       gcgGGCGATAAGCTGCATTTGCGCTTCGCTAAGATTATCGGCGGGGAAAACAACACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGACTGGAGAACTATCAt                                               <  1:770680/151‑1 (MQ=255)
                                                                                                             gATAAGCTGCATTTGCGCTTCGCTAAGATTATCGGCGGGGAAAACAACACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGACTGGAGAACTATCATAGCCt                                          <  2:257703/150‑1 (MQ=255)
                                                                                                             gATAAGCTGCATTTGCGCTTCGCTAAGATTATCGGCGGGGAAAACAACACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGACTGGAGAACTATCATAGCCTg                                         <  1:897207/151‑1 (MQ=255)
                                                                                                                aaGCTGCATTTGCGCTTCGCTAAGATTATCGGCGGGGAAAACAACACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGACTGGAGAACTATCATAGCCTGCaa                                     <  2:582295/151‑1 (MQ=255)
                                                                                                                        tttGCGCTTCGCTAAGATTATCGGCGGGGGGAACAAAACCCGCAGGAATTCCGCGGAACCGGTGTGAGGTGAAAAAAATTACGCGGGAAATCTGCGGTTTTGTTAAAGTTCTCCCGTGATTCGGGGAATATCATAGCCAGCCAAGTGGcgg                              >  2:335081/1‑149 (MQ=255)
                                                                                                                           gcgcTTCGCTAAGATTATCGGCGGGGAAAACAACACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGACTGGAGAACTATCATAGCCTGCAAGTGGCCGgag                           <  1:724770/150‑1 (MQ=255)
                                                                                                                                 cGCTAAGATTATCGGCGGGGGGAAAAACAACGGGAGAATTGGCGCCGAAAGGGTGTGTGGGAAAAAAAACCAAGGGGTAAACCTTCGGGTTTATTAACAATGTCCTGCGACTCGGGAAGTATCATACACTGCAAGGGGTCgg                             >  2:413383/1‑142 (MQ=255)
                                                                                                                                     aaGATTATCGGCGGGGAAAACAACACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGACTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCt                <  1:754874/151‑1 (MQ=255)
                                                                                                                                         ttATCGGCGGGGAAAACAACACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGACTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATc             <  2:557345/150‑1 (MQ=255)
                                                                                                                                           aTCGGCGGGGAAAACAACACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGACTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCgg          <  2:716411/151‑1 (MQ=255)
                                                                                                                                             cggcggGGAAAACAACACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGACTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGcc        <  1:1074162/151‑1 (MQ=255)
                                                                                                                                                cggGGAAAACAACACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGACTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAgg     <  1:559130/151‑1 (MQ=255)
                                                                                                                                                   ggAAAACAACACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGACTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTg  <  1:1085988/151‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                       
CGTGACCGCAAATCGGCACTTCAACCGTTGGCGTAAAGTAGCGGATGCGCACATCGCTGTCGTCGCTGTGCAGCAGAAAAGCAGTTTCCGAATGGCCTAACTCGCGGGCGATAAGCTGCATTTGCGCTTCGCTAAGATTATCGGCGGGGAAAACAACACCGGCAGAATTGCCGCGAAACGGTTGTGAGGTAAAAGCATCGACGTGGTACACCTGCGGTTTCATTAACGTTCTCCTGTGACTGGAGAACTATCATAGCCTGCAAGTGGCCGGAGAGCGAAGGGCTATCCGGCCAGGGTG  >  NC_000913/1535636‑1535933

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: