Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,326,450	0	T	G	51.0%	82.6 / 100.4	51	G114G (GGT→GGG)	atoB	acetyl‑CoA acetyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/25);  new base G (26/0);  total (26/25)
Fisher's exact test for biased strand distribution p-value = 4.03e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.51e-12

CGTCAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATGGG  >  NC_000913/2326307‑2326584                                                                                                                                                |                                                                                                                                       cgTCAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGaaa                                                                                                                                 <  1:912772/151‑1 (MQ=255)     aGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAatat                                                                                                                             <  1:1093994/151‑1 (MQ=255)        cACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGAAAATATg                                                                                                                            >  1:946655/1‑149 (MQ=255)            gTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGtt                                                                                                                        <  1:589464/149‑1 (MQ=255)            gTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTa                                                                                                                      <  1:146073/151‑1 (MQ=255)            gTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTa                                                                                                                      <  1:406447/151‑1 (MQ=255)               aaaaaGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAATATGAGGTTAGcc                                                                                                                   >  1:1046130/1‑151 (MQ=255)                aaaaGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGccc                                                                                                                  <  1:581968/151‑1 (MQ=255)                aaaaGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGTTGGGAAAAATGAGGTTTGccc                                                                                                                  >  2:372880/1‑151 (MQ=255)                     cGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAATATGAGTTTAGCCCCCtac                                                                                                             >  1:379468/1‑151 (MQ=255)                           ggCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCtacttact                                                                                                        <  2:964532/150‑1 (MQ=255)                                  aCGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAATATGAGTTTAGCCCCCCACTTACTCGATGc                                                                                                  >  2:270148/1‑149 (MQ=255)                                  aCGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAAAATGAGGTTAGCCCCCcactt                                                                                                           >  1:956398/1‑140 (MQ=255)                                   cGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGGTGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCaa                                                                                                >  2:802297/1‑150 (MQ=255)                                    ggTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCaaaa                                                                                              <  1:807822/151‑1 (MQ=255)                                           ggATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGAAAAAATGAGTTTAGCCCCCCACTTACTCGATGCcaaaac                                                                                            >  1:835732/1‑144 (MQ=255)                                               tCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGGTGGGAAAAATGAGGTTAGCCCCCCACTTACTCGATGCAAAAGCACGCTCTgg                                                                                   >  2:210929/1‑151 (MQ=255)                                               tCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGAAAATATGAGTTTTGCCCCCTACTTACTCGATGCAAAAACACGCCCTg                                                                                    >  1:921838/1‑150 (MQ=255)                                                 aCGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGtt                                                                                 <  2:339101/151‑1 (MQ=255)                                                    gTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGTAAAATTGAGGGTATCGCCCTACTTACTTGTTGCAAAAGCACGCTCTgggttt                                                                               >  1:308153/1‑146 (MQ=255)                                                        aTAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAAAATGAGTTTAGCCCCCTACTTACTCGATGGAAAAACACGCCCTGGTTATCGTc                                                                           >  1:1083905/1‑150 (MQ=255)                                                          aaaGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTg                                                                        <  1:305610/151‑1 (MQ=255)                                                          aaaGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTg                                                                        <  2:711877/151‑1 (MQ=255)                                                           aaGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAATATGAGTTTAGGCCCCTACTTACTCGATGCAAAAACACGCCCTGGTTATCGTCTTgg                                                                       >  2:519742/1‑151 (MQ=255)                                                             gTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGgag                                                                     <  2:373871/151‑1 (MQ=255)                                                             gTATGTGGTGCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGga                                                                      <  2:1000735/150‑1 (MQ=255)                                                               aTGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGgaga                                                                    <  1:551205/150‑1 (MQ=255)                                                               aTGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGAc                                                                   <  1:325723/151‑1 (MQ=255)                                                               aTGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGAc                                                                   <  1:270200/151‑1 (MQ=255)                                                                    gTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACa                                                              <  1:103117/151‑1 (MQ=255)                                                                     ttCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAATATGAGGTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACgg                                                                 >  1:526485/1‑147 (MQ=255)                                                                        gggTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGCAAAAATGAGGTTTACCCCCTACTTAACTGTGGCAAAAGCAAGGCCTGGTTTTCGTCTTTGCGACGGACCgggt                                                          >  1:236040/1‑149 (MQ=255)                                                                           tCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGAAAAAATGAGTTTAGCCCCCCACTTACTCGATGCAAAAACACGCTCTGGTTATCGTCTTTGGGACGGGCAGGgtt                                                         >  1:798720/1‑149 (MQ=255)                                                                              tAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGa                                                     <  1:648181/150‑1 (MQ=255)                                                                                 aaGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAATATGAGTTTAGCCCCCCACTTACTCGATGCAAAAGCACGCTCTGGGTATCGGCTTGGAGACGGACAGGTTTATGACGt                                                  >  1:1005983/1‑150 (MQ=255)                                                                                   gtgtGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAAAATGAGTTTTGCCCCCCACTTACTCCATGCAAAAGCACGCTCCGGTTATCGTCTTGGAGACGGACAGGGTTATGACGTaa                                                >  1:1078276/1‑150 (MQ=255)                                                                                      tgGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAATATTGGTTTAGCCCCCTACTTTATCGATGCAAAAGCACGCCCTGGTTATCGTCTTGGGGACGGACAGGGTTTTTACGGAAACCt                                            >  2:1117562/1‑151 (MQ=255)                                                                                      tgGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAATATGAGTTTTGCCCCCTACTTACTCCATGCAAAAGCACGCCCTGGGTTTTGTCTTGGGAACGGACAGGTTTTTGACGTAATcc                                             >  2:106774/1‑150 (MQ=255)                                                                                        gcgcTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTgc                                          <  2:893686/151‑1 (MQ=255)                                                                                          gcTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAATATGAGTTTTGCCCCCTACTTACTCGATGCAAAAGCACGCCCTGGTTATCGTCTTGGAGACGGACAGGGTTATGACGTAATCCTgcg                                         >  1:606991/1‑150 (MQ=255)                                                                                          gcTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAATATGAGGTTAGCCCCCCACTTACTCGATGCAAAAGCACGCTCTGGGTATCGTCTTGGAGACGGACAGGGTTATGACGTAATcc                                             >  1:984699/1‑146 (MQ=255)                                                                                          gcTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAAAATGAGTTTAGGCCCCTACTTACTCGGTGCAAAAACAAGCTCTGGGTATCGTTTTGGAGACGGGCCGGGTTTTGACGGAAACCTgcgc                                        >  2:1100698/1‑151 (MQ=255)                                                                                                   ccAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCGCTGGTTATCGTCTTGGAGACGGGAAGGGTTATGAAGTAATTCTGCGCGGTGGcc                                 >  1:639345/1‑149 (MQ=255)                                                                                                     aGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGAt                             <  1:1117723/151‑1 (MQ=255)                                                                                                                  aggTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCAt                <  2:824537/151‑1 (MQ=255)                                                                                                                   ggTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATg               <  2:60329/151‑1 (MQ=255)                                                                                                                       aGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTa           <  1:999552/151‑1 (MQ=255)                                                                                                                       aGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAATATGAGTTTTGCCCCCTACTTACTCGATGCAAAAACACGCTCTGGTTATCGTCTTGGGGACGGGCAGGGTTATGACGTAATCCTGCGCGGTGGCCTGATGTGCGCCACCCATgggt            >  1:387978/1‑148 (MQ=255)                                                                                                                        ggCGCAGAGCATTGTGGCGGGGGGGATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGGTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTAt          >  1:402941/1‑151 (MQ=255)                                                                                                                          cgcAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCa        <  1:802430/151‑1 (MQ=255)                                                                                                                                gCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATggg  <  1:42329/151‑1 (MQ=255)                                                                                                                                                |                                                                                                                                       CGTCAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATGGG  >  NC_000913/2326307‑2326584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑