Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,252,646	0	T	G	41.2%	127.0 / 13.3	68	V316G (GTA→GGA)	yeiI	putative kinase
Reads supporting (aligned to +/- strand):  ref base T (6/34);  new base G (28/0);  total (34/34)
Fisher's exact test for biased strand distribution p-value = 2.70e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGCAAGGTGTTCAGCAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTC‑TTGAAGGATACAGTTTCCGCGAC‑AGCG‑CCCGTTTTGCGGTAGCCTGCG‑C‑GGCAATTTCGCGCGCCAGCGGCAG‑CTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTT  >  NC_000913/2252498‑2252795                                                                                                                                                     |                                                                                                                                                            cAGCAAGGTGTTCAGCAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGta                                                                                                                                                            <  1:403847/150‑1 (MQ=255)   gCAAGGTGTTCAGCAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGtatat                                                                                                                                                         <  1:87347/151‑1 (MQ=255)       ggTGTTCAGCAGCTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCTCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCt                                                                                                                                                     <  2:319918/151‑1 (MQ=255)       ggTGTTCAGCAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCt                                                                                                                                                     <  1:204423/151‑1 (MQ=255)             cAGCAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTC‑tt                                                                                                                                               <  1:965598/150‑1 (MQ=255)             cAGCAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGATATAGTTTTC‑ttt                                                                                                                                              >  2:485423/1‑150 (MQ=255)               gCAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCGCGGTATATAGCtttt‑tt                                                                                                                                               >  2:540111/1‑148 (MQ=255)               gCAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTC‑TTGaa                                                                                                                                            <  2:919797/151‑1 (MQ=255)                cAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCTGGGATATAGAtttt‑ttttt                                                                                                                                            >  2:788304/1‑147 (MQ=255)                            tATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTC‑TTGAAGGATACAGTTTc                                                                                                                                <  2:421588/150‑1 (MQ=255)                                tGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTC‑TTGAAGGATACAGTTTCcgcg                                                                                                                            <  2:44995/150‑1 (MQ=255)                                 gCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTC‑TTGAAGGATACAGTTTCCGCGAc                                                                                                                          <  2:450407/151‑1 (MQ=255)                                  cccGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGATATAGCTTTT‑TTTTAGGGTACAGGTTTcgc                                                                                                                             >  1:390129/1‑147 (MQ=255)                                       tGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGATATAGATTTC‑TTTTAAGGTAAAGTTTCCGCGAC‑CGCG‑cc                                                                                                                  >  1:22590/1‑151 (MQ=255)                                         aGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGATATAGCTTTT‑TTTTAGGATAAAGTTTTCGCGGC‑CGCG‑ccc                                                                                                                 >  2:403117/1‑150 (MQ=255)                                             aGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCCGGGATATAGCTTTT‑TTTTAGGATAAAGATTTCGCGGC‑AGGG‑CCCGcttt                                                                                                            >  2:1085559/1‑151 (MQ=255)                                             aGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGGATATAGATTTT‑TTTTAGGATACAGTTTCCCCGGC‑AGCG‑CCCGgttt                                                                                                            >  1:746560/1‑151 (MQ=255)                                                  aTTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGATATAACTTTC‑TTTTAGGATACAGATTTCGCGAC‑AGCG‑CCCCCTTTGCgg                                                                                                        >  1:324633/1‑150 (MQ=255)                                                   ttGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGGTATAGCTTTT‑TTTTAGGGGAAAGGTTTCCCGGC‑AGCG‑CCCCGTTTGCGGtg                                                                                                      >  2:863499/1‑150 (MQ=255)                                                    tGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTT‑TTTAAGGATACCAGTTCCCCGAC‑AACGCCCCGtttttggtgg                                                                                                      >  1:361374/1‑145 (MQ=255)                                                      cAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTGTATTACTTTC‑TTTGAGGGTAAAGTTTCCTCGACAACCG‑CCCG‑TTTGCGGGAGcc                                                                                                   >  1:735987/1‑151 (MQ=255)                                                       aGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTTGGATATAACTTTT‑TTTAAGGGTACAGATTCTGCGAC‑AGCG‑CCCGCTTTTCGGTAGcc                                                                                                   >  2:218004/1‑150 (MQ=255)                                                       aGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTC‑TTGAAGGATACAGTTTCCGCGAC‑AGCG‑CCCGTTTTGCGGTAGCCt                                                                                                  <  2:579975/151‑1 (MQ=255)                                                           aaaaGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCCGGGATATAGCTTTT‑TTTTAGGATAAAGTTTCCCCGAC‑AGCG‑CCCGCTTTTTGGGAGCCCgcg‑c                                                                                             >  1:587545/1‑151 (MQ=255)                                                           aaaaGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGATATAGATTTT‑TTTATGGATACAGTTTCCCCGAC‑AGCG‑CCCGCCTTTCGGtttgc                                                                                                   >  2:407413/1‑142 (MQ=255)                                                               ggATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGGGTATAGCTTTT‑TTGAAGGATAAAAGTTTCGCGGC‑AGCG‑CCCGGTTTTCGGGAGCCTgcg‑c                                                                                             >  1:253502/1‑147 (MQ=255)                                                               ggATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGGATATAGCTTTT‑TTTTAGGATAAAGTTTTTCCGCC‑AGCG‑CCCCCTTTGCGGTGGcccttgcg                                                                                             >  2:726786/1‑142 (MQ=255)                                                                gATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTC‑TTGAAGGATACAGTTTCCGCGAC‑AGCG‑CCCGTTTTGCGGTAGCCTGCG‑C‑GGCaa                                                                                       <  2:571491/151‑1 (MQ=255)                                                                  tGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCCGGGATAATACTTTT‑TTTAAGGGGAAAAGTTCCGCGGC‑AGCG‑CCCCCGTTTCGGTAGCCCTCG‑C‑CGCGAtt                                                                                     >  2:536056/1‑151 (MQ=255)                                                                  tGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTC‑TTGAAGGATACAGTTTCCGCGAC‑AGCG‑CCCGTTTTGCGGTAGCCTGCG‑C‑GGCAAt                                                                                      <  2:66984/150‑1 (MQ=255)                                                                    gagaACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGATATAGCTTTT‑TTTAAGGATACAGATTTCGCGAC‑AGCG‑CCCCCTTTTCGGGAGCCTGCG‑C‑GGCAAtttt                                                                                   >  1:105830/1‑150 (MQ=255)                                                                     agaACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTC‑TTGAAGGATACAGTTTCCGCGAC‑AGCG‑CCCGTTTTGCGGTAGCCTGCG‑C‑GGCAATTTcg                                                                                  <  2:249349/151‑1 (MQ=255)                                                                     agaACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGGGTATAGCTTTT‑TTTGTGGGGACAGTTTTCGCGGC‑AGCG‑CCCCCTTTTGGGTAGCCCGCG‑C‑GGCGAttt                                                                                    >  2:305754/1‑149 (MQ=255)                                                                        aCAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGGGTTTAACTTTC‑TTGAAAGGTACCGTTTTCGCGCC‑AGCG‑CCCCCTTTTCGGTATGCCGCG‑C‑GGGCAtttacc                                                                                 >  1:1102429/1‑146 (MQ=255)                                                                               ttGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTC‑TTGAAGGATACAGTTTCCGCGAC‑AGCG‑CCCGTTTTGCGGTAGCCTGCG‑C‑GGCAATTTCGCGCGCCAGc                                                                         <  2:539840/150‑1 (MQ=255)                                                                                tGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTC‑TTGAAGGATACAGTTTCCGCGAC‑AGCG‑CCCGTTTTGCGGTAGCCTGCG‑C‑GGCAATTTCGCGCGCCAGCg                                                                        <  1:798780/150‑1 (MQ=255)                                                                                   tGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGATTTAACTTTT‑TTTTAGGATACACATTTCGGGAC‑AGAG‑CCCGGTTTGCCGTAGCCCGCGCC‑GGAAATTTccgccgc                                                                             >  2:1117166/1‑137 (MQ=255)                                                                                    gACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTC‑TTGAAGGATACAGTTTCCGCGAC‑AGCG‑CCCGTTTTGCGGTAGCCTGCG‑C‑GGCAATTTCGCGCGCCAGCGGCa                                                                     <  2:318239/149‑1 (MQ=255)                                                                                    gACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTC‑TTGAAGGATACAGTTTCCGCGAC‑AGCG‑CCCGTTTTGCGGTAGCCTGCG‑C‑GGCAATTTCGCGCGCCAGCGGCAG‑c                                                                  <  2:239238/151‑1 (MQ=255)                                                                                    gACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTC‑TTGAAGGATACAGTTTCCGCGAC‑AGCG‑CCCGTTTTGCGGTAGCCTGCG‑C‑GGCAATTTCGCGCGCCAGCGGCAG‑c                                                                  <  2:357985/151‑1 (MQ=255)                                                                                          gcCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTC‑TTGAAGGATACAGTTTCCGCGAC‑AGCG‑CCCGTTTTGCGGTAGCCTGCG‑C‑GGCAATTTCGCGCGCCAGCGGCAG‑CTTAaac                                                            <  1:619977/151‑1 (MQ=255)                                                                                            cAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTGTATAGCTTTT‑TTTAAGGGTAAAGTTTTCGCGAC‑CGCG‑CCCGTTTTTCGGGAGCCTGCG‑C‑GGCAATTTTGCGTGCCAGCGGCGG‑CTTtaaaa                                                           >  1:870091/1‑148 (MQ=255)                                                                                             aGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGATATAGCTTTT‑TTTTAGGGTACAGTTTTCCCGCC‑AGCG‑CCCCCTTTTTGGGAGCCTGCG‑C‑GGCAATTTTGCCCGCCCGCGGCAG‑CTTTaaaaa                                                          >  1:489116/1‑150 (MQ=255)                                                                                                gcATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGGATATAGCTTTCTTTGAAGG‑TAAAGTTTCCGCGAC‑AGCG‑CCCGGTTTTCGGGAGCCTGCG‑C‑GGCAAATTTTCGCGCCAGCGGCCG‑GTTTAAAAaccc                                                       >  1:1022498/1‑150 (MQ=255)                                                                                                gcATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGATATAAATTTT‑TTTAAGGATAAAGGTTTCGCCAC‑AGCC‑CCCCTTTTTCGGTAGGCCGCG‑C‑GGCCATTTTTCGCGCCCGCCGCCG‑CTTAAAAAaccc                                                       >  1:655905/1‑150 (MQ=255)                                                                                                 cATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCCGGGATATAATTTTT‑TTTAAGGGTAAAGTTTTCGCGAC‑AGAG‑CCCCCTTTTCGGGAGCCCGCG‑C‑GGCGATTTTGCGCGCCCGCGGCAG‑CTTAAAAAaacc                                                       >  2:895420/1‑149 (MQ=255)                                                                                                  aTACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCCGGGATATAAGTTTT‑TTGGAGGGTAAAATTTTCGCGAC‑AACG‑CCCGCTTTTCGGGTGGCCGCG‑C‑GGGCATTTTTCGCGCCCGCGGGGGCCTTAAAAAaccc                                                       >  2:213444/1‑149 (MQ=255)                                                                                                  aTACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCCGGGATATAAATTTT‑TTTTAGGATACAGTTTTTTCCAC‑AGCG‑CCCCGGTTTCGTTTGCCGGCG‑CGGGCAATTT‑GCGCGCCCGCGGCCG‑GTTAAAAAacccc                                                      >  2:259625/1‑148 (MQ=255)                                                                                                  aTACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGATATAGATTTT‑TTTAAGGATACAATTTCCGCCAC‑AGCG‑CCCGCTTTTCGGGAGCCTGCC‑C‑GGGCATTTTCCGCGCCCGCGGCCG‑CTTAAACAaacc                                                       >  1:1030355/1‑148 (MQ=255)                                                                                                   tACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCTTGGATATATCTTTT‑TTTTAGGATACAGATTTCGCGCC‑AACG‑CCCCCTTTTCTGTAGCCTGTG‑C‑GGCAAATTTTTGCGCCCCCGGCCG‑CTTATACAaacccacc                                                   >  2:375420/1‑151 (MQ=255)                                                                                                     cGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTC‑TTTAAGGATACAGTTTTCCCGGC‑AGCG‑CCCGCTTTTCGGGAGCCCGCG‑CGGGCAATTT‑GCGCGCCCCCGGCCGCCTTAAAAAacccaccc                                                   >  1:691118/1‑146 (MQ=255)                                                                                                         aGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTC‑TTGAAGGATACAGTTTCCGCGAC‑AGCG‑CCCGTTTTGCGGTAGCCTGCG‑C‑GGCAATTTCGCGCGCCAGCGGCAG‑CTTAAACAACCCTACCCTGTCt                                             <  2:905989/151‑1 (MQ=255)                                                                                                         aGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTC‑TTGAAGGATACAGTTTCCGCGAC‑AGCG‑CCCGTTTTGCGGTAGCCTGCG‑C‑GGCAATTTCGCGCGCCAGCGGCAG‑CTTAAACAACCCTACCCTGTCt                                             <  1:166467/151‑1 (MQ=255)                                                                                                            aGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTC‑TTGAAGGATACAGTTTCCGCGAC‑AGCG‑CCCGTTTTGCGGTAGCCTGCG‑C‑GGCAATTTCGCGCGCCAGCGGCAG‑CTTAAACAACCCTACCCTGTCTGcc                                          <  2:214706/151‑1 (MQ=255)                                                                                                              aCAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTC‑TTGAAGGATACAGTTTCCGCGAC‑AGCG‑CCCGTTTTGCGGTAGCCTGCG‑C‑GGCAATTTCGCGCGCCAGCGGCAG‑CTTAAACAACCCTACCCTGTCTGCCGa                                        <  1:694971/151‑1 (MQ=255)                                                                                                              aCAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTC‑TTGAAGGATACAGTTTCCGCGAC‑AGCG‑CCCGTTTTGCGGTAGCCTGCG‑C‑GGCAATTTCGCGCGCCAGCGGCAG‑CTTAAACAACCCTACCCTGTCTGCCGa                                        <  1:435070/151‑1 (MQ=255)                                                                                                                aGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTC‑TTGAAGGATACAGTTTCCGCGAC‑AGCG‑CCCGTTTTGCGGTAGCCTGCG‑C‑GGCAATTTCGCGCGCCAGCGGCAG‑CTTAAACAACCCTACCCTGTCTGCCGATa                                      <  1:76627/151‑1 (MQ=255)                                                                                                                   tttGGTGCTGACGATGGTTTTATGGCGGGCCCGGGATATAGCTTTT‑TTTTAGGATAAAGGTTTCGCGAC‑AGCG‑CCCCCTTTTCGGGAGCCCGCG‑C‑GGCCAATTTGCGCGCCCGCGGCAG‑CCTTAAAAACCCCACCCCGTCTGCCGATaa                                     >  1:983696/1‑149 (MQ=255)                                                                                                                            gACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTC‑TTGAAGGATACAGTTTCCGCGAC‑AGCG‑CCCGTTTTGCGGTAGCCTGCG‑C‑GGCAATTTCGCGCGCCAGCGGCAG‑CTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTc                            <  2:1107716/149‑1 (MQ=255)                                                                                                                                  ggTTTTATGGCGGGCCTGGTATATAGCTTTC‑TTGAAGGATACAGTTTCCGCGAC‑AGCG‑CCCGTTTTGCGGTAGCCTGCG‑C‑GGCAATTTCGCGCGCCAGCGGCAG‑CTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTg                     <  1:226588/150‑1 (MQ=255)                                                                                                                                      ttATGGCGGGCCTGGTATATAGCTTTC‑TTGAAGGATACAGTTTCCGCGAC‑AGCG‑CCCGTTTTGCGGTAGCCTGCG‑C‑GGCAATTTCGCGCGCCAGCGGCAG‑CTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCaa                 <  2:524213/150‑1 (MQ=255)                                                                                                                                       tATGGCGGGCCTGGTATATAGCTTTC‑TTGAAGGATACAGTTTCCGCGAC‑AGCG‑CCCGTTTTGCGGTAGCCTGCG‑C‑GGCAATTTCGCGCGCCAGCGGCAG‑CTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAAt                <  1:216573/150‑1 (MQ=255)                                                                                                                                        aTGGCGGGCCTGGTATATAGCTTTC‑TTGAAGGATACAGTTTCCGCGAC‑AGCG‑CCCGTTTTGCGGTAGCCTGCG‑C‑GGCAATTTCGCGCGCCAGCGGCAG‑CTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATgg              <  2:439809/151‑1 (MQ=255)                                                                                                                                        aTGGCGGGCCTGGTATATAGCTTTC‑TTGAAGGATACAGTTTCCGCGAC‑AGCG‑CCCGTTTTGCGGTAGCCTGCG‑C‑GGCAATTTCGCGCGCCAGCGGCAG‑CTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATgg              <  2:822648/151‑1 (MQ=255)                                                                                                                                        aTGGCGGGCCTGGTATATAGCTTTC‑TTGAAGGATACAGTTTCCGCGAC‑AGCG‑CCCGTTTTGCGGTAGCCTGCG‑C‑GGCAATTTCGCGCGCCAGCGGCAG‑CTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATg               <  1:895198/150‑1 (MQ=255)                                                                                                                                          ggCGGGCCTGGTATATAGCTTTC‑TTGAAGGATACAGTTTCCGCGAC‑AGCG‑CCCGTTTTGCGGTAGCCTGCG‑C‑GGCAATTTCGCGCGCCAGCGGCAG‑CTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGgtg            <  2:669888/151‑1 (MQ=255)                                                                                                                                          ggCGGGCCTGGTATATAGCTTTC‑TTGAAGGATACAGTTTCCGCGAC‑AGCG‑CCCGTTTTGCGGTAGCCTGCG‑C‑GGCAATTTCGCGCGCCAGCGGCAG‑CTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGgt             <  1:470821/150‑1 (MQ=255)                                                                                                                                             gggCCTGGTATATAGCTTTC‑TTGAAGGATACAGTTTCCGCGAC‑AGCG‑CCCGTTTTGCGGTAGCCTGCG‑C‑GGCAATTTCGCGCGCCAGCGGCAG‑CTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTaa         <  1:470973/151‑1 (MQ=255)                                                                                                                                             gggCCTGGTATATAGCTTTC‑TTGAAGGATACAGTTTCCGCGAC‑AGCG‑CCCGTTTTGCGGTAGCCTGCG‑C‑GGCAATTTCGCGCGCCAGCGGCAG‑CTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTa          >  2:681042/1‑150 (MQ=255)                                                                                                                                                    gTATATAGCTTTC‑TTGAAGGATACAGTTTCCGCGAC‑AGCG‑CCCGTTTTGCGGTAGCCTGCG‑C‑GGCAATTTCGCGCGCCAGCGGCAG‑CTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGtt  <  1:1079729/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                            CAGCAAGGTGTTCAGCAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTTC‑TTGAAGGATACAGTTTCCGCGAC‑AGCG‑CCCGTTTTGCGGTAGCCTGCG‑C‑GGCAATTTCGCGCGCCAGCGGCAG‑CTTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTT  >  NC_000913/2252498‑2252795

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑