Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,315,513 T→G N168H (AAC→CAC)  infB ← translation initiation factor IF‑2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,315,5130TG99.0% 338.0 / ‑5.0 98N168H (AAC→CAC) infBtranslation initiation factor IF‑2
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base G (57/40);  total (58/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01

TCAGTCCATTTGTTTTCTTCCGCCATACGACGTGCTTCTTCAGCAACGCGACGTGCTTCTTCTTCGAGTTTACGACGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCTGGGCGTTTTTAGTCATATCGTCTTGTTGATTGCTCACTTTGTCTTTTTCCGCAGCTTCACGTTTCGCTTGTTCAGCAGCTTCACGCTTAGCTTGTTCTGCGGCCTCACGTTCAGCTTTTTGTTGCGCCTCGCGTTTAGCCGATTCTTCTGCCTC  >  NC_000913/3315365‑3315660
                                                                                                                                                    |                                                                                                                                                   
tCAGTCCATTTGTTTTCTTCCGCCATACGACGTGCTTCTTCAGCAACGCGACGTGCTTCTTCTTCGAGTTTACGACGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCTGGGCGtgtt                                                                                                                                                   >  2:295656/1‑151 (MQ=255)
    tCCATTTGTTTTCTTCCGCCATACGACCTGCTTCTTCAGCAACGCGACGTGCTTCTTCTCCGAGCTTACGACGCGCTTCTCCTTCAGCTTTACGCTTGAGCTCTCCAGCTTCCTGCTCACGGCGGCATCTTTAAGCCTGGGCGtgttt                                                                                                                                                  >  2:253997/1‑148 (MQ=255)
     ccATTTGTTTTCTTCCGCCATACGACGTGCTTCTTCAGCAACGCGACGTGCTTCTTCTTCGAGTTTACGACGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCTGGGCGTGTTTAGt                                                                                                                                               <  2:457360/150‑1 (MQ=255)
         ttGTTTTCTTCCGCCATACGACGTGCTTCTTCAGCAACGCGACGTGCTTCTTCTTCGAGTTTACGACGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCTGGGCGTGTTTAGTCata                                                                                                                                           <  2:527206/150‑1 (MQ=255)
           gTTTTCTTCCGCCATACGACGTGCTTCTTCAGCAACGCGACGTGCTTCTTCTTCGAGTTTACGACGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCTGGGCGTGTTTAGTCATATc                                                                                                                                         <  2:823374/150‑1 (MQ=255)
            ttttCTTCCGCCATACGACGTGCTTCTTCAGCAACGCGACGTGCTTCTTCTTCGAGTTTACGACGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCTGGGCGTGTTTAGTCATATCGt                                                                                                                                       >  1:948468/1‑151 (MQ=255)
             tttCTTCCGCCATACGACGTGCTTCTTCAGCAACGCGACGTGCTTCTTCTTCGAGTTTACGACGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCTGGGCGTGTTTAGTCATATCGt                                                                                                                                       <  2:569128/150‑1 (MQ=255)
             tttCTTCCGCCATACGACGTGCTTCTTCAGCAACGCGACGTGCTTCTTCTTCGAGTTTACGACGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCTGGGCGTGTTTAGTCATATCGt                                                                                                                                       <  1:316486/150‑1 (MQ=255)
              ttcttcCGCCATACGACGTGCTTCTTCAGCAACGCGACGTGCTTCTTCTTCGAGTTTACGACGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCTGGGCGTGTTTAGTCATATCGTCt                                                                                                                                     >  2:364605/1‑151 (MQ=255)
               tcttcCGCCATACGACGTGCTTCTTCAGCAACGCGACGTGCTTCTTCTTCGAGTTTACGACGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTTAGCCTGGGCGTGTTTAGTCATATCGTCtt                                                                                                                                    >  1:582919/1‑151 (MQ=255)
                 ttcCGCCATACGACGTGCTTCTTCAGCAACGCGACGTGCTTCTTCTTCGAGTTTACGACGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCTGGGCGTGTTTTGTCATATCGTCttgt                                                                                                                                  >  1:1107649/1‑151 (MQ=255)
                    cGCCATACGACGTGCTTCTTCAGCAACGCGACGTGCTTCTTCTTCGAGTTTACGACGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCTGGGCGTGTTTAGTCATATCGTCTTGTTGa                                                                                                                               <  1:401432/151‑1 (MQ=255)
                    cGCCATACGACGTGCTTCTTCAGCAACGCGACGTGCTTCTTCTTCGAGTTTACGACGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCTGGGCGTGTTTAGTCATATCGTCTTGTTGa                                                                                                                               <  1:795980/151‑1 (MQ=255)
                      ccATACGACGTGCTTCTTCAGCAACGCGACGTGCTTCTTCTTCGAGTTTACGACGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCTGGGCGTGTTTAGTCATATCGTCTTGTTGAtt                                                                                                                             <  1:161435/151‑1 (MQ=255)
                        aTACGACGTGCTTCTTCAGCAACGCGACGTGCTTCTTCTTCGAGTTTACGACGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCTGGGCGGGTTTAGTCCTTTCGTCTTGTTGAtt                                                                                                                             >  2:178522/1‑149 (MQ=255)
                          acgacgTGCTTCTTCAGCAACGCGACGTGCTTCTTCTTCGAGTTTACGACGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCTGGGCGTGTTTAGTCATATCGTCTTGTTGATTGCt                                                                                                                          >  1:119831/1‑150 (MQ=255)
                            gacgTGCTTCTTCAGCAACGCGACGTGCTTCTTCTTCGAGTTTACGACGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCTGGGCGTGTTTAGTCATATCGTCTTGTTGATTGCTCAc                                                                                                                       >  1:671893/1‑151 (MQ=255)
                             acgTGCTTCTTCAGCAACGCGACGTGCTTCTTCTTCGAGTTTACGACGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCTGGGCGTGTTTAGTCATATCGTCTTGTTGATTGCTCACt                                                                                                                      <  2:1069464/151‑1 (MQ=255)
                             acgTGCTTCTTCAGCAACGCGACGTGCTTCTTCTTCGAGTTTACGACGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCTGGGCGGGGTTAGTCCTATCCTCTTGTTGATTGCTCACt                                                                                                                      >  1:811459/1‑151 (MQ=255)
                              cgTGCTTCTTCAGCAACGCGACGTGCTTCTTCTTCGAGTTTACGACGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCTGGGCGTGGTTAGGCATATCGTCTTGTTGATTTCTCACtt                                                                                                                     >  1:158208/1‑151 (MQ=255)
                                 gCTTCTTCAGCAACGCGACGTGCTTCTTCTTCGAGTTTACGACGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCTGGGCGTGTTTAGTCATATCGTCTTGTTGATTGCTCACTTTGt                                                                                                                  <  2:434759/151‑1 (MQ=255)
                                  cttcttCAGCAACGCGACGTGCTTCTTCTTCGAGTTTACGACGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCTGGGCGTGTTTAGTCATATCGTCTTGTTGATTGCTCACTTTGt                                                                                                                  >  2:726398/1‑150 (MQ=255)
                                   ttcttcAGCAACGCGACGTGCTTCTTCTTCGAGTTTACGACGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCTGGGCGTGTTTAGTCATATCGTCTTGTTGATTGCTCACTTTGt                                                                                                                  >  1:661177/1‑149 (MQ=255)
                                    tcttcAGCAACGCGACGTGCTTCTTCTTCGAGTTTACGACGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCATCCTGGGCGCGTTTAGTCCTATCGTTTTGCTGTTTGTTCCCTTTGTCtt                                                                                                               >  1:1102647/1‑151 (MQ=255)
                                    tcttcAGCAACGCGACGTGCTTCTTCTTCGAGTTTACGACGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCTGGGCGTGTTTAGTCATATCGTCTTGTTGATTGCTCACTTTGTCt                                                                                                                <  2:646417/150‑1 (MQ=255)
                                       tcAGCAACGCGACGTGCTTCTTCTTCGAGTTTACGACGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCTGGGCGTGTTTAGTCATATCGTCTTGTTGATTGCTCACTTTGTCttt                                                                                                              <  1:952221/149‑1 (MQ=255)
                                        cAGCAACGCGACGTGCTTCTTCTTCGAGTTTACGACGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCTGGGCGTGTTTAGTCATATCGTCTTGTTGATTGCTCACTTTGTCttttt                                                                                                            <  2:145645/150‑1 (MQ=255)
                                        cAGCAACGCGACGTGCTTCTTCTTCGAGTTTACGACGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCTGGGCGTGTTTAGTCATATCGTCTTGTTGATTGCCCACTTTGTCttttt                                                                                                            >  2:564196/1‑150 (MQ=255)
                                         aGCAACGCGACGTGCTTCTTCTTCGAGTTTACGACGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCTGGGCGTGTTTAGTCATATCGTCTTGTTGATTGCTCACTTTGTCTTTTTcc                                                                                                          >  1:557125/1‑151 (MQ=255)
                                         aGCAACGCGACGTGCTTCTTCTTCGAGTTTACGACGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCTGGGCGTGTTTAGTCATATCGTCTTGTTGATTGCTCACTTTGTCTTTTTcc                                                                                                          <  2:908719/151‑1 (MQ=255)
                                            aaCGCGACGTGCTTCTTCTTCGAGTTTACGACGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCTGGGCGTGTTTAGTCATATCGTCTTGTTGATTGCTCACTTTGTCTTTTTCCGCa                                                                                                       <  1:948731/151‑1 (MQ=255)
                                             aCGCGACGTGCTTCTTCTTCGAGTTTACGACGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCTGGGCGTGTTTAGTCATATCGTCTTGTTGATTGCTCACTTTGTCTTTTTCCGCAg                                                                                                      <  2:11345/151‑1 (MQ=255)
                                             aCGCGACGTGCTTCTTCTTCGAGTTTACGACGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCTGGGCGTGTTTAGTCATATCGTCTTGTTGATTGCTCACTTTGTCTTTTTCCGCAg                                                                                                      <  1:535185/151‑1 (MQ=255)
                                              cgcgACGTGCTTCTTCTTCGAGTTTACGACGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCTGGGCGTGTTTAGTCATATCGTCTTGTTGATTGCTCACTTTGTCTTTTTCCGCAGc                                                                                                     >  1:603570/1‑151 (MQ=255)
                                              cgcgACGTGCTTCTTCTTCGAGTTTACGACGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCTGGGCGTGTTTAGTCATATCGTCTTGTTGATTGCTCACTTTGTCTTTTTCCGCAGc                                                                                                     >  2:507890/1‑151 (MQ=255)
                                               gcgACGTGCTTCTTCTTCGAGTTTACGACGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCTGGGCGTGTTTAGTCATATCGTCTTGTTGATTGCTCACTTTGTCTTTTTCCGCAGCt                                                                                                    <  2:725461/151‑1 (MQ=255)
                                                 gACGTGCTTCTTCTTCGAGTTTACGACGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCTGGGCGTGTTTAGTCATATCGTCTTTTTGATTGCTCACTTTGTCTTTTTCCGCAGCtt                                                                                                   >  2:676209/1‑150 (MQ=255)
                                                  aCGTGCTTCTTCTTCGAGTTTACGACGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCTGGGCGTGTTTAGTCATATCGTCTTGTTGATTGCTCACTTTGTCTTTTTCCGCAGCTTCa                                                                                                 <  2:1030329/151‑1 (MQ=255)
                                                   cGTGCTTCTTCTTCGAGTTTACGACGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCTGGGCGTGTTTAGTCATATCGTCTTGTTGATTGCTCACTTTGTCTTTTTCCGCAGCTTCAc                                                                                                >  1:765043/1‑151 (MQ=255)
                                                    gTGCTTCTTCTTCGAGTTTACGACGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCTGGGCGTGTTTAGTCATATCGTCTTGTTGATTGCTCACTTTGTCTTTTTCCGCAGCTTCACg                                                                                               <  2:404027/151‑1 (MQ=255)
                                                           ttcttcGAGTTTACGACGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCTGGGCGTGTTTAGTCATATCGTCTTGTTGATTGCTCACTTTGTCTTTTTCCGCAGCTTCACGttt                                                                                            >  2:629174/1‑147 (MQ=255)
                                                            tcttcGAGTTTACGACGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCTGGGCGTGTTTAGTCATATCGTCTTGTTGATTGCTCACTTTGTCTTTTTCCGCAGCTTCACGTTTCGCt                                                                                        <  1:674146/150‑1 (MQ=255)
                                                               tcGAGTTTACGACGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCTGGGCGTGTTTAGTCATATCGTCTTGTTGATTGCTCACTTTGTCTTTTTCCGCAGCTTCACGTTTCGCTTGtt                                                                                    >  1:463975/1‑151 (MQ=255)
                                                                 gAGTTTACGACGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCTGGGCGTGTTTAGTCATATCGTCTTGTTGATTGCTCACTTTGTCTTTTTCCGCAGCTTCACGTTTCGCTTGTTca                                                                                  <  1:665761/151‑1 (MQ=255)
                                                                 gAGTTTACGACGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCTGGGCGTGTTTAGTCATATCGTCTTGTTGATTGCTCACTTTGTCTTTTTCCCCAGCTTCCCGTTTCGCTTGtt                                                                                    >  2:63469/1‑149 (MQ=255)
                                                                  aGTTTACGACGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGGCTGGGGGTGTTTAGTCATATCGTCTTGTTGATTGCTCACTTTGTCTTTTTCCGCAGCTTTACGTTTCGCTTGtt                                                                                    >  1:393156/1‑148 (MQ=255)
                                                                  aGTTTACGACGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCTGGGCGTGTTTAGTCATATCGTCTTGTTGATTGCTCACTTTGTCTTTTTCCGCAGCTTCACGTTTCGCTTGtt                                                                                    >  2:554147/1‑148 (MQ=255)
                                                                       acgacgCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCTGGGCGTGTTTAGTCATATCGTCTTGTTGATTGCTCACTTTGTCTTTTTCCGCAGCTTCACGTTTCGCTTGTTCAGCAGCt                                                                            >  1:472444/1‑151 (MQ=255)
                                                                         gacgCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTTAGCCTGGGCGTGTTTAGGCATATCGTTTTTTTGGTTGCTCACTTTTTCTTTTTCCGCAGCTTCACGTTTTGCCTGTTCAGCAGCtt                                                                           >  2:1087766/1‑150 (MQ=255)
                                                                            gcgcTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCTGGGCGTGTTTAGTCATATCGTCTTGTTGATTGCTCACTTTGTCTTTTTCCGCAGCTTCACGTTTCGCTTGTTCAGCAGCTTCAc                                                                        >  2:660810/1‑150 (MQ=255)
                                                                             cgcTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCTGGGCGTGTTTAGTCATATCGTCTTGTTGATTGCTCACTTTGTCTTTTTCCGCAGCTTCACGTTTCGCTTGTTCAGCAGCTTCAc                                                                        >  2:225048/1‑149 (MQ=255)
                                                                                ttcttcttcAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCTGGGCGGGTTTAGTCATATCGTCTTGTTGATTGCTCCCTTTGTCTTTTTCCCCAGCTTCACGTTTCGCTTGTTCAGCAGCTTCACGCtt                                                                    >  1:1090879/1‑150 (MQ=255)
                                                                                 tcttcttcAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCTGGGCGTGTTTAGTCATATCGTCTTGTTGTTTGCTCACTTTGTCTTTTTCCGCAGCTTCACGTTTCGCTTGTTCAGCAGCTTCACGCtt                                                                    >  2:996405/1‑149 (MQ=255)
                                                                                    tcttcAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCTGGGGGCTTTTAGTCATATCGTCTTGTTGATTGCTCACTTTGTCTTTTTCCGCAGCTTCACGTTTCGCTTGTTCAGCAGCTTCACGCTTAGGtt                                                               >  1:143307/1‑151 (MQ=255)
                                                                                    tcttcAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCTGGGCGTGTTTAGTCATATCGTCTTGTTGATTGCTCACTTTGTCTTTTTCCGCAGCTTCACGTTTCGCTTGTTCAGCAGCTTCACGCTTAGCtt                                                               >  1:338980/1‑151 (MQ=255)
                                                                                       tcAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCTGGGCGTGTTTAGTCATATCGTCTTGTTGATTGCTCACTTTGTCTTTTTCCGCAGCTTCACGTTTCGCTTGTTCAGCAGCTTCACGCTTAGCTTGt                                                             <  2:180100/150‑1 (MQ=255)
                                                                                         aGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCTGGGCGTGTTTAGTCCTATCGTCTTGTTGATTGCTCCCTTTGTCTTTTTCCCCCGCTTCACGTTTCGCTTGTTCAGCAGCTTCACGCTTTGCTTGtt                                                            >  2:665823/1‑149 (MQ=255)
                                                                                         aGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCTGGGCGTGTTTAGTCATATCGTCTTGTTGATTGCTCACTTTGTCTTTTTCCGCAGCTTCACGTTTCGCTTGTTCAGCAGCTTCACGCTTAGCTTGTTCt                                                          <  1:542516/151‑1 (MQ=255)
                                                                                         aGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCTGGGCGTGTTTAGTCATATCGTCTTGTTGATTGCTCACTTTGTCTTTTTCCGCAGCTTCACGTTTCGCTTGTTCAGCAGCTTCACGCTTAGCTTGTTCt                                                          <  1:453830/151‑1 (MQ=255)
                                                                                          gCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCTGGGCGTGTTTAGTCATATCGTCTTGTTGATTGCTCACTTTGTCTTTTTCCGCAGCTTCACGTTTCGCTTGTTCAGCAGCTTCACGCTTAGCTTGTTCt                                                          >  2:408896/1‑150 (MQ=255)
                                                                                          gCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCTGGGCGTGTTTAGTCATATCGTCTTGTTGATTGCTCACTTTGTCTTTTTCCGCAGCTTCACGTTTCGCTTGTTCAGCAGCTTCACGCTTAGCTTGTTCt                                                          >  1:901192/1‑150 (MQ=255)
                                                                                             ttACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCTGGGCGTGTTTAGTCATATCGTCTTGTTGATTGCTCACTTTGTCTTTTTCCGCAGCTTCACGTTTCGCTTGTTCAGCAGCTTCACGCTTAGCTTGTTCTGCg                                                       <  1:333398/150‑1 (MQ=255)
                                                                                             ttACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCTGGGCGCGTTTAGGCATATCGTCTTGTTGATTTCTCACTTTGTTTTTTTCCGCAGCTTTACGTTTCGCTTGTTCAGCAGCTTCCCGCTTAGCTTGTTCTGCgg                                                      >  2:436335/1‑151 (MQ=255)
                                                                                              tACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCTGGGCGGGTTTTGTCATATCGGCTTGTTGATTGCTCCCTTTGTCTTTTTCCGCAGCTTCACGTTTCGCTTGTTCAGCAGCTTCACGCTTAGCTTGTTCTGCgg                                                      >  2:973625/1‑150 (MQ=255)
                                                                                               aCGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCTGGGCGTGTTTAGTCATATCGTCTTGTTGATTGCTCACTTTGTCTTTTTCCGCAGCTTCACGTTTCGCTTGTTCAGCAGCTTCACGCTTAGCTTGTTCTGCGGc                                                     <  1:436948/150‑1 (MQ=255)
                                                                                                 gCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCTGGGCGTGTTTAGTCATATCGTCTTGTTGATTGCTCACTTTGTCTTTTTCCGCAGCTTCACGTTTCGCTTGTTCAGCAGCTTCACGCTTAGCTTGTTCTGCGGCCTc                                                  <  1:352904/151‑1 (MQ=255)
                                                                                                   ttGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCTGGGCGTGTTTAGTCATATCGTCTTGTTGGTTGCTTACTTTGTCTTTTTCCGCCGCTTTACGTTTTGCTTGTTCCGCAGCTTTACGCTTTGCTTGTTCTGCCGCCTCAc                                                >  2:640975/1‑151 (MQ=255)
                                                                                                   ttGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCTGGGCGGGTTTAGTCATATCGTCTTGTTGATTGCTCACTTTGTCTTTTTCCGCAGCTTCACGTTTCGCTTGTTCAGCAGCTTCACGCTTAGCTTGTTCTGCGGCCTc                                                  >  2:118538/1‑149 (MQ=255)
                                                                                                    tGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCTGGGCGTGTTTAGTCATATCGTCTTGTTGATTGCTCACTTTGTCTTTTTCCGCAGCTTCACGTTTCGCTTGTTCAGCAGCTTCACGCTTAGCTTGTTCTGCGGCCTCAc                                                <  2:1058416/150‑1 (MQ=255)
                                                                                                      aGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCTGGGCGTGTTTAGTCATATCGTCTTGTTGATTGCTCACTTTGTCTTTTTCCGCAGCTTCACGTTTCGCTTGTTCAGCAGCTTCACGCTTTGCTTGTTCTGCGGCCTCACGtt                                             >  2:631446/1‑151 (MQ=255)
                                                                                                      aGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCTGGGCGTGTTTAGTCATATCGTCTTGTTGATTGCTCACTTTGTCTTTTTCCGCAGCTTCACGTTTCGCTTGTTCAGCAGCTTCACGCTTAGCTTGTTCTGCGGCCTCACGtt                                             <  1:335297/151‑1 (MQ=255)
                                                                                                         tctGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCTGGGCGTGTTTAGTCATATCGTCTTGTTGATTGCTCACTTTGTCTTTTTCCGCAGCTTCACGTTTCGCTTGTTTAGCAGCTTCACGCTTAGCTTGTTCTGCGGCCTCACg                                               >  2:772939/1‑146 (MQ=255)
                                                                                                         tctGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCTGGGCGTGTTTAGTCATATCGTCTTGTTGATTGCTCACTTTGTCTTTTTCCGCAGCTTCACGTTTCGCTTGTTCAGCAGCTTCACGCTTAGCTTGTTCTGCGGCCTCACGtt                                             >  1:806545/1‑148 (MQ=255)
                                                                                                         tctGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCCGGGCGTGTTTAGTCATATCGGCCTGTTGATTGCTCTCCTTGTCTTTTTCCGCAGCTTCACGTTTCGACTGTTCAGCAGCTTTACGCCTTGCTTGTTCTGCGGCCGCACGtt                                             >  2:1012081/1‑148 (MQ=255)
                                                                                                            gCAGCTTCCTGCTCACGGCCGGCTTTTTCAGCCTGGCCGCGTTTAGTCATATTGTCTTGTTGATTGCTCCCTTTGTCTTTTTCCGCCGCTTCCCGTTTCGCTTGTTCTGCAGCTTCACGCTTAGCTTGTTCTGCGGCCTCACGTTCa                                           >  2:471894/1‑147 (MQ=255)
                                                                                                              aGCTTCCTGCTCACGGCGGGCTTTTTCAGCCTGGGCGTGTTTAGTCATATCGTCTTGTTGATTGCTCACTTTGTCTTTTTCCGCAGCTTCACGTTTCGCTTGTTCAGCAGCTTCACGCTTAGCTTGTTCTGCGGCCTCACGTTCAGCtttt                                     >  1:214779/1‑151 (MQ=255)
                                                                                                                cTTCCTGCTCACGGCGGGCTTTTTCAGCCTGGGCGTGTTTAGTCATATCGTCTTGTTGATTGCTCACTTTGTCTTTTTCCGCAGCTTCACGTTTCGCTTGTTCAGCAGCTTCACGCTTAGCTTGTTCTGCGGCCTCACGTTCAGCttttt                                    >  1:966924/1‑150 (MQ=255)
                                                                                                                  tCCTGCTCACGGCGGGCTTTTTCAGCCTGGGCGTGTTTAGTCATATCGTCTTGTTGATTGCTCACTTTGTCTTTTTCCGCAGCTTCACGTTTCGCTTGTTCAGCAGCTTCACGCTTAGCTTGTTCTGCGGCCTCACGTTCAGCTTTttgt                                  <  1:202819/150‑1 (MQ=255)
                                                                                                                   ccTGCTCACGGCGGGCTTTTTCAGCCTGGGCGTGTTTAGTCATATCGTCTTGTTGATTGCTCACTTTGTCTTTTTCCGCAGCTTCACGTTTCGCTTGTTCAGCAGCTTCACGCTTAGCTTGTTCTGCGGCCTCACGTTCAGCTTTttgttg                                <  2:1102488/151‑1 (MQ=255)
                                                                                                                   ccTGCTCACGGCGGGCTTTTTCAGCCTGGGCGTGTTTAGTCATATCGTCTTGTTGATTGCTCACTTTGTCTTTTTCCGCAGCTTCACGTTTCGCTTGTTCAGCAGCTTCACGCTTAGCTTGTTCTGCGGCCTCACGTTCAGCTTTttgtt                                 <  2:108048/150‑1 (MQ=255)
                                                                                                                   ccTGCTCACGGCGGGCTTTTTCAGCCTGGGCGTGTTTAGTCATATCGTCTTGTTGATTGCTCACTTTGTCTTTTTCCGCAGCTTCACGTTTCGCTTGTTCAGCAGCTTCACGCTTAGCTTGTTCTGCGGCCTCACGTTCAGCTTTttgtt                                 <  1:209902/150‑1 (MQ=255)
                                                                                                                     tGCTCACGGCGGGCTTTTTCAGCCTGGGCGTGTTTAGTCATATCGTCTTGTTGATTGCTCACTTTGTCTTTTTCCGCAGCTTCACGTTTCGCTTGTTCAGCAGCCTCACGCTTAGCTTGTTCTGCGGCCTCACGTTCAGCTTTttgtt                                 >  1:114290/1‑148 (MQ=255)
                                                                                                                      gCTCACGGCGGGCTTTTTCAGCCTGGGCGTGTTTAGTCATATCGTCTTGTTGATTGCTCACTTTGTCTTTTTCCGCAGCTTCACGTTTCGCTTGTTCAGCAGCTTCACGCTTAGCTTGTTCTGCGGCCTCACGTTCAGCTTTTTGTTgc                               <  2:877348/149‑1 (MQ=255)
                                                                                                                        tCACGGCGGGCTTTTTCAGCCTGGGCGTGTTTAGTCATATCGTCTTGTTGATTGCTCACTTTTTCTTTTTTCGCAGCTTCACGTTTCGCTTGTTCAGCAGCTTCACGCTTAGCTTGTTCTGCGGCCTCACGTTCAGCTTTTTGTTGCGcc                            >  2:390199/1‑150 (MQ=255)
                                                                                                                        tCACGGCGGGCTTTTTCAGCCTGGGCGTGTTTAGTCATATCGTCTTGTTGATTGCTCACTTTGTCTTTTTCCGCAGCTTCACGTTTCGCTTGTTCAGCAGCTTCACGCTTAGCTTGTTCTGCGGCCTCACGTTCAGCTTTTTGTTGCGcc                            >  2:853499/1‑150 (MQ=255)
                                                                                                                         cACGGCGGGCTTTTTCAGCCTGGGCGTGTTTAGTCATATCGTCTTGTTGATTGCTCACTTTGTCTTTTTCCGCAGCTTCACGTTTCGCTTGTTCAGCAGCTTCACGCTTAGCTTGTTCTGCGGCCTCACGTTCAGCTTTTTGTTGCGCCTc                          >  1:1012461/1‑151 (MQ=255)
                                                                                                                           cggcggGCTTTTTCAGCCTGGGCGTGTTTAGTCATATCGTCTTGTTGATTGCTCACTTTGTCTTTTTCCGCAGCTTCACGTTTCGCTTGTTCAGCAGCTTCACGCTTAGCTTGTTCTGCGGCCTCACGTTCAGCTTTTTGTTGCGCCTcg                         <  1:99090/150‑1 (MQ=255)
                                                                                                                           cggcggGCTTTTTCAGCCTGGGCGTGTTTAGTCATATCGTCTTGTTGATTGCTCACTTTGTCTTTTTCCGCAGCTTCACGTTTCGCTTGTTCAGCAGCTTCACGCTTAGCTTGTTCTGCGGCCTCACGTTCAGCTTTTTGTTGCGCCTcg                         <  2:615036/150‑1 (MQ=255)
                                                                                                                            ggcggGCTTTTTCAGCCTGGGCGTGTTTAGTCATATCGTCTTGTTGATTGCTCACTTTGTCTTTTTCCGCAGCTTCACGTTTCGCTTGTTCAGCAGCTTCACGCTTAGCTTGTTCTGCGGCCTCACGTTCAGCTTTTTGTTGCGCCTcgcg                       >  2:888826/1‑151 (MQ=255)
                                                                                                                             gcggGCTTTTTCAGCCTGGGCGTGTTTAGTCATATCGTCTTGTTGATTGCTCACTTTGTCTTTTTCCGCAGCTTCACGTTTCGCTTGTTCAGCAGCTTCACGCTTAGCTTGTTCTGCGGCCTCACGTTCAGCTTTTTGTTGCGCCTCGCGt                      >  1:282458/1‑151 (MQ=255)
                                                                                                                                ggCTTTTTCAGCCTGGGCGTGTTTAGTCATATCGTCTTGTTGATTGCTCACTTTGTCTTTTTCCGCAGCTTCACGTTTCGCTTGTTCAGCAGCTTCACGCTTAGCTTGTTCTGCGGCCTCACGTTCAGCTTTTTGTTGCGCCTCGCGTTTa                   <  2:335080/151‑1 (MQ=255)
                                                                                                                                    ttttCAGCCTGGGCGTGTTTAGTCATATCGTCTTGTTGATTGCTCACTTTGTCTTTTTCCGCAGCTTCACGTTTCGCTTGTTCAGCAGCTTCACGCTTAGCTTGTTCTGCGGCCTCACGTTCAGCTTTTTGTTGCGCCTCGCGTTTAGc                 <  2:360989/149‑1 (MQ=255)
                                                                                                                                     tttCAGCCTGGGCGTGTTTAGTCATATCGTCTTGTTGATTGCTCACTTTGTCTTTTTCCGCAGCTTCACGTTTCGCTTGTTCAGCAGCTTCACGCTTAGCTTGTTCTGCGGCCTCACGTTCAGCTTTTTGTTGCGCCTCGCGTTTAGCCg               >  2:80051/1‑150 (MQ=255)
                                                                                                                                      ttCAGCCTGGGCGTGTTTAGTCATATCGTCTTGTTGATTGCTCACTTTGTCTTTTTCCGCAGCTTCACGTTTCGCTTGTTCAGCAGCTTCACGCTTAGCTTGTTCTGCGGCCTCACGTTCAGCTTTTTGTTGCGCCTCGCGTTTAGCCGAt             >  2:744218/1‑151 (MQ=255)
                                                                                                                                      ttCAGCCTGGGCGTGTTTAGTCATATCGTCTTGTTGATTGCTCACTTTGTCTTTTTCCGCAGCTTCACGTTTCGCTTGTTCAGCAGCTTCACGCTTAGCTTGTTCTGCGGCCTCACGTTCAGCTTTTTGTTGCGCCTCGCGTTTAGCCGAt             >  2:922653/1‑151 (MQ=255)
                                                                                                                                         aGCCTGGGCGTGTTTAGTCATATCGTCTTGTTGATTGCTCACTTTGTCTTTTTCCGCAGCTTCACGTTTCGCTTGTTCAGCAGCTTCACGCTTAGCTTGTTCTGCGGCCTCACGTTCAGCTTTTTGTTGCGCCTCGCGTTTAGCCGAttct          >  1:297239/1‑151 (MQ=255)
                                                                                                                                           ccTGGGCGTGTTTAGTCATATCGTCTTGTTGATTGCTCACTTTGTCTTTTTCCGCAGCTTCACGTTTCGCTTGTTCAGCAGCTTCACGCTTAGCTTGTTCTGCGGCCTCACGTTCAGCTTTTTGTTGCGCCTCGCGTTTAGCCGAttcttc        >  2:248261/1‑151 (MQ=255)
                                                                                                                                              gggCGTGTTTAGTCATATCGTCTTGTTGATTGCTCACTTTGTCTTTTTCCGCAGCTTCACGTTTCGCTTGTTCAGCAGCTTCACGCTTAGCTTGTTCTGCGGCCTCACGTTCAGCTTTTTGTTGCGCCTCGCGTTTAGCCGATTCTTCTg      <  2:700451/150‑1 (MQ=255)
                                                                                                                                                gCGTGTTTAGTCATATCGTCTTGTTGATTGCTCACTTTGTCTTTTTCCGCAGCTTCACGTTTCGCTTGTTCAGCAGCTTCACGCTTAGCTTGTTCTGCGGCCTCACGTTCAGCTTTTTGTTGCGCCTCGCGTTTAGCCGATTCTTCTGCCt   >  1:633242/1‑151 (MQ=255)
                                                                                                                                                 cGTGTTTAGTCATATCGTCTTGTTGATTGCTCACTTTGTCTTTTTCCGCAGCTTCACGTTTCGCTTGTTCAGCAGCTTCACGCTTAGCTTGTTCTGCGGCCTCACGTTCAGCTTTTTGTTGCGCCTCGCGTTTAGCCGATTCTTCTGCCTc  >  1:1116604/1‑151 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TCAGTCCATTTGTTTTCTTCCGCCATACGACGTGCTTCTTCAGCAACGCGACGTGCTTCTTCTTCGAGTTTACGACGCGCTTCTTCTTCAGCTTTACGCTTGAGCTCTGCAGCTTCCTGCTCACGGCGGGCTTTTTCAGCCTGGGCGTTTTTAGTCATATCGTCTTGTTGATTGCTCACTTTGTCTTTTTCCGCAGCTTCACGTTTCGCTTGTTCAGCAGCTTCACGCTTAGCTTGTTCTGCGGCCTCACGTTCAGCTTTTTGTTGCGCCTCGCGTTTAGCCGATTCTTCTGCCTC  >  NC_000913/3315365‑3315660

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: