Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,206,435	0	C	T	47.4%	46.4 / 66.2	76	intergenic (‑179/‑28)	ttdR/ttdA	transcriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (8/32);  new base T (36/0);  total (44/32)
Fisher's exact test for biased strand distribution p-value = 3.41e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAA‑GTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG  >  NC_000913/3206286‑3206585                                                                                                                                                        |                                                                                                                                                        aGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTcc                                                                                                                                                        <  1:813619/151‑1 (MQ=255)      aTTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTTCAGAc                                                                                                                                                    >  1:358973/1‑150 (MQ=255)      aTTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTCCAGACa                                                                                                                                                   <  1:617620/151‑1 (MQ=255)               aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTTCAGACAaaaaaaaa                                                                                                                                           >  1:232290/1‑150 (MQ=255)               aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTCCAGACAAATACaaaa                                                                                                                                          <  1:645199/151‑1 (MQ=255)                     gCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTTCCGACAAATACaaaaa                                                                                                                                         >  1:436005/1‑146 (MQ=255)                        aTTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTCCAGACAAATACAAAAACTGGAGtt                                                                                                                                 <  1:352268/151‑1 (MQ=255)                          tCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTCCAGACAAATACAAAAACTGGAGTTg                                                                                                                                <  1:293805/150‑1 (MQ=255)                            ccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑ATTTTTCCCACCAAAACAAAAAATgggggtggcc                                                                                                                             >  2:341301/1‑143 (MQ=255)                              tGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCCAAAAAAAAAAAAAAATTGGGGttccat                                                                                                                             >  2:209934/1‑146 (MQ=255)                               gCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTCCAGACAAATACAAAAACTGGAGTTGCCat                                                                                                                            <  2:1118044/149‑1 (MQ=255)                                  ctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑ATTTTTCACACAAAAAAAAAAAATgg                                                                                                                                     >  2:995083/1‑137 (MQ=255)                                   tGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTTCAGACAAAAACAAAAAATTGGGTTGCCatg                                                                                                                           >  1:947715/1‑146 (MQ=255)                                   tGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTTCAGAAAAAAAAAAAAAATGGAGTTGTCa                                                                                                                             >  2:342455/1‑144 (MQ=255)                                    ggCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGc                                                                                                                     <  1:464603/151‑1 (MQ=255)                                     gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTCCAGACAAATACAAAAACTGGAGTTGCCAtgatga                                                                                                                       <  1:784140/148‑1 (MQ=255)                                     gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCg                                                                                                                    <  2:53054/151‑1 (MQ=255)                                     gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCg                                                                                                                    <  1:970700/151‑1 (MQ=255)                                        cAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGaaa                                                                                                                 <  2:523075/151‑1 (MQ=255)                                        cAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TATTTTCCCACAAAAAAAAAAACATGAGTTGCCctggatt                                                                                                                       >  1:30301/1‑141 (MQ=255)                                          atatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAA‑Gt                                                                                                              <  1:781990/151‑1 (MQ=255)                                           tatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTTCAGAAAAATAAAAAAAATAGAGGTGCCATGATGAg                                                                                                                      >  1:913983/1‑143 (MQ=255)                                              tcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑ATTTTTCAGACAAAAAAAAAAAATGGAGGTGGCATGATGAGGGAAA‑Gtaa                                                                                                            >  1:68477/1‑149 (MQ=255)                                               cattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTTCAAACAAAAAAAAAAAATGGAGGTGGCCTGGTGAGGGGAA‑Gtaa                                                                                                            >  2:826807/1‑148 (MQ=255)                                                  tcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAA‑GTAATAAGc                                                                                                       <  2:793049/150‑1 (MQ=255)                                                   cTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGG‑AA‑ATTTTTTAAAAAAAAAAAAAAAAAGGAGGTGTCCTGATGGGAGAAA‑Ggaaaaa                                                                                                         >  2:324718/1‑147 (MQ=255)                                                    ttATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAA‑GTAATAAGc                                                                                                       <  2:579329/148‑1 (MQ=255)                                                    ttATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGG‑AA‑ATTTTCCTCCTCAAAACAAAAAATGGAGGTGGCATGATGAGCGAAA‑Gaaaaaaaaaa                                                                                                     >  1:374506/1‑146 (MQ=255)                                                     tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTTCTGACAAAAAAAAAAAATGGAGTTGCCATGGTGAGGGGAA‑GAAAAAAGaaa                                                                                                     >  2:247728/1‑149 (MQ=255)                                                       ttttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑GA‑TTTTTTCAGACAAAAACAAAAAATGGAGTTGTCATGATGAGCGAAA‑Agaataaaaaaaa                                                                                                   >  2:211117/1‑143 (MQ=255)                                                        tttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTTCAGAAAAATACAAAAAATGGGGTTGCCCTGATGAGCGGAA‑GGAATAAGGAACaag                                                                                                 >  1:1095217/1‑148 (MQ=255)                                                         ttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑GA‑TTTTTTCAGAAAAAAACAAAAAATGGAGTTGTCATGATGAGGGAAA‑Gtaataaaaaaaaag                                                                                                 >  2:355297/1‑141 (MQ=255)                                                          tCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAA‑GTAATAAGCAACAGGCa                                                                                               <  1:718026/150‑1 (MQ=255)                                                            gcgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTTCCGAAAAAAAAAAAAAATGGAGTTGCCATGATGAGGGAAA‑GGAATAAACAAAAGGCAggg                                                                                            >  2:1060431/1‑149 (MQ=255)                                                                tGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTTCAAACAAAAACAAAAAATTGAGTTGGCATGATGAGCGGAA‑GAAATAAACAACAAGCAGTGaa                                                                                          >  2:720874/1‑149 (MQ=255)                                                                     cACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAA‑GTAATAAGCAACAGGCAGTGAATAAGTTg                                                                                   <  2:149398/151‑1 (MQ=255)                                                                     cACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑ATTTTTCAAAAAAATACAAAAAATGGAGGTGTCATGATGAGGGAAA‑ATAATAAAGAAAAAGCAGAGAATAAAtt                                                                                    >  2:668402/1‑150 (MQ=255)                                                                      aCGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑GA‑TTTTTTCAAACAAAAACAAAAACTGGAGTTGTCATGATGAGGGGAA‑ATAAAAAGAAAAAGGCAGGGAATAAAtt                                                                                    >  2:627798/1‑149 (MQ=255)                                                                      aCGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAA‑GTAATAAGCAACAGGCAGTGAATAAGTTGa                                                                                  <  2:154502/151‑1 (MQ=255)                                                                        gAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAA‑GTAATAAGCAACAGGCAGTGAATAAGTTGACa                                                                                <  2:735450/151‑1 (MQ=255)                                                                        gAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑ATTTTTTAAACAAAAACAAAAAATCGGGTTGCGATGATGGGCGAAA‑AGAAAAAGGAAAAGGCAATGAATAAGtt                                                                                    >  1:355441/1‑147 (MQ=255)                                                                                aaTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTTCAGACAAATACAAAAAATGGAGTTGCCATGATGTGCGCAA‑AGAATAAACAAAAGGGAGGGAATAAGTTGACagag                                                                             >  1:349392/1‑146 (MQ=255)                                                                                 aTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑CA‑TTTTTTCATAAAAAAAAAAAAACTGGAGTTGCCATTACGAGGGCGA‑GGAAAAAGGAATAGGCCGTGAATTAGCTgcaacatattg                                                                         >  2:454753/1‑139 (MQ=255)                                                                                   tACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAA‑GTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCg                                                                      <  2:121277/150‑1 (MQ=255)                                                                                   tACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑ATTTTTTAGAACAAAAAAAAAACTGGAGGTTGCACGGTGAGGGAAAGGAAAAAAGAAAAAGGAAGGGAAAAAG‑TGACAAAGAATGTCg                                                                      >  2:324595/1‑150 (MQ=255)                                                                                     cATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTTCAGACAAATACAAAAAATGGAGTTGGCATGATGGGCGAAA‑ATAATAAACAACAGGCAGAGAAAAAAATGTCAGAGAtt                                                                          >  1:1092511/1‑144 (MQ=255)                                                                                     cATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑ATTTTTCCCACAAAAAAAAAAAATGGAGTTGGCATGGTTAGGGGAA‑GTAATAAACAACAAGCAGGGAAAAAAGTGACAGAGATTGTCGCt                                                                    >  1:291830/1‑150 (MQ=255)                                                                                          tCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTTCAGACAAATAAAAAAAATGGAGTTGCCATGATGAGCGAAA‑GTAATAAACAAAAGGGAGGGAATAAGTTGACAGAGATTGTCGCTAACttt                                                              >  1:609759/1‑151 (MQ=255)                                                                                             cGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTTCCAACAAAAAAAAAAAATGGGGTTGCCCTGATGAGCGAAA‑AGAAAAAGCAACAGGCAGGGAATAAGATTAAAGAAAATGTCGCTAAATTTaac                                                           >  1:531981/1‑149 (MQ=255)                                                                                                tGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAA‑TTTTTCCA‑ACAAAAAAAAAAACTGGAGTTGCCATGATGAGGGGAA‑GGAATAAGCAAAAGGCAGTGAATAAGGTGACAGAGATTTTCGCTAACTTTACCGcc                                                        >  1:245911/1‑151 (MQ=255)                                                                                                 ggCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑ATTTTTCAGACAAAAACAAAAAATGGAGTTGCCATGATGAGCGGAA‑GTAATAAACAACAAGCAGTGAATAAGATGACAGAGATTGTCGCTAAATTTAcc                                                           >  2:587180/1‑147 (MQ=255)                                                                                                  gCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTTCAGACAAATAAAAAAAATGGAGTTGCCATGATGAGCGAAA‑GTAATAAACAACAGGCAGTGAATAAATTGACAGAGATTGTCGCTAACTTTACCGcc                                                        >  1:894317/1‑149 (MQ=255)                                                                                                     aaaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTTCAGACAAAAAAAAAAACTGGAGTTGCCATGATGAGCGAAA‑GTAAAAAGCAACAGGCAGTGAATAAGATGACAGAGATTTTCGCTAACTTTTACGcc                                                        >  1:763337/1‑146 (MQ=255)                                                                                                      aaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTTCAGACAAAAACAAAAAATGGAGTTGCCATGATGAGCGGAA‑GAAATAAACAAAAGGCAGGGAATAAATTGAAAGAGATTGTTGCTAAATTTACCGCCATGAtt                                                  >  1:599663/1‑151 (MQ=255)                                                                                                      aaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTTCAAACACAAAAAAAAACTGGAGTTGTCATGATGAGCGGAA‑GTAAAAAGAAACAAGCAGAGAAAAAATTGTCAGAGATTGTCGCGAAATTTTTCGCCa                                                       >  2:524388/1‑146 (MQ=255)                                                                                                               aaCCACATTTTTAGTGGTAGTTTGTGGCGGTG‑GA‑TTTTTTCCGACAAAAAAAAAAAATGGAGGTGCCATGATGAGCGGAA‑AGAATAAACAAAAGGGAGGGAAAAAAGTGGCAGAGATTTTCGCTAAATTTACCGCCCTGATTTTTAccc                                           >  1:1093460/1‑149 (MQ=255)                                                                                                               aaCCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAA‑GTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGa                                         <  1:224710/151‑1 (MQ=255)                                                                                                                 ccACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTTCAGACAAAAAAAAAAACTTGAGTTGGCATGATGAGCGAAA‑GGAATAAACAACAGGCAGTGAATAAGGTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTTCCCGaa                                        >  1:1117075/1‑150 (MQ=255)                                                                                                                 ccACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAA‑GTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGaa                                        <  2:550263/150‑1 (MQ=255)                                                                                                                 ccACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTCCAGACAAATACAAAAACTGGAGTTGCAATGATGAGCGAAA‑GTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGaa                                        <  2:317355/150‑1 (MQ=255)                                                                                                                  cacaTTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTCCAGACAAATACAAAAAATGGAGTTGCCATGATGAGCGAAA‑GGAATAAACAACAAGCAGGGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGaa                                        >  1:244853/1‑149 (MQ=255)                                                                                                                     aTTTTTAGTGGTAGTTTGTGGCGGCG‑GA‑TTTTTTTAGACAAAAATCAAAAATGGAGGTGCCATGATGAGGGTGA‑GTAAAAAGGAAAAGGAAGTGAATAAGATGAAAGAGAATGTCGCTAACTTTACCGCCACGATTTTTACCCGAATGccg                                   >  2:530118/1‑150 (MQ=255)                                                                                                                            gTGGTAGTTTGTGGGGGTG‑AT‑TTTTCCCAGAAAAATCAAAAAACTGGAGTTGCCATGATGAGCGAAA‑GTAATAAGCAACAGGCAGTGAATAAGTGAACAGATTTTGCCCCTACCTTGACCGCCATGATTTCTACCCGAATGCCTGATGACg                            <  2:30300/151‑1 (MQ=255)                                                                                                                              ggTTGTTTGTGGGGGTG‑AA‑TTTTTCAAAAAAAAAACAAAATCTGGAGTTGCCATGAGGAGGGAAA‑GTAATAAGCAACAGGAGGTGAATAAGTTGACAGAGATTGTGGCTACCTTTACCGCCATGATTTCTACCCGAATGCCtgatg                               <  1:674861/146‑1 (MQ=255)                                                                                                                              ggTAGTTTGTGGCGGTG‑AA‑TTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAA‑GTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgt                           <  1:685487/150‑1 (MQ=255)                                                                                                                                 aGTTTGTGGCGGTA‑AA‑TTTTTCCAGACAAATACAAAAACTGGAGTTGCCAGGATGAGCGAAA‑GTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgtggt                        <  2:442196/150‑1 (MQ=255)                                                                                                                                  gTTTGTGGCGGTG‑AA‑TTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAA‑GTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgtggtg                       <  1:974293/150‑1 (MQ=255)                                                                                                                                      gtgGCGGTG‑AA‑TTTTTCCAGAAAAATACAAAAACTGGAGTTGCCATGATGAGCGAAA‑GTAATAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGCTATCTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATaa                  <  1:693452/151‑1 (MQ=255)                                                                                                                                        ggCGGTG‑AA‑TTTTTCCAGACAAATACAAAAACTGGGGTTGCCATGATGAGCGAAA‑GTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATGGTCGCAAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAAc                <  2:933070/151‑1 (MQ=255)                                                                                                                                           ggTG‑AA‑TTTTTCCAGACAAATAAAAAACCTGGAGTTGCCATGATGAGCGAAA‑GTAATAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTa              <  1:341369/150‑1 (MQ=255)                                                                                                                                            gTG‑AA‑TTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAA‑GTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaa             >  2:771892/1‑150 (MQ=255)                                                                                                                                                aa‑TTTTTCCAGACAAATAAAAAAACTGGAGTTGCCATGATGAGCGAAA‑GTAATAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACa         <  1:636376/151‑1 (MQ=255)                                                                                                                                                   tttttCCAGCCAAATACAAAAACTGGAGTTGCCATGAGTAGCAAAA‑GTAATAACCAACAGGCAGTAAATAGGTTGACGGAATTTGTCGCTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAg        <  2:861568/150‑1 (MQ=255)                                                                                                                                                    ttttCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAA‑GTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCt      >  2:607012/1‑151 (MQ=255)                                                                                                                                                    ttttCCAGAAAAAGACAAAAACTGGAGTTACCATGATAAGCAAAA‑TAAATAAGCAACAGCCAGTGAATAATTTGACAGGGATTGTCGCTAAGTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACa         <  2:400521/148‑1 (MQ=255)                                                                                                                                                     tttCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAA‑GTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCt      >  2:1107194/1‑150 (MQ=255)                                                                                                                                                       tCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAA‑GTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTaa    >  1:735199/1‑150 (MQ=255)                                                                                                                                                        ccAGACAAATACAAAAACTGGAGTTGCCAGGATGAGCGAAA‑GTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAg  <  2:809846/151‑1 (MQ=255)                                                                                                                                                        ccAGACAAATACAAAAACGGGAGTTGCCATGATGAGCGAAA‑GTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTACCTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTaaa   <  1:995231/150‑1 (MQ=255)                                                                                                                                                        |                                                                                                                                                        AGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AA‑TTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAA‑GTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG  >  NC_000913/3206286‑3206585

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑