Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913436,5160CA44.6% 75.7 / 35.6 65Q295K (CAA→AAA) thiLthiamine monophosphate kinase
Reads supporting (aligned to +/- strand):  ref base C (25/11);  new base A (0/29);  total (25/40)
Fisher's exact test for biased strand distribution p-value = 1.39e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01

TCTCGCCATGTTGAACCGGAACAGGCGCTGCGCTGGGCGCTCTCTGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTAC  >  NC_000913/436369‑436658
                                                                                                                                                      |                                                                                                                                              
tctcGCCATGTTGAACCGGAACAGGCGCTGCGCTGGGCGCTCTCTGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCaa                                                                                                                                              <  1:293451/150‑1 (MQ=255)
   cGCCATGTTGAACCGGAACAGGCGCTGCGCTGGGCGCTCTCTGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATg                                                                                                                                           <  1:1058498/150‑1 (MQ=255)
     ccATGTTGAACCGGAACAGGCGCTGCGCTGGGCGCTCTCTGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATGAcc                                                                                                                                        <  2:945922/151‑1 (MQ=255)
        tGTTGAACCGGAACAGGCGCTGCGCTGGGCGCTCTCTGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATGAccgc                                                                                                                                      <  2:167554/150‑1 (MQ=255)
          ttGAACCGGAACAGGCGCTGCGCTGGGCGCTCTCTGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATGAccgccg                                                                                                                                    >  2:212340/1‑150 (MQ=255)
            gacccGGAACGGGCGCTGCGCGGGGCCCTCTCTGGCGGTGAATATTACGATTTGTGTTTCCCTGTGCCGAAACTGAACCGTGGCGCGCTGGATGTGGCTCGGGGACCCCGGGGCGTACCTTTTCCTTTTACCGGGAAAATGACCGCCGata                                                                                                                                 <  2:50204/148‑1 (MQ=255)
                cGGAACAGGCGCTGCGCTGGGCGCTCTCTGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATGACCGCCGATATCg                                                                                                                              >  2:927790/1‑150 (MQ=255)
                cGGAACAGGCGCTGCGCTGGGCGCTCTCTGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATGACCGCCGATATCg                                                                                                                              >  1:571445/1‑150 (MQ=255)
                 ggAACGGGCGCTGCGCGGGGCGCTCTCGGGCGGTGAAGATTACGATTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCCCGGACACCTGGGCGTCCCGTTTCCCTGTATCGGGAAAATGACCGCCGATATCGaa                                                                                                                            <  2:748608/151‑1 (MQ=255)
                 ggAACAGGCGCTGCGCTGGGCGCTCTCTGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATGACCGCCGATATCGa                                                                                                                             >  2:236313/1‑150 (MQ=255)
                      aGGCGCTGCGCTGGGCGCTCTCTGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATGACCGCCGATATCGAAGGGCt                                                                                                                       >  1:1031557/1‑151 (MQ=255)
                      aGGCGCTGCGCTGGGCGCTCTCTGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATGACCGCCGATATCGAAGGGCt                                                                                                                       >  1:757921/1‑151 (MQ=255)
                      aGGCGCTGCGCTGGGCGCTCTCTGGCGGTGAAGATTACGAGTTGGGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAATTGACCGCCGATATCGAAGGGc                                                                                                                        >  2:228711/1‑150 (MQ=255)
                         cgctgcgcggGGCGCTCTGGGGGGGTGAAGATTAGGGTTTGTGTTTCCCTGTGCCGGAACGAACCGGGGGCGCGGGGGTTGTGCCTCTCGGCCACCGGGGCGTACCTTTTCCCTATATGGGGAAAATGACCGCCGATATCGAAGGGCt                                                                                                                       <  2:1055121/148‑1 (MQ=255)
                          gctgcgctgGGCGCTCTCTGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATGACCGCCGATATCGAAGGGCTTTGt                                                                                                                   >  2:749392/1‑151 (MQ=255)
                                  gggCCCTCTCTGGGGGTAAAGTTTACGGGTTGTTTTTCACTGTGCCGGAACTGACCCGGGGCCCGCTGGATGTGGCTCCCGGACCCCGGGGCTTCCCTTTTACCTGTATCGGGAAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTc                                                                                                            <  1:135401/150‑1 (MQ=255)
                                        tctctGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACgg                                                                                                     >  2:906508/1‑151 (MQ=255)
                                           ctGGGGGGGAGGATTAGGTGTTGTGTTTCCCTGTGCCGAAACTGAGCGGTGGCGCGTTGGATGTGGCTCTCGGACCCCGGGGCGTACCTTTTACCTGTGTGGGGCAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGc                                                                                                    <  2:120398/149‑1 (MQ=255)
                                            ggggggTGAAGATTACGGTTTGTTTTCCCCTGGCCCGAAACGGACCGGGGGCGCGTTGGAGGGGGCTCTGGCCCCCTTGGGCGTACCGTTTCCCTGAAACGGGAAAAGGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCg                                                                                                   <  2:926658/145‑1 (MQ=255)
                                              ggggTGAAGATTAGGTGTTTTGTTTCACTGGGCGGGAACTAAACCGTGGCGCGCTGGATGTGGCTCTCGGACCCCTGGGCTTCCCTTTTACCTGTATCGGGAAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAAcc                                                                                               <  2:266253/149‑1 (MQ=255)
                                                ggggAAGATGACGAGTGGTGTTTCACGGTGCCGGAACTGAACGGGGGCGCGGGGGATGGGGCTCTGGGCCACGTGGGTGTCCCGTTTCCTTTTATGGGGAAAAGGGCCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCt                                                                                              <  1:1111963/147‑1 (MQ=255)
                                                ggTGAAGATTACGAGTTGTTTTTCCCTGTGCGGGAACTGACCGGGGGCGCGGTGGATGTGGCTCTCGGACCCCGGGGCGTCCCGTTTCCTTTTATCGGCCAAAGGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCt                                                                                              <  1:621909/150‑1 (MQ=255)
                                                  tGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGtt                                                                                           >  1:716576/1‑151 (MQ=255)
                                                    aaGATTACGATTTGTTTTTCACTGTGCCGAAACGGACCCGTGGCGCGCTGGATGTGGCTCCCGGACCCCGGGGCGTCCCGTTTCCCTGTATCGGGAAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGtt                                                                                           <  1:515590/149‑1 (MQ=255)
                                                     aGATTAAGAGTTGTGTTTCACTGCGGCGAAACTGACCGGTGGCGCGCGAGGTGGGGCTCTGGGACCCTGGGGCGCACTGTTTACCTGTTGGGGACAAAGGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACa                                                                                        <  1:59802/151‑1 (MQ=255)
                                                          aCGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACAtt                                                                                      >  1:1028321/1‑148 (MQ=255)
                                                            gggttgttTTTCCCTGTGCCGGACCTGACCCGTGGCGCGTTGGATGGGCCTCTCGGACACCGGGGCGTCCCTTTTCCCTGTATCGGGAAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACt                                                                                 <  1:670498/149‑1 (MQ=255)
                                                               ttttttttCACGGTGCCGGACTTGACCCGGGGCGCGTTGGTTGTGGCTCTCGGACCCCTGGGCGTACCTTTTCCTTTTATCGGGAAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACt                                                                                 <  2:948680/143‑1 (MQ=255)
                                                                  tgtTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGaa                                                                             >  2:465832/1‑149 (MQ=255)
                                                                    tttcctggtgCCGGACTTGACCCGTGGCGCGTGGGATGTGGCTCTCGGACACCTGGGCGTCCCGTTTCCCTGTATGGGGAAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTgg                                                                               <  1:271838/138‑1 (MQ=255)
                                                                    tttCACTGTGCGGGAACTGAACGGGGGCGCGCTGGATGTGGCTCCCGGACCCTGGGGCGTCCCTTTTCCCTGTATCGGGCAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAgg                                                                          <  1:280288/150‑1 (MQ=255)
                                                                    ttccACGGTGCCAGAGGTGACCGGGGGCGCGCTGGATGTGGCTCCCGACCACCGGGCCGTGCCGTTACCCTGTATCGGCCAAAAGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGaaa                                                                            <  1:327185/145‑1 (MQ=255)
                                                                     ttCACTGTGCCGGAACTGACCCGTGGCGCGCTGGATGTGGCTCTGGGACCCGGGGGCGTCCCTTTTCCCTGTATGGGGAAAAGGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGa                                                                         <  1:1111720/150‑1 (MQ=255)
                                                                      tCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGat                                                                        >  1:737600/1‑150 (MQ=255)
                                                                          tgtgCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGAc                                                                   >  1:457499/1‑151 (MQ=255)
                                                                              ccGGAACTGACCCGTGGCGCGCTGGTTGTGGCTCTCGGACCCCGGGGCGTCCCGTTTCCCTTTATCGGGAAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCAtt                                                               <  1:1115512/151‑1 (MQ=255)
                                                                                gaagcgaacccGGGGGGCGTTGGGTGTGGCTCTCGCACGCGGGGGGGTACCTTTTCCCTGAACCGGGAAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCag                                                                <  2:939713/139‑2 (MQ=255)
                                                                                 gAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTg                                                            >  1:326143/1‑151 (MQ=255)
                                                                                      gacccGTGGCGCGTTGGTTGTGGCTCTCGGACCCCTGGGCGTCCCTTTTCCCTGTATCGGGAAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCa                                                         <  2:389783/146‑1 (MQ=255)
                                                                                        cccGGGGCGCGGTGGTGGTGCCTCGCGGACACTGGGGCGTACGGTTTCCCTGTAGCGGGAAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGcc                                                     <  2:1093971/150‑1 (MQ=255)
                                                                                        accggTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTCCCGTTTCCCTGTATCGGGAAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCAc                                                        <  1:1035972/145‑1 (MQ=255)
                                                                                         ccGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGcc                                                     >  2:1062202/1‑150 (MQ=255)
                                                                                            ggggggcgtgggATGGGGCACTCGGACCCCGGGGAGTCCCGTTTCCCTGTATGGGGAAAATGGCCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCa                                                         <  1:299615/134‑1 (MQ=255)
                                                                                              ggcgggtgggATGGGCCTCTCGCACCCTGGGGCTCCCGTTTTCCGTGTACGGGAAAAATACCCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATaaa                                                <  2:176882/143‑1 (MQ=255)
                                                                                                 gcgtggggtggggCTCTCGAACCCTGGGGCTCCCCTTTTCCCTGTAGGGGGAAAAGGCCCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGAt                                             <  2:380605/140‑1 (MQ=255)
                                                                                                 gcgcTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATaa                                                 >  2:1115403/1‑147 (MQ=255)
                                                                                                 gcgcTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATaa                                                 >  1:1067894/1‑147 (MQ=255)
                                                                                                    cTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTc                                          >  1:192499/1‑151 (MQ=255)
                                                                                                      ggATGTGCCTCTGGGACCCTGGGGCGTCCCGTTTCCCTGTTTCGGGAAAAGGCCCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTcgc                                        <  1:927519/151‑1 (MQ=255)
                                                                                                         tgtgGCTCCCGGCCCCCGGGGCGTTCGTTTTCCCGTGATGGGCAAAAGGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTcgc                                        <  2:979846/148‑1 (MQ=255)
                                                                                                          gggcctccgggACCCCGGGGCGTCCTGTTTCCTTTTAGCGGAAAAATGCCCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGa                                      <  1:778928/140‑1 (MQ=255)
                                                                                                              ctctGGGCCCCCGGGGCGTCCGGTTTCCTTGTAGCGGAAAAATCGCCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATg                                            <  1:898116/139‑1 (MQ=255)
                                                                                                               tctcGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCg                               >  2:688768/1‑151 (MQ=255)
                                                                                                                   ggccgcgtGGGCGTCCGTTTTCCCTGTTGCGGACAATGGGCCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCg                               <  2:600574/140‑1 (MQ=255)
                                                                                                                   ggcccccTGGGCTTCCCGTTTCCCTTTATGGGGCAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTg                           <  2:354781/146‑1 (MQ=255)
                                                                                                                   ggACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTg                           >  2:647741/1‑151 (MQ=255)
                                                                                                                      ccccggggCGTCCCTTTTACCTGTATCGGGAAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAg                        <  1:1085621/146‑1 (MQ=255)
                                                                                                                           gggCGTCCTTTTTCCTTGTACCGGGAAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAg                        <  1:693012/146‑1 (MQ=255)
                                                                                                                           gggCATCCCGTTTCCCTGTATGGGGAAAAGGCCCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCt                            <  2:585227/142‑1 (MQ=255)
                                                                                                                               gTACCGTTTACCTGTATCGGGCAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAAt               >  2:160422/1‑151 (MQ=255)
                                                                                                                               gTACCGTTTACCTGTATCGGGCAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAAt               >  1:150807/1‑151 (MQ=255)
                                                                                                                                    gTTTACCTGTATCGGGCAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTg          >  1:268578/1‑151 (MQ=255)
                                                                                                                                      tttccttGTATCGGGAAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTg          <  1:961773/144‑1 (MQ=255)
                                                                                                                                       ttccttttATCGGAAAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTg          <  1:687482/142‑1 (MQ=255)
                                                                                                                                             gTATCGGGCAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTAc  >  1:1089874/1‑151 (MQ=255)
                                                                                                                                                      |                                                                                                                                              
TCTCGCCATGTTGAACCGGAACAGGCGCTGCGCTGGGCGCTCTCTGGCGGTGAAGATTACGAGTTGTGTTTCACTGTGCCGGAACTGAACCGTGGCGCGCTGGATGTGGCTCTCGGACACCTGGGCGTACCGTTTACCTGTATCGGGCAAATGACCGCCGATATCGAAGGGCTTTGTTTTATTCGTGACGGCGAACCTGTTACATTAGACTGGAAAGGATATGACCATTTTGCCACGCCATAAAGATGTCGCGAAAAGTCGCCTGAAGATGAGTAATCCGTGGCATCTAC  >  NC_000913/436369‑436658

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: