Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,725,175	C→G	E48D (GAG→GAC)	glyQ ←	glycine tRNA synthetase, alpha subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,725,175	0	C	G	100.0%	336.1 / NA	87	E48D (GAG→GAC)	glyQ	glycine tRNA synthetase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (49/38);  total (49/38)

CAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTA  >  NC_000913/3725028‑3725322                                                                                                                                                    |                                                                                                                                                    cAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTcg                                                                                                                                                  >  2:335156/1‑151 (MQ=255) cAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTcg                                                                                                                                                  >  1:918056/1‑151 (MQ=255)  aGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTcgc                                                                                                                                                 >  2:1102838/1‑151 (MQ=255)  aGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTcgc                                                                                                                                                 >  1:194412/1‑151 (MQ=255)    cTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCgcgc                                                                                                                                               >  2:492851/1‑151 (MQ=255)      ccTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAg                                                                                                                                             <  1:978247/151‑1 (MQ=255)        tGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCAACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGAc                                                                                                                                           >  2:228378/1‑151 (MQ=255)         gAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGAc                                                                                                                                           >  2:267026/1‑150 (MQ=255)         gAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGATTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACa                                                                                                                                          <  1:524197/151‑1 (MQ=255)          aaTATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAg                                                                                                                                         <  2:493760/151‑1 (MQ=255)            tatTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGt                                                                                                                                       >  1:876598/1‑151 (MQ=255)             atTGTCCGGCGACGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGCAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTc                                                                                                                                      >  2:901786/1‑151 (MQ=255)              ttGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGt                                                                                                                                       <  1:62680/149‑1 (MQ=255)                gTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCAt                                                                                                                                    <  1:1058438/150‑1 (MQ=255)                  ccGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTgg                                                                                                                                 >  1:103536/1‑151 (MQ=255)                  ccGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTgg                                                                                                                                 <  1:261504/151‑1 (MQ=255)                       gATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTg                                                                                                                              >  1:427762/1‑149 (MQ=255)                       gATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTg                                                                                                                              >  1:5772/1‑149 (MQ=255)                        aTGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTgaga                                                                                                                           <  2:523904/151‑1 (MQ=255)                        aTGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTg                                                                                                                              >  1:491327/1‑148 (MQ=255)                         tGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTgag                                                                                                                            >  2:39065/1‑149 (MQ=255)                               aaTGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACTTAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTccc                                                                                                                    <  2:769551/151‑1 (MQ=255)                                  gACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCcgc                                                                                                                  <  1:250389/150‑1 (MQ=255)                                    ccaccTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCgcgc                                                                                                                <  2:754811/150‑1 (MQ=255)                                     caccTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCg                                                                                                              <  2:51852/151‑1 (MQ=255)                                        cTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACt                                                                                                           >  1:1033266/1‑151 (MQ=255)                                          ggAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACt                                                                                                           <  1:401829/149‑1 (MQ=255)                                            aaCTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTcc                                                                                                        >  2:974827/1‑150 (MQ=255)                                              cTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATg                                                                                                     >  1:466992/1‑151 (MQ=255)                                                gATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTc                                                                                                   >  1:247100/1‑151 (MQ=255)                                                  tagtagTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTcc                                                                                                  >  1:521440/1‑150 (MQ=255)                                                   agtagtGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCa                                                                                                 >  1:834512/1‑150 (MQ=255)                                                        tGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGtt                                                                                           >  1:1114621/1‑151 (MQ=255)                                                            gTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGaa                                                                                        <  1:750405/150‑1 (MQ=255)                                                             tAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAAc                                                                                       >  2:649740/1‑150 (MQ=255)                                                              aaaCGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACaa                                                                                     <  2:9244/151‑1 (MQ=255)                                                              aaaCGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACaa                                                                                     <  2:56623/151‑1 (MQ=255)                                                                 cGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATgg                                                                                  <  1:610598/151‑1 (MQ=255)                                                                  ggTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATgg                                                                                  >  2:803606/1‑150 (MQ=255)                                                                  ggTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGt                                                                                 >  2:177132/1‑151 (MQ=255)                                                                   gTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTg                                                                                <  1:328534/151‑1 (MQ=255)                                                                    ttGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGc                                                                               >  1:457711/1‑151 (MQ=255)                                                                      ggggTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGc                                                                               >  2:1114951/1‑149 (MQ=255)                                                                       gggTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGc                                                                            >  1:155086/1‑151 (MQ=255)                                                                        ggTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGcc                                                                           >  2:158717/1‑151 (MQ=255)                                                                           tttCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCt                                                                         <  2:613640/150‑1 (MQ=255)                                                                             tCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGc                                                                      >  1:599240/1‑151 (MQ=255)                                                                               gCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGGGAGAGGCTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGc                                                                      >  2:205460/1‑149 (MQ=255)                                                                                ccGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCg                                                                     >  1:868997/1‑149 (MQ=255)                                                                                 cGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGc                                                                  >  2:721155/1‑151 (MQ=255)                                                                                 cGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGc                                                                  >  1:47172/1‑151 (MQ=255)                                                                                   tAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGccc                                                                >  2:787882/1‑151 (MQ=255)                                                                                    aGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGccc                                                                >  2:991344/1‑150 (MQ=255)                                                                                    aGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGccc                                                                <  2:1037450/150‑1 (MQ=255)                                                                                    aGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCa                                                               <  1:880260/151‑1 (MQ=255)                                                                                     gCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGccc                                                                <  2:233062/149‑1 (MQ=255)                                                                                         ccATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTaa                                                           <  2:676106/150‑1 (MQ=255)                                                                                         ccATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAAt                                                          >  1:717424/1‑151 (MQ=255)                                                                                               gtcggGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTa                                                    <  1:864948/151‑1 (MQ=255)                                                                                                  gggCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGTCTTCCATGTCCAATGGTTGAACAATGGCGCAGCCCTGGCGAGCCCAGTAATCCTGTAAgg                                                 >  2:673694/1‑151 (MQ=255)                                                                                                  gggCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAgg                                                 <  2:26516/151‑1 (MQ=255)                                                                                                   ggCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGt                                                <  2:103512/151‑1 (MQ=255)                                                                                                     cgacgaGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTc                                               >  2:500878/1‑150 (MQ=255)                                                                                                      gacgaGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTc                                               >  1:753172/1‑149 (MQ=255)                                                                                                       acgaGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAgg                                            >  1:107261/1‑151 (MQ=255)                                                                                                        cgaGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAgg                                            <  1:77503/150‑1 (MQ=255)                                                                                                          agaAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGACCAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATc                                         >  2:526755/1‑151 (MQ=255)                                                                                                           gaAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATc                                         >  1:907639/1‑150 (MQ=255)                                                                                                                 tGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCt                                  >  1:976336/1‑151 (MQ=255)                                                                                                                    aCATAAGCAGCCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGa                               <  1:340829/151‑1 (MQ=255)                                                                                                                           cagcCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCt                        <  2:319811/151‑1 (MQ=255)                                                                                                                            agcCGCCATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTg                       <  2:490749/151‑1 (MQ=255)                                                                                                                              ccgccATCGGTTCTGGCCCCAGGTCGCGCAGACCGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGt                     <  1:65112/151‑1 (MQ=255)                                                                                                                                gccATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTAt                   >  2:496241/1‑151 (MQ=255)                                                                                                                                gccATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTAt                   <  2:5772/151‑1 (MQ=255)                                                                                                                                 ccATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTAt                   <  2:300836/150‑1 (MQ=255)                                                                                                                                  cATCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCa                 <  2:804813/151‑1 (MQ=255)                                                                                                                                   aTCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCa                 >  1:296786/1‑150 (MQ=255)                                                                                                                                   aTCGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCa                 >  1:1106229/1‑150 (MQ=255)                                                                                                                                     cGGTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAAc              <  1:96931/151‑1 (MQ=255)                                                                                                                                       gTTCTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACtt            >  2:610757/1‑151 (MQ=255)                                                                                                                                          cTGGCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTg         >  1:972557/1‑151 (MQ=255)                                                                                                                                            ggCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACtttt          <  1:259297/148‑1 (MQ=255)                                                                                                                                            ggCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGc        >  2:309702/1‑150 (MQ=255)                                                                                                                                            ggCCCCAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGc        <  2:880146/150‑1 (MQ=255)                                                                                                                                               cccAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCatat    <  2:876462/151‑1 (MQ=255)                                                                                                                                                 cAGGTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCAtatta  <  2:752314/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                    CAGCTCCTGAATATTGTCCGGCGATGGCTTAATGACCACCTGGAACTGATAGTAGTGCTGTAAACGGTTGGGGTTTTCGCCGTAGCGACCATCGGTCGGGCGACGAGAAGGCTGAACATAAGCAGCCGCCATCGGTTCTGGCCCCAGCTCGCGCAGACAGGTCATTGGGTGAGAGGTTCCCGCGCCGACTTCCATGTCCAATGGTTGAACAATGGTGCAGCCCTGGCGAGCCCAGTAATCCTGTAAGGTCAGGATCAAGCCCTGGAAGGTCCTGGTATCAAACTTTTGCATATTA  >  NC_000913/3725028‑3725322

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑